Strain Name: |
FVB-Tg(Col2a1-cre/ESR1)KA3Smac/J |
|---|---|
Stock Number: |
006774 |
Availability: | Repository- Live |
General Terms and Conditions |
| Genes & Alleles | Col2a1; Esr1; cre; Tg(Col2a1-cre/ESR1)KA3Smac; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Coisogenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Donating Investigator Susan Mackem, National Cancer Institute Generation F14 (20-DEC-07) Strain Description
Mice hemizygous or homozygous for the Col2CreERT transgene are viable and fertile. Mice from this founder line (line K from founder mouse A3) have strong tamoxifen-inducible cre expression directed to cells of the chondrogenic lineage (cartilage), with minimal (<0.1%) cre activity in the absence of tamoxifen. The CreERT protein consists of Cre recombinase fused to a mutant form of the human estrogen receptor; which does not bind its natural ligand (17b-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligand 4-hydroxytamoxifen. Restricted to the cytoplasm, CreERT can only gain access to the nuclear compartment after exposure to tamoxifen. To counteract the mixed estrogen agonist effects of tamoxifen injections, which can result in late fetal abortions in pregnant mice, progesterone may be coadministered. When these Col2CreERT mice are bred with mice containing a loxP-flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the flanked sequences in cartilage cells (both during embryogenesis and postnatally), as well as in other cell types that normally express the endogenous Type II collagen gene.Strain Development
The Col2CreERT transgene was designed with the mouse Col2a-1 (procollagen type II, alpha 1) promoter sequence, beta-globin intron, Cre-ERT fusion gene (Cre recombinase fused to a mutated ligand-binding domain of the human estrogen receptor (ER)), SV40 pA signal, and Col2a-1 intron 1 enhancer. This transgene was injected into FVB/N zygotes. Founder mice determined to have the strongest tamoxifen-inducible Cre recombinase activity with minimal activity in the absence of tamoxifen (founder line K from founder mouse A3) were subsequently bred to FVB/N to generate hemizygotes. Mice were then interbred for more than 10 generations prior to arrival at The Jackson Laboratory.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Tg(Col2a1-cre/ESR1)KA3Smac | ||
|---|---|---|---|
| Allele Name | transgene insertion KA3, Susan Mackem | ||
| Common Name(s) | Col2CreERT; Tg(Col2a1-cre/ESR1)1Smac; | ||
| Mutation Made By | Susan Mackem, National Cancer Institute | ||
| Strain of Origin | FVB/N | ||
| Expressed Gene | Esr1, estrogen receptor 1 (alpha), mouse, laboratory | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Col2a1, collagen, type II, alpha 1, mouse, laboratory | ||
| Molecular Note | This transgene expresses a tamoxifen inducible form of cre recombinase driven by the Col2a1 promoter in cartilage. The cre recombinase coding region is joined to sequence encoding the ligand binding domain of the human estrogen receptor with a glycine toarginine missense mutation at position 521. This substitution enables the domain to bind to tamoxifen while ablating the estradiol binding ability. [MGI Ref ID J:111627] [MGI Ref ID J:35420] | ||
Control Information
| Control | ||
|---|---|---|
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Generic Cre QPCR
Tg(Col2a1-cre/ESR1)KA3Smac
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, hemizygous or homozygous mice may be bred. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying other alleles of Col2a1
003916 B6(Cg)-Col2a1sedc/J 003554 B6;SJL-Tg(Col2a1-cre)1Bhr/J View Strains carrying other alleles of Col2a1 (2 strains)
006403 129S.B6-Tg(KRT14-Esr1/HRAS)1Pkha/J 004744 B6.129P2-Esr1tm1Ksk/J 004682 B6.Cg-Tg(CAG-cre/Esr1)5Amc/J 005657 B6.Cg-Tg(Myh6-cre/Esr1)1Jmk/J 005650 B6129-Tg(Myh6-cre/Esr1)1Jmk/J 006822 FVB-Tg(KRT14-MAP2K1/Esr1)12Pkha/J 004453 STOCK Tg(CAG-cre/Esr1)5Amc/J 008122 STOCK Tg(Ins2-cre/Esr1)1Dam/J 005107 STOCK Tg(KRT14-cre/Esr1)20Efu/J 008119 STOCK Tg(Neurog3-cre/Esr1)1Dam/J
Additional Web Information
Cre-lox or FLP-FRT Systems
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox System (Cre-Recombinase Expression: Germline/Embryonic Expression)
Cre-lox System (Cre-Recombinase Expression: Inducible)
Developmental Biology Research (Cre-lox System)
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Genetics Research (Tissue/Cell Markers: Cre-lox System)
Genetics Research (Tissue/Cell Markers: chondrocytes)
Research Tools
Cre-lox System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
References
Selected Reference(s)
Additional ReferencesNakamura E; Nguyen MT; Mackem S. 2006. Kinetics of tamoxifen-regulated Cre activity in mice using a cartilage-specific CreER(T) to assay temporal activity windows along the proximodistal limb skeleton. Dev Dyn 235(9):2603-2612. [PubMed: 16894608] [MGI Ref ID J:111627]
Price and Supply Information
| Strain Name: | FVB-Tg(Col2a1-cre/ESR1)KA3Smac/J |
| Stock Number: | 006774 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Notice Regarding cre/Esr1 (Tamoxifen Inducible Cre) Strains.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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