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Strain Common Names	DH;
Genes & Alleles	Del(14)3Mom;

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Product Information

Strain Details

Type Chromosome Aberration Additional information on Mice with Chromosomal Aberrations. Type Deletion Additional information on Mice with Chromosomal Aberrations. Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Targeted Mutation Mating System Homozygote x Homozygote         (Female x Male) Species laboratory mouse Donating Investigator Peter Mombaerts,   Max Planck Institute of Biophysics Generation ?+F3 (25-APR-08)

Strain Description
The H element was identified by the high homoology of this non-coding DNA between mouse and human. In hemizygotes, the Del(14)3Mom deletion of the H element abolishes expression of Olfr1507 (MOR28) and Olfr1508 (MOR10) genes in cis from the 129P2/OlaHsd chromosome, while expression of Olfr1507 and Olfr1508 genes from the wildtype C57BL/6J chromosome persists. In homozygotes, Olfr1507, Olfr1508, and Olfr1509 (MOR83) genes are not expressed, and there is a graded, distance-related decrease in the numbers of cells expressing more distal olfactory receptor genes, Olfr1510 (MOR29A), Olfr1511 (MOR29B), Olfr1512 (MOR30A), and Olfr1513 (MOR30B). The expression of other olfactory receptor genes on Chromosome 14 such as Olfr1514 (MOR205-1), Olfr749 ((S1), and Olfr221 (MOR205-1) is not affected in an obvious way by the Del(14)3Mom deletion. Expression of olfactory receptor genes located on other chromosomes, including Olfr6 (M50), Olfr17 (P2), Olfr16 (MOR23), Olfr151 (M71), and Olfr15 (MOR256-17), is not impacted. Thus, deletion of the H element has a local and cis effect on the expression of olfactory receptor genes.

Strain Development
A 2,119 nucleotide ScaI-flanked sequence of the H element was replaced with the ACNF neomycin resistance cassette via homologous recombination in 129P2/OlaHsd derived E14 ES cells and this neomycin resistance cassette self-excised in the male germline. Male chimeras were bred to C57BL/6J females. This line has been maintained on this mixed B6;129P2 background and was intercrossed to homozygosity. In 2007 sperm from homozygous males was cryopreserved.

The plasmid that was used to generate this mutation is banked and described at Addgene.

Mammalian Phenotype Terms assigned by genotype Del(14)3Mom/+         (C57BL/6J x 129P2/OlaHsd)F1 taste/olfaction phenotype abnormal olfactory receptor morphology (MGI Ref ID J:122925) loss or diminished expression of a subset of olfactory receptors that are on the same chromosome as the deletion, with less impact on olfactory receptor genes with increased chromosomal distance from the deletion and no impact on expression of olfactory receptors expressed in trans nervous system phenotype abnormal olfactory receptor morphology (MGI Ref ID J:122925) loss or diminished expression of a subset of olfactory receptors that are on the same chromosome as the deletion, with less impact on olfactory receptor genes with increased chromosomal distance from the deletion and no impact on expression of olfactory receptors expressed in trans Del(14)3Mom/Del(14)3Mom         involves: 129P2/OlaHsd * C57BL/6J taste/olfaction phenotype *normal* taste/olfaction phenotype (MGI Ref ID J:122925) Despite the absence of the H element, homozygotes express most olfactory receptor genes and olfactory receptor-specific coalescence of axons into glomeruli is normal abnormal olfactory receptor morphology (MGI Ref ID J:122925) abolished expression of Olfr1507, Olfr1508, and Olfr1509, and a tapered reduction in expression of some of the more distal Chromosome 14 olfactory receptors nervous system phenotype abnormal olfactory receptor morphology (MGI Ref ID J:122925) abolished expression of Olfr1507, Olfr1508, and Olfr1509, and a tapered reduction in expression of some of the more distal Chromosome 14 olfactory receptors

Gene & Allele Details

Allele Symbol		Del(14)3Mom
Allele Name		deletion, Chr 14, Peter Mombaerts 3
Common Name(s)		DH; Del3Mom; deltaH;
Mutation Made By		Peter Mombaerts,   Max Planck Institute of Biophysics
Strain of Origin		129P2/OlaHsd
ES Cell Line Name		E14
ES Cell Line Strain		129P2/OlaHsd
Molecular Note		The H element was deleted by replacing the 2,119 nucleotide ScaI flanked segment with the ACNF neomycin resistance cassette via homologous recombination and the neomycin resistance cassette was self-excised during germline transmission. [MGI Ref ID J:122925]

Control Information

	Control
	None Available
Considerations for Choosing Controls

Genotyping Protocols

Del(14)3Mom

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, homozygous mice may be bred.
Diet Information	LabDiet® 5K52/5K67

Animal Health Reports

Room Number           AX11

References

Selected Reference(s)

Fuss SH; Omura M; Mombaerts P. 2007. Local and cis effects of the H element on expression of odorant receptor genes in mouse Cell 130(2):373-384. [PubMed: 17662950]  [MGI Ref ID J:122925]

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Price and Supply Information

Strain Name:	B6;129P2-Del(14)3Mom/MomJ
Stock Number:	006790

Price Details

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Supply Details

Standard Supply	Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes	Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource.
Licensing	See General Terms and Conditions below for Licensing and Use Restrictions
Control Information	View Control Information in Strain Details.

General Terms and Conditions

View JAX^® Mice & Services Conditions of Use.

Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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