Strain Name: |
STOCK Tg(CAG-Bgeo,-NOTCH1,-EGFP)1Lbe/J |
|---|---|
Stock Number: |
006850 |
Availability: | Repository- Live |
General Terms and Conditions |
| Former Name |
STOCK Tg(ACTB-Bgeo,-NOTCH1,-EGFP)1Lbe/J (Changed: 08-MAY-08
) |
|
STOCK Tg(ACTB-Bgeo,NOTCH1,EGFP)1Lbe/J (Changed: 19-APR-07
) | |
| Genes & Alleles | ACTB; Bgeo; GFP; NOTCH1; Tg(CAG-Bgeo,-NOTCH1,-EGFP)1Lbe; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Mating System +/+ sibling x Hemizygote (Female x Male) Species laboratory mouse Donating Investigator Corrinne Lobe, Sunnybrook & Women's College HSC Generation ?+N2F2 (30-APR-08) Strain Description
Mice hemizygous for this Cre-conditional IC-Notch (or Z/EG-Notch) transgene are viable and fertile. Homozygotes die in utero, presumably due to high lacZ expression. Prior to Cre-mediated excision of the "floxed" STOP sequence, high expression of lacZ is observed in cells and tissues. When bred to Cre recombinase expressing mice, the STOP sequence (and beta-geo) is removed in the resulting offspring, allowing transcription/co-expression of both the intracellular human NOTCH1 (IC-Notch) and EGFP to proceed in the Cre recombinase expressing cells. For example, endothelial expression of IC-Notch using different Cre-transgenic matings is associated with neural, somite and angiogenic defects, infertility in females, and embryonic lethality in the resulting offspring. These transgenic mice may be useful for global expression of lacZ or, when crossed to a Cre recombinase expressing strain, for studying the role of Notch signaling during both embryonic development and adulthood.Strain Development
A transgenic construct was designed with the CMV enhancer/chicken beta-actin promoter, loxP-flanked STOP sequence (beta-geo reporter followed by SV40 polyadenylation signal), Myc-tagged intracellular portion of human NOTCH1 (IC-Notch), internal ribosomal entry site, and an enhanced green fluorescent protein (EGFP) gene. This construct was electroporated into 129X1/SvJ x 129S1/Sv-derived R1 embryonic stem (ES) cells. ES cells with strong lacZ expression, single copy transgene integration, and efficient expression of EGFP following Cre-mediated excision of the STOP sequence were selected and aggregated with E2.5 8-cell CD1 mouse embryos. Following transfer into pseudo-pregnant foster mothers, chimeric males were mated to wildtype CD1 females to establish founder mice. This strain was maintained by breeding hemizygous mice to CD1 or wildtype siblings for approximately seven generations prior to arrival at The Jackson Laboratory. Upon arrival, these mice were bred at least one generation to C57BL/6J.
Gene & Allele Details
| Allele Symbol | Tg(CAG-Bgeo,-NOTCH1,-EGFP)1Lbe | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Corinne G Lobe | ||
| Common Name(s) | Tg(ACTB-Bgeo,-NOTCH1,-EGFP)1Lbe; Z/EG-Notch; | ||
| Mutation Made By | Corrinne Lobe, Sunnybrook & Women's College HSC | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| ES Cell Line Name | R1 | ||
| ES Cell Line Strain | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Expressed Gene | NOTCH1, Notch homolog 1, translocation-associated (Drosophila), human | ||
| Expressed Gene | Bgeo, fusion of beta-galactosidase and neomycin phosphotransferase genes , E. coli | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. The original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | ACTB, actin, beta, chicken | ||
| Molecular Note | A transgenic construct was designed with the CMV enhancer/chicken beta-actin promoter, loxP-flanked STOP sequence (beta-geo reporter followed by SV40 polyadenylation signal), Myc-tagged intracellular portion of human NOTCH1 (IC-Notch), internal ribosomalentry site, and an enhanced green fluorescent protein (EGFP) gene. Prior to cre-mediated excision of the "floxed" STOP sequence, high expression of lacZ is observed in cells and tissues. When bred to Cre recombinase expressing mice, the STOP sequence (and beta-geo) is removed in the resulting offspring, allowing transcription/co-expression of both the intracellular human NOTCH1 (IC-Notch) and EGFP to proceed in the Cre recombinase expressing cells and enabling tissue-specific studies of NOTCH expression using appropriate Cre mice. [J:119129] [J:83045] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Fluorescent Proteins
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, hemizygotes are bred to wildtype siblings. Homozygous mice die in utero presumably due to high lacZ expression. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (135 strains)
006481 C57BL/6J-Tg(ACTB-NOTCH1)1Shn/J
Additional Web Information
Cre-lox or FLP-FRT Systems
Fluorescent Proteins/lacZ Systems
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
Bgeo relatedDevelopmental Biology Research
Embryonic Lethality (Homozygous)
Research Tools
Cre-lox-System (loxP-flanked Sequences)
Developmental Biology Research (Cre-lox system)
Fluorescent Proteins
Genetics Research (Tissue/Cell Markers: Cre-lox system)
lacZ Expression
GFP relatedResearch Tools
lacZ Expression
Fluorescent Proteins
References
Selected Reference(s)
Additional ReferencesLiu J; Lobe CG. 2007. Constitutive notch signaling in adult transgenic mice inhibits bFGF-induced angiogenesis and blocks ovarian follicle development MGI Direct Data Submission :. [J:119129]
Price and Supply Information
| Strain Name: | STOCK Tg(CAG-Bgeo,-NOTCH1,-EGFP)1Lbe/J |
| Stock Number: | 006850 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. No age specifications accepted. Colony sized to produce minimal quantities (typically up to 6 mice) upon order receipt; one order per strain. Expected delivery: 1-3 months. Larger quantities or custom orders arranged upon request. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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