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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Homozygote (Female x Male) 12-FEB-08 Species laboratory mouse Generation N11+F5 (08-JAN-09) Donating Investigator Joseph Takahashi, Univ Texas Southwestern Medical Ctr Description
Mice homozygous for this "mPer2Luc" mutation are viable and fertile with no developmental or morphological differences compared to wildtype littermates. Expression of PERIOD2::LUCIFERASE (or mPER2::LUC) fusion protein during peak periods is similar to endogenous pPER2 patterns. Homozygous mice have normal entrainment and circadian behaviors. These mPer2Luc knock-in mice may be useful as a real-time reporter of circadian dynamics in different tissues.Development
A targeting vector was designed to insert the firefly luciferase (luc) gene (followed by a loxP-flanked neomycin resistance gene) in-frame into exon 23 of the wild-type sequence. The construct was electroporated into 129S6/SvEvTac-derived W4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. Chimeric males were bred to C57BL/6J females. Heterozygotes were backcrossed to C57BL/6J inbred mice for 7-11 generations prior to arrival at The Jackson Laboratory.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (225 strains)
010832 129S-Per2tm1Drw/J 003819 B6.129S7-Per2tm1Brd/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Per2tm1Jt/Per2tm1Jt
involves: 129S6/SvEvTac * C57BL/6J
- normal phenotype
- no abnormal phenotype detected (MGI Ref ID J:89256)
- mice developed normally and showed no overt morphological abnormalities
This mouse can be used to support research in many areas including:
Neurobiology Research
Circadian Rhythms
Research Tools
Fluorescent Proteins
Neurobiology Research
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Per2tm1Jt | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Joseph S Takahashi | ||
| Allele Type | Targeted (Reporter) | ||
| Common Name(s) | mPER2::LUC; mPer2Luc; mPer2Luciferase; | ||
| Mutation Made By | Joseph Takahashi, Univ Texas Southwestern Medical Ctr | ||
| Strain of Origin | 129S6/SvEvTac | ||
| ES Cell Line Name | W4 | ||
| ES Cell Line Strain | 129S6/SvEvTac | ||
| Site of Expression | Circadian patterns of Per2-Luc fusion protein expression is seen in the following peripheral tissues: SCN, cornea, liver, pituitary, kidney, RCA, lung and tail). | ||
| Gene Symbol and Name | Per2, period homolog 2 (Drosophila) | ||
| Chromosome | 1 | ||
| Gene Common Name(s) | FASPS; KIAA0347; mKIAA0347; mPer2; rPER2; | ||
| Molecular Note | A luciferase gene and floxed neo cassette were inserted between exon 23 and the 3' UTR. Mutant transcript was detected in Northern blot of mutant brains and the Per2-Luc fusion protein was detected in Western blots of mutant kidneys. [MGI Ref ID J:89256] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Per2tm1Jt, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Yoo SH; Yamazaki S; Lowrey PL; Shimomura K; Ko CH; Buhr ED; Siepka SM; Hong HK; Oh WJ; Yoo OJ; Menaker M; Takahashi JS. 2004. PERIOD2::LUCIFERASE real-time reporting of circadian dynamics reveals persistent circadian oscillations in mouse peripheral tissues. Proc Natl Acad Sci U S A 101(15):5339-46. [PubMed: 14963227] [MGI Ref ID J:89256]
Additional References
Per2tm1Jt relatedAkashi M; Hayasaka N; Yamazaki S; Node K. 2008. Mitogen-activated protein kinase is a functional component of the autonomous circadian system in the suprachiasmatic nucleus. J Neurosci 28(18):4619-23. [PubMed: 18448638] [MGI Ref ID J:134974]
Aton SJ; Huettner JE; Straume M; Herzog ED. 2006. GABA and Gi/o differentially control circadian rhythms and synchrony in clock neurons. Proc Natl Acad Sci U S A 103(50):19188-93. [PubMed: 17138670] [MGI Ref ID J:118298]
Beaule C; Swanstrom A; Leone MJ; Herzog ED. 2009. Circadian modulation of gene expression, but not glutamate uptake, in mouse and rat cortical astrocytes. PLoS One 4(10):e7476. [PubMed: 19829696] [MGI Ref ID J:154095]
Cheng HY; Alvarez-Saavedra M; Dziema H; Choi YS; Li A; Obrietan K. 2009. Segregation of expression of mPeriod gene homologs in neurons and glia: possible divergent roles of mPeriod1 and mPeriod2 in the brain. Hum Mol Genet 18(16):3110-24. [PubMed: 19477955] [MGI Ref ID J:150729]
Davidson AJ; Castanon-Cervantes O; Leise TL; Molyneux PC; Harrington ME. 2009. Visualizing jet lag in the mouse suprachiasmatic nucleus and peripheral circadian timing system. Eur J Neurosci 29(1):171-80. [PubMed: 19032592] [MGI Ref ID J:145870]
DeBruyne JP; Weaver DR; Reppert SM. 2007. CLOCK and NPAS2 have overlapping roles in the suprachiasmatic circadian clock. Nat Neurosci 10(5):543-5. [PubMed: 17417633] [MGI Ref ID J:121658]
DeBruyne JP; Weaver DR; Reppert SM. 2007. Peripheral circadian oscillators require CLOCK. Curr Biol 17(14):R538-9. [PubMed: 17637349] [MGI Ref ID J:123965]
Dibner C; Sage D; Unser M; Bauer C; d'Eysmond T; Naef F; Schibler U. 2009. Circadian gene expression is resilient to large fluctuations in overall transcription rates. EMBO J 28(2):123-34. [PubMed: 19078963] [MGI Ref ID J:144225]
Etchegaray JP; Machida KK; Noton E; Constance CM; Dallmann R; Di Napoli MN; DeBruyne JP; Lambert CM; Yu EA; Reppert SM; Weaver DR. 2009. Casein kinase 1 delta regulates the pace of the mammalian circadian clock. Mol Cell Biol 29(14):3853-66. [PubMed: 19414593] [MGI Ref ID J:150148]
Godinho SI; Maywood ES; Shaw L; Tucci V; Barnard AR; Busino L; Pagano M; Kendall R; Quwailid MM; Romero MR; O'neill J; Chesham JE; Brooker D; Lalanne Z; Hastings MH; Nolan PM. 2007. The after-hours mutant reveals a role for Fbxl3 in determining mammalian circadian period. Science 316(5826):897-900. [PubMed: 17463252] [MGI Ref ID J:121578]
Hirao A; Tahara Y; Kimura I; Shibata S. 2009. A balanced diet is necessary for proper entrainment signals of the mouse liver clock. PLoS One 4(9):e6909. [PubMed: 19738906] [MGI Ref ID J:153628]
Kornmann B; Schaad O; Bujard H; Takahashi JS; Schibler U. 2007. System-driven and oscillator-dependent circadian transcription in mice with a conditionally active liver clock. PLoS Biol 5(2):e34. [PubMed: 17298173] [MGI Ref ID J:141590]
Mohawk JA; Baer ML; Menaker M. 2009. The methamphetamine-sensitive circadian oscillator does not employ canonical clock genes. Proc Natl Acad Sci U S A 106(9):3519-24. [PubMed: 19204282] [MGI Ref ID J:146448]
Nakamura TJ; Sellix MT; Menaker M; Block GD. 2008. Estrogen directly modulates circadian rhythms of PER2 expression in the uterus. Am J Physiol Endocrinol Metab 295(5):E1025-31. [PubMed: 18728223] [MGI Ref ID J:142256]
Ruan GX; Allen GC; Yamazaki S; McMahon DG. 2008. An autonomous circadian clock in the inner mouse retina regulated by dopamine and GABA. PLoS Biol 6(10):e249. [PubMed: 18959477] [MGI Ref ID J:141081]
Webb AB; Angelo N; Huettner JE; Herzog ED. 2009. Intrinsic, nondeterministic circadian rhythm generation in identified mammalian neurons. Proc Natl Acad Sci U S A 106(38):16493-8. [PubMed: 19805326] [MGI Ref ID J:153227]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice are bred as homozygotes. Mating System Homozygote x Homozygote (Female x Male) 12-FEB-08 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $171.30 Female or Male Homozygous for Per2tm1Jt
Pairs /Price (US dollars $) Pair Genotype $342.60 Homozygous for Per2tm1Jt x Homozygous for Per2tm1Jt
Additional Supply Details
| Pricing for International shipping destinations |
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Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $222.70 Female or Male Homozygous for Per2tm1Jt
Pairs /Price (US dollars $) Pair Genotype $445.40 Homozygous for Per2tm1Jt x Homozygous for Per2tm1Jt
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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