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Strain Name:

B6;129P2-Mecp2tm1Bird/J

Stock Number:

006847

Availability:

Under Development for Distribution Colony

To register your interest in this strain go to the Strain Interest Form.

General Terms and Conditions

Genes & Alleles   Mecp2;   Mecp2tm1Bird;


Product Information

Strain Details

Type JAX® GEMM® Strain - Mutant Stock
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Targeted Mutation
Mating SystemHomozygote x Hemizygote         (Female x Male)
Specieslaboratory mouse
Donating Investigator Adrian Bird,   University of Edinburgh
Generation?+F1 (09-JAN-08)

Strain Description
These mice possess two functional loxP sites flanking exons 3-4 of the targeted gene on the X chromosome. Homozygous females and hemizygous males are viable and fertile. Northern blot analysis showed the expected mature transcript from the Mecp2lox locus. Also detected was an unspliced beta-globin transcript that was introduced into the locus as part of the targeting vector. When these mutant mice are bred to mice that express cre recombinase, resulting offspring will have exons 3-4 deleted in the cre-expressing tissue(s). Mice with this X-linked floxed mutation may be useful in neurological and developmental studies of Rett syndrome.

For example, when crossed to a strain expressing Cre recombinase in nervous tissue (see Stock No. 003771), this mutant mouse strain develops a neurological phenotype that mimics Rett syndrome.

Strain Development
A targeting vector was designed to insert a loxP site upstream of exon 3, as well as a human beta-globin intron 2 and polyadenylation signal followed by a loxp-flanked TK-neo cassette all downstream of the stop codon in exon 4, of the targeted gene. The donating investigator reports that the "middle" loxP site (just 5' of the TK-neo cassette) is non-functional; thus only the loxP sites upstream of exon 3 and downstream of the TK-neo cassette are functional. The construct was electroporated into 129P2/OlaHsd-derived E14TG2a embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts, and chimeric offspring were bred to C57BL/6 mice. Heterozygous females (with exons 3-4 flanked by functional loxP sites) were mated with wildtype C57BL/6 animals. The resulting hemizygous males were bred with heterozygous females to generate homozygous females. Homozygous females and hemizygous males were bred together for many generations prior to arrival at The Jackson Laboratory.

Related Disease (OMIM) Terms

Rett Syndrome; RTT
Mammalian Phenotype Terms assigned by genotype

The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.

Mecp2tm1Bird/Y Tg(Nes-cre)1Kln/0

        involves: 129P2/OlaHsd * C57BL/6 * SJL   (conditional)
  • life span-post-weaning/aging
  • premature death (MGI Ref ID J:67910)
  • behavior/neurological phenotype
  • abnormal gait (MGI Ref ID J:67910)
    • develop a stiff, uncoordinated gait
  • hypoactivity (MGI Ref ID J:67910)
  • limb grasping (MGI Ref ID J:67910)
  • craniofacial phenotype
  • abnormal tooth morphology (MGI Ref ID J:67910)
    • frequently exhibit uneven wearing of the teeth
  • endocrine/exocrine gland phenotype
  • cryptorchism (MGI Ref ID J:67910)
  • growth/size phenotype
  • decreased body weight (MGI Ref ID J:67910)
  • reproductive system phenotype
  • cryptorchism (MGI Ref ID J:67910)

Gene & Allele Details

Allele Symbol Mecp2tm1Bird
Allele Name targeted mutation 1, Adrian Bird
Common Name(s) Mecp2lox;
Mutation Made By Adrian Bird,   University of Edinburgh
Strain of Origin129P2/OlaHsd
ES Cell Line NameE14TG2a
ES Cell Line Strain129P2/OlaHsd
Gene Symbol and Name Mecp2, methyl CpG binding protein 2
Chromosome X
Gene Common Name(s) 1500041B07Rik; AUTSX3; BB130002; D630021H01Rik; DKFZp686A24160; MRX16; MRX79; MRXS13; MRXSL; Mbd5; PPMX; RIKEN cDNA 1500041B07 gene; RIKEN cDNA D630021H01 gene; RTS; RTT; WBP10; expressed sequence BB130002;
Molecular Note Insertion of a neomycin resistance cassette into the gene introduced loxP sites that flank exons 3 and 4, and added an intron and polyadenylation signal from the human beta globin gene. From the mutated allele, Northern blot analysis detected the wild type mature transcript and also a transcript in which the beta globin intron was unspliced. [MGI Ref ID J:67910]

Control Information

  Control
   100492 B6129PF1/J (approximate)
   100903 B6129PF2/J (approximate)
 
  Considerations for Choosing Controls

Genotyping Protocols

Mecp2tm1Bird

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, females homozygous for this X-linked mutation can be bred with males hemizygous for this X-linked mutation.
Diet Information LabDiet® 5K52/5K67

Related Strains

Strains carrying other alleles of Mecp2
003890   B6.129P2(C)-Mecp2tm1.1Bird/J
005439   B6.129S-Mecp2tm1Hzo/J
006849   B6;129P2-Mecp2tm2Bird/J
View Strains carrying other alleles of Mecp2     (3 strains)

Research Applications

This mouse can be used to support research in many areas including:

Mouse/Human Gene Homologs
Rett syndrome

Neurobiology Research
Behavioral and Learning Defects
Cre-lox System (loxP-flanked Sequences)
Neurodevelopmental Defects (Rett's syndrome)

Research Tools
Cre-lox System (loxP-flanked Sequences)
Developmental Biology Research (Cre-lox System)

Mecp2tm1Bird related

Neurobiology Research
Ataxia (Movement) Defects

References

Selected Reference(s)

Guy J; Hendrich B; Holmes M; Martin JE; Bird A. 2001. A mouse Mecp2-null mutation causes neurological symptoms that mimic Rett syndrome. Nat Genet 27(3):322-6. [PubMed: 11242117]  [MGI Ref ID J:67910]

Additional References

Price and Supply Information

Strain Name: B6;129P2-Mecp2tm1Bird/J
Stock Number: 006847
 

This strain is currently Under Development for Distribution Colony.
To register your interest in this strain go to the Strain Interest Form.

Estimated Available for Sale Date:
Can not yet predict an estimated Available for Sale date; Colony breeding poorly.

Please note: Estimated available for sale dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for sale depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain/interest registered.

View All Strains Under Development

Supply Details

Standard SupplyUnder Development for Distribution Colony
Supply Notes This strain is included in the Induced Mutant Resource Colony collection.
LicensingSee General Terms and Conditions below for Licensing and Use Restrictions  
Control InformationView Control Information in Strain Details.

General Terms and Conditions

View JAX® Mice & Services Conditions of Use.

Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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