Strain Name: |
B6.Cg-Foxp3tm2Tch/J |
|---|---|
Stock Number: |
006772 |
Availability: | Repository- Live |
Price and Supply Information | |
General Terms and Conditions |
| Strain Common Names | B6.Cg-Foxp3tm2(EGFP)Tch/J; |
| Genes & Alleles | Foxp3; Foxp3tm2Tch; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Mating System Homozygote x Hemizygote (Female x Male) Species laboratory mouse Donating Investigator Talal Chatila, David Geffen School of Medicine, UCLA Generation N5?+F3 (28-DEC-07) Strain Description
Homozygous mice are viable and fertile with normal T and B cell development. These "Foxp3EGFP" mice co-express EGFP and the regulatory T cell-specific transcription factor Foxp3 under the control of the endogenous promoter. EGFP expression accurately identifies the Foxp3+ T cell population (more than 97% of Foxp3+ T cells were EGFP+), and Foxp3 mRNA expression strictly segregates with EGFP+ T cells. Due to X-inactivation in females, the number of EGFP+CD4+ T cells found in the peripheral blood of heterozygous females was approximately half that of hemizygote males. CD4+EGFP+ cells also exhibit normal regulatory T cell suppression of effector cell proliferation (following stimulation with anti-CD3 and anti-CD28 monclonal antibodies ). Some EGFP expression is noted in a small population of CD8+ thymocytes. These mutant mice may be useful in immunological studies, including studies of regulatory T cell proliferation, localization, and antigen independence during the primary immune response.In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This allele was originally published on a BALB/c genetic background (see Stock No. 006769). It should be noted that the phenotype could vary from that originally described. The strain description will be modified as published results become available.
Strain Development
A targeting vector was designed to place an IRES-EGFP-SV40 polyA sequence immediately downstream of the endogenous Foxp3 translational stop codon but upstream of the endogenous polyA signal. This also placed a loxP-flanked PGKneo cassette into intron 9. This vector was electroporated into 129X1Sv/J-derived SCC10 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and chimeric males were mated with C57BL/6 females. The PGKneo cassette was removed by mating founder males with Cre-deleter female mice on a C57BL/6 congenic background. The resulting mice (now harboring this Foxp3EGFP allele) were backcrossed to C57BL/6NCrl for 2 generations by Dr. Talal Chatila (UCLA) and then sent to Dr. Timothy Ley (Washington University). There, mice were further backcrossed 3 generations to C57BL/6J using a genomic screening (speed congenics) approach. The resulting mice (with 98-99% identity to C57BL/6J) were sent back to Dr. Chatila at UCLA prior to arrival at The Jackson Laboratory.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Foxp3tm2Tch | ||
|---|---|---|---|
| Allele Name | targeted mutation 2, Talal A Chatila | ||
| Common Name(s) | Foxp3EGFP; Foxp3tm2(EGFP)Tch; | ||
| Mutation Made By | Talal Chatila, David Geffen School of Medicine, UCLA | ||
| Strain of Origin | 129X1/SvJ | ||
| ES Cell Line Name | Other (see notes) | ||
| ES Cell Line Strain | 129 | ||
| Site of Expression | EGFP is expressed in the Foxp3+ T cell population. Some EGFP expression is noted in a small population of CD8+ thymocytes. | ||
| Gene Symbol and Name | Foxp3, forkhead box P3 | ||
| Chromosome | X | ||
| Gene Common Name(s) | AIID; DIETER; IPEX; JM2; MGC141961; MGC141963; PIDX; RGD1562112; XPID; scurfin; scurfy; sf; | ||
| General Note | ES cell line = SSC10 | ||
| Molecular Note | An IRES-EGFP-SV40 poly A sequence was inserted immediately downstream of the endogenous Foxp3 translational stop codon, but upstream of the endogenous polyA signal, generating a bicistronic locus encoding both Foxp3 and EGFP. Expression of EGFP is restricted to the T cell lineage, primarily to the CD4+ T cell population. [MGI Ref ID J:120713] | ||
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for JAX® GEMM® Strains | ||
Colony Maintenance
| Breeding & Husbandry | This mutant allele is located on the X chromosome. When maintaining a live colony, homozygous females are bred with hemizygous males. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (145 strains)
006769 C.Cg-Foxp3tm2Tch/J
004088 B6.Cg-Foxp3sf/J 006775 NOD.Cg-Foxp3sf/DoiJ
Additional Web Information
Congenic Nomenclature
Fluorescent Proteins/lacZ Systems
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
Immunology and Inflammation Research
Immunodeficiency (T cell deficiency)
Research Tools
Fluorescent Proteins
Genetics Research (Tissue/Cell Markers: T cell specific surface marker)
Immunology and Inflammation Research (T cell specific surface marker)
References
Selected Reference(s)
Additional ReferencesHaribhai D; Lin W; Relland LM; Truong N; Williams CB; Chatila TA. 2007. Regulatory T cells dynamically control the primary immune response to foreign antigen. J Immunol 178(5):2961-72. [PubMed: 17312141] [MGI Ref ID J:120713]
Lin W; Haribhai D; Relland LM; Truong N; Carlson MR; Williams CB; Chatila TA. 2007. Regulatory T cell development in the absence of functional Foxp3. Nat Immunol 8(4):359-68. [PubMed: 17273171] [MGI Ref ID J:120636]
Price and Supply Information
| Strain Name: | B6.Cg-Foxp3tm2Tch/J |
| Stock Number: | 006772 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. The shipping destinations are:
- USA, Canada and Mexico
- International
*Pricing for Shipping Destination selected:
USA, Canada and Mexico
| Price(s) in US dollars ($) | Genotype(s) Provided | ||||
|---|---|---|---|---|---|
| Individual Mouse Price | $155.70 | Homozygous for Foxp3tm2Tch | Female | ||
| Individual Mouse Price | $155.70 | Hemizygous for Foxp3tm2Tch | Male | ||
| Pair | $311.40 | Homozygous for Foxp3tm2Tch x Hemizygous for Foxp3tm2Tch | |||
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Histology and Tissue Collection Services are available for all JAX® Mice strains. For more information, please contact Customer Service at orderquest@jax.org or 1-800-422-6423. Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. View Control Pricing Information for JAX® Strains. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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