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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Chromosome Aberration; Deletion; Additional information on Mice with Chromosomal Aberrations. Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation ?+F3pN1
Generation DefinitionsDonating Investigator Peter Mombaerts, Max Planck Institute of Biophysics Description
The H element was identified by the high homoology of this non-coding DNA between mouse and human. In hemizygotes, the Del(14)3Mom deletion of the H element abolishes expression of Olfr1507 (MOR28) and Olfr1508 (MOR10) genes in cis from the 129P2/OlaHsd chromosome, while expression of Olfr1507 and Olfr1508 genes from the wild-type C57BL/6J chromosome persists. In homozygotes, Olfr1507, Olfr1508, and Olfr1509 (MOR83) genes are not expressed, and there is a graded, distance-related decrease in the numbers of cells expressing more distal olfactory receptor genes, Olfr1510 (MOR29A), Olfr1511 (MOR29B), Olfr1512 (MOR30A), and Olfr1513 (MOR30B). The expression of other olfactory receptor genes on Chromosome 14 such as Olfr1514 (MOR205-1), Olfr749 ((S1), and Olfr221 (MOR205-1) is not affected in an obvious way by the Del(14)3Mom deletion. Expression of olfactory receptor genes located on other chromosomes, including Olfr6 (M50), Olfr17 (P2), Olfr16 (MOR23), Olfr151 (M71), and Olfr15 (MOR256-17), is not impacted. Thus, deletion of the H element has a local and cis effect on the expression of olfactory receptor genes.Development
A 2,119 nucleotide ScaI-flanked sequence of the H element was replaced with the ACNF neomycin resistance cassette via homologous recombination in 129P2/OlaHsd derived E14 ES cells and this neomycin resistance cassette self-excised in the male germline. Male chimeras were bred to C57BL/6J females. This line has been maintained on this mixed B6;129P2 background and was intercrossed to homozygosity. In 2007 sperm from homozygous males was cryopreserved.The plasmid that was used to generate this mutation is banked and described at Addgene.
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
Phenotype
Phenotype Information
View Mammalian Phenotype Terms
Mammalian Phenotype Terms provided by MGI
assigned by genotype
Del(14)3Mom/+
(C57BL/6J x 129P2/OlaHsd)F1
- taste/olfaction phenotype
- abnormal olfactory receptor morphology
- loss or diminished expression of a subset of olfactory receptors that are on the same chromosome as the deletion, with less impact on olfactory receptor genes with increased chromosomal distance from the deletion and no impact on expression of olfactory receptors expressed in trans (MGI Ref ID J:122925)
- nervous system phenotype
- abnormal olfactory receptor morphology
- loss or diminished expression of a subset of olfactory receptors that are on the same chromosome as the deletion, with less impact on olfactory receptor genes with increased chromosomal distance from the deletion and no impact on expression of olfactory receptors expressed in trans (MGI Ref ID J:122925)
Del(14)3Mom/Del(14)3Mom
involves: 129P2/OlaHsd * C57BL/6J
- taste/olfaction phenotype
- *normal* taste/olfaction phenotype
- Despite the absence of the H element, homozygotes express most olfactory receptor genes and olfactory receptor-specific coalescence of axons into glomeruli is normal (MGI Ref ID J:122925)
- abnormal olfactory receptor morphology
- abolished expression of Olfr1507, Olfr1508, and Olfr1509, and a tapered reduction in expression of some of the more distal Chromosome 14 olfactory receptors (MGI Ref ID J:122925)
- nervous system phenotype
- abnormal olfactory receptor morphology
- abolished expression of Olfr1507, Olfr1508, and Olfr1509, and a tapered reduction in expression of some of the more distal Chromosome 14 olfactory receptors (MGI Ref ID J:122925)
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Del(14)3Mom | ||
|---|---|---|---|
| Allele Name | deletion, Chr 14, Peter Mombaerts 3 | ||
| Allele Type | Targeted (knock-out) | ||
| Common Name(s) | DH; Del3Mom; deltaH; | ||
| Mutation Made By | Peter Mombaerts, Max Planck Institute of Biophysics | ||
| Strain of Origin | 129P2/OlaHsd | ||
| ES Cell Line Name | E14 | ||
| ES Cell Line Strain | 129P2/OlaHsd | ||
| Gene Symbol and Name | Del(14)3Mom, deletion, Chr 14, Peter Mombaerts 3 | ||
| Chromosome | 14 | ||
| Associated Marker Note | Affected-Count: 5Af1-Gene: MGI:3031341 Af2-Gene: MGI:3031342 Af3-Gene: MGI:3031343Af4-Gene: MGI:3030385 Af5-Gene: MGI:3030403 | ||
| Molecular Note | The H element was deleted by replacing the 2,119 nucleotide ScaI flanked segment with the ACNF neomycin resistance cassette via homologous recombination and the neomycin resistance cassette was self-excised during germline transmission. [MGI Ref ID J:122925] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Del(14)3Mom, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Fuss SH; Omura M; Mombaerts P. 2007. Local and cis effects of the H element on expression of odorant receptor genes in mouse Cell 130(2):373-384. [PubMed: 17662950] [MGI Ref ID J:122925]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, RG10/RG30.Colony Maintenance
Breeding & Husbandry When maintaining a live colony, homozygous mice may be bred.
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
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