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- Description
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- Genes & alleles
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Description
Strain Information
Former Names STOCK Tg(ACTB-Bgeo,-NOTCH1,-EGFP)1Lbe/J (Changed: 08-MAY-08 ) STOCK Tg(ACTB-Bgeo,NOTCH1,EGFP)1Lbe/J (Changed: 19-APR-07 ) Type Mutant Stock; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System +/+ sibling x Hemizygote (Female x Male) 01-MAY-08 Species laboratory mouse Generation ?+N2F2 (30-APR-08) Donating Investigator Corrinne Lobe, Sunnybrook & Women's College HSC Description
Mice hemizygous for this Cre-conditional IC-Notch (or Z/EG-Notch) transgene are viable and fertile. Homozygotes die in utero, presumably due to high lacZ expression. Prior to Cre-mediated excision of the "floxed" STOP sequence, high expression of lacZ is observed in cells and tissues. When bred to Cre recombinase expressing mice, the STOP sequence (and beta-geo) is removed in the resulting offspring, allowing transcription/co-expression of both the intracellular human NOTCH1 (IC-Notch) and EGFP to proceed in the Cre recombinase expressing cells. For example, endothelial expression of IC-Notch using different Cre-transgenic matings is associated with neural, somite and angiogenic defects, infertility in females, and embryonic lethality in the resulting offspring. These transgenic mice may be useful for global expression of lacZ or, when crossed to a Cre recombinase expressing strain, for studying the role of Notch signaling during both embryonic development and adulthood.Development
A transgenic construct was designed with the CMV enhancer/chicken beta-actin promoter, loxP-flanked STOP sequence (beta-geo reporter followed by SV40 polyadenylation signal), Myc-tagged intracellular portion of human NOTCH1 (IC-Notch), internal ribosomal entry site, and an enhanced green fluorescent protein (EGFP) gene. This construct was electroporated into 129X1/SvJ x 129S1/Sv-derived R1 embryonic stem (ES) cells. ES cells with strong lacZ expression, single copy transgene integration, and efficient expression of EGFP following Cre-mediated excision of the STOP sequence were selected and aggregated with E2.5 8-cell CD1 mouse embryos. Following transfer into pseudo-pregnant foster mothers, chimeric males were mated to wild-type CD1 females to establish founder mice. This strain was maintained by breeding hemizygous mice to CD1 or wild-type siblings for approximately seven generations prior to arrival at The Jackson Laboratory. Upon arrival, these mice were bred at least one generation to C57BL/6J.
Control Information
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| Noncarrier | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (225 strains)
006481 C57BL/6J-Tg(ACTB-NOTCH1)1Shn/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Bgeo relatedDevelopmental Biology Research
Embryonic Lethality (Homozygous)
Research Tools
lacZ Expression
Cre-lox System
loxP-flanked Sequences
Developmental Biology Research
Cre-lox System
Fluorescent Proteins
Genetics Research
Tissue/Cell Markers: Cre-lox System
GFP relatedResearch Tools
lacZ Expression
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(CAG-Bgeo,-NOTCH1,-EGFP)1Lbe | ||
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| Allele Name | transgene insertion 1, Corinne G Lobe | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | IC-Notch1; Tg(ACTB-Bgeo,-NOTCH1,-EGFP)1Lbe; Tg(CAG-Bgeo,-NOTCH1,-EGFP)1Lbe; Z/EG-Notch; | ||
| Mutation Made By | Corrinne Lobe, Sunnybrook & Women's College HSC | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| ES Cell Line Name | R1 | ||
| ES Cell Line Strain | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Site of Expression | lacZ is highly expressed in most cells and tissues. When bred to Cre recombinase expressing mice, lacZ expression is replaced with co-expression of intracellular human NOTCH1 (IC-Notch) and EGFP in Cre recombinase expressing cells. | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Expressed Gene | Bgeo, fusion of beta-galactosidase and neomycin phosphotransferase genes , E. coli | ||
| Expressed Gene | NOTCH1, Notch homolog 1, translocation-associated (Drosophila), human | ||
| Promoter | ACTB, actin, beta, chicken | ||
| Molecular Note | A transgenic construct was designed with the CMV enhancer/chicken beta-actin promoter, loxP-flanked STOP sequence (beta-geo reporter followed by SV40 polyadenylation signal), Myc-tagged intracellular portion of human NOTCH1 (IC-Notch), internal ribosomalentry site, and an enhanced green fluorescent protein (EGFP) gene. Prior to cre-mediated excision of the "floxed" STOP sequence, high expression of lacZ is observed in cells and tissues. When bred to Cre recombinase expressing mice, the STOP sequence (and beta-geo) is removed in the resulting offspring, allowing transcription/co-expression of both the intracellular human NOTCH1 (IC-Notch) and EGFP to proceed in the Cre recombinase expressing cells and enabling tissue-specific studies of NOTCH expression using appropriate Cre mice. [MGI Ref ID J:119129] [MGI Ref ID J:83045] | ||
| Gene Symbol and Name | Tg(CAG-Bgeo,-NOTCH1,-EGFP)1Lbe, transgene insertion 1, Corinne G Lobe | ||
| Chromosome | UN | ||
| Gene Common Name(s) | IC-Notch1; Tg(ACTB-Bgeo,-NOTCH1,-EGFP)1Lbe; Z/EG-Notch; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Fluorescent Proteins (Generic GFP), Melt Curve Analysis
Fluorescent Proteins (Generic GFP), Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Liu J; Lobe CG. 2007. Cre-conditional expression of constitutively active Notch1 in transgenic mice. Genesis 45(5):259-65. [PubMed: 17440974] [MGI Ref ID J:121805]
Additional References
Tg(CAG-Bgeo,-NOTCH1,-EGFP)1Lbe relatedLiu J; Lobe CG. 2007. Constitutive notch signaling in adult transgenic mice inhibits bFGF-induced angiogenesis and blocks ovarian follicle development MGI Direct Data Submission :. [MGI Ref ID J:119129]
Novak A; Guo C; Yang W; Nagy A; Lobe CG. 2000. Z/EG, a double reporter mouse line that expresses enhanced green fluorescent protein upon Cre-mediated excision. Genesis 28(3-4):147-55. [PubMed: 11105057] [MGI Ref ID J:83045]
Waters AM; Wu MY; Onay T; Scutaru J; Liu J; Lobe CG; Quaggin SE; Piscione TD. 2008. Ectopic notch activation in developing podocytes causes glomerulosclerosis. J Am Soc Nephrol 19(6):1139-57. [PubMed: 18337488] [MGI Ref ID J:149922]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, hemizygotes are bred to wildtype siblings. Homozygous mice die in utero presumably due to high lacZ expression. The donating investigator recommends genotyping animals by lacZ staining of ear notches.
Mating System +/+ sibling x Hemizygote (Female x Male) 01-MAY-08 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $243.50 Female or Male Hemizygous for Tg(CAG-Bgeo,-NOTCH1,-EGFP)1Lbe
Pairs /Price (US dollars $) Pair Genotype $297.85 Hemizygous for Tg(CAG-Bgeo,-NOTCH1,-EGFP)1Lbe x Noncarrier $297.85 Noncarrier x Hemizygous for Tg(CAG-Bgeo,-NOTCH1,-EGFP)1Lbe
Additional Supply Details
| Pricing for International shipping destinations |
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Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $316.60 Female or Male Hemizygous for Tg(CAG-Bgeo,-NOTCH1,-EGFP)1Lbe
Pairs /Price (US dollars $) Pair Genotype $387.30 Hemizygous for Tg(CAG-Bgeo,-NOTCH1,-EGFP)1Lbe x Noncarrier $387.30 Noncarrier x Hemizygous for Tg(CAG-Bgeo,-NOTCH1,-EGFP)1Lbe
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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