Strain Name: |
STOCK Rag1tm1Mom Tg(TIE2GFP)287Sato/J |
|---|---|
Stock Number: |
006770 |
Availability: | Repository- Live |
General Terms and Conditions |
| Genes & Alleles | GFP; Rag1; Rag1tm1Mom; Tek; Tg(TIE2GFP)287Sato; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Targeted Mutation Type JAX® GEMM® Strain - Transgenic Mating System See Colony Maintenance (Female x Male) Species laboratory mouse Donating Investigator IMR Colony, The Jackson Laboratory Generation N1F4 (24-JUN-08) Strain Description
To generate this double mutant strain, B6.Cg-Tg(TIE2GFP)287Sato/1J (Stock No. 004659) was crossed to C.129S7(B6)-Rag1tm1Mom/J (Stock No. 003145). This mutant mouse strain may be useful in studies examining angiogenesis in transplanted tissues.In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described for each single mutant. We will modify the strain description if necessary as published results become available.
Strain Development
For the Rag1tm1Mom targeted mutation, a replacement targeting vector with the Pgk-neo marker was used. Homologous recombination of the targeting vector resulted in a 1356 bp deletion in the 5' end of the coding sequence. The 129S7/SvEvBrd AB1 ES cell line was used. The BALB/c congenic strain was generated by Dr. Bob Coffman by backcrossing mice carrying the Rag1tm1Mom mutation seven times to BALB/cAnNTac inbred mice.The Tg(TIE2GFP)287Sato transgenic mutation originated on an FVB/N background, and was backcrossed to C57BL/6J for five generations using a speed congenic protocol. The C57BL/6J component of this speed congenic background is estimated at >95% via genome scanning.
To generate this double mutant strain, B6.Cg-Tg(TIE2GFP)287Sato/1J (Stock No. 004659) was crossed to C.129S7(B6)-Rag1tm1Mom/J (Stock No. 003145).
Gene & Allele Details
| Allele Symbol | Rag1tm1Mom | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Peter Mombaerts | ||
| Common Name(s) | RAG-1-; RAG1null; Rag-; Rag-1KO; Rag1-; | ||
| Mutation Made By | Peter Mombaerts, Max Planck Institute of Biophysics | ||
| Strain of Origin | 129S7/SvEvBrd-Hprt1<+> | ||
| ES Cell Line Name | AB1 | ||
| ES Cell Line Strain | 129S7/SvEvBrd-Hprt1<+> | ||
| Gene Symbol and Name | Rag1, recombination activating gene 1 | ||
| Chromosome | 2 | ||
| Gene Common Name(s) | MGC43321; RNF74; Rag-1; | ||
| Molecular Note | A 1356 bp genomic fragment of the Rag1 gene, encoding the nuclear localization signal and the zinc-finger motif, was replaced by a neomycin cassette. A mutant transcript expressed from this allele was detected by Northern blot in bone marrow derived cell lines from homozygous mice. [MGI Ref ID J:1934] [MGI Ref ID J:96036] | ||
| Allele Symbol | Tg(TIE2GFP)287Sato | ||
| Allele Name | transgene insertion 287, Thomas N Sato | ||
| Common Name(s) | TIE2-GFP; | ||
| Mutation Made By | Thomas Sato, Weill Medical College of Cornell Univ | ||
| Site of Expression | Blood vessel endothelial cells fluoresce green allowing the visualization of all vessels under fluorescent microscopy or purification of endothelial cells by FACS. | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | Tek, endothelial-specific receptor tyrosine kinase, mouse, laboratory | ||
| General Note | Endothelial cells in transgenic mice express GFP, and can be visualized via fluorescent microscopy or purified by FACS. | ||
| Molecular Note | The transgene contains Green Fluorescent Protein (GFP) under the control of the endothelial specific receptor tyrosine kinase (Tek, previous symbol Tie2) promoter. [MGI Ref ID J:93360] | ||
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, these mice can be bred as homozygous for the Rag1tm1Mom allele and hemizygous for the Tg(TIE2GFP)287Sato transgene. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (146 strains)
004659 B6.Cg-Tg(TIE2GFP)287Sato/1J 003658 STOCK Tg(TIE2GFP)287Sato/J
002194 B6;CBA-Tg(CD3E)26Cpt/J 002506 STOCK Tg(CD3E)26Cpt-Rag1tm1Mom/J
004128 B6.Cg-Tg(Tek-cre)12Flv/J 002856 FVB/N-Tg(TIE2-lacZ)182Sato/J 005707 STOCK Rag1tm1Mom Tg(TIE2-lacZ)182Sato/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
GFP relatedResearch Tools
Cancer Research (endothelial cell marker for neovascularization)
Cardiovascular Research (endothelial cell marker for neovascularization)
Fluorescent Proteins
Genetics Research (Tissue/Cell Markers: endothelial cell markers for neovascularization)
Rag1tm1Mom relatedResearch Tools
Fluorescent Proteins
Cancer Research
Toxicology (B and T cell deficiency) (xenograft/transplant host)
Hematological Research
Immunological Defects (B and T cell deficiency)
Immunology and Inflammation Research
Immunodeficiency (B and T cell deficiency)
Inflammation (B and T cell deficiency)
T Cell Receptor Signaling Defects (B and T cell deficiency)
Internal/Organ Research
Lymphoid Tissue Defects (B and T cell deficiency)
Research Tools
Cancer Research (B and T cell deficiency) (xenograft/transplant host)
Toxicology Research (xenograft/transplant host)
References
Additional ReferencesPrice and Supply Information
| Strain Name: | STOCK Rag1tm1Mom Tg(TIE2GFP)287Sato/J |
| Stock Number: | 006770 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
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For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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