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| These mPer1-luc transgenc mice may be useful in studying circadian rhythms and as a real-time reporter of Per1 expression. | |||||||||||||||
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Description
Strain Information
Former Names STOCK Tg(Per1-luc)025Jt/J (Changed: 11-FEB-08 ) Type Congenic; Mutant Stock; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Noncarrier x Hemizygote (Female x Male) 08-AUG-09 Species laboratory mouse Generation N6+ (07-AUG-09) Donating Investigator Joseph Takahashi, Univ Texas Southwestern Medical Ctr Description
Mice hemizygous for the "mPer1-Luc" transgene are viable and fertile, with luciferase (luc) expression driven by the mouse Per1 promoter and 5'-UTR elements. Expression of luc RNA is highly specific to the suprachiasmatic nuclei (SCN) with a clear circadian rhythm that correlates with endogenous Per1. Slice cultures taken from hemizygous mice maintain a circadian rhythm of luminescence for at least 5 days after culturing. A prolonged (6 hour) light pulse treatment during the subjective night rapidly induces both mPer1-luc and endogenous mRNA expression; while the endogenous mRNA level decreased to baseline during the 6 hour period, the mPer1-luc mRNA levels remain higher. These mPer1-luc transgenc mice may be useful in studying circadian rhythms and as a real-time reporter of Per1 expression.In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. As the original publication describes mice on a STOCK genetic background, it should be noted that the phenotype could vary from that originally described. The donating investigator reports that no segregating phenotypic traits are observed on the C57BL/6 congenic background. We will modify the strain description if necessary as published results become available.
Development
This "mPer1-luc" transgene was designed with the firefly luciferase gene (luc) downstream of a 6.7 kbp sequence from the period homolog 1 (Per1) promoter and 5'-UTR sequence. This was engineered such that the Per1 initiator ATG coincided with the luciferase initiator ATG. This construct was microinjected into CD1 mouse embryos. Transgenic offspring (founder line P1L025) were found to have approximately 10 tandem repeats at a single integration site. This line was maintained on the CD1 outbred background. After this, transgenic mice were backcrossed to C57BL/6 for 5 generations prior to arriving at The Jackson Laboratory.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
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Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Neurobiology Research
Circadian Rhythms
Fluorescent protein expression in neural tissue
Research Tools
Fluorescent Proteins
Neurobiology Research
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Per1-luc)025Jt | ||
|---|---|---|---|
| Allele Name | transgene insertion 025, Joseph S Takahashi | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | P1L025; mPer1-luc; | ||
| Mutation Made By | Joseph Takahashi, Univ Texas Southwestern Medical Ctr | ||
| Strain of Origin | CD-1 | ||
| Site of Expression | suprachiasmatic nuclei (SCN) with a clear circadian rhythm | ||
| Expressed Gene | luc, luciferase, firefly | ||
| Promoter | Per1, period homolog 1 (Drosophila), mouse, laboratory | ||
| General Note | 6 transgenic lines were generated and designated by the authors P1L005 (2 copies/genome), P1L025 (1 copy), P1L095 (3), P1L097 (1), P1L128 (2) and P1L141 (10). Brain sections of mice of line Tg(Per1-luc)025Jt (P1L025) were hybridized with luc and Per1 probes to verify the ability of the promoter to maintain a normal circadian pattern of expression after seven days in constant darkness. As results of other experiments were similar for all six lines, data were not differentiated by line. Mice hemizygous for the transgene express luc mRNA in the suprachiasmatic nuclei (SCN) with a circadian rhythm. SCN slice cultures exhibit a circadian rhythm of luminescence for at least 5 days, peaking each day at 6 hours circadian time. Exposure of mice to light for6 hours during early subjective night rapidly induces transcription of both luc and endogenous Per1 in the SCN; however, whereas Per1 mRNA levels return to baseline, luc mRNA levels remain high. | ||
| Molecular Note | A 6,756-base pair DNA fragment containing the putative promoter sequence and the 5'-untranslated region, which encompasses exon 1 and part of exon 2, was ligated to the luciferase gene so that the initiating ATGs of the Per1 and luc genes coincided. [MGI Ref ID J:73700] | ||
| Gene Symbol and Name | Tg(Per1-luc)025Jt, transgene insertion 025, Joseph S Takahashi | ||
| Chromosome | UN | ||
| Gene Common Name(s) | P1L025; mPer1-luc; | ||
Genotyping
Genotyping Information
This strain will not have a genotyping protocol or one is not currently available.
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Wilsbacher LD; Yamazaki S; Herzog ED; Song EJ; Radcliffe LA; Abe M; Block G; Spitznagel E; Menaker M; Takahashi JS. 2002. Photic and circadian expression of luciferase in mPeriod1-luc transgenic mice invivo. Proc Natl Acad Sci U S A 99(1):489-94. [PubMed: 11752392] [MGI Ref ID J:73700]
Additional References
Tg(Per1-luc)025Jt relatedDoyle SE; Yoshikawa T; Hillson H; Menaker M. 2008. Retinal pathways influence temporal niche. Proc Natl Acad Sci U S A 105(35):13133-8. [PubMed: 18695249] [MGI Ref ID J:139704]
Health & husbandry
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, hemizygous mice may be bred together, to wildtype siblings, or to C57BL/6J inbred mice. Mating System Noncarrier x Hemizygote (Female x Male) 08-AUG-09 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
This strain is currently Under Development for Production.
To register your interest in this strain go to the Strain Interest Form.
Estimated Available for Sale Date:
Please note: Estimated available for sale dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for sale depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain/interest registered.
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Supply Details
| Standard Supply | Under Development for Distribution Colony |
|---|---|
| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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