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- Description
- Phenotype
- Genes & alleles
- Genotyping
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Description
Strain Information
Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Hemizygote (Female x Male) 08-AUG-07 Species laboratory mouse Generation N5?+F5 (28-OCT-08) Donating Investigator Talal Chatila, David Geffen School of Medicine, UCLA Description
Homozygous mice are viable and fertile with normal T and B cell development. These "Foxp3EGFP" mice co-express EGFP and the regulatory T cell-specific transcription factor Foxp3 under the control of the endogenous promoter. EGFP expression accurately identifies the Foxp3+ T cell population (more than 97% of Foxp3+ T cells were EGFP+), and Foxp3 mRNA expression strictly segregates with EGFP+ T cells. Due to X-inactivation in females, the number of EGFP+CD4+ T cells found in the peripheral blood of heterozygous females was approximately half that of hemizygote males. CD4+EGFP+ cells also exhibit normal regulatory T cell suppression of effector cell proliferation (following stimulation with anti-CD3 and anti-CD28 monclonal antibodies ). Some EGFP expression is noted in a small population of CD8+ thymocytes. These mutant mice may be useful in immunological studies, including studies of regulatory T cell proliferation, localization, and antigen independence during the primary immune response.In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This allele was originally published on a BALB/c genetic background (see Stock No. 006769). It should be noted that the phenotype could vary from that originally described. The strain description will be modified as published results become available.
Development
A targeting vector was designed to place an IRES-EGFP-SV40 polyA sequence immediately downstream of the endogenous Foxp3 translational stop codon but upstream of the endogenous polyA signal. This also placed a loxP-flanked PGKneo cassette into intron 9. This vector was electroporated into 129X1Sv/J-derived SCC10 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and chimeric males were mated with C57BL/6 females. The PGKneo cassette was removed by mating founder males with Cre-deleter female mice on a C57BL/6 congenic background. The resulting mice (now harboring this Foxp3EGFP allele) were backcrossed to C57BL/6NCrl for 2 generations by Dr. Talal Chatila (UCLA) and then sent to Dr. Timothy Ley (Washington University). There, mice were further backcrossed 3 generations to C57BL/6J using a genomic screening (speed congenics) approach. The resulting mice (with 98-99% identity to C57BL/6J) were sent back to Dr. Chatila at UCLA prior to arrival at The Jackson Laboratory.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (225 strains)
006769 C.Cg-Foxp3tm2Tch/J
004088 B6.Cg-Foxp3sf/J 008374 C57BL/6-Foxp3tm1Flv/J 006775 NOD.Cg-Foxp3sf/DoiJ 008694 NOD/ShiLt-Tg(Foxp3-EGFP/cre)1Jbs/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Foxp3tm2Tch/Foxp3+
C.129X1-Foxp3tm2Tch
- immune system phenotype
- *normal* immune system phenotype (MGI Ref ID J:120713)
- females are phenotypically normal and indistinguishable from wild-type littermates
- EGFP expression in cells concordant with Foxp3 expression allows the kinetics of regulatory T cell proliferation and localization during a primary immune response
Foxp3tm2Tch/Y
C.129X1-Foxp3tm2Tch
- immune system phenotype
- *normal* immune system phenotype (MGI Ref ID J:120713)
- males are phenotypically normal and indistinguishable from wild-type littermates
- EGFP expression in cells concordant with Foxp3 expression allows the kinetics of regulatory T cell proliferation and localization during a primary immune response
This mouse can be used to support research in many areas including:
Immunology and Inflammation Research
Immunodeficiency
T cell deficiency
Research Tools
Fluorescent Proteins
Genetics Research
Tissue/Cell Markers: T cell specific surface marker
Immunology and Inflammation Research
T cell specific surface marker
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Foxp3tm2Tch | ||
|---|---|---|---|
| Allele Name | targeted mutation 2, Talal A Chatila | ||
| Allele Type | Targeted (Reporter) | ||
| Common Name(s) | Foxp3EGFP; Foxp3tm2(EGFP)Tch; | ||
| Mutation Made By | Talal Chatila, David Geffen School of Medicine, UCLA | ||
| Strain of Origin | 129X1/SvJ | ||
| ES Cell Line Name | SCC10 | ||
| ES Cell Line Strain | 129X1/SvJ | ||
| Site of Expression | EGFP is expressed in the Foxp3+ T cell population. Some EGFP expression is noted in a small population of CD8+ thymocytes. | ||
| Gene Symbol and Name | Foxp3, forkhead box P3 | ||
| Chromosome | X | ||
| Gene Common Name(s) | AIID; DIETER; IPEX; JM2; MGC141961; MGC141963; PIDX; RGD1562112; XPID; scurfin; scurfy; sf; | ||
| Molecular Note | An IRES-EGFP-SV40 poly A sequence was inserted immediately downstream of the endogenous Foxp3 translational stop codon, but upstream of the endogenous polyA signal, generating a bicistronic locus encoding both Foxp3 and EGFP. Expression of EGFP is restricted to the T cell lineage, primarily to the CD4+ T cell population. [MGI Ref ID J:120713] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Foxp3tm2Tch, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Haribhai D; Lin W; Relland LM; Truong N; Williams CB; Chatila TA. 2007. Regulatory T cells dynamically control the primary immune response to foreign antigen. J Immunol 178(5):2961-72. [PubMed: 17312141] [MGI Ref ID J:120713]
Lin W; Haribhai D; Relland LM; Truong N; Carlson MR; Williams CB; Chatila TA. 2007. Regulatory T cell development in the absence of functional Foxp3. Nat Immunol 8(4):359-68. [PubMed: 17273171] [MGI Ref ID J:120636]
Additional References
Foxp3tm2Tch relatedChen X; Das R; Komorowski R; Beres A; Hessner MJ; Mihara M; Drobyski WR. 2009. Blockade of interleukin-6 signaling augments regulatory T-cell reconstitution and attenuates the severity of graft-versus-host disease. Blood 114(4):891-900. [PubMed: 19491393] [MGI Ref ID J:150726]
Venet F; Chung CS; Huang X; Lomas-Neira J; Chen Y; Ayala A. 2009. Lymphocytes in the development of lung inflammation: a role for regulatory CD4+ T cells in indirect pulmonary lung injury. J Immunol 183(5):3472-80. [PubMed: 19641139] [MGI Ref ID J:151875]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry This mutant allele is located on the X chromosome. When maintaining a live colony, homozygous females are bred with hemizygous males. Mating System Homozygote x Hemizygote (Female x Male) 08-AUG-07 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $160.40 Male Hemizygous for Foxp3tm2Tch $160.40 Female Homozygous for Foxp3tm2Tch
Pairs /Price (US dollars $) Pair Genotype $320.80 Homozygous for Foxp3tm2Tch x Hemizygous for Foxp3tm2Tch
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $208.60 Male Hemizygous for Foxp3tm2Tch $208.60 Female Homozygous for Foxp3tm2Tch
Pairs /Price (US dollars $) Pair Genotype $417.10 Homozygous for Foxp3tm2Tch x Hemizygous for Foxp3tm2Tch
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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