Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse   Donating Investigator David Clapham,   HHMI/Children's Hospital

Description
Mice homozygous for this targeted mutation are viable and do not display any gross physical or behavioral abnormalities. Male homozygotes are infertile due to a defect in sperm motility. Their sperm fails to develop the hyperactive motility pattern required for fertilization. No morphological differences between wildtype and mutant spermatozoa have been observed. No protein product from the targeted gene is detected in testis tissue. Female homozygotes reproduce normally. This strain may be useful in studies of male infertility.

Development
A targeting vector containing a neomycin resistance gene was used to replace exons 4-7. The construct was electroporated into 129S4/SvJae-derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Chimeric animals were crossed with 129S1/Sv. The strain was backcrossed four times to 129S1/Sv by the donating laboratory.

Control Information

	Control
	002448 129S1/SvImJ	(approximate)
Considerations for Choosing Controls

Additional Web Information

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Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

Catsper3^tm1Clph/Catsper3^tm1Clph

        involves: 129S4/SvJae

• reproductive system phenotype
• abnormal sperm physiology
  • calcium-selective pH sensitive sperm current (I_CatSper) is not detectable   (MGI Ref ID J:116406)
• • abnormal sperm motility
    • percent motility of mutant sperm decreases more rapidly than wild-type   (MGI Ref ID J:116406)
  • • asthenozoospermia
      • after 90 minutes in viscous conditions, sperm lose their motility compared to wild-type which move more vigorously than initially   (MGI Ref ID J:116406)
  • impaired sperm capacitation
    • many capacitated sperm cells that can not hyperactivate motility exhibit decreased bending in midpiece of proximal flagellum   (MGI Ref ID J:116406)
• male infertility
  • males mated with wild-type females sire no litters   (MGI Ref ID J:116406)
• teratozoospermia
  • maximal bending angle of principal bend in midpiece of flagellum is 47% compared to 92% in wild-type   (MGI Ref ID J:116406)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Reproductive Biology Research
Fertility Defects
      males only

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Catsper3tm1Clph
Allele Name		targeted mutation 1, David E Clapham
Allele Type		Targeted (knock-out)
Mutation Made By		David Clapham,   HHMI/Children's Hospital
Strain of Origin		129S4/SvJae
ES Cell Line Name		J1
ES Cell Line Strain		129S4/SvJae
Gene Symbol and Name		Catsper3, cation channel, sperm associated 3
Chromosome		13
Gene Common Name(s)		4921522D01Rik; CACRC; RIKEN cDNA 4921522D01 gene;
Molecular Note		Exons 4-7, including sequences encoding the putative pore region and part of the C-terminus of the protein, have been replaced by a neomycin resistance cassette. [MGI Ref ID J:116406]

Genotyping

Genotyping Information

Genotyping Protocols

Catsper3^tm1Clph, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Qi H; Moran MM; Navarro B; Chong JA; Krapivinsky G; Krapivinsky L; Kirichok Y; Ramsey IS; Quill TA; Clapman DE. 2007. All four CatSper ion channel proteins are required for male fertility and sperm cell hyperactivated motility Proc Natl Acad Sci U S A :. [PubMed: 17227845]  [MGI Ref ID J:116406]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

Control Information

	Control
	002448 129S1/SvImJ	(approximate)
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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