| |||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
Description
Strain Information
Type Congenic; Mutant Strain; Targeted Mutation; Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Generation N11+F2 (08-JAN-08) Donating Investigator Klaus Rajewsky, Immune Disease Institute (formerly CBRI) Description
Homozygous mutant mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Homozygotes have a deficiency in the B-1 subset of B-lymphocytes along with a concomitant reduction in serum IgM. Their ability to respond to T-cell-dependent antigens is severely impaired, and they fail to form splenic germinal centers.In addition to disrupting the targeted gene, the targeting construct also introduced a cre cassette into exon 2 of the targeted gene, effectively placing cre expression under the control of the endogenous promoter. The Cd19 promoter specifically directs cre expression at the earliest stages and throughout B-lymphocyte development and differentiation. Although homozygous mutant mice are Cd19-deficient, heterozygous mice are phenotypically normal, and can be used for specific deletion of loxP-flanked (floxed) targets in B-lymphocytes.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Development
A targeting vector containing Cre recombinase, the rabbit beta-globin intron /poly A signal sequence, and an FRT-flanked neomycin resistance gene, was used to disrupt exon 2 of the Cd19 gene and to express cre under the regulation of the endogenous promoter. Herpes simplex virus thymidine kinase gene was placed 3' of the Cd19 sequence to allow for the selection against random integration. The construct was transfected into 129P2/OlaHsd-derived E14-1 embryonic stem cells. Correctly targeted ES cells were injected into 129/Sv blastocysts. The resulting chimeric animals were backcrossed to BALB/c IgHb congenics for a number of generations and then backcrossed to C57BL/6 for 10 generations.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying Cd19tm1(cre)Cgn allele
004126 C.Cg-Cd19tm1(cre)Cgn Ighb/J View Strains carrying Cd19tm1(cre)Cgn (1 strain)
Additional Web Information
Congenic Nomenclature
Cre-lox Systems
Phenotype
Phenotype Information
Mammalian Phenotype Terms assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Cd19tm1(cre)Cgn/Cd19tm1(cre)Cgn
involves: 129P2/OlaHsd
- hematopoietic system phenotype
- abnormal B cell differentiation (MGI Ref ID J:27463)
- absent spleen germinal center (MGI Ref ID J:27463)
- failure of germinal center formation
- decreased B-1 B cell number (MGI Ref ID J:27463)
- reduced numbers of B1 cells (cd23-, IgM hi, IgD lo)
- immune system phenotype
- abnormal B cell differentiation (MGI Ref ID J:27463)
- abnormal B cell physiology (MGI Ref ID J:27463)
- impaired Cd19-/- B cell response to T cell dependent antigens
- abnormal immunoglobulin level (MGI Ref ID J:27463)
- failure of affinity maturation of serum antibodies
- decreased IgM level (MGI Ref ID J:27463)
- reduction in serum IgM
- absent spleen germinal center (MGI Ref ID J:27463)
- failure of germinal center formation
- decreased B-1 B cell number (MGI Ref ID J:27463)
- reduced numbers of B1 cells (cd23-, IgM hi, IgD lo)
Research Applications
This mouse can be used to support research in many areas including:
cre relatedHematological Research
Hematopoietic Defects
Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Immunodeficiency (B cell defects)
Research Tools
Cre-lox System (Cre Recombinase Expression)
Developmental Biology Research (Cre-lox System)
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Cd19tm1(cre)Cgn relatedResearch Tools
Cre-lox System
Hematological Research
Hematopoietic Defects
Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Immunodeficiency (B cell defects)
Research Tools
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Cd19tm1(cre)Cgn | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, University of Cologne | ||
| Common Name(s) | CD19-; CD19-Cre; CD19-KO; Cd19Cre; Cd19cre; | ||
| Mutation Made By | Robert Rickert, The Burnham Institute | ||
| Strain of Origin | 129P2/OlaHsd | ||
| ES Cell Line Name | E14.1 | ||
| ES Cell Line Strain | 129P2/OlaHsd | ||
| Site of Expression | B cells | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Gene Symbol and Name | Cd19, CD19 antigen | ||
| Chromosome | 7 | ||
| Gene Common Name(s) | AW495831; B4; MGC12802; expressed sequence AW495831; | ||
| Molecular Note | In frame insertion of a cre recombinase gene into the first coding exon followed by an frt-flanked neomycin cassette. A frameshift mutation was also introduced into exon 5. This allele expresses cre recombinase specifically in B lineage cells throughout development. [MGI Ref ID J:27463] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Cd19tm1(cre)Cgn, , vers. 1
Cd19tm1(cre)Cgn, SEP PCR, vers. 2
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Rickert RC; Roes J; Rajewsky K. 1997. B lymphocyte-specific, Cre-mediated mutagenesis in mice. Nucleic Acids Res 25(6):1317-8. [PubMed: 9092650] [MGI Ref ID J:67676]
Additional References
Cd19tm1(cre)Cgn related
Anzelon AN; Wu H; Rickert RC. 2003. Pten inactivation alters peripheral B lymphocyte fate and reconstitutes CD19 function. Nat Immunol 4(3):287-94. [PubMed: 12563260] [MGI Ref ID J:83213]Arana E; Vehlow A; Harwood NE; Vigorito E; Henderson R; Turner M; Tybulewicz VL; Batista FD. 2008. Activation of the small GTPase Rac2 via the B cell receptor regulates B cell adhesion and immunological-synapse formation. Immunity 28(1):88-99. [PubMed: 18191593] [MGI Ref ID J:131150]
Beisner DR; Ch'en IL; Kolla RV; Hoffmann A; Hedrick SM. 2005. Cutting edge: innate immunity conferred by B cells is regulated by caspase-8. J Immunol 175(6):3469-73. [PubMed: 16148088] [MGI Ref ID J:116713]
Brockschnieder D; Lappe-Siefke C; Goebbels S; Boesl MR; Nave KA; Riethmacher D. 2004. Cell depletion due to diphtheria toxin fragment A after Cre-mediated recombination. Mol Cell Biol 24(17):7636-42. [PubMed: 15314171] [MGI Ref ID J:92789]
Buch T; Heppner FL; Tertilt C; Heinen TJ; Kremer M; Wunderlich FT; Jung S; Waisman A. 2005. A Cre-inducible diphtheria toxin receptor mediates cell lineage ablation after toxin administration. Nat Methods 2(6):419-26. [PubMed: 15908920] [MGI Ref ID J:131076]
Cazac BB; Roes J. 2000. TGF-beta receptor controls B cell responsiveness and induction of IgA in vivo. Immunity 13(4):443-51. [PubMed: 11070163] [MGI Ref ID J:90558]
Cinamon G; Zachariah MA; Lam OM; Foss FW Jr; Cyster JG. 2008. Follicular shuttling of marginal zone B cells facilitates antigen transport. Nat Immunol 9(1):54-62. [PubMed: 18037889] [MGI Ref ID J:130200]
Cobaleda C; Jochum W; Busslinger M. 2007. Conversion of mature B cells into T cells by dedifferentiation to uncommitted progenitors. Nature 449(7161):473-7. [PubMed: 17851532] [MGI Ref ID J:126362]
Coles MC; Veiga-Fernandes H; Foster KE; Norton T; Pagakis SN; Seddon B; Kioussis D. 2006. Role of T and NK cells and IL7/IL7r interactions during neonatal maturation of lymph nodes. Proc Natl Acad Sci U S A 103(36):13457-62. [PubMed: 16938836] [MGI Ref ID J:112903]
Del Nagro CJ; Kolla RV; Rickert RC. 2005. A critical role for complement C3d and the B cell coreceptor (CD19/CD21) complex in the initiation of inflammatory arthritis. J Immunol 175(8):5379-89. [PubMed: 16210644] [MGI Ref ID J:119107]
Delogu A; Schebesta A; Sun Q; Aschenbrenner K; Perlot T; Busslinger M. 2006. Gene repression by Pax5 in B cells is essential for blood cell homeostasis and is reversed in plasma cells. Immunity 24(3):269-81. [PubMed: 16546096] [MGI Ref ID J:113322]
Depoil D; Fleire S; Treanor BL; Weber M; Harwood NE; Marchbank KL; Tybulewicz VL; Batista FD. 2008. CD19 is essential for B cell activation by promoting B cell receptor-antigen microcluster formation in response to membrane-bound ligand. Nat Immunol 9(1):63-72. [PubMed: 18059271] [MGI Ref ID J:130478]
Diamant E; Keren Z; Melamed D. 2005. CD19 regulates positive selection and maturation in B lymphopoiesis: lack of CD19 imposes developmental arrest of immature B cells and consequential stimulation of receptor editing. Blood 105(8):3247-54. [PubMed: 15644414] [MGI Ref ID J:98448]
Donahue AC; Hess KL; Ng KL; Fruman DA. 2004. Altered splenic B cell subset development in mice lacking phosphoinositide 3-kinase p85{alpha} Int Immunol 16(12):1789-1798. [PubMed: 15520044] [MGI Ref ID J:94022]
Ellinghaus U; Rupec RA; Pabst O; Ignatius R; Forster R; Dorken B; Jundt F. 2008. IkappaBalpha is required for marginal zone B cell lineage development. Eur J Immunol 38(8):2096-105. [PubMed: 18604869] [MGI Ref ID J:138198]
Fehr T; Rickert RC; Odermatt B; Roes J; Rajewsky K; Hengartner H; Zinkernagel RM. 1998. Antiviral protection and germinal center formation, but impaired B cell memory in the absence of CD19. J Exp Med 188(1):145-55. [PubMed: 9653091] [MGI Ref ID J:110754]
Fleige A; Alberti S; Grobe L; Frischmann U; Geffers R; Muller W; Nordheim A; Schippers A. 2007. Serum response factor contributes selectively to lymphocyte development. J Biol Chem 282(33):24320-8. [PubMed: 17591768] [MGI Ref ID J:124767]
Fornek JL; Tygrett LT; Waldschmidt TJ; Poli V; Rickert RC; Kansas GS. 2006. Critical role for Stat3 in T-dependent terminal differentiation of IgG B cells. Blood 107(3):1085-91. [PubMed: 16223771] [MGI Ref ID J:127212]
Garbi N; Tanaka S; Momburg F; Hammerling GJ. 2006. Impaired assembly of the major histocompatibility complex class I peptide-loading complex in mice deficient in the oxidoreductase ERp57. Nat Immunol 7(1):93-102. [PubMed: 16311600] [MGI Ref ID J:112606]
Gardam S; Sierro F; Basten A; Mackay F; Brink R. 2008. TRAF2 and TRAF3 signal adapters act cooperatively to control the maturation and survival signals delivered to B cells by the BAFF receptor. Immunity 28(3):391-401. [PubMed: 18313334] [MGI Ref ID J:132877]
Gardby E; Chen XJ; Lycke NY. 2001. Impaired CD40-signalling in CD19-deficient mice selectively affects Th2-dependent isotype switching. Scand J Immunol 53(1):13-23. [PubMed: 11169202] [MGI Ref ID J:103806]
Gardby E; Lycke NY. 2000. CD19-deficient mice exhibit poor responsiveness to oral immunization despite evidence of unaltered total IgA levels, germinal centers and IgA-isotype switching in Peyer's patches Eur J Immunol 30(7):1861-71. [PubMed: 10940875] [MGI Ref ID J:63502]
Grivennikov SI; Tumanov AV; Liepinsh DJ; Kruglov AA; Marakusha BI; Shakhov AN; Murakami T; Drutskaya LN; Forster I; Clausen BE; Tessarollo L; Ryffel B; Kuprash DV; Nedospasov SA. 2005. Distinct and nonredundant in vivo functions of TNF produced by t cells and macrophages/neutrophils: protective and deleterious effects. Immunity 22(1):93-104. [PubMed: 15664162] [MGI Ref ID J:95684]
Habib T; Park H; Tsang M; de Alboran IM; Nicks A; Wilson L; Knoepfler PS; Andrews S; Rawlings DJ; Eisenman RN; Iritani BM. 2007. Myc stimulates B lymphocyte differentiation and amplifies calcium signaling. J Cell Biol 179(4):717-31. [PubMed: 17998397] [MGI Ref ID J:135554]
Han JH; Akira S; Calame K; Beutler B; Selsing E; Imanishi-Kari T. 2007. Class switch recombination and somatic hypermutation in early mouse B Cells are mediated by B Cell and toll-like receptors. Immunity 27(1):64-75. [PubMed: 17658280] [MGI Ref ID J:123630]
Hao Z; Hampel B; Yagita H; Rajewsky K. 2004. T cell-specific ablation of Fas leads to Fas ligand-mediated lymphocyte depletion and inflammatory pulmonary fibrosis. J Exp Med 199(10):1355-65. [PubMed: 15148335] [MGI Ref ID J:114948]
Hayashi K; Yamamoto M; Nojima T; Goitsuka R; Kitamura D. 2003. Distinct signaling requirements for Dmu selection, IgH allelic exclusion, pre-B cell transition, and tumor suppression in B cell progenitors. Immunity 18(6):825-36. [PubMed: 12818163] [MGI Ref ID J:84040]
Hobeika E; Thiemann S; Storch B; Jumaa H; Nielsen PJ; Pelanda R; Reth M. 2006. Testing gene function early in the B cell lineage in mb1-cre mice. Proc Natl Acad Sci U S A 103(37):13789-94. [PubMed: 16940357] [MGI Ref ID J:113645]
Horcher M; Souabni A; Busslinger M. 2001. Pax5/BSAP maintains the identity of B cells in late B lymphopoiesis. Immunity 14(6):779-90. [PubMed: 11420047] [MGI Ref ID J:75504]
Inui S; Maeda K; Hua DR; Yamashita T; Yamamoto H; Miyamoto E; Aizawa S; Sakaguchi N. 2002. BCR signal through alpha 4 is involved in S6 kinase activation and required for B cell maturation including isotype switching and V region somatic hypermutation. Int Immunol 14(2):177-87. [PubMed: 11809737] [MGI Ref ID J:74619]
Iwasaki H; Somoza C; Shigematsu H; Duprez EA; Iwasaki-Arai J; Mizuno S; Arinobu Y; Geary K; Zhang P; Dayaram T; Fenyus ML; Elf S; Chan S; Kastner P; Huettner CS; Murray R; Tenen DG; Akashi K. 2005. Distinctive and indispensable roles of PU.1 in maintenance of hematopoietic stem cells and their differentiation. Blood 106(5):1590-600. [PubMed: 15914556] [MGI Ref ID J:114150]
Janas ML; Hodson D; Stamataki Z; Hill S; Welch K; Gambardella L; Trotman LC; Pandolfi PP; Vigorito E; Turner M. 2008. The effect of deleting p110delta on the phenotype and function of PTEN-deficient B cells. J Immunol 180(2):739-46. [PubMed: 18178811] [MGI Ref ID J:130950]
Jungbeck M; Stopfer P; Bataille F; Nedospasov SA; Mannel DN; Hehlgans T. 2008. Blocking lymphotoxin beta receptor signalling exacerbates acute DSS-induced intestinal inflammation-Opposite functions for surface lymphotoxin expressed by T and B lymphocytes. Mol Immunol 45(1):34-41. [PubMed: 17590442] [MGI Ref ID J:124292]
Junt T; Tumanov AV; Harris N; Heikenwalder M; Zeller N; Kuprash DV; Aguzzi A; Ludewig B; Nedospasov SA; Zinkernagel RM. 2006. Expression of lymphotoxin beta governs immunity at two distinct levels. Eur J Immunol 36(8):2061-75. [PubMed: 16841297] [MGI Ref ID J:116025]
Keren Z; Diamant E; Ostrovsky O; Bengal E; Melamed D. 2004. Modification of ligand-independent B cell receptor tonic signals activates receptor editing in immature B lymphocytes. J Biol Chem 279(14):13418-24. [PubMed: 14668327] [MGI Ref ID J:89118]
Kirstetter P; Anderson K; Porse BT; Jacobsen SE; Nerlov C. 2006. Activation of the canonical Wnt pathway leads to loss of hematopoietic stem cell repopulation and multilineage differentiation block. Nat Immunol 7(10):1048-56. [PubMed: 16951689] [MGI Ref ID J:112660]
Kitaura Y; Jang IK; Wang Y; Han YC; Inazu T; Cadera EJ; Schlissel M; Hardy RR; Gu H. 2007. Control of the B cell-intrinsic tolerance programs by ubiquitin ligases Cbl and Cbl-b. Immunity 26(5):567-78. [PubMed: 17493844] [MGI Ref ID J:123596]
Klein J; Ju W; Heyer J; Wittek B; Haneke T; Knaus P; Kucherlapati R; Bottinger EP; Nitschke L; Kneitz B. 2006. B cell-specific deficiency for Smad2 in vivo leads to defects in TGF-beta-directed IgA switching and changes in B cell fate. J Immunol 176(4):2389-96. [PubMed: 16455997] [MGI Ref ID J:108099]
Knoetig SM; Torrey TA; Naghashfar Z; McCarty T; Morse HC rd. 2002. CD19 signaling pathways play a major role for murine AIDS induction and progression. J Immunol 169(10):5607-14. [PubMed: 12421939] [MGI Ref ID J:80060]
Kontoyiannis D; Boulougouris G; Manoloukos M; Armaka M; Apostolaki M; Pizarro T; Kotlyarov A; Forster I; Flavell R; Gaestel M; Tsichlis P; Cominelli F; Kollias G. 2002. Genetic dissection of the cellular pathways and signaling mechanisms in modeled tumor necrosis factor-induced Crohn's-like inflammatory bowel disease. J Exp Med 196(12):1563-74. [PubMed: 12486099] [MGI Ref ID J:108572]
Kracker S; Bergmann Y; Demuth I; Frappart PO; Hildebrand G; Christine R; Wang ZQ; Sperling K; Digweed M; Radbruch A. 2005. Nibrin functions in Ig class-switch recombination. Proc Natl Acad Sci U S A 102(5):1584-9. [PubMed: 15668383] [MGI Ref ID J:96104]
Kroll J; Shi X; Caprioli A; Liu HH; Waskow C; Lin KM; Miyazaki T; Rodewald HR; Sato TN. 2005. The BTB-kelch protein KLHL6 is involved in B-lymphocyte antigen receptor signaling and germinal center formation. Mol Cell Biol 25(19):8531-40. [PubMed: 16166635] [MGI Ref ID J:101363]
Kuwahara K; Fujimura S; Takahashi Y; Nakagata N; Takemori T; Aizawa S; Sakaguchi N. 2004. Germinal center-associated nuclear protein contributes to affinity maturation of B cell antigen receptor in T cell-dependent responses. Proc Natl Acad Sci U S A 101(4):1010-5. [PubMed: 14715907] [MGI Ref ID J:88276]
Kwon K; Hutter C; Sun Q; Bilic I; Cobaleda C; Malin S; Busslinger M. 2008. Instructive role of the transcription factor E2A in early B lymphopoiesis and germinal center B cell development. Immunity 28(6):751-62. [PubMed: 18538592] [MGI Ref ID J:137719]
Lacy-Hulbert A; Smith AM; Tissire H; Barry M; Crowley D; Bronson RT; Roes JT; Savill JS; Hynes RO. 2007. Ulcerative colitis and autoimmunity induced by loss of myeloid alphav integrins. Proc Natl Acad Sci U S A 104(40):15823-8. [PubMed: 17895374] [MGI Ref ID J:125508]
Le Bon A; Thompson C; Kamphuis E; Durand V; Rossmann C; Kalinke U; Tough DF. 2006. Cutting edge: enhancement of antibody responses through direct stimulation of B and T cells by type I IFN. J Immunol 176(4):2074-8. [PubMed: 16455962] [MGI Ref ID J:129128]
Lemmers B; Salmena L; Bidere N; Su H; Matysiak-Zablocki E; Murakami K; Ohashi PS; Jurisicova A; Lenardo M; Hakem R; Hakem A. 2007. Essential role for caspase-8 in Toll-like receptors and NFkappaB signaling. J Biol Chem 282(10):7416-23. [PubMed: 17213198] [MGI Ref ID J:120896]
Li ZW; Omori SA; Labuda T; Karin M; Rickert RC. 2003. IKK beta is required for peripheral B cell survival and proliferation. J Immunol 170(9):4630-7. [PubMed: 12707341] [MGI Ref ID J:82995]
Liu B; Li Z. 2008. Endoplasmic reticulum HSP90b1 (gp96, grp94) optimizes B-cell function via chaperoning integrin and TLR but not immunoglobulin. Blood 112(4):1223-30. [PubMed: 18509083] [MGI Ref ID J:138420]
Lyubchenko T; dal Porto J; Cambier JC; Holers VM. 2005. Coligation of the B cell receptor with complement receptor type 2 (CR2/CD21) using its natural ligand C3dg: activation without engagement of an inhibitory signaling pathway. J Immunol 174(6):3264-72. [PubMed: 15749857] [MGI Ref ID J:97685]
Nie Y; Waite J; Brewer F; Sunshine MJ; Littman DR; Zou YR. 2004. The role of CXCR4 in maintaining peripheral B cell compartments and humoral immunity. J Exp Med 200(9):1145-56. [PubMed: 15520246] [MGI Ref ID J:94911]
Nurieva RI; Chung Y; Hwang D; Yang XO; Kang HS; Ma L; Wang YH; Watowich SS; Jetten AM; Tian Q; Dong C. 2008. Generation of T follicular helper cells is mediated by interleukin-21 but independent of T helper 1, 2, or 17 cell lineages. Immunity 29(1):138-49. [PubMed: 18599325] [MGI Ref ID J:137869]
Oberdoerffer P; Kanellopoulou C; Heissmeyer V; Paeper C; Borowski C; Aifantis I; Rao A; Rajewsky K. 2005. Efficiency of RNA interference in the mouse hematopoietic system varies between cell types and developmental stages. Mol Cell Biol 25(10):3896-905. [PubMed: 15870264] [MGI Ref ID J:98881]
Ochsenbein AF; Pinschewer DD; Odermatt B; Carroll MC; Hengartner H; Zinkernagel RM. 1999. Protective T cell-independent antiviral antibody responses are dependent on complement. J Exp Med 190(8):1165-74. [PubMed: 10523614] [MGI Ref ID J:115126]
Omori SA; Cato MH; Anzelon-Mills A; Puri KD; Shapiro-Shelef M; Calame K; Rickert RC. 2006. Regulation of class-switch recombination and plasma cell differentiation by phosphatidylinositol 3-kinase signaling. Immunity 25(4):545-57. [PubMed: 17000121] [MGI Ref ID J:114881]
Opferman JT; Letai A; Beard C; Sorcinelli MD; Ong CC; Korsmeyer SJ. 2003. Development and maintenance of B and T lymphocytes requires antiapoptotic MCL-1. Nature 426(6967):671-6. [PubMed: 14668867] [MGI Ref ID J:86906]
Otero DC; Anzelon AN; Rickert RC. 2003. CD19 function in early and late B cell development: I. Maintenance of follicular and marginal zone B cells requires CD19-dependent survival signals. J Immunol 170(1):73-83. [PubMed: 12496385] [MGI Ref ID J:127029]
Otero DC; Rickert RC. 2003. CD19 function in early and late B cell development. II. CD19 facilitates the pro-B/pre-B transition. J Immunol 171(11):5921-30. [PubMed: 14634103] [MGI Ref ID J:118748]
Pao LI; Lam KP; Henderson JM; Kutok JL; Alimzhanov M; Nitschke L; Thomas ML; Neel BG; Rajewsky K. 2007. B cell-specific deletion of protein-tyrosine phosphatase shp1 promotes B-1a cell development and causes systemic autoimmunity. Immunity 27(1):35-48. [PubMed: 17600736] [MGI Ref ID J:123568]
Pasparakis M; Schmidt-Supprian M; Rajewsky K. 2002. IkappaB kinase signaling is essential for maintenance of mature B cells. J Exp Med 196(6):743-52. [PubMed: 12235208] [MGI Ref ID J:79132]
Polli M; Dakic A; Light A; Wu L; Tarlinton DM; Nutt SL. 2005. The development of functional B lymphocytes in conditional PU.1 knock-out mice. Blood 106(6):2083-90. [PubMed: 15933053] [MGI Ref ID J:118870]
Pongratz G; McAlees JW; Conrad DH; Erbe RS; Haas KM; Sanders VM. 2006. The level of IgE produced by a B cell is regulated by norepinephrine in a p38 MAPK- and CD23-dependent manner. J Immunol 177(5):2926-38. [PubMed: 16920928] [MGI Ref ID J:139551]
Prinz M; Schmidt H; Mildner A; Knobeloch KP; Hanisch UK; Raasch J; Merkler D; Detje C; Gutcher I; Mages J; Lang R; Martin R; Gold R; Becher B; Bruck W; Kalinke U. 2008. Distinct and nonredundant in vivo functions of IFNAR on myeloid cells limit autoimmunity in the central nervous system. Immunity 28(5):675-86. [PubMed: 18424188] [MGI Ref ID J:136301]
Rastelli J; Homig-Holzel C; Seagal J; Muller W; Hermann AC; Rajewsky K; Zimber-Strobl U. 2008. LMP1 signaling can replace CD40 signaling in B cells in vivo and has unique features of inducing class-switch recombination to IgG1. Blood 111(3):1448-55. [PubMed: 18006702] [MGI Ref ID J:130768]
Rickert RC; Rajewsky K; Roes J. 1995. Impairment of T-cell-dependent B-cell responses and B-1 cell development in CD19-deficient mice. Nature 376(6538):352-5. [PubMed: 7543183] [MGI Ref ID J:27463]
Rieken S; Herroeder S; Sassmann A; Wallenwein B; Moers A; Offermanns S; Wettschureck N. 2006. Lysophospholipids control integrin-dependent adhesion in splenic B cells through G(i) and G(12)/G(13) family G-proteins but not through G(q)/G(11). J Biol Chem 281(48):36985-92. [PubMed: 17023430] [MGI Ref ID J:117663]
Roes J; Choi BK; Cazac BB. 2003. Redirection of B cell responsiveness by transforming growth factor beta receptor. Proc Natl Acad Sci U S A 100(12):7241-6. [PubMed: 12773615] [MGI Ref ID J:95504]
Saito T; Chiba S; Ichikawa M; Kunisato A; Asai T; Shimizu K; Yamaguchi T; Yamamoto G; Seo S; Kumano K; Nakagami-Yamaguchi E; Hamada Y; Aizawa S; Hirai H. 2003. Notch2 is preferentially expressed in mature B cells and indispensable for marginal zone B lineage development. Immunity 18(5):675-85. [PubMed: 12753744] [MGI Ref ID J:83476]
Sanjo H; Hikida M; Aiba Y; Mori Y; Hatano N; Ogata M; Kurosaki T. 2007. Extracellular signal-regulated protein kinase 2 is required for efficient generation of B cells bearing antigen-specific immunoglobulin G. Mol Cell Biol 27(4):1236-46. [PubMed: 17145771] [MGI Ref ID J:118279]
Sanyal M; Tung JW; Karsunky H; Zeng H; Selleri L; Weissman IL; Herzenberg LA; Cleary ML. 2007. B-cell development fails in the absence of the Pbx1 proto-oncogene. Blood 109(10):4191-9. [PubMed: 17244677] [MGI Ref ID J:132963]
Sasaki Y; Calado DP; Derudder E; Zhang B; Shimizu Y; Mackay F; Nishikawa S; Rajewsky K; Schmidt-Supprian M. 2008. NIK overexpression amplifies, whereas ablation of its TRAF3-binding domain replaces BAFF:BAFF-R-mediated survival signals in B cells. Proc Natl Acad Sci U S A 105(31):10883-8. [PubMed: 18663224] [MGI Ref ID J:139293]
Sasaki Y; Derudder E; Hobeika E; Pelanda R; Reth M; Rajewsky K; Schmidt-Supprian M. 2006. Canonical NF-kappaB activity, dispensable for B cell development, replaces BAFF-receptor signals and promotes B cell proliferation upon activation. Immunity 24(6):729-39. [PubMed: 16782029] [MGI Ref ID J:113365]
Sato S; Sanjo H; Takeda K; Ninomiya-Tsuji J; Yamamoto M; Kawai T; Matsumoto K; Takeuchi O; Akira S. 2005. Essential function for the kinase TAK1 in innate and adaptive immune responses. Nat Immunol 6(11):1087-95. [PubMed: 16186825] [MGI Ref ID J:112597]
Savitsky D; Calame K. 2006. B-1 B lymphocytes require Blimp-1 for immunoglobulin secretion. J Exp Med 203(10):2305-14. [PubMed: 16954370] [MGI Ref ID J:124626]
Schebesta A; McManus S; Salvagiotto G; Delogu A; Busslinger GA; Busslinger M. 2007. Transcription factor Pax5 activates the chromatin of key genes involved in B cell signaling, adhesion, migration, and immune function. Immunity 27(1):49-63. [PubMed: 17658281] [MGI Ref ID J:123629]
Schmidt-Supprian M; Wunderlich FT; Rajewsky K. 2007. Excision of the Frt-flanked neo ( R ) cassette from the CD19cre knock-in transgene reduces Cre-mediated recombination. Transgenic Res 16(5):657-60. [PubMed: 17541717] [MGI Ref ID J:125035]
Shapiro-Shelef M; Lin KI; McHeyzer-Williams LJ; Liao J; McHeyzer-Williams MG; Calame K. 2003. Blimp-1 is required for the formation of immunoglobulin secreting plasma cells and pre-plasma memory B cells. Immunity 19(4):607-20. [PubMed: 14563324] [MGI Ref ID J:101890]
Shih TA; Roederer M; Nussenzweig MC. 2002. Role of antigen receptor affinity in T cell-independent antibody responses in vivo. Nat Immunol 3(4):399-406. [PubMed: 11896394] [MGI Ref ID J:75653]
Shimoda M; Li T; Pihkala JP; Koni PA. 2006. Role of MHC class II on memory B cells in post-germinal center B cell homeostasis and memory response. J Immunol 176(4):2122-33. [PubMed: 16455968] [MGI Ref ID J:129125]
Stranges PB; Watson J; Cooper CJ; Choisy-Rossi CM; Stonebraker AC; Beighton RA; Hartig H; Sundberg JP; Servick S; Kaufmann G; Fink PJ; Chervonsky AV. 2007. Elimination of antigen-presenting cells and autoreactive T cells by Fas contributes to prevention of autoimmunity. Immunity 26(5):629-41. [PubMed: 17509906] [MGI Ref ID J:123556]
Suzuki A; Kaisho T; Ohishi M; Tsukio-Yamaguchi M; Tsubata T; Koni PA; Sasaki T; Mak TW; Nakano T. 2003. Critical roles of Pten in B cell homeostasis and immunoglobulin class switch recombination. J Exp Med 197(5):657-67. [PubMed: 12615906] [MGI Ref ID J:91501]
Takeuchi O; Fisher J; Suh H; Harada H; Malynn BA; Korsmeyer SJ. 2005. Essential role of BAX,BAK in B cell homeostasis and prevention of autoimmune disease. Proc Natl Acad Sci U S A 102(32):11272-7. [PubMed: 16055554] [MGI Ref ID J:100463]
Tanigaki K; Han H; Yamamoto N; Tashiro K; Ikegawa M; Kuroda K; Suzuki A; Nakano T; Honjo T. 2002. Notch-RBP-J signaling is involved in cell fate determination of marginal zone B cells. Nat Immunol 3(5):443-50. [PubMed: 11967543] [MGI Ref ID J:76240]
Thomas MD; Kremer CS; Ravichandran KS; Rajewsky K; Bender TP. 2005. c-Myb is critical for B cell development and maintenance of follicular B cells. Immunity 23(3):275-86. [PubMed: 16169500] [MGI Ref ID J:113271]
Tokoyoda K; Egawa T; Sugiyama T; Choi BI; Nagasawa T. 2004. Cellular niches controlling B lymphocyte behavior within bone marrow during development. Immunity 20(6):707-18. [PubMed: 15189736] [MGI Ref ID J:109147]
Tumanov A; Kuprash D; Lagarkova M; Grivennikov S; Abe K; Shakhov A; Drutskaya L; Stewart C; Chervonsky A; Nedospasov S. 2002. Distinct role of surface lymphotoxin expressed by B cells in the organization of secondary lymphoid tissues. Immunity 17(3):239-50. [PubMed: 12354378] [MGI Ref ID J:79129]
Tumanov AV; Grivennikov SI; Shakhov AN; Rybtsov SA; Koroleva EP; Takeda J; Nedospasov SA; Kuprash DV. 2003. Dissecting the role of lymphotoxin in lymphoid organs by conditional targeting. Immunol Rev 195:106-16. [PubMed: 12969314] [MGI Ref ID J:85493]
Twohig J; Kulik L; Haluszczak C; Reuter J; Rossbach A; Bull M; Holers VM; Marchbank KJ. 2007. Defective B cell ontogeny and immune response in human complement receptor 2 (CR2, CD21) transgenic mice is partially recovered in the absence of C3. Mol Immunol 44(13):3434-44. [PubMed: 17379312] [MGI Ref ID J:123562]
Veillette A; Zhang S; Shi X; Dong Z; Davidson D; Zhong MC. 2008. SAP expression in T cells, not in B cells, is required for humoral immunity. Proc Natl Acad Sci U S A 105(4):1273-8. [PubMed: 18212118] [MGI Ref ID J:131618]
Walmsley MJ; Ooi SK; Reynolds LF; Smith SH; Ruf S; Mathiot A; Vanes L; Williams DA; Cancro MP; Tybulewicz VL. 2003. Critical roles for Rac1 and Rac2 GTPases in B cell development and signaling. Science 302(5644):459-62. [PubMed: 14564011] [MGI Ref ID J:86765]
Wang Y; Brooks SR; Li X; Anzelon AN; Rickert RC; Carter RH. 2002. The Physiologic Role of CD19 Cytoplasmic Tyrosines. Immunity 17(4):501. [PubMed: 12387743] [MGI Ref ID J:79571]
Weber M; Treanor B; Depoil D; Shinohara H; Harwood NE; Hikida M; Kurosaki T; Batista FD. 2008. Phospholipase C-gamma2 and Vav cooperate within signaling microclusters to propagate B cell spreading in response to membrane-bound antigen. J Exp Med 205(4):853-68. [PubMed: 18362175] [MGI Ref ID J:133999]
Wilker PR; Kohyama M; Sandau MM; Albring JC; Nakagawa O; Schwarz JJ; Murphy KM. 2008. Transcription factor Mef2c is required for B cell proliferation and survival after antigen receptor stimulation. Nat Immunol 9(6):603-12. [PubMed: 18438409] [MGI Ref ID J:136292]
Winslow MM; Gallo EM; Neilson JR; Crabtree GR. 2006. The calcineurin phosphatase complex modulates immunogenic B cell responses. Immunity 24(2):141-52. [PubMed: 16473827] [MGI Ref ID J:113318]
Xie P; Stunz LL; Larison KD; Yang B; Bishop GA. 2007. Tumor necrosis factor receptor-associated factor 3 is a critical regulator of B cell homeostasis in secondary lymphoid organs. Immunity 27(2):253-67. [PubMed: 17723217] [MGI Ref ID J:124329]
Xu W; Fukuyama T; Ney PA; Wang D; Rehg J; Boyd K; van Deursen JM; Brindle PK. 2006. Global transcriptional coactivators CREB-binding protein and p300 are highly essential collectively but not individually in peripheral B cells. Blood 107(11):4407-16. [PubMed: 16424387] [MGI Ref ID J:123532]
Xu Y; Beavitt SJ; Harder KW; Hibbs ML; Tarlinton DM. 2002. The activation and subsequent regulatory roles of Lyn and CD19 after B cell receptor ligation are independent. J Immunol 169(12):6910-8. [PubMed: 12471124] [MGI Ref ID J:118420]
Yabe D; Fukuda H; Aoki M; Yamada S; Takebayashi S; Shinkura R; Yamamoto N; Honjo T. 2007. Generation of a conditional knockout allele for mammalian Spen protein Mint/SHARP. Genesis 45(5):300-6. [PubMed: 17457934] [MGI Ref ID J:121523]
Yagi T; Giallourakis C; Mohanty S; Scheidig C; Shen J; Zheng H; Xavier RJ; Shaw AC. 2008. Defective signal transduction in B lymphocytes lacking presenilin proteins. Proc Natl Acad Sci U S A 105(3):979-84. [PubMed: 18195359] [MGI Ref ID J:131284]
Yamamoto M; Okamoto T; Takeda K; Sato S; Sanjo H; Uematsu S; Saitoh T; Yamamoto N; Sakurai H; Ishii KJ; Yamaoka S; Kawai T; Matsuura Y; Takeuchi O; Akira S. 2006. Key function for the Ubc13 E2 ubiquitin-conjugating enzyme in immune receptor signaling. Nat Immunol 7(9):962-70. [PubMed: 16862162] [MGI Ref ID J:112287]
Yu Q; Quinn WJ rd; Salay T; Crowley JE; Cancro MP; Sen JM. 2008. Role of beta-catenin in B cell development and function. J Immunol 181(6):3777-83. [PubMed: 18768830] [MGI Ref ID J:139115]
Yusuf I; Kharas MG; Chen J; Peralta RQ; Maruniak A; Sareen P; Yang VW; Kaestner KH; Fruman DA. 2008. KLF4 is a FOXO target gene that suppresses B cell proliferation. Int Immunol 20(5):671-81. [PubMed: 18375530] [MGI Ref ID J:134502]
de Alboran IM; Baena E; Martinez-A C. 2004. c-Myc-deficient B lymphocytes are resistant to spontaneous and induced cell death. Cell Death Differ 11(1):61-8. [PubMed: 12970677] [MGI Ref ID J:115648]
de Alboran IM; O'Hagan RC; Gartner F; Malynn B; Davidson L; Rickert R; Rajewsky K; DePinho RA; Alt FW. 2001. Analysis of C-MYC function in normal cells via conditional gene-targeted mutation. Immunity 14(1):45-55. [PubMed: 11163229] [MGI Ref ID J:67222]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice may be bred as heterozygotes or homozygotes. The donating investigator reports that homozygotes older than 3 months of age do not breed well. Homozygous breeding pairs maintained at The Jackson Laboratory have shown no such problems. Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $155.70 Female or Male Homozygous for Cd19tm1(cre)Cgn *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $311.40 Homozygous for Cd19tm1(cre)Cgn x Homozygous for Cd19tm1(cre)Cgn
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
|
Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $202.50 Female or Male Homozygous for Cd19tm1(cre)Cgn *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $404.90 Homozygous for Cd19tm1(cre)Cgn x Homozygous for Cd19tm1(cre)Cgn
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
|
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
- Use of MICE by companies or for-profit entities requires a license.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form