Strain Name:

NOD/ShiLt-Tg(GFAP-Cd80)9Mdos/MdosJ

Stock Number:

006778

Availability:

Cryopreserved - Ready for recovery

Common Names: NOD.Gfap-B7-1;    

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names NOD/ShiLt-Tg(Gfap-Cd80)9Mdos/MdosJ    (Changed: 18-MAR-08 )
Type Coisogenic; Mutant Stock; Mutant Strain; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
H2 Haplotypeg7
Generation?+N1F2pN1
 
Donating Investigator H. Dosch,   University of Toronto

Appearance
albino, pink eyed
Related Genotype: A/A Tyrc/Tyrc

Description
Gfap-Cd80 (GFAP-B7-1) transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. The donating investigator reports that transgenic mice are protected from diabetes development.

Development
This transgenic strain carries a T cell co-stimulatory molecule, Cd80 (B7-1), cDNA fused to the glial fibrillary acidic protein (Gfap) promoter. The transgenic construct was injected into NOD/ShiLt fertilized embryos, and progeny of founder 9 were mated to NOD/ShiLt. In 2006, the T1DR received hemizygous mice that were mated to NOD/ShiLtJ (Stock No. 001976) prior to sibling mating.

Control Information

  Control
   Noncarrier
   001976 NOD/ShiLtJ
 
  Considerations for Choosing Controls

Related Strains

NOD Strains
001976   NOD/ShiLtJ
View NOD Strains     (1 strain)

View Strains carrying other alleles of Cd80     (5 strains)

View Strains carrying other alleles of GFAP     (12 strains)

Phenotype

Phenotype Information

View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Diabetes and Obesity Research
Type 1 Diabetes (IDDM)
Type 1 Diabetes (IDDM) Analysis Strains
      NOD Transgenics

Immunology and Inflammation Research
Autoimmunity
      Type 1 Diabetes
CD Antigens, Antigen Receptors, and Histocompatibility Markers

Genes & Alleles

Gene & Allele Information

 
Allele Symbol Tg(GFAP-CD80)9Mdos
Allele Name transgene insertion 9, H Michael Dosch
Allele Type Transgenic (random, expressed)
Common Name(s) GFAP-B7-1Tg line 9; GFAP-B71; Tg(Gfap-CD80)9Mdos;
Strain of OriginNOD/ShiLt
Expressed Gene Cd80, CD80 antigen, mouse, laboratory
Promoter GFAP, glial fibrillary acidic protein, human
Molecular Note This transgene contains cDNA encoding CD80 under control of the glial fibrillary acidic protein promoter. [MGI Ref ID J:118933]
 
 

Genotyping

Genotyping Information

Genotyping Protocols

Tg(GFAP-CD80)9Mdos, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References

Additional References

Tg(GFAP-CD80)9Mdos related

Dosch HM. 2007. Transgenic expression of CD80 in the central nervous system provides protection against diabetes development in Non-Obese Diabetic mice MGI Direct Data Submission :.  [MGI Ref ID J:118933]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Pricing for USA, Canada and Mexico shipping destinations View International pricing
Price (US dollars $)
Cryorecovery Fee $1900.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Additional Supply Details

Pricing for International shipping destinations View USA Canada and Mexico pricing
Price (US dollars $)
Cryorecovery Fee $2470.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Additional Supply Details

Supply Details

Standard SupplyCryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
  • Cryorecovery - Standard.
    We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

  • This strain is included in the Type 1 Diabetes Repository collection.
  • Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

  Control
   Noncarrier
   001976 NOD/ShiLtJ
 
  Considerations for Choosing Controls
  USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
  International - Control Pricing Information for Genetically Engineered Mutant Strains.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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