Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N13pN1 Generation Definitions   Donating Investigator Eva Eicher,   The Jackson Laboratory

Development
This mutation was generated in R1 ES cells, which were derived from (129X1/SvJ x 129S1/Sv)F1-Kitl⁺, and the resulting mice were bred with C57BL/6. In 2003 mice were imported into the laboratory of Dr. Eva Eicher at The Jackson Laboratory from Dr. Heiner Wesphal at NCI, and were bred to C57BL/6J for rederivation then backcrossed on to DBA/2J. In 2007 sperm were cryopreserved from heterozygous males at generation N13.

Related Strains

Strains carrying   Lhx9^tm1Lmgd allele

006812

B6Ei.129(Cg)-Lhx9tm1Lmgd/EiJ

View Strains carrying   Lhx9^tm1Lmgd     (1 strain)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Lhx9^tm1Lmgd/Lhx9^tm1Lmgd

        involves: 129S1/Sv * 129X1/SvJ * C57BL/6

• endocrine/exocrine gland phenotype
• agonadal
  • all mice lacked gonads; at E13.5, no gonads are apparent   (MGI Ref ID J:60621)
• • absent ovary   (MGI Ref ID J:60621)
  • absent testes   (MGI Ref ID J:60621)
• homeostasis/metabolism phenotype
• decreased circulating estradiol level
  • undetectable levels of estradiol   (MGI Ref ID J:60621)
• decreased circulating testosterone level
  • undetectable levels of testosterone   (MGI Ref ID J:60621)
• increased circulating follicle stimulating hormone level
  • elevated levels of FSH   (MGI Ref ID J:60621)
• reproductive system phenotype
• abnormal female reproductive system morphology
  • atrophic   (MGI Ref ID J:60621)
• • uterus atrophy   (MGI Ref ID J:60621)
  • vagina atrophy   (MGI Ref ID J:60621)
• abnormal male reproductive system morphology
  • no male accessory sex organs found in genetically identified males   (MGI Ref ID J:60621)
• abnormal primary sex determination
  • all mice phenotypically female, although half were genetically male   (MGI Ref ID J:60621)
• • agonadal
    • all mice lacked gonads; at E13.5, no gonads are apparent   (MGI Ref ID J:60621)
  • • absent ovary   (MGI Ref ID J:60621)
    • absent testes   (MGI Ref ID J:60621)
• abnormal primordial germ cell proliferation
  • primordial germ cells migrate to the gonadal region at E12.0, but the proliferation of gonadal cells at E11.5 was not apparent   (MGI Ref ID J:60621)
• absent estrous cycle
  • females did not undergo an oestrus cycle   (MGI Ref ID J:60621)
• infertility
  • mice were infertile   (MGI Ref ID J:60621)
• • female infertility   (MGI Ref ID J:60621)
  • male infertility   (MGI Ref ID J:60621)
• secondary sex reversal
  • all mice phenotypically female, although half were genetically male   (MGI Ref ID J:60621)
  • no male accessory sex organs found in genetically identified males   (MGI Ref ID J:60621)
• cellular phenotype
• abnormal primordial germ cell proliferation
  • primordial germ cells migrate to the gonadal region at E12.0, but the proliferation of gonadal cells at E11.5 was not apparent   (MGI Ref ID J:60621)

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Lhx9tm1Lmgd
Allele Name		targeted mutation 1, Laboratory of Mammalian Genes and Development, Heiner Westphal
Allele Type		Targeted (knock-out)
Common Name(s)		Lhx9-;
Mutation Made By		Linda Washburn,   The Jackson Laboratory
Strain of Origin		(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
ES Cell Line Name		R1
ES Cell Line Strain		(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Gene Symbol and Name		Lhx9, LIM homeobox protein 9
Chromosome		1
Gene Common Name(s)		3110009O07Rik; BB104635; LH2B; Lhx9 alpha; RIKEN cDNA 3110009O07 gene; expressed sequence BB104635;
Molecular Note		A neomycin resistance cassette replaced exons 2 and 3 of the gene, which encode the first and second LIM domains of the protein. [MGI Ref ID J:60621]

Genotyping

Genotyping Information

Genotyping Protocols

Lhx9^tm1Lmgd, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Birk OS; Casiano DE; Wassif CA; Cogliati T; Zhao L; Zhao Y; Grinberg A; Huang S; Kreidberg JA; Parker KL; Porter FD; Westphal H. 2000. The LIM homeobox gene Lhx9 is essential for mouse gonad formation. Nature 403(6772):909-13. [PubMed: 10706291]  [MGI Ref ID J:60621]

Additional References

Lhx9^tm1Lmgd related

Brennan J; Capel B. 2004. One tissue, two fates: molecular genetic events that underlie testis versus ovary development. Nat Rev Genet 5(7):509-21. [PubMed: 15211353]  [MGI Ref ID J:90770]

Tzchori I; Day TF; Carolan PJ; Zhao Y; Wassif CA; Li L; Lewandoski M; Gorivodsky M; Love PE; Porter FD; Westphal H; Yang Y. 2009. LIM homeobox transcription factors integrate signaling events that control three-dimensional limb patterning and growth. Development 136(8):1375-85. [PubMed: 19304889]  [MGI Ref ID J:147278]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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