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| Homozygous mice have normal entrainment and circadian behaviors. These mPer2Luc knock-in mice may be useful as a real-time reporter of circadian dynamics in different tissues. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
Strain Information
Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Homozygote (Female x Male) 12-FEB-08 Species laboratory mouse Generation N11+F13 (18-JUN-12)
Generation DefinitionsDonating Investigator Dr. Joseph S. Takahashi, Univ Texas Southwestern Medical Ctr Description
Mice homozygous for this "mPer2Luc" mutation are viable and fertile with no developmental or morphological differences compared to wildtype littermates. Expression of PERIOD2::LUCIFERASE (or mPER2::LUC) fusion protein during peak periods is similar to endogenous pPER2 patterns. Homozygous mice have normal entrainment and circadian behaviors. These mPer2Luc knock-in mice may be useful as a real-time reporter of circadian dynamics in different tissues.Development
A targeting vector was designed to insert the firefly luciferase (luc) gene (followed by a loxP-flanked neomycin resistance gene) in-frame into exon 23 of the wild-type sequence. The construct was electroporated into 129S6/SvEvTac-derived W4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. Chimeric males were bred to C57BL/6J females. Heterozygotes were backcrossed to C57BL/6J inbred mice for 7-11 generations prior to arrival at The Jackson Laboratory.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (357 strains)
010832 129S-Per2tm1Drw/J 016176 B6(Cg)-Tg(tetO-Per2)2Jt/J 010492 B6.129-Per2tm1Drw/J 003819 B6.Cg-Per2tm1Brd Tyrc-Brd/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested. Advanced Sleep Phase Syndrome, Familial, 1; FASPS1 (PER2)
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Per2tm1Jt/Per2tm1Jt
involves: 129S6/SvEvTac * C57BL/6J
- normal phenotype
- no abnormal phenotype detected
- mice developed normally and showed no overt morphological abnormalities (MGI Ref ID J:89256)
This mouse can be used to support research in many areas including:
Neurobiology Research
Circadian Rhythms
Research Tools
Fluorescent Proteins
Neurobiology Research
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Per2tm1Jt | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Joseph S Takahashi | ||
| Allele Type | Targeted (Reporter) | ||
| Common Name(s) | Per2Luc; mPER2::LUC; mPer2Luc; mPer2Luciferase; | ||
| Mutation Made By | Dr. Joseph Takahashi, Univ Texas Southwestern Medical Ctr | ||
| Strain of Origin | 129S6/SvEvTac | ||
| ES Cell Line Name | W4 | ||
| ES Cell Line Strain | 129S6/SvEvTac | ||
| Site of Expression | Circadian patterns of Per2-Luc fusion protein expression is seen in the following peripheral tissues: SCN, cornea, liver, pituitary, kidney, RCA, lung and tail). | ||
| Expressed Gene | luc, luciferase, firefly | ||
| Molecular Note | A luciferase gene and floxed neo cassette were inserted between exon 23 and the 3' UTR. Mutant transcript was detected in Northern blot of mutant brains and the Per2-Luc fusion protein was detected in Western blots of mutant kidneys. [MGI Ref ID J:89256] | ||
| Gene Symbol and Name | Per2, period circadian clock 2 | ||
| Chromosome | 1 | ||
| Gene Common Name(s) | FASPS; FASPS1; mKIAA0347; mPer2; rPER2; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Per2tm1Jt, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Yoo SH; Yamazaki S; Lowrey PL; Shimomura K; Ko CH; Buhr ED; Siepka SM; Hong HK; Oh WJ; Yoo OJ; Menaker M; Takahashi JS. 2004. PERIOD2::LUCIFERASE real-time reporting of circadian dynamics reveals persistent circadian oscillations in mouse peripheral tissues. Proc Natl Acad Sci U S A 101(15):5339-46. [PubMed: 14963227] [MGI Ref ID J:89256]
Additional References
Per2tm1Jt relatedAkashi M; Hayasaka N; Yamazaki S; Node K. 2008. Mitogen-activated protein kinase is a functional component of the autonomous circadian system in the suprachiasmatic nucleus. J Neurosci 28(18):4619-23. [PubMed: 18448638] [MGI Ref ID J:134974]
Aton SJ; Huettner JE; Straume M; Herzog ED. 2006. GABA and Gi/o differentially control circadian rhythms and synchrony in clock neurons. Proc Natl Acad Sci U S A 103(50):19188-93. [PubMed: 17138670] [MGI Ref ID J:118298]
Beaule C; Swanstrom A; Leone MJ; Herzog ED. 2009. Circadian modulation of gene expression, but not glutamate uptake, in mouse and rat cortical astrocytes. PLoS One 4(10):e7476. [PubMed: 19829696] [MGI Ref ID J:154095]
Castanon-Cervantes O; Wu M; Ehlen JC; Paul K; Gamble KL; Johnson RL; Besing RC; Menaker M; Gewirtz AT; Davidson AJ. 2010. Dysregulation of inflammatory responses by chronic circadian disruption. J Immunol 185(10):5796-805. [PubMed: 20944004] [MGI Ref ID J:165628]
Chen R; Schirmer A; Lee Y; Lee H; Kumar V; Yoo SH; Takahashi JS; Lee C. 2009. Rhythmic PER abundance defines a critical nodal point for negative feedback within the circadian clock mechanism. Mol Cell 36(3):417-30. [PubMed: 19917250] [MGI Ref ID J:154857]
Cheng B; Anea CB; Yao L; Chen F; Patel V; Merloiu A; Pati P; Caldwell RW; Fulton DJ; Rudic RD. 2011. Tissue-intrinsic dysfunction of circadian clock confers transplant arteriosclerosis. Proc Natl Acad Sci U S A 108(41):17147-52. [PubMed: 21969583] [MGI Ref ID J:177448]
Cheng HY; Alvarez-Saavedra M; Dziema H; Choi YS; Li A; Obrietan K. 2009. Segregation of expression of mPeriod gene homologs in neurons and glia: possible divergent roles of mPeriod1 and mPeriod2 in the brain. Hum Mol Genet 18(16):3110-24. [PubMed: 19477955] [MGI Ref ID J:150729]
Cho H; Zhao X; Hatori M; Yu RT; Barish GD; Lam MT; Chong LW; DiTacchio L; Atkins AR; Glass CK; Liddle C; Auwerx J; Downes M; Panda S; Evans RM. 2012. Regulation of circadian behaviour and metabolism by REV-ERB-alpha and REV-ERB-beta. Nature 485(7396):123-7. [PubMed: 22460952] [MGI Ref ID J:183855]
Davidson AJ; Castanon-Cervantes O; Leise TL; Molyneux PC; Harrington ME. 2009. Visualizing jet lag in the mouse suprachiasmatic nucleus and peripheral circadian timing system. Eur J Neurosci 29(1):171-80. [PubMed: 19032592] [MGI Ref ID J:145870]
DeBruyne JP; Weaver DR; Reppert SM. 2007. CLOCK and NPAS2 have overlapping roles in the suprachiasmatic circadian clock. Nat Neurosci 10(5):543-5. [PubMed: 17417633] [MGI Ref ID J:121658]
DeBruyne JP; Weaver DR; Reppert SM. 2007. Peripheral circadian oscillators require CLOCK. Curr Biol 17(14):R538-9. [PubMed: 17637349] [MGI Ref ID J:123965]
Dibner C; Sage D; Unser M; Bauer C; d'Eysmond T; Naef F; Schibler U. 2009. Circadian gene expression is resilient to large fluctuations in overall transcription rates. EMBO J 28(2):123-34. [PubMed: 19078963] [MGI Ref ID J:144225]
Dolatshad H; Cary AJ; Davis FC. 2010. Differential expression of the circadian clock in maternal and embryonic tissues of mice. PLoS One 5(3):e9855. [PubMed: 20352049] [MGI Ref ID J:158725]
Eckle T; Hartmann K; Bonney S; Reithel S; Mittelbronn M; Walker LA; Lowes BD; Han J; Borchers CH; Buttrick PM; Kominsky DJ; Colgan SP; Eltzschig HK. 2012. Adora2b-elicited Per2 stabilization promotes a HIF-dependent metabolic switch crucial for myocardial adaptation to ischemia. Nat Med 18(5):774-82. [PubMed: 22504483] [MGI Ref ID J:183926]
Etchegaray JP; Machida KK; Noton E; Constance CM; Dallmann R; Di Napoli MN; DeBruyne JP; Lambert CM; Yu EA; Reppert SM; Weaver DR. 2009. Casein kinase 1 delta regulates the pace of the mammalian circadian clock. Mol Cell Biol 29(14):3853-66. [PubMed: 19414593] [MGI Ref ID J:150148]
Etchegaray JP; Yu EA; Indic P; Dallmann R; Weaver DR. 2010. Casein kinase 1 delta (CK1delta) regulates period length of the mouse suprachiasmatic circadian clock in vitro. PLoS One 5(4):e10303. [PubMed: 20421981] [MGI Ref ID J:160138]
Foley NC; Tong TY; Foley D; Lesauter J; Welsh DK; Silver R. 2011. Characterization of orderly spatiotemporal patterns of clock gene activation in mammalian suprachiasmatic nucleus. Eur J Neurosci 33(10):1851-65. [PubMed: 21488990] [MGI Ref ID J:177307]
Godinho SI; Maywood ES; Shaw L; Tucci V; Barnard AR; Busino L; Pagano M; Kendall R; Quwailid MM; Romero MR; O'neill J; Chesham JE; Brooker D; Lalanne Z; Hastings MH; Nolan PM. 2007. The after-hours mutant reveals a role for Fbxl3 in determining mammalian circadian period. Science 316(5826):897-900. [PubMed: 17463252] [MGI Ref ID J:121578]
Hirao A; Tahara Y; Kimura I; Shibata S. 2009. A balanced diet is necessary for proper entrainment signals of the mouse liver clock. PLoS One 4(9):e6909. [PubMed: 19738906] [MGI Ref ID J:153628]
Hirota T; Lee JW; Lewis WG; Zhang EE; Breton G; Liu X; Garcia M; Peters EC; Etchegaray JP; Traver D; Schultz PG; Kay SA. 2010. High-throughput chemical screen identifies a novel potent modulator of cellular circadian rhythms and reveals CKIalpha as a clock regulatory kinase. PLoS Biol 8(12):e1000559. [PubMed: 21179498] [MGI Ref ID J:170592]
Hughes AT; Guilding C; Piggins HD. 2011. Neuropeptide Signaling Differentially Affects Phase Maintenance and Rhythm Generation in SCN and Extra-SCN Circadian Oscillators. PLoS One 6(4):e18926. [PubMed: 21559484] [MGI Ref ID J:172445]
Jackson CR; Ruan GX; Aseem F; Abey J; Gamble K; Stanwood G; Palmiter RD; Iuvone PM; McMahon DG. 2012. Retinal Dopamine Mediates Multiple Dimensions of Light-Adapted Vision. J Neurosci 32(27):9359-9368. [PubMed: 22764243] [MGI Ref ID J:185955]
Ko CH; Yamada YR; Welsh DK; Buhr ED; Liu AC; Zhang EE; Ralph MR; Kay SA; Forger DB; Takahashi JS. 2010. Emergence of noise-induced oscillations in the central circadian pacemaker. PLoS Biol 8(10):e1000513. [PubMed: 20967239] [MGI Ref ID J:167074]
Kornmann B; Schaad O; Bujard H; Takahashi JS; Schibler U. 2007. System-driven and oscillator-dependent circadian transcription in mice with a conditionally active liver clock. PLoS Biol 5(2):e34. [PubMed: 17298173] [MGI Ref ID J:141590]
Lee H; Chen R; Lee Y; Yoo S; Lee C. 2009. Essential roles of CKIdelta and CKIepsilon in the mammalian circadian clock. Proc Natl Acad Sci U S A 106(50):21359-64. [PubMed: 19948962] [MGI Ref ID J:155524]
Marcheva B; Ramsey KM; Buhr ED; Kobayashi Y; Su H; Ko CH; Ivanova G; Omura C; Mo S; Vitaterna MH; Lopez JP; Philipson LH; Bradfield CA; Crosby SD; JeBailey L; Wang X; Takahashi JS; Bass J. 2010. Disruption of the clock components CLOCK and BMAL1 leads to hypoinsulinaemia and diabetes. Nature 466(7306):627-31. [PubMed: 20562852] [MGI Ref ID J:162641]
Maywood ES; Fraenkel E; McAllister CJ; Wood N; Reddy AB; Hastings MH; Morton AJ. 2010. Disruption of peripheral circadian timekeeping in a mouse model of Huntington's disease and its restoration by temporally scheduled feeding. J Neurosci 30(30):10199-204. [PubMed: 20668203] [MGI Ref ID J:162853]
Mohawk JA; Baer ML; Menaker M. 2009. The methamphetamine-sensitive circadian oscillator does not employ canonical clock genes. Proc Natl Acad Sci U S A 106(9):3519-24. [PubMed: 19204282] [MGI Ref ID J:146448]
Nakamura TJ; Sellix MT; Menaker M; Block GD. 2008. Estrogen directly modulates circadian rhythms of PER2 expression in the uterus. Am J Physiol Endocrinol Metab 295(5):E1025-31. [PubMed: 18728223] [MGI Ref ID J:142256]
Nishide SY; Ono D; Yamada Y; Honma S; Honma K. 2012. De novo synthesis of PERIOD initiates circadian oscillation in cultured mouse suprachiasmatic nucleus after prolonged inhibition of protein synthesis by cycloheximide. Eur J Neurosci 35(2):291-9. [PubMed: 22236088] [MGI Ref ID J:184332]
Oike H; Nagai K; Fukushima T; Ishida N; Kobori M. 2011. Feeding cues and injected nutrients induce acute expression of multiple clock genes in the mouse liver. PLoS One 6(8):e23709. [PubMed: 21901130] [MGI Ref ID J:176138]
Reale ME; Webb IC; Wang X; Baltazar RM; Coolen LM; Lehman MN. 2013. The transcription factor Runx2 is under circadian control in the suprachiasmatic nucleus and functions in the control of rhythmic behavior. PLoS One 8(1):e54317. [PubMed: 23372705] [MGI Ref ID J:195796]
Ruan GX; Allen GC; Yamazaki S; McMahon DG. 2008. An autonomous circadian clock in the inner mouse retina regulated by dopamine and GABA. PLoS Biol 6(10):e249. [PubMed: 18959477] [MGI Ref ID J:141081]
Shimomura K; Lowrey PL; Vitaterna MH; Buhr ED; Kumar V; Hanna P; Omura C; Izumo M; Low SS; Barrett RK; LaRue SI; Green CB; Takahashi JS. 2010. Genetic suppression of the circadian Clock mutation by the melatonin biosynthesis pathway. Proc Natl Acad Sci U S A 107(18):8399-403. [PubMed: 20404168] [MGI Ref ID J:160321]
Webb AB; Angelo N; Huettner JE; Herzog ED. 2009. Intrinsic, nondeterministic circadian rhythm generation in identified mammalian neurons. Proc Natl Acad Sci U S A 106(38):16493-8. [PubMed: 19805326] [MGI Ref ID J:153227]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice are bred as homozygotes. Mating System Homozygote x Homozygote (Female x Male) 12-FEB-08 Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $177.00 Female or Male Homozygous for Per2tm1Jt
Price per Pair (US dollars $) Pair Genotype $354.00 Homozygous for Per2tm1Jt x Homozygous for Per2tm1Jt Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $230.10 Female or Male Homozygous for Per2tm1Jt
Price per Pair (US dollars $) Pair Genotype $460.20 Homozygous for Per2tm1Jt x Homozygous for Per2tm1Jt Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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|
|
Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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