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| These mPer1-luc transgenc mice may be useful in studying circadian rhythms and as a real-time reporter of Per1 expression. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names STOCK Tg(Per1-luc)025Jt/J (Changed: 11-FEB-08 ) Type Congenic; Mutant Stock; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Donating Investigator Dr. Joseph S. Takahashi, Univ Texas Southwestern Medical Ctr Description
Mice hemizygous for the "mPer1-Luc" transgene are viable and fertile, with luciferase (luc) expression driven by the mouse Per1 promoter and 5'-UTR elements. Expression of luc RNA is highly specific to the suprachiasmatic nuclei (SCN) with a clear circadian rhythm that correlates with endogenous Per1. Slice cultures taken from hemizygous mice maintain a circadian rhythm of luminescence for at least 5 days after culturing. A prolonged (6 hour) light pulse treatment during the subjective night rapidly induces both mPer1-luc and endogenous mRNA expression; while the endogenous mRNA level decreased to baseline during the 6 hour period, the mPer1-luc mRNA levels remain higher. These mPer1-luc transgenc mice may be useful in studying circadian rhythms and as a real-time reporter of Per1 expression.In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. As the original publication describes mice on a STOCK genetic background, it should be noted that the phenotype could vary from that originally described. The donating investigator reports that no segregating phenotypic traits are observed on the C57BL/6 congenic background. We will modify the strain description if necessary as published results become available.
Development
This "mPer1-luc" transgene was designed with the firefly luciferase gene (luc) downstream of a 6.7 kbp sequence from the period homolog 1 (Per1) promoter and 5'-UTR sequence. This was engineered such that the Per1 initiator ATG coincided with the luciferase initiator ATG. This construct was microinjected into CD1 mouse embryos. Transgenic offspring (founder line P1L025) were found to have approximately 10 tandem repeats at a single integration site. This line was maintained on the CD1 outbred background. After this, transgenic mice were backcrossed to C57BL/6 for 5 generations prior to arriving at The Jackson Laboratory.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (357 strains)
010831 129S-Per1tm1Drw/J 010491 B6.129-Per1tm1Drw/J 016187 B6.BTBR-Tg(Per1-luc,Per1)1Jt/J 016165 B6.Cg-Tg(Per1-luc)128Jt/J 016166 B6.Cg-Tg(Per1-luc)141Jt/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Neurobiology Research
Circadian Rhythms
Fluorescent protein expression in neural tissue
Research Tools
Fluorescent Proteins
Neurobiology Research
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Per1-luc)025Jt | ||
|---|---|---|---|
| Allele Name | transgene insertion 025, Joseph S Takahashi | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | P1L025; mPer1-luc; mPer1::luciferase; | ||
| Mutation Made By | Dr. Joseph Takahashi, Univ Texas Southwestern Medical Ctr | ||
| Strain of Origin | CD-1 | ||
| Site of Expression | suprachiasmatic nuclei (SCN) with a clear circadian rhythm | ||
| Expressed Gene | luc, luciferase, firefly | ||
| Promoter | Per1, period circadian clock 1, mouse, laboratory | ||
| Gene Symbol and Name | Tg(Per1-luc)025Jt, transgene insertion 025, Joseph S Takahashi | ||
| Chromosome | UN | ||
| Gene Common Name(s) | P1L025; mPer1-luc; | ||
| General Note | 6 transgenic lines were generated and designated by the authors P1L005 (2 copies/genome), P1L025 (1 copy), P1L095 (3), P1L097 (1), P1L128 (2) and P1L141 (10). Brain sections of mice of line Tg(Per1-luc)025Jt (P1L025) were hybridized with luc and Per1 probes to verify the ability of the promoter to maintain a normal circadian pattern of expression after seven days in constant darkness. As results of other experiments were similar for all six lines, data were not differentiated by line. Mice hemizygous for the transgene express luc mRNA in the suprachiasmatic nuclei (SCN) with a circadian rhythm. SCN slice cultures exhibit a circadian rhythm of luminescence for at least 5 days, peaking each day at 6 hours circadian time. Exposure of mice to light for6 hours during early subjective night rapidly induces transcription of both luc and endogenous Per1 in the SCN; however, whereas Per1 mRNA levels return to baseline, luc mRNA levels remain high. | ||
| Molecular Note | A 6,756-base pair DNA fragment containing the putative promoter sequence and the 5'-untranslated region, which encompasses exon 1 and part of exon 2, was ligated to the luciferase gene so that the initiating ATGs of the Per1 and luc genes coincided. [MGI Ref ID J:73700] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(Il2/NFAT-luc)83Rinc, QPCR
Tg(Il2/NFAT-luc)83Rinc, QPCR
Tg(Per1-luc), Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Wilsbacher LD; Yamazaki S; Herzog ED; Song EJ; Radcliffe LA; Abe M; Block G; Spitznagel E; Menaker M; Takahashi JS. 2002. Photic and circadian expression of luciferase in mPeriod1-luc transgenic mice invivo. Proc Natl Acad Sci U S A 99(1):489-94. [PubMed: 11752392] [MGI Ref ID J:73700]
Additional References
Tg(Per1-luc)025Jt relatedDeery MJ; Maywood ES; Chesham JE; Sladek M; Karp NA; Green EW; Charles PD; Reddy AB; Kyriacou CP; Lilley KS; Hastings MH. 2009. Proteomic analysis reveals the role of synaptic vesicle cycling in sustaining the suprachiasmatic circadian clock. Curr Biol 19(23):2031-6. [PubMed: 19913422] [MGI Ref ID J:156786]
Doyle SE; Yoshikawa T; Hillson H; Menaker M. 2008. Retinal pathways influence temporal niche. Proc Natl Acad Sci U S A 105(35):13133-8. [PubMed: 18695249] [MGI Ref ID J:139704]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry When maintaining a live colony, hemizygous mice may be bred together, to wildtype siblings, or to C57BL/6J inbred mice.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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