Strain Name: |
B6;129P2-Mecp2tm2Bird/J |
|---|---|
Stock Number: |
006849 |
Availability: | Repository- Live |
General Terms and Conditions |
| Former Name |
B6.129P2-Mecp2tm2Bird/J (Changed: 06-MAY-08
) |
| Genes & Alleles | Mecp2; Mecp2tm2Bird; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Targeted Mutation Mating System Hemizygote x +/+ sibling (Female x Male) Species laboratory mouse Donating Investigator Adrian Bird, University of Edinburgh Generation N6+N1 (14-NOV-07) Strain Description
These mice possess a loxP-flanked STOP cassette in intron 2 of the targeted gene on the X chromosome. Western blot and hybridization analysis confirm the absence of wildtype protein from the targeted allele. Hemizygous (Mecp2lox-Stop/y) males do not breed and develop Rett syndrome symptoms (reduced mobility, hindlimb clasping) at approximately 6 weeks of age, with death occurring at approximately 11 weeks of age. Heterozygous females are fertile until developing Rett syndrome characteristics at 4-12 months of age. This Rett syndrome-like phenotype is similar to that observed for the traditional knock-out allele (see Stock No. 003890). Cre recombinase-mediated removal of the floxed-STOP cassette restores transcription from the targeted allele and MECP2 protein activity to normal, and reverses the Rett syndrome-like neurological defects.This mutant mouse strain may be bred to a strain expressing tamoxifen inducible Cre recombinase in most tissues (see Stock No. 004682).
Mice with this X-linked lox-STOP mutation may be useful in neurological and developmental studies of Rett syndrome and its amelioration upon excision of the lox-STOP cassette.
Strain Development
A targeting vector was designed to insert a loxP-flanked STOP-Neo cassette into intron 2 of the targeted gene. The STOP-Neo cassette is composed of a PGK-Neo cassette followed by the 3' portion of the yeast His3 gene, an SV40 polyadenylation sequence, and a false translation initiation codon followed by a 5' splice donor site. The construct was electroporated into 129P2/OlaHsd-derived E14TG2a embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts, and chimeric offspring were bred to C57BL/6 mice. Heterozygous female mutants were bred to wildtype C57BL/6 males for 5 generations prior to arrival at The Jackson Laboratory. A SNP (single nucleotide polymorphism) panel analysis performed by The Jackson Laboratory revealed that this strain is on a mixed B6;129 genetic background background.
Related Disease (OMIM) Terms |
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Mecp2tm2Bird | ||
|---|---|---|---|
| Allele Name | targeted mutation 2, Adrian Bird | ||
| Common Name(s) | Mecp2lox-stop; | ||
| Mutation Made By | Adrian Bird, University of Edinburgh | ||
| Strain of Origin | 129P2/OlaHsd | ||
| ES Cell Line Name | E14TG2a | ||
| ES Cell Line Strain | 129P2/OlaHsd | ||
| Gene Symbol and Name | Mecp2, methyl CpG binding protein 2 | ||
| Chromosome | X | ||
| Gene Common Name(s) | 1500041B07Rik; AUTSX3; BB130002; D630021H01Rik; DKFZp686A24160; MRX16; MRX79; MRXS13; MRXSL; Mbd5; PPMX; RIKEN cDNA 1500041B07 gene; RIKEN cDNA D630021H01 gene; RTS; RTT; WBP10; expressed sequence BB130002; | ||
| Molecular Note | Cre recombinase-reversible gene inactivation was accomplished by insertion into intron 2 of a 3.1-kb DNA fragment containing a loxP-flanked "neostop" cassette, which comprises a neomycin resistance cassette followed by a transcriptional/translational "stop" cassette composed of 550 bp of 3' sequence from the Saccharomyces cerevisiae His3 gene, an SV40 polyadenylation sequence, and a synthetic sequence containing a false translation initiation codon (ATG) immediately followed by a consensus splice donor sequence. No protein product from the endogenous gene was detected by western blot or immunofluorescence analysis of brains of mutant mice. [MGI Ref ID J:111323] [MGI Ref ID J:118365] [MGI Ref ID J:75205] | ||
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 100492 B6129PF1/J | (approximate) | |
| Considerations for Choosing Controls | ||
Genotyping Protocols
Mecp2tm2Bird
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, females hemizygous for this X-linked mutation can be bred with wildtype siblings. The donating investigator recommends replacing hemizygous female breeders when Rett syndrome symptoms appear or when females fail to produce or care for regular litters (may be as early as 4-6 months. The donating investigator also reports that breeding performance may be improved if mice are maintained on a mixed C57BL/6;BALB/c background. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying other alleles of Mecp2
003890 B6.129P2(C)-Mecp2tm1.1Bird/J 005439 B6.129S-Mecp2tm1Hzo/J 006847 B6;129P2-Mecp2tm1Bird/J View Strains carrying other alleles of Mecp2 (3 strains)
Additional Web Information
Congenic Nomenclature
Cre-lox or FLP-FRT Systems
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
Mecp2tm2Bird relatedMouse/Human Gene Homologs
Rett syndrome
Neurobiology Research
Behavioral and Learning Defects
Cre-lox System (loxP-flanked Sequences)
Neurodevelopmental Defects (Rett's syndrome)
Research Tools
Cre-lox System (loxP-flanked Sequences)
Developmental Biology Research (Cre-lox System)
Neurobiology Research
Ataxia (Movement) Defects
References
Selected Reference(s)
Additional ReferencesGuy J; Gan J; Selfridge J; Cobb S; Bird A. 2007. Reversal of neurological defects in a mouse model of Rett syndrome. Science 315(5815):1143-7. [PubMed: 17289941] [MGI Ref ID J:118365]
Price and Supply Information
| Strain Name: | B6;129P2-Mecp2tm2Bird/J |
| Stock Number: | 006849 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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