Strain Name: |
NOD/ShiLt-Tg(Ins2-Cd274)2Mdos/MdosJ |
|---|---|
Stock Number: |
006777 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Former Name |
NOD-Tg(Ins2-Cd274)2Mdos/MdosJ (Changed: 07-MAR-07
) |
| Strain Common Names | NOD.RIP-B7H1; NOD.RIP-PDL1; |
| Genes & Alleles | Cd274; Ins2; Tg(Ins2-Cd274)2Mdos; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Coisogenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Species laboratory mouse Background Strain NOD/ShiLtJ Donating Investigator H. Dosch, University of Toronto H2 Haplotype g7 Appearance
albino, pink eyed
Related Genotype: A/A Tyrc/TyrcStrain Description
Ins2-Cd274 (Rip-B7H1) transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. The donating investigator indicates transgenic mice over express Cd274 in pancreatic beta cells and reports accelerated diabetes in Ins2-Cd274 transgenic mice compared to wildtype cohorts.This strain is useful to study the role of Cd274 in autoimmunity and peripheral tolerance.
Strain Development
A member of the CD80/86 (B7) gene family, CD274, commonly called B7H1 or PD-L1, is normally expressed in antigen presenting cells and non-lymphoid tissues such as heart and lung. This transgenic strain carries a CD274 (B7H1) cDNA fused to thrat insulin promoter (Ins2) injected into NOD/ShiLt fertilized embryos. Progeny of founder 2 were mated to NOD/ShiLt. In 2006, the T1DR received hemizygous mice that were mated to NOD/ShiLtJ (Stock No. 001976) prior to sibling mating.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Tg(Ins2-Cd274)2Mdos | ||
|---|---|---|---|
| Allele Name | transgene insertion 2, H Michael Dosch | ||
| Common Name(s) | RIP-B7H1; RIP-PDL1L; | ||
| Mutation Made By | H. Dosch, University of Toronto | ||
| Strain of Origin | NOD/ShiLt | ||
| Expressed Gene | Cd274, CD274 antigen, mouse, laboratory | ||
| Promoter | Ins2, insulin 2, rat | ||
| Molecular Note | This transgene contains cDNA encoding human CD274 (B7H1) fused to the rat insulin 2 promoter. Transgenic mice express CD274 in pancreatic beta cells. [MGI Ref ID J:118934] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001976 NOD/ShiLtJ | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Tg(Ins2-Cd274)2Mdos
Related Strains
Strains carrying other alleles of Ins2
View Strains carrying other alleles of Ins2 (42 strains)
Research Applications
This mouse can be used to support research in many areas including:
Diabetes and Obesity Research
Type 1 Diabetes (IDDM)
Type 1 Diabetes (IDDM) Analysis Strains (NOD Transgenics)
Immunology and Inflammation Research
Autoimmunity (Type 1 Diabetes)
CD Antigens, Antigen Receptors, and Histocompatibility Markers
References
Additional ReferencesPrice and Supply Information
| Strain Name: | NOD/ShiLt-Tg(Ins2-Cd274)2Mdos/MdosJ |
| Stock Number: | 006777 |
Price Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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