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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Hemizygote (Female x Male) 08-AUG-07 Species laboratory mouse Generation N5?+F10N (18-JAN-11)
Generation DefinitionsDonating Investigator Talal Chatila, David Geffen School of Medicine, UCLA Description
Homozygous mice are viable and fertile with normal T and B cell development. These "Foxp3EGFP" mice co-express EGFP and the regulatory T cell-specific transcription factor Foxp3 under the control of the endogenous promoter. EGFP expression accurately identifies the Foxp3+ T cell population (more than 97% of Foxp3+ T cells were EGFP+), and Foxp3 mRNA expression strictly segregates with EGFP+ T cells. Due to X-inactivation in females, the number of EGFP+CD4+ T cells found in the peripheral blood of heterozygous females was approximately half that of hemizygote males. CD4+EGFP+ cells also exhibit normal regulatory T cell suppression of effector cell proliferation (following stimulation with anti-CD3 and anti-CD28 monclonal antibodies ). Some EGFP expression is noted in a small population of CD8+ thymocytes. These mutant mice may be useful in immunological studies, including studies of regulatory T cell proliferation, localization, and antigen independence during the primary immune response.In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This allele was originally published on a BALB/c genetic background (see Stock No. 006769). It should be noted that the phenotype could vary from that originally described. The strain description will be modified as published results become available.
Development
A targeting vector was designed to place an IRES-EGFP-SV40 polyA sequence immediately downstream of the endogenous Foxp3 translational stop codon but upstream of the endogenous polyA signal. This also placed a loxP-flanked PGKneo cassette into intron 9. This vector was electroporated into 129X1Sv/J-derived SCC10 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and chimeric males were mated with C57BL/6 females. The PGKneo cassette was removed by mating founder males with Cre-deleter female mice on a C57BL/6 congenic background. The resulting mice (now harboring this Foxp3EGFP allele) were backcrossed to C57BL/6NCrl for 2 generations by Dr. Talal Chatila (UCLA) and then sent to Dr. Timothy Ley (Washington University). There, mice were further backcrossed 3 generations to C57BL/6J using a genomic screening (speed congenics) approach. The resulting mice (with 98-99% identity to C57BL/6J) were sent back to Dr. Chatila at UCLA prior to arrival at The Jackson Laboratory.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (345 strains)
006769 C.Cg-Foxp3tm2Tch/J
004088 B6.Cg-Foxp3sf/J 014579 B6.NOD-Tg(Foxp3-EGFP/cre)1aJbs/J 006769 C.Cg-Foxp3tm2Tch/J 008374 C57BL/6-Foxp3tm1Flv/J 006775 NOD.Cg-Foxp3sf/DoiJ 008694 NOD/ShiLt-Tg(Foxp3-EGFP/cre)1cJbs/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Foxp3tm2Tch/Foxp3+
C.129X1-Foxp3tm2Tch
- immune system phenotype
- *normal* immune system phenotype
Foxp3tm2Tch/Y
C.129X1-Foxp3tm2Tch
- immune system phenotype
- *normal* immune system phenotype
This mouse can be used to support research in many areas including:
GFP relatedImmunology and Inflammation Research
Immunodeficiency
T cell deficiency
Research Tools
Fluorescent Proteins
Genetics Research
Tissue/Cell Markers
Tissue/Cell Markers: T cell specific surface marker
Immunology and Inflammation Research
T cell specific surface marker
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Foxp3tm2Tch | ||
|---|---|---|---|
| Allele Name | targeted mutation 2, Talal A Chatila | ||
| Allele Type | Targeted (Reporter) | ||
| Common Name(s) | Foxp3-GFP KI; Foxp3EGFP; Foxp3IRES-GFP; Foxp3tm2(EGFP)Tch; | ||
| Mutation Made By | Talal Chatila, David Geffen School of Medicine, UCLA | ||
| Strain of Origin | 129X1/SvJ | ||
| ES Cell Line Name | SCC10 | ||
| ES Cell Line Strain | 129X1/SvJ | ||
| Site of Expression | EGFP is expressed in the Foxp3+ T cell population. Some EGFP expression is noted in a small population of CD8+ thymocytes. | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Molecular Note | An IRES-EGFP-SV40 poly A sequence was inserted immediately downstream of the endogenous Foxp3 translational stop codon, but upstream of the endogenous polyA signal, generating a bicistronic locus encoding both Foxp3 and EGFP. Expression of EGFP is restricted to the T cell lineage, primarily to the CD4+ T cell population. [MGI Ref ID J:120713] | ||
| Gene Symbol and Name | Foxp3, forkhead box P3 | ||
| Chromosome | X | ||
| Gene Common Name(s) | AIID; DIETER; IPEX; JM2; PIDX; RGD1562112; XPID; scurfin; scurfy; sf; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Foxp3tm2Tch, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Haribhai D; Lin W; Relland LM; Truong N; Williams CB; Chatila TA. 2007. Regulatory T cells dynamically control the primary immune response to foreign antigen. J Immunol 178(5):2961-72. [PubMed: 17312141] [MGI Ref ID J:120713]
Lin W; Haribhai D; Relland LM; Truong N; Carlson MR; Williams CB; Chatila TA. 2007. Regulatory T cell development in the absence of functional Foxp3. Nat Immunol 8(4):359-68. [PubMed: 17273171] [MGI Ref ID J:120636]
Additional References
Foxp3tm2Tch relatedAtarashi K; Tanoue T; Shima T; Imaoka A; Kuwahara T; Momose Y; Cheng G; Yamasaki S; Saito T; Ohba Y; Taniguchi T; Takeda K; Hori S; Ivanov II; Umesaki Y; Itoh K; Honda K. 2011. Induction of colonic regulatory T cells by indigenous Clostridium species. Science 331(6015):337-41. [PubMed: 21205640] [MGI Ref ID J:172265]
Cai SF; Cao X; Hassan A; Fehniger TA; Ley TJ. 2010. Granzyme B is not required for regulatory T cell-mediated suppression of graft-versus-host disease. Blood 115(9):1669-77. [PubMed: 19965675] [MGI Ref ID J:157876]
Cavassani KA; Carson WF 4th; Moreira AP; Wen H; Schaller MA; Ishii M; Lindell DM; Dou Y; Lukacs NW; Keshamouni VG; Hogaboam CM; Kunkel SL. 2010. The post sepsis-induced expansion and enhanced function of regulatory T cells create an environment to potentiate tumor growth. Blood 115(22):4403-11. [PubMed: 20130237] [MGI Ref ID J:161572]
Chen C; Liu Y; Liu Y; Zheng P. 2010. Mammalian target of rapamycin activation underlies HSC defects in autoimmune disease and inflammation in mice. J Clin Invest 120(11):4091-101. [PubMed: 20972332] [MGI Ref ID J:167563]
Chen X; Das R; Komorowski R; Beres A; Hessner MJ; Mihara M; Drobyski WR. 2009. Blockade of interleukin-6 signaling augments regulatory T-cell reconstitution and attenuates the severity of graft-versus-host disease. Blood 114(4):891-900. [PubMed: 19491393] [MGI Ref ID J:150726]
Choi J; Ritchey J; Prior JL; Holt M; Shannon WD; Deych E; Piwnica-Worms DR; DiPersio JF. 2010. In vivo administration of hypomethylating agents mitigate graft-versus-host disease without sacrificing graft-versus-leukemia. Blood 116(1):129-39. [PubMed: 20424188] [MGI Ref ID J:162801]
Chougnet CA; Tripathi P; Lages CS; Raynor J; Sholl A; Fink P; Plas DR; Hildeman DA. 2011. A major role for Bim in regulatory T cell homeostasis. J Immunol 186(1):156-63. [PubMed: 21098226] [MGI Ref ID J:168007]
Chung HS; Lee JH; Kim H; Lee HJ; Kim SH; Kwon HK; Im SH; Bae H. 2010. Foxp3 is a novel repressor of microglia activation. Glia 58(10):1247-56. [PubMed: 20544860] [MGI Ref ID J:168043]
Cong Y; Feng T; Fujihashi K; Schoeb TR; Elson CO. 2009. A dominant, coordinated T regulatory cell-IgA response to the intestinal microbiota. Proc Natl Acad Sci U S A 106(46):19256-61. [PubMed: 19889972] [MGI Ref ID J:154759]
Feng T; Cong Y; Qin H; Benveniste EN; Elson CO. 2010. Generation of mucosal dendritic cells from bone marrow reveals a critical role of retinoic Acid. J Immunol 185(10):5915-25. [PubMed: 20944006] [MGI Ref ID J:165627]
Hams E; McCarron MJ; Amu S; Yagita H; Azuma M; Chen L; Fallon PG. 2011. Blockade of B7-H1 (programmed death ligand 1) enhances humoral immunity by positively regulating the generation of T follicular helper cells. J Immunol 186(10):5648-55. [PubMed: 21490158] [MGI Ref ID J:173223]
Haribhai D; Williams JB; Jia S; Nickerson D; Schmitt EG; Edwards B; Ziegelbauer J; Yassai M; Li SH; Relland LM; Wise PM; Chen A; Zheng YQ; Simpson PM; Gorski J; Salzman NH; Hessner MJ; Chatila TA; Williams CB. 2011. A requisite role for induced regulatory T cells in tolerance based on expanding antigen receptor diversity. Immunity 35(1):109-22. [PubMed: 21723159] [MGI Ref ID J:174377]
Kaminitz A; Yolcu ES; Askenasy EM; Stein J; Yaniv I; Shirwan H; Askenasy N. 2011. Effector and Naturally Occurring Regulatory T Cells Display No Abnormalities in Activation Induced Cell Death in NOD Mice. PLoS One 6(6):e21630. [PubMed: 21738739] [MGI Ref ID J:174547]
Lathrop SK; Bloom SM; Rao SM; Nutsch K; Lio CW; Santacruz N; Peterson DA; Stappenbeck TS; Hsieh CS. 2011. Peripheral education of the immune system by colonic commensal microbiota. Nature 478(7368):250-4. [PubMed: 21937990] [MGI Ref ID J:177128]
Liu Z; Falo LD Jr; You Z. 2011. Knockdown of HMGB1 in tumor cells attenuates their ability to induce regulatory T cells and uncovers naturally acquired CD8 T cell-dependent antitumor immunity. J Immunol 187(1):118-25. [PubMed: 21642542] [MGI Ref ID J:176186]
Mashayekhi M; Sandau MM; Dunay IR; Frickel EM; Khan A; Goldszmid RS; Sher A; Ploegh HL; Murphy TL; Sibley LD; Murphy KM. 2011. CD8alpha(+) dendritic cells are the critical source of interleukin-12 that controls acute infection by Toxoplasma gondii tachyzoites. Immunity 35(2):249-59. [PubMed: 21867928] [MGI Ref ID J:176232]
Mayne CG; Spanier JA; Relland LM; Williams CB; Hayes CE. 2011. 1,25-Dihydroxyvitamin D3 acts directly on the T lymphocyte vitamin D receptor to inhibit experimental autoimmune encephalomyelitis. Eur J Immunol 41(3):822-32. [PubMed: 21287548] [MGI Ref ID J:175424]
Morlacchi S; Soldani C; Viola A; Sarukhan A. 2011. Self-antigen presentation by mouse B cells results in regulatory T-cell induction rather than anergy or clonal deletion. Blood 118(4):984-91. [PubMed: 21652680] [MGI Ref ID J:174859]
Niess JH; Adler G. 2010. Enteric flora expands gut lamina propria CX3CR1+ dendritic cells supporting inflammatory immune responses under normal and inflammatory conditions. J Immunol 184(4):2026-37. [PubMed: 20089703] [MGI Ref ID J:159471]
Pallotta MT; Orabona C; Volpi C; Vacca C; Belladonna ML; Bianchi R; Servillo G; Brunacci C; Calvitti M; Bicciato S; Mazza EM; Boon L; Grassi F; Fioretti MC; Fallarino F; Puccetti P; Grohmann U. 2011. Indoleamine 2,3-dioxygenase is a signaling protein in long-term tolerance by dendritic cells. Nat Immunol 12(9):870-8. [PubMed: 21804557] [MGI Ref ID J:176469]
Patterson SJ; Han JM; Garcia R; Assi K; Gao T; O'Neill A; Newton AC; Levings MK. 2011. Cutting edge: PHLPP regulates the development, function, and molecular signaling pathways of regulatory T cells. J Immunol 186(10):5533-7. [PubMed: 21498666] [MGI Ref ID J:173220]
Procaccini C; De Rosa V; Galgani M; Abanni L; Cali G; Porcellini A; Carbone F; Fontana S; Horvath TL; La Cava A; Matarese G. 2010. An oscillatory switch in mTOR kinase activity sets regulatory T cell responsiveness. Immunity 33(6):929-41. [PubMed: 21145759] [MGI Ref ID J:167293]
Rivas EI; Driver JP; Garabatos N; Presa M; Mora C; Rodriguez F; Serreze DV; Stratmann T. 2011. Targeting of a T cell agonist Peptide to lysosomes by DNA vaccination induces tolerance in the nonobese diabetic mouse. J Immunol 186(7):4078-87. [PubMed: 21346228] [MGI Ref ID J:170837]
Schallenberg S; Tsai PY; Riewaldt J; Kretschmer K. 2010. Identification of an immediate Foxp3(-) precursor to Foxp3(+) regulatory T cells in peripheral lymphoid organs of nonmanipulated mice. J Exp Med 207(7):1393-407. [PubMed: 20584884] [MGI Ref ID J:163388]
Tessmer MS; Reilly EC; Brossay L. 2011. Salivary gland NK cells are phenotypically and functionally unique. PLoS Pathog 7(1):e1001254. [PubMed: 21249177] [MGI Ref ID J:172475]
Venet F; Chung CS; Huang X; Lomas-Neira J; Chen Y; Ayala A. 2009. Lymphocytes in the development of lung inflammation: a role for regulatory CD4+ T cells in indirect pulmonary lung injury. J Immunol 183(5):3472-80. [PubMed: 19641139] [MGI Ref ID J:151875]
Yokosuka T; Kobayashi W; Takamatsu M; Sakata-Sogawa K; Zeng H; Hashimoto-Tane A; Yagita H; Tokunaga M; Saito T. 2010. Spatiotemporal basis of CTLA-4 costimulatory molecule-mediated negative regulation of T cell activation. Immunity 33(3):326-39. [PubMed: 20870175] [MGI Ref ID J:164647]
Zhang W; Zhang D; Shen M; Liu Y; Tian Y; Thomson AW; Lee WP; Zheng XX. 2010. Combined administration of a mutant TGF-beta1/Fc and rapamycin promotes induction of regulatory T cells and islet allograft tolerance. J Immunol 185(8):4750-9. [PubMed: 20844194] [MGI Ref ID J:164884]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry This mutant allele is located on the X chromosome. When maintaining a live colony, homozygous females are bred with hemizygous males. Mating System Homozygote x Hemizygote (Female x Male) 08-AUG-07 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $172.00 Male Hemizygous for Foxp3tm2Tch $172.00 Female Homozygous for Foxp3tm2Tch
Pairs /Price (US dollars $) Pair Genotype $344.00 Homozygous for Foxp3tm2Tch x Hemizygous for Foxp3tm2Tch Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $223.60 Male Hemizygous for Foxp3tm2Tch $223.60 Female Homozygous for Foxp3tm2Tch
Pairs /Price (US dollars $) Pair Genotype $447.20 Homozygous for Foxp3tm2Tch x Hemizygous for Foxp3tm2Tch Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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