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- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names STOCK Tg(Ins1-ECFP)24Hara/J (Changed: 13-JAN-10 ) Type Mutant Stock; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation ?+N1F1pN1
Generation DefinitionsDonating Investigator Manami Hara, University of Chicago Description
Mice hemizygous for this "MIP-CFP" transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express the Cerulean variant of Enhanced Cyan Fluorescent Protein under the control of mouse insulin 1 promoter (MIP). Fluorescence is detected in tissues where insulin I is normally expressed; fluorescent protein expression in pancreatic beta-cells is evident from embryonic day (E)13.5 through adult. The fluorescence expression pattern observed in this strain is similar to the patterns seen in other stocks that express fluorescent proteins from the MIP promoter (see Stock No. 006864 and Stock No. 006866). The human growth hormone sequence in the transgene is designed to enhance expression of the fluorescent protein. Although the expression of this sequence in this strain has not been characterized, the sequence is not expressed in a similarly constructed transgenic strain (see Stock No. 006864). This mutant mouse strain may be useful in studies of diabetes and pancreatic beta islet cell biology.Development
A transgenic construct containing an Enhanced Cyan Fluorescent Protein (Cerulean) gene and 2.1kb of sequence from the human growth hormone gene (to confer high expression of the ECFP) under the control of the mouse insulin 1 promoter was injected into fertilized CD1 mouse eggs. Founder line 24 was subsequently established. Upon arrival at The Jackson Laboratory, transgenic mice were bred with C57BL/6J to establish this colony on a mixed genetic background.
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (357 strains)
004218 B6.129(ICR)-Tg(CAG-ECFP)CK6Nagy/J 008513 B6.129P2-Gt(ROSA)26Sortm1(Trpv1,ECFP)Mde/J 007901 B6.Cg-Tg(Thy1-Brainbow1.0)HLich/J 007911 B6.Cg-Tg(Thy1-Brainbow1.1)MLich/J 007921 B6.Cg-Tg(Thy1-Brainbow2.1)RLich/J 003710 B6.Cg-Tg(Thy1-CFP)23Jrs/J 014131 B6.Cg-Tg(Thy1-CFP)IJrs/GfngJ 007940 B6.Cg-Tg(Thy1-CFP/COX8A)C1Lich/J 007967 B6.Cg-Tg(Thy1-CFP/COX8A)S2Lich/J 013161 B6.Cg-Tg(Thy1-Clomeleon)1Gjau/J 007910 B6;CBA-Tg(Thy1-Brainbow1.0)LLich/J 008004 B6;SJL-Tg(Thy1-ECFP/VAMP2)1Sud/J 007856 B6SJL-Tg(Thy1-Syt1/ECFP)1Sud/J 018671 D2.Cg-Tg(Thy1-CFP)23Jrs/SjJ 018067 FVB-Tg(Prism)1849Htz/J 018068 FVB-Tg(Prism)1989Htz/J 007879 STOCK Stx1atm2Sud/J 003773 STOCK Tg(CAG-ECFP)CK6Nagy/J 016922 STOCK Tg(Myh7-CFP)1Jrs/J 013162 STOCK Tg(Thy1-Clomeleon)12Gjau/J 013163 STOCK Tg(Thy1-Clomeleon)13Gjau/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
Tg(Ins1-Cerulean)24Hara/0
involves: CD-1
- normal phenotype
- no abnormal phenotype detected
- no phenotypic abnormalities reported (MGI Ref ID J:120245)
This mouse can be used to support research in many areas including:
Research Tools
Diabetes and Obesity Research
Fluorescent Proteins
Genetics Research
Tissue/Cell Markers
Tissue/Cell Markers: pancreatic beta cells
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Ins1-Cerulean)24Hara | ||
|---|---|---|---|
| Allele Name | transgene insertion 24, Manami Hara | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | MIP-CFP (line 24); Tg(Ins1-ECFP)24Hara; | ||
| Mutation Made By | Manami Hara, University of Chicago | ||
| Strain of Origin | CD-1 | ||
| Site of Expression | ECFP expression is detected in tissues where insulin I is normally expressed. ECFP expression in pancreatic beta-cells is evident from embryonic day (E)13.5 through adult. | ||
| Expressed Gene | CFP, Cyan Fluorescent Protein, jellyfish | ||
| Promoter | Ins1, insulin I, mouse, laboratory | ||
| Molecular Note | The transgenic construct consists of an Enhanced Cyan Fluorescent Protein (Cerulean) gene and 2.1kb of sequence from the human growth hormone (GH1) gene (to confer high expression of the ECFP) under the control of the mouse insulin 1 promoter. Founder line 24 was subsequently established. Expression of ECFP was detected specifically in beta cells of pancreatic islets. [MGI Ref ID J:120245] [MGI Ref ID J:99450] | ||
| Gene Symbol and Name | Tg(Ins1-Cerulean)24Hara, transgene insertion 24, Manami Hara | ||
| Chromosome | UN | ||
| Gene Common Name(s) | MIP-CFP (line 24); | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(Ins1-Cerulean)24Hara, QPCR
Tg(Ins1-Cerulean)24Hara, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Hara M; Dizon RF; Glick BS; Lee CS; Kaestner KH; Piston DW; Bindokas VP. 2006. Imaging pancreatic beta-cells in the intact pancreas. Am J Physiol Endocrinol Metab 290(5):E1041-7. [PubMed: 16368785] [MGI Ref ID J:120245]
Additional References
Tg(Ins1-Cerulean)24Hara relatedHara M; Wang X; Kawamura T; Bindokas VP; Dizon RF; Alcoser SY; Magnuson MA; Bell GI. 2003. Transgenic mice with green fluorescent protein-labeled pancreatic beta -cells. Am J Physiol Endocrinol Metab 284(1):E177-83. [PubMed: 12388130] [MGI Ref ID J:99450]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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