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| When these Col2CreERT mice are bred with mice containing a loxP-flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the flanked sequences in cartilage cells (both during embryogenesis and postnatally), as well as in other cell types that normally express the endogenous Type II collagen gene. | |||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Coisogenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Homozygote x Homozygote (Female x Male) 18-APR-08 Species laboratory mouse Generation F14+F3 (24-DEC-08) Donating Investigator Susan Mackem, National Cancer Institute Description
Mice hemizygous or homozygous for the Col2CreERT transgene are viable and fertile. Mice from this founder line (line K from founder mouse A3) have strong tamoxifen-inducible cre expression directed to cells of the chondrogenic lineage (cartilage), with minimal (<0.1%) cre activity in the absence of tamoxifen. The CreERT protein consists of Cre recombinase fused to a mutant form of the human estrogen receptor; which does not bind its natural ligand (17β-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligand 4-hydroxytamoxifen. Restricted to the cytoplasm, CreERT can only gain access to the nuclear compartment after exposure to tamoxifen. To counteract the mixed estrogen agonist effects of tamoxifen injections, which can result in late fetal abortions in pregnant mice, progesterone may be coadministered. When these Col2CreERT mice are bred with mice containing a loxP-flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the flanked sequences in cartilage cells (both during embryogenesis and postnatally), as well as in other cell types that normally express the endogenous Type II collagen gene.Development
The Col2CreERT transgene was designed with the mouse Col2a-1 (procollagen type II, alpha 1) promoter sequence, beta-globin intron, Cre-ERT fusion gene (Cre recombinase fused to a mutated ligand-binding domain of the human estrogen receptor (ER)), SV40 pA signal, and Col2a-1 intron 1 enhancer. This transgene was injected into FVB/N zygotes. Founder mice determined to have the strongest tamoxifen-inducible Cre recombinase activity with minimal activity in the absence of tamoxifen (founder line K from founder mouse A3) were subsequently bred to FVB/N to generate hemizygotes. Mice were then interbred for more than 10 generations prior to arrival at The Jackson Laboratory.
Control Information
| Control | ||
|---|---|---|
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Col2a1
003916 B6(Cg)-Col2a1sedc/J 003554 B6;SJL-Tg(Col2a1-cre)1Bhr/J View Strains carrying other alleles of Col2a1 (2 strains)
006403 129S.B6-Tg(KRT14-Esr1/HRAS)1Pkha/J 004744 B6.129P2-Esr1tm1Ksk/J 004682 B6.Cg-Tg(CAG-cre/Esr1)5Amc/J 005657 B6.Cg-Tg(Myh6-cre/Esr1)1Jmk/J 005650 B6;129-Tg(Myh6-cre/Esr1)1Jmk/J 006822 FVB-Tg(KRT14-MAP2K1/Esr1)12Pkha/J 008464 STOCK Foxa2tm2.1(cre/Esr1)Moon/J 004453 STOCK Tg(CAG-cre/Esr1)5Amc/J 008122 STOCK Tg(Ins2-cre/Esr1)1Dam/J 005107 STOCK Tg(KRT14-cre/Esr1)20Efu/J 008119 STOCK Tg(Neurog3-cre/Esr1)1Dam/J
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
assigned by genotype
Tg(Col2a1-cre/ESR1)KA3Smac/0
involves: FVB/N
- normal phenotype
- no abnormal phenotype detected (MGI Ref ID J:111627)
- mice are viable and fertile
Tg(Col2a1-cre/ESR1)KA3Smac/Tg(Col2a1-cre/ESR1)KA3Smac
involves: FVB/N
- normal phenotype
- no abnormal phenotype detected (MGI Ref ID J:111627)
- mice are viable and fertile
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox System
Cre Recombinase Expression: Germline/Embryonic Expression
Cre Recombinase Expression: Inducible
Developmental Biology Research
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Tissue/Cell Markers: Cre-lox System
Tissue/Cell Markers: chondrocytes
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Col2a1-cre/ESR1)KA3Smac | ||
|---|---|---|---|
| Allele Name | transgene insertion KA3, Susan Mackem | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | Col2CreERT; Tg(Col2a1-cre/ESR1)1Smac; | ||
| Mutation Made By | Susan Mackem, National Cancer Institute | ||
| Strain of Origin | FVB/N | ||
| Site of Expression | tamoxifen-inducible cre expression is directed to cells of the chondrogenic lineage (cartilage) during embryogenesis and postnatally | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Expressed Gene | Esr1, estrogen receptor 1 (alpha), mouse, laboratory | ||
| Promoter | Col2a1, collagen, type II, alpha 1, mouse, laboratory | ||
| Driver Note | Col2a1 | ||
| Inducible Note | induced by tamoxifen | ||
| Molecular Note | This transgene expresses a tamoxifen inducible form of cre recombinase driven by the Col2a1 promoter in cartilage. The cre recombinase coding region is joined to sequence encoding the ligand binding domain of the human estrogen receptor with a glycine toarginine missense mutation at position 521. This substitution enables the domain to bind to tamoxifen while ablating the estradiol binding ability. [MGI Ref ID J:111627] [MGI Ref ID J:35420] | ||
| Gene Symbol and Name | Tg(Col2a1-cre/ESR1)KA3Smac, transgene insertion KA3, Susan Mackem | ||
| Chromosome | UN | ||
| Gene Common Name(s) | Col2CreERT; Tg(Col2a1-cre/ESR1)1Smac; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Cre Quantitative PCR, QPCR
Generic Cre, Standard PCR
Tg(Col2a1-cre/ESR1)KA3Smac, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Nakamura E; Nguyen MT; Mackem S. 2006. Kinetics of tamoxifen-regulated Cre activity in mice using a cartilage-specific CreER(T) to assay temporal activity windows along the proximodistal limb skeleton. Dev Dyn 235(9):2603-2612. [PubMed: 16894608] [MGI Ref ID J:111627]
Additional References
Tg(Col2a1-cre/ESR1)KA3Smac relatedHou B; Kolpakova-Hart E; Fukai N; Wu K; Olsen BR. 2009. The polycystic kidney disease 1 (Pkd1) gene is required for the responses of osteochondroprogenitor cells to midpalatal suture expansion in mice. Bone 44(6):1121-33. [PubMed: 19264154] [MGI Ref ID J:150386]
Koyama E; Young B; Nagayama M; Shibukawa Y; Enomoto-Iwamoto M; Iwamoto M; Maeda Y; Lanske B; Song B; Serra R; Pacifici M. 2007. Conditional Kif3a ablation causes abnormal hedgehog signaling topography, growth plate dysfunction, and excessive bone and cartilage formation during mouse skeletogenesis. Development 134(11):2159-69. [PubMed: 17507416] [MGI Ref ID J:122610]
Maeda Y; Nakamura E; Nguyen MT; Suva LJ; Swain FL; Razzaque MS; Mackem S; Lanske B. 2007. Indian Hedgehog produced by postnatal chondrocytes is essential for maintaining a growth plate and trabecular bone. Proc Natl Acad Sci U S A 104(15):6382-7. [PubMed: 17409191] [MGI Ref ID J:120822]
Mak KK; Kronenberg HM; Chuang PT; Mackem S; Yang Y. 2008. Indian hedgehog signals independently of PTHrP to promote chondrocyte hypertrophy. Development 135(11):1947-56. [PubMed: 18434416] [MGI Ref ID J:134987]
Tsumaki N; Kimura T; Matsui Y; Nakata K; Ochi T. 1996. Separable cis-regulatory elements that contribute to tissue- and site-specific alpha 2(XI) collagen gene expression in the embryonic mouse cartilage. J Cell Biol 134(6):1573-82. [PubMed: 8830784] [MGI Ref ID J:35420]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, hemizygous or homozygous mice may be bred. Mating System Homozygote x Homozygote (Female x Male) 18-APR-08 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $209.90 Female or Male Homozygous for Tg(Col2a1-cre/ESR1)KA3Smac
Pairs /Price (US dollars $) Pair Genotype $419.80 Homozygous for Tg(Col2a1-cre/ESR1)KA3Smac x Homozygous for Tg(Col2a1-cre/ESR1)KA3Smac
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $272.90 Female or Male Homozygous for Tg(Col2a1-cre/ESR1)KA3Smac
Pairs /Price (US dollars $) Pair Genotype $545.80 Homozygous for Tg(Col2a1-cre/ESR1)KA3Smac x Homozygous for Tg(Col2a1-cre/ESR1)KA3Smac
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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