Strain Name:

D2.129(Cg)-Lhx9tm1Lmgd/EiJ

Stock Number:

006811

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Availability:

Cryopreserved - Ready for recovery

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
GenerationN13pN1
Generation Definitions
 
Donating Investigator Eva Eicher,   The Jackson Laboratory

Development
This mutation was generated in R1 ES cells, which were derived from (129X1/SvJ x 129S1/Sv)F1-Kitl+, and the resulting mice were bred with C57BL/6. In 2003 mice were imported into the laboratory of Dr. Eva Eicher at The Jackson Laboratory from Dr. Heiner Wesphal at NCI, and were bred to C57BL/6J for rederivation then backcrossed on to DBA/2J. In 2007 sperm were cryopreserved from heterozygous males at generation N13.

Related Strains

Strains carrying   Lhx9tm1Lmgd allele
006812   B6Ei.129(Cg)-Lhx9tm1Lmgd/EiJ
View Strains carrying   Lhx9tm1Lmgd     (1 strain)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Lhx9tm1Lmgd/Lhx9tm1Lmgd

        involves: 129S1/Sv * 129X1/SvJ * C57BL/6
  • endocrine/exocrine gland phenotype
  • agonadal
    • all mice lacked gonads; at E13.5, no gonads are apparent   (MGI Ref ID J:60621)
    • absent ovary   (MGI Ref ID J:60621)
    • absent testes   (MGI Ref ID J:60621)
  • homeostasis/metabolism phenotype
  • decreased circulating estradiol level
    • undetectable levels of estradiol   (MGI Ref ID J:60621)
  • decreased circulating testosterone level
    • undetectable levels of testosterone   (MGI Ref ID J:60621)
  • increased circulating follicle stimulating hormone level
    • elevated levels of FSH   (MGI Ref ID J:60621)
  • reproductive system phenotype
  • abnormal female reproductive system morphology
    • uterus atrophy   (MGI Ref ID J:60621)
    • vagina atrophy   (MGI Ref ID J:60621)
  • abnormal male reproductive system morphology
    • no male accessory sex organs found in genetically identified males   (MGI Ref ID J:60621)
  • abnormal primary sex determination
    • all mice phenotypically female, although half were genetically male   (MGI Ref ID J:60621)
    • agonadal
      • all mice lacked gonads; at E13.5, no gonads are apparent   (MGI Ref ID J:60621)
      • absent ovary   (MGI Ref ID J:60621)
      • absent testes   (MGI Ref ID J:60621)
  • abnormal primordial germ cell proliferation
    • primordial germ cells migrate to the gonadal region at E12.0, but the proliferation of gonadal cells at E11.5 was not apparent   (MGI Ref ID J:60621)
  • absent estrous cycle
    • females did not undergo an oestrus cycle   (MGI Ref ID J:60621)
  • infertility
    • mice were infertile   (MGI Ref ID J:60621)
    • female infertility   (MGI Ref ID J:60621)
    • male infertility   (MGI Ref ID J:60621)
  • secondary sex reversal
    • all mice phenotypically female, although half were genetically male   (MGI Ref ID J:60621)
    • no male accessory sex organs found in genetically identified males   (MGI Ref ID J:60621)
  • cellular phenotype
  • abnormal primordial germ cell proliferation
    • primordial germ cells migrate to the gonadal region at E12.0, but the proliferation of gonadal cells at E11.5 was not apparent   (MGI Ref ID J:60621)

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Lhx9tm1Lmgd
Allele Name targeted mutation 1, Laboratory of Mammalian Genes and Development, Heiner Westphal
Allele Type Targeted (Null/Knockout)
Common Name(s) Lhx9-;
Mutation Made By Linda Washburn,   The Jackson Laboratory
Strain of Origin(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
ES Cell Line NameR1
ES Cell Line Strain(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Gene Symbol and Name Lhx9, LIM homeobox protein 9
Chromosome 1
Gene Common Name(s) 3110009O07Rik; BB104635; LH2B; Lhx9 alpha; RIKEN cDNA 3110009O07 gene; expressed sequence BB104635;
Molecular Note A neomycin resistance cassette replaced exons 2 and 3 of the gene, which encode the first and second LIM domains of the protein. [MGI Ref ID J:60621]

Genotyping

Genotyping Information

Genotyping Protocols

Lhx9tm1Lmgd, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Birk OS; Casiano DE; Wassif CA; Cogliati T; Zhao L; Zhao Y; Grinberg A; Huang S; Kreidberg JA; Parker KL; Porter FD; Westphal H. 2000. The LIM homeobox gene Lhx9 is essential for mouse gonad formation. Nature 403(6772):909-13. [PubMed: 10706291]  [MGI Ref ID J:60621]

Additional References

Lhx9tm1Lmgd related

Brennan J; Capel B. 2004. One tissue, two fates: molecular genetic events that underlie testis versus ovary development. Nat Rev Genet 5(7):509-21. [PubMed: 15211353]  [MGI Ref ID J:90770]

Tzchori I; Day TF; Carolan PJ; Zhao Y; Wassif CA; Li L; Lewandoski M; Gorivodsky M; Love PE; Porter FD; Westphal H; Yang Y. 2009. LIM homeobox transcription factors integrate signaling events that control three-dimensional limb patterning and growth. Development 136(8):1375-85. [PubMed: 19304889]  [MGI Ref ID J:147278]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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