Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Homozygote         (Female x Male)   03-JAN-08 Species laboratory mouse Generation ?N10N1F9 (06-JAN-09) Generation Definitions   Donating Investigator Craig Gerard,   Childrens' Hospital Boston, Harvard MS

Description
Mice homozygous for the targeted mutation are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities when maintained under barrier conditions. No mRNA is detected by Northern blot analysis of bone marrow cells isolated from homozygotes, and neutrophils fail to respond to recombinant C5a in vitro. The immune response of homozygous mice following inoculation or challenge has been characterized on a C57BL/6 background in a number of studies. Following intratracheal inoculation, mutant mice have impaired bacterial clearance in the lungs, associated with extensive secondary infection, despite increased neutrophil accumulation. Conversely, homozygous mutants are protected against immune-complex-associated injury in the lung, peritoneum, skin, and kidney but not against experimental autoimmune encephalomyelitis. Induced acute pancreatitis and pancreatitis-associated lung injury is more severe in homozygotes compared to wildtype. On a BALB/c background, homozygous mutant mice are more resistant to dermal infection, and lymph node cells have greatly increased antigen recall, as measured by interferon gamma secretion, compared to wild type. Homozygotes fail to develop airway hyper-reactivity in hapten asthma models (background not-identified). This mutant can be used to study many lung-associated diseases, immune complex associated injury, and complement-mediated innate immunity.

Development
A targeting vector containing a mouse phosphoglycerate kinase promoter-driven neomycin resistance gene was used to replace the entire coding region of the endogenous gene. The construct was electroporated into the 129S4/SvJae-derived J1 embryonic stem (ES) cells, and correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric males were crossed to C57BL/6 females. Heterozygotes were mated to C57BL/6 mice for 2 generations and then backcrossed 10 generations to BALB/c before being made homozygous. Upon arrival at The Jackson Laboratory, homozygous mice were bred at least one generation to BALB/cJ (Stock No. 000651).

Control Information

	Control
	000651 BALB/cJ
Considerations for Choosing Controls

Related Strains

Strains carrying   C5ar1^tm1Cge allele

005676

STOCK C5ar1tm1Cge/J

View Strains carrying   C5ar1^tm1Cge     (1 strain)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

C5ar1^tm1Cge/C5ar1^tm1Cge

        involves: 129S4/SvJae

• mortality/aging
• increased sensitivity to induced morbidity/mortality
  • mutants challenged with Psuedomonas aeruginosa succumb in the second day after challenge while no wild-type mice die   (MGI Ref ID J:69177)
• immune system phenotype
• abnormal humoral immune response
  • after injection of chicken egg albumin in the tail and administration of antibodies to egg albumin intratracheally, the null mice exhibit almost complete protection from inflammatory response upon examination of lung tissue   (MGI Ref ID J:35529)
• impaired neutrophil recruitment
  • when peritoneal inflammation is induced with glycogen in mutants, a decreased number of neutrophils is elicited   (MGI Ref ID J:69177)
• increased susceptibility to bacterial infection
  • mutants are unable to clear intrapulmonary-instilled Pseudomonas aeruginosa, despite an increase in neutrophil influx, and succumb to pneumonia   (MGI Ref ID J:69177)
  • however, mutants are able to clear the bacteria when challenged intraperitoneally   (MGI Ref ID J:69177)
  • mutants challenged with a sublethal dose of Pseudomonas become superinfected with secondary bacterial strains   (MGI Ref ID J:69177)

C5ar1^tm1Cge/C5ar1^tm1Cge

        involves: 129S4/SvJae * C57BL/6

• skeleton phenotype
• decreased osteoclast cell number
  • bone marrow cells incubated with 1,25(OH)2 vitamin D3 produce fewer osteoclasts than do cells from controls   (MGI Ref ID J:166640)
• immune system phenotype
• decreased circulating interleukin-6 level
  • Il-6 production after vitamin D3 stimulation is significantly reduced   (MGI Ref ID J:166640)
• decreased osteoclast cell number
  • bone marrow cells incubated with 1,25(OH)2 vitamin D3 produce fewer osteoclasts than do cells from controls   (MGI Ref ID J:166640)
• hematopoietic system phenotype
• decreased osteoclast cell number
  • bone marrow cells incubated with 1,25(OH)2 vitamin D3 produce fewer osteoclasts than do cells from controls   (MGI Ref ID J:166640)
• homeostasis/metabolism phenotype
• decreased circulating interleukin-6 level
  • Il-6 production after vitamin D3 stimulation is significantly reduced   (MGI Ref ID J:166640)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Immunology, Inflammation and Autoimmunity Research
Autoimmunity
      specific complement deficiency

Research Tools
Immunology and Inflammation Research
      genes regulating susceptibility to infectious disease and endotoxin
      specific complement deficiency

C5ar1^tm1Cge related

Immunology, Inflammation and Autoimmunity Research
Autoimmunity
      specific complement deficiency

Research Tools
Immunology and Inflammation Research
      genes regulating susceptibility to infectious disease and endotoxin
      specific complement deficiency

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		C5ar1tm1Cge
Allele Name		targeted mutation 1, Craig Gerard
Allele Type		Targeted (knock-out)
Common Name(s)		C5aR-; C5aRKO;
Mutation Made By		Craig Gerard,   Childrens' Hospital Boston, Harvard MS
Strain of Origin		129S4/SvJae
ES Cell Line Name		J1
ES Cell Line Strain		129S4/SvJae
Gene Symbol and Name		C5ar1, complement component 5a receptor 1
Chromosome		7
Gene Common Name(s)		C5A; C5AR; C5R1; C5r1; CD88; D7Msu1; DNA segment, Chr 7, Michigan State University 1; complement component 5, receptor 1;
Molecular Note		A PGK-neomycin resistance cassette replaced the entire coding region of the gene. Northern analysis of bone marrow cells did not detect expression in homozygous mutant mice. [MGI Ref ID J:69177]

Genotyping

Genotyping Information

Genotyping Protocols

C5ar1^tm1Cge, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Hopken UE; Lu B; Gerard NP; Gerard C. 1996. The C5a chemoattractant receptor mediates mucosal defence to infection. Nature 383(6595):86-9. [PubMed: 8779720]  [MGI Ref ID J:69177]

Additional References

C5ar1^tm1Cge related

Abe T; Hosur KB; Hajishengallis E; Reis ES; Ricklin D; Lambris JD; Hajishengallis G. 2012. Local complement-targeted intervention in periodontitis: proof-of-concept using a C5a receptor (CD88) antagonist. J Immunol 189(11):5442-8. [PubMed: 23089394]  [MGI Ref ID J:190681]

Abonia JP; Friend DS; Austen WG Jr; Moore FD Jr; Carroll MC; Chan R; Afnan J; Humbles A; Gerard C; Knight P; Kanaoka Y; Yasuda S; Morokawa N; Austen KF; Stevens RL; Gurish MF. 2005. Mast cell protease 5 mediates ischemia-reperfusion injury of mouse skeletal muscle. J Immunol 174(11):7285-91. [PubMed: 15905575]  [MGI Ref ID J:98963]

Bao L; Wang Y; Haas M; Quigg RJ. 2011. Distinct roles for C3a and C5a in complement-induced tubulointerstitial injury. Kidney Int 80(5):524-34. [PubMed: 21677637]  [MGI Ref ID J:194719]

Bhatia M; Saluja AK; Singh VP; Frossard JL; Lee HS; Bhagat L; Gerard C; Steer ML. 2001. Complement factor C5a exerts an anti-inflammatory effect in acute pancreatitis and associated lung injury. Am J Physiol Gastrointest Liver Physiol 280(5):G974-8. [PubMed: 11292607]  [MGI Ref ID J:69304]

Binstadt BA; Patel PR; Alencar H; Nigrovic PA; Lee DM; Mahmood U; Weissleder R; Mathis D; Benoist C. 2006. Particularities of the vasculature can promote the organ specificity of autoimmune attack. Nat Immunol 7(3):284-92. [PubMed: 16444258]  [MGI Ref ID J:112604]

Bode J; Dutow P; Sommer K; Janik K; Glage S; Tummler B; Munder A; Laudeley R; Sachse KW; Klos A. 2012. A new role of the complement system: C3 provides protection in a mouse model of lung infection with intracellular Chlamydia psittaci. PLoS One 7(11):e50327. [PubMed: 23189195]  [MGI Ref ID J:194784]

Bozic CR; Lu B; Hopken UE; Gerard C; Gerard NP. 1996. Neurogenic amplification of immune complex inflammation. Science 273(5282):1722-5. [PubMed: 8781237]  [MGI Ref ID J:35529]

Clynes R; Maizes JS; Guinamard R; Ono M; Takai T; Ravetch JV. 1999. Modulation of immune complex-induced inflammation in vivo by the coordinate expression of activation and inhibitory Fc receptors. J Exp Med 189(1):179-85. [PubMed: 9874574]  [MGI Ref ID J:112378]

Dunkelberger J; Zhou L; Miwa T; Song WC. 2012. C5aR expression in a novel GFP reporter gene knockin mouse: implications for the mechanism of action of C5aR signaling in T cell immunity. J Immunol 188(8):4032-42. [PubMed: 22430734]  [MGI Ref ID J:184056]

Fan G; Jiang YP; Lu Z; Martin DW; Kelly DJ; Zuckerman JM; Ballou LM; Cohen IS; Lin RZ. 2005. A transgenic mouse model of heart failure using inducible Galpha q. J Biol Chem 280(48):40337-46. [PubMed: 16210321]  [MGI Ref ID J:104100]

Fang C; Miwa T; Shen H; Song WC. 2007. Complement-dependent enhancement of CD8+ T cell immunity to lymphocytic choriomeningitis virus infection in decay-accelerating factor-deficient mice. J Immunol 179(5):3178-86. [PubMed: 17709533]  [MGI Ref ID J:151828]

Fang C; Miwa T; Song WC. 2011. Decay-accelerating factor regulates T-cell immunity in the context of inflammation by influencing costimulatory molecule expression on antigen-presenting cells. Blood 118(4):1008-14. [PubMed: 21652682]  [MGI Ref ID J:174858]

Fang C; Zhang X; Miwa T; Song WC. 2009. Complement promotes the development of inflammatory T-helper 17 cells through synergistic interaction with Toll-like receptor signaling and interleukin-6 production. Blood 114(5):1005-15. [PubMed: 19491392]  [MGI Ref ID J:151430]

Fusakio ME; Mohammed JP; Laumonnier Y; Hoebe K; Kohl J; Mattner J. 2011. C5a regulates NKT and NK cell functions in sepsis. J Immunol 187(11):5805-12. [PubMed: 22058413]  [MGI Ref ID J:179685]

Godau J; Heller T; Hawlisch H; Trappe M; Howells E; Best J; Zwirner J; Verbeek JS; Hogarth PM; Gerard C; Van Rooijen N; Klos A; Gessner JE; Kohl J. 2004. C5a initiates the inflammatory cascade in immune complex peritonitis. J Immunol 173(5):3437-45. [PubMed: 15322209]  [MGI Ref ID J:92709]

Gonzalez JM; Franzke CW; Yang F; Romero R; Girardi G. 2011. Complement activation triggers metalloproteinases release inducing cervical remodeling and preterm birth in mice. Am J Pathol 179(2):838-49. [PubMed: 21801872]  [MGI Ref ID J:174598]

Grumelli S; Lu B; Peterson L; Maeno T; Gerard C. 2011. CD46 Protects against Chronic Obstructive Pulmonary Disease. PLoS One 6(5):e18785. [PubMed: 21573156]  [MGI Ref ID J:172427]

Hawlisch H; Belkaid Y; Baelder R; Hildeman D; Gerard C; Kohl J. 2005. C5a negatively regulates toll-like receptor 4-induced immune responses. Immunity 22(4):415-26. [PubMed: 15845447]  [MGI Ref ID J:97987]

Heimbach L; Li Z; Berkowitz P; Zhao M; Li N; Rubenstein DS; Diaz LA; Liu Z. 2011. The C5a Receptor on Mast Cells Is Critical for the Autoimmune Skin-blistering Disease Bullous Pemphigoid. J Biol Chem 286(17):15003-9. [PubMed: 21393236]  [MGI Ref ID J:172081]

Hopken UE; Lu B; Gerard NP; Gerard C. 1997. Impaired inflammatory responses in the reverse arthus reaction through genetic deletion of the C5a receptor. J Exp Med 186(5):749-56. [PubMed: 9271590]  [MGI Ref ID J:99834]

Issuree PD; Maretzky T; McIlwain DR; Monette S; Qing X; Lang PA; Swendeman SL; Park-Min KH; Binder N; Kalliolias GD; Yarilina A; Horiuchi K; Ivashkiv LB; Mak TW; Salmon JE; Blobel CP. 2013. iRHOM2 is a critical pathogenic mediator of inflammatory arthritis. J Clin Invest :. [PubMed: 23348744]  [MGI Ref ID J:194483]

Kandasamy M; Ying PC; Ho AW; Sumatoh HR; Schlitzer A; Hughes TR; Kemeny DM; Morgan BP; Ginhoux F; Sivasankar B. 2013. Complement mediated signaling on pulmonary CD103(+) dendritic cells is critical for their migratory function in response to influenza infection. PLoS Pathog 9(1):e1003115. [PubMed: 23326231]  [MGI Ref ID J:195879]

Kawikova I; Paliwal V; Szczepanik M; Itakura A; Fukui M; Campos RA; Geba GP; Homer RJ; Iliopoulou BP; Pober JS; Tsuji RF; Askenase PW. 2004. Airway hyper-reactivity mediated by B-1 cell immunoglobulin M antibody generating complement C5a at 1 day post-immunization in a murine hapten model of non-atopic asthma. Immunology 113(2):234-45. [PubMed: 15379984]  [MGI Ref ID J:92933]

Kelly MM; McNagny K; Williams DL; van Rooijen N; Maxwell L; Gwozd C; Mody CH; Kubes P. 2008. The lung responds to zymosan in a unique manner independent of toll-like receptors, complement, and dectin-1. Am J Respir Cell Mol Biol 38(2):227-38. [PubMed: 17717323]  [MGI Ref ID J:146394]

Kerr AR; Paterson GK; Riboldi-Tunnicliffe A; Mitchell TJ. 2005. Innate immune defense against pneumococcal pneumonia requires pulmonary complement component C3. Infect Immun 73(7):4245-52. [PubMed: 15972516]  [MGI Ref ID J:99281]

Kohl J; Baelder R; Lewkowich IP; Pandey MK; Hawlisch H; Wang L; Best J; Herman NS; Sproles AA; Zwirner J; Whitsett JA; Gerard C; Sfyroera G; Lambris JD; Wills-Karp M. 2006. A regulatory role for the C5a anaphylatoxin in type 2 immunity in asthma. J Clin Invest 116(3):783-96. [PubMed: 16511606]  [MGI Ref ID J:106480]

Kumar V; Ali SR; Konrad S; Zwirner J; Verbeek JS; Schmidt RE; Gessner JE. 2006. Cell-derived anaphylatoxins as key mediators of antibody-dependent type II autoimmunity in mice. J Clin Invest 116(2):512-20. [PubMed: 16453025]  [MGI Ref ID J:105421]

Lajoie S; Lewkowich IP; Suzuki Y; Clark JR; Sproles AA; Dienger K; Budelsky AL; Wills-Karp M. 2010. Complement-mediated regulation of the IL-17A axis is a central genetic determinant of the severity of experimental allergic asthma. Nat Immunol 11(10):928-35. [PubMed: 20802484]  [MGI Ref ID J:164688]

Lalli PN; Strainic MG; Yang M; Lin F; Medof ME; Heeger PS. 2008. Locally produced C5a binds to T cell-expressed C5aR to enhance effector T-cell expansion by limiting antigen-induced apoptosis. Blood 112(5):1759-66. [PubMed: 18567839]  [MGI Ref ID J:138718]

Lee HM; Wu W; Wysoczynski M; Liu R; Zuba-Surma EK; Kucia M; Ratajczak J; Ratajczak MZ. 2009. Impaired mobilization of hematopoietic stem/progenitor cells in C5-deficient mice supports the pivotal involvement of innate immunity in this process and reveals novel promobilization effects of granulocytes. Leukemia 23(11):2052-62. [PubMed: 19657368]  [MGI Ref ID J:154482]

Li Q; Peng Q; Xing G; Li K; Wang N; Farrar CA; Meader L; Sacks SH; Zhou W. 2010. Deficiency of C5aR prolongs renal allograft survival. J Am Soc Nephrol 21(8):1344-53. [PubMed: 20651167]  [MGI Ref ID J:185916]

Liang S; Krauss JL; Domon H; McIntosh ML; Hosur KB; Qu H; Li F; Tzekou A; Lambris JD; Hajishengallis G. 2011. The C5a receptor impairs IL-12-dependent clearance of Porphyromonas gingivalis and is required for induction of periodontal bone loss. J Immunol 186(2):869-77. [PubMed: 21149611]  [MGI Ref ID J:168772]

Liu J; Lin F; Strainic MG; An F; Miller RH; Altuntas CZ; Heeger PS; Tuohy VK; Medof ME. 2008. IFN-{gamma} and IL-17 Production in Experimental Autoimmune Encephalomyelitis Depends on Local APC-T Cell Complement Production. J Immunol 180(9):5882-9. [PubMed: 18424707]  [MGI Ref ID J:134318]

Looney MR; Su X; Van Ziffle JA; Lowell CA; Matthay MA. 2006. Neutrophils and their Fc gamma receptors are essential in a mouse model of transfusion-related acute lung injury. J Clin Invest 116(6):1615-23. [PubMed: 16710475]  [MGI Ref ID J:110368]

Markiewski MM; DeAngelis RA; Benencia F; Ricklin-Lichtsteiner SK; Koutoulaki A; Gerard C; Coukos G; Lambris JD. 2008. Modulation of the antitumor immune response by complement. Nat Immunol 9(11):1225-35. [PubMed: 18820683]  [MGI Ref ID J:143335]

Markiewski MM; DeAngelis RA; Strey CW; Foukas PG; Gerard C; Gerard N; Wetsel RA; Lambris JD. 2009. The regulation of liver cell survival by complement. J Immunol 182(9):5412-8. [PubMed: 19380788]  [MGI Ref ID J:147949]

Mehlhop E; Diamond MS. 2006. Protective immune responses against West Nile virus are primed by distinct complement activation pathways. J Exp Med 203(5):1371-81. [PubMed: 16651386]  [MGI Ref ID J:124138]

Miwa T; Sato S; Gullipalli D; Nangaku M; Song WC. 2013. Blocking Properdin, the Alternative Pathway, and Anaphylatoxin Receptors Ameliorates Renal Ischemia-Reperfusion Injury in Decay-Accelerating Factor and CD59 Double-Knockout Mice. J Immunol 190(7):3552-9. [PubMed: 23427256]  [MGI Ref ID J:194520]

Mullaly SC; Kubes P. 2006. The role of TLR2 in vivo following challenge with Staphylococcus aureus and prototypic ligands. J Immunol 177(11):8154-63. [PubMed: 17114491]  [MGI Ref ID J:140680]

Mullick A; Tremblay J; Leon Z; Gros P. 2011. A novel role for the fifth component of complement (C5) in cardiac physiology. PLoS One 6(8):e22919. [PubMed: 21829669]  [MGI Ref ID J:176526]

Nigrovic PA; Binstadt BA; Monach PA; Johnsen A; Gurish M; Iwakura Y; Benoist C; Mathis D; Lee DM. 2007. Mast cells contribute to initiation of autoantibody-mediated arthritis via IL-1. Proc Natl Acad Sci U S A 104(7):2325-30. [PubMed: 17277081]  [MGI Ref ID J:119744]

Norman MU; Hulliger S; Colarusso P; Kubes P. 2008. Multichannel fluorescence spinning disk microscopy reveals early endogenous CD4 T cell recruitment in contact sensitivity via complement. J Immunol 180(1):510-21. [PubMed: 18097053]  [MGI Ref ID J:130912]

Nozaki M; Raisler BJ; Sakurai E; Sarma JV; Barnum SR; Lambris JD; Chen Y; Zhang K; Ambati BK; Baffi JZ; Ambati J. 2006. Drusen complement components C3a and C5a promote choroidal neovascularization. Proc Natl Acad Sci U S A 103(7):2328-33. [PubMed: 16452172]  [MGI Ref ID J:106063]

Okinaga S; Slattery D; Humbles A; Zsengeller Z; Morteau O; Kinrade MB; Brodbeck RM; Krause JE; Choe HR; Gerard NP; Gerard C. 2003. C5L2, a nonsignaling C5A binding protein. Biochemistry 42(31):9406-15. [PubMed: 12899627]  [MGI Ref ID J:115691]

Osaka H; McGinty A; Hoepken UE; Lu B; Gerard C; Pasinetti GM. 1999. Expression of C5a receptor in mouse brain: role in signal transduction and neurodegeneration. Neuroscience 88(4):1073-82. [PubMed: 10336122]  [MGI Ref ID J:52135]

Peng Q; Li K; Wang N; Li Q; Asgari E; Lu B; Woodruff TM; Sacks SH; Zhou W. 2009. Dendritic cell function in allostimulation is modulated by C5aR signaling. J Immunol 183(10):6058-68. [PubMed: 19864610]  [MGI Ref ID J:157171]

Qing X; Koo GC; Salmon JE. 2012. Complement regulates conventional DC-mediated NK-cell activation by inducing TGF-beta1 in Gr-1+ myeloid cells. Eur J Immunol 42(7):1723-34. [PubMed: 22535677]  [MGI Ref ID J:187776]

Ramos TN; Darley MM; Hu X; Billker O; Rayner JC; Ahras M; Wohler JE; Barnum SR. 2011. Cutting edge: the membrane attack complex of complement is required for the development of murine experimental cerebral malaria. J Immunol 186(12):6657-60. [PubMed: 21572031]  [MGI Ref ID J:175481]

Ramos TN; Wohler JE; Barnum SR. 2009. Deletion of both the C3a and C5a receptors fails to protect against experimental autoimmune encephalomyelitis. Neurosci Lett 467(3):234-6. [PubMed: 19850104]  [MGI Ref ID J:156903]

Redecha P; Tilley R; Tencati M; Salmon JE; Kirchhofer D; Mackman N; Girardi G. 2007. Tissue factor: a link between C5a and neutrophil activation in antiphospholipid antibody induced fetal injury. Blood 110(7):2423-31. [PubMed: 17536017]  [MGI Ref ID J:147022]

Reiman R; Gerard C; Campbell IL; Barnum SR. 2002. Disruption of the C5a receptor gene fails to protect against experimental allergic encephalomyelitis. Eur J Immunol 32(4):1157-63. [PubMed: 11932923]  [MGI Ref ID J:75976]

Rittirsch D; Flierl MA; Nadeau BA; Day DE; Huber-Lang M; Mackay CR; Zetoune FS; Gerard NP; Cianflone K; Kohl J; Gerard C; Sarma JV; Ward PA. 2008. Functional roles for C5a receptors in sepsis. Nat Med 14(5):551-7. [PubMed: 18454156]  [MGI Ref ID J:136703]

Sadik CD; Kim ND; Iwakura Y; Luster AD. 2012. Neutrophils orchestrate their own recruitment in murine arthritis through C5aR and FcgammaR signaling. Proc Natl Acad Sci U S A 109(46):E3177-85. [PubMed: 23112187]  [MGI Ref ID J:191734]

Sakuma M; Morooka T; Wang Y; Shi C; Croce K; Gao H; Strainic M; Medof ME; Simon DI. 2010. The intrinsic complement regulator decay-accelerating factor modulates the biological response to vascular injury. Arterioscler Thromb Vasc Biol 30(6):1196-202. [PubMed: 20299685]  [MGI Ref ID J:180868]

Scholzen TE; Steinhoff M; Sindrilaru A; Schwarz A; Bunnett NW; Luger TA; Armstrong CA; Ansel JC. 2004. Cutaneous allergic contact dermatitis responses are diminished in mice deficient in neurokinin 1 receptors and augmented by neurokinin 2 receptor blockage. FASEB J 18(9):1007-9. [PubMed: 15084523]  [MGI Ref ID J:118457]

Seshan SV; Franzke CW; Redecha P; Monestier M; Mackman N; Girardi G. 2009. Role of tissue factor in a mouse model of thrombotic microangiopathy induced by antiphospholipid antibodies. Blood 114(8):1675-83. [PubMed: 19535796]  [MGI Ref ID J:152265]

Skokowa J; Ali SR; Felda O; Kumar V; Konrad S; Shushakova N; Schmidt RE; Piekorz RP; Nurnberg B; Spicher K; Birnbaumer L; Zwirner J; Claassens JW; Verbeek JS; van Rooijen N; Kohl J; Gessner JE. 2005. Macrophages induce the inflammatory response in the pulmonary Arthus reaction through G alpha i2 activation that controls C5aR and Fc receptor cooperation. J Immunol 174(5):3041-50. [PubMed: 15728518]  [MGI Ref ID J:97718]

Strainic MG; Liu J; Huang D; An F; Lalli PN; Muqim N; Shapiro VS; Dubyak GR; Heeger PS; Medof ME. 2008. Locally produced complement fragments C5a and C3a provide both costimulatory and survival signals to naive CD4+ T cells. Immunity 28(3):425-35. [PubMed: 18328742]  [MGI Ref ID J:132942]

Strainic MG; Shevach EM; An F; Lin F; Medof ME. 2012. Absence of signaling into CD4(+) cells via C3aR and C5aR enables autoinductive TGF-beta1 signaling and induction of Foxp3(+) regulatory T cells. Nat Immunol 14(2):162-71. [PubMed: 23263555]  [MGI Ref ID J:192613]

Strait RT; Hicks W; Barasa N; Mahler A; Khodoun M; Kohl J; Stringer K; Witte D; Van Rooijen N; Susskind BM; Finkelman FD. 2011. MHC class I-specific antibody binding to nonhematopoietic cells drives complement activation to induce transfusion-related acute lung injury in mice. J Exp Med 208(12):2525-44. [PubMed: 22025304]  [MGI Ref ID J:178758]

Tsuji RF; Kawikova I; Ramabhadran R; Akahira-Azuma M; Taub D; Hugli TE; Gerard C; Askenase PW. 2000. Early local generation of C5a initiates the elicitation of contact sensitivity by leading to early T cell recruitment. J Immunol 165(3):1588-98. [PubMed: 10903768]  [MGI Ref ID J:120437]

Tu Z; Bu H; Dennis JE; Lin F. 2010. Efficient osteoclast differentiation requires local complement activation. Blood 116(22):4456-63. [PubMed: 20709903]  [MGI Ref ID J:166640]

Veninga H; Hoek RM; de Vos AF; de Bruin AM; An FQ; van der Poll T; van Lier RA; Medof ME; Hamann J. 2011. A novel role for CD55 in granulocyte homeostasis and anti-bacterial host defense. PLoS One 6(10):e24431. [PubMed: 21984892]  [MGI Ref ID J:178122]

Vieyra M; Leisman S; Raedler H; Kwan WH; Yang M; Strainic MG; Medof ME; Heeger PS. 2011. Complement regulates CD4 T-cell help to CD8 T cells required for murine allograft rejection. Am J Pathol 179(2):766-74. [PubMed: 21704012]  [MGI Ref ID J:174402]

Wang X; Werneck MB; Wilson BG; Kim HJ; Kluk MJ; Thom CS; Wischhusen JW; Evans JA; Jesneck JL; Nguyen P; Sansam CG; Cantor H; Roberts CW. 2011. TCR-dependent transformation of mature memory phenotype T cells in mice. J Clin Invest 121(10):3834-45. [PubMed: 21926465]  [MGI Ref ID J:178492]

Welch TR; Frenzke M; Witte D; Davis AE. 2002. C5a is important in the tubulointerstitial component of experimental immune complex glomerulonephritis. Clin Exp Immunol 130(1):43-8. [PubMed: 12296852]  [MGI Ref ID J:79194]

Wiege K; Le DD; Syed SN; Ali SR; Novakovic A; Beer-Hammer S; Piekorz RP; Schmidt RE; Nurnberg B; Gessner JE. 2012. Defective macrophage migration in Galphai2- but not Galphai3-deficient mice. J Immunol 189(2):980-7. [PubMed: 22706085]  [MGI Ref ID J:189540]

Zhang X; Kimura Y; Fang C; Zhou L; Sfyroera G; Lambris JD; Wetsel RA; Miwa T; Song WC. 2007. Regulation of Toll-like receptor-mediated inflammatory response by complement in vivo. Blood 110(1):228-36. [PubMed: 17363730]  [MGI Ref ID J:145413]

Zhang X; Lewkowich IP; Kohl G; Clark JR; Wills-Karp M; Kohl J. 2009. A protective role for C5a in the development of allergic asthma associated with altered levels of B7-H1 and B7-DC on plasmacytoid dendritic cells. J Immunol 182(8):5123-30. [PubMed: 19342693]  [MGI Ref ID J:147487]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX12

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, these mice may be bred as homozygotes. For homozygous colonies, SPF conditions are recommended as mutant mice have impaired clearance of bacterial infection.
Mating System	Homozygote x Homozygote         (Female x Male)   03-JAN-08
Diet Information	LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

Control Information

	Control
	000651 BALB/cJ
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

See Terms of Use tab for General Terms and Conditions

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering Information
JAX^® Mice
Surgical and Preconditioning Services
JAX^® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.