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- Description
- Phenotype
- Genes & alleles
- Genotyping
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Description
Strain Information
Type Congenic; Mutant Strain; Targeted Mutation; Transgenic; Additional information on Genetically Engineered Mutant Mice. Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Generation N10N1 (03-DEC-07) Donating Investigator Manami Hara, University of Chicago Description
Mice hemizygous for this "MIP-GFP" transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Enhanced Green Fluorescent Protein (EGFP) under the control of the mouse insulin 1 promoter. Fluorescence is detected in tissues where insulin I is normally expressed; fluorescent protein expression in pancreatic beta-cells is evident from embryonic day (E)13.5 through adulthood. The fluorescence expression pattern is similar to the patterns seen in other stocks (see Stock No. 006784 and Stock No. 006866). MIP-GFP transgenic mice exhibit normal glucose tolerance and pancreatic insulin levels. The human growth hormone (hGH) sequence in the transgenic insert enhances expression of the EGFP, but hGH is not expressed. This mutant mouse strain may be useful in studies of diabetes and pancreatic beta islet cell biology.In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Development
A transgenic construct containing the coding region of the EGFP gene and a 2.1kb sequence of the human growth hormone to confer high expression of the EGFP under the control of the mouse insulin 1 gene promoter was injected into fertilized CD1 mouse eggs. Founder line 1 was subsequently established. The transgenic mice were then backcrossed to C57BL/6 for 10 generations.
Control Information
| Control | ||
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| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (169 strains)
008173 NOD.Cg-Tg(Ins1-EGFP)1Hara/QtngJ
Additional Web Information
Congenic Nomenclature
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
Tg(Ins1-EGFP)1Hara/0
involves: CD-1
- normal phenotype
- no abnormal phenotype detected (MGI Ref ID J:120245)
- no phenotypic abnormalities reported
This mouse can be used to support research in many areas including:
GFP relatedResearch Tools
Diabetes and Obesity Research
Fluorescent Proteins
Genetics Research (Tissue/Cell Markers: pancreatic beta cells)
Ins1 relatedResearch Tools
Fluorescent Proteins
Diabetes and Obesity Research
Type 1 Diabetes (IDDM) Analysis Strains (NOD/ShiLtJ Non-MHC Congenics)
Immunology and Inflammation Research
Autoimmunity (Type 1 Diabetes)
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Ins1-EGFP)1Hara | ||
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| Allele Name | transgene insertion 1, Manami Hara | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | MIP-GFP; MIP-GFP (line 1); MIP-GFP (line1); MIP:GFP; | ||
| Mutation Made By | Manami Hara, University of Chicago | ||
| Strain of Origin | CD-1 | ||
| Site of Expression | EGFP expression is detected in tissues where insulin I is normally expressed; fluorescent protein expression in pancreatic beta-cells is evident from embryonic day (E)13.5 through adulthood. | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | Ins1, insulin I, mouse, laboratory | ||
| Molecular Note | A transgenic construct containing the coding region of the EGFP gene and a 2.1kb sequence of the human growth hormone to confer high expression of the EGFP, under the control of the mouse insulin 1 gene promoter (a 8.5 kb region including -8.5 to +12 bp relative to the transcriptional start site) was injected into fertilized CD1 mouse eggs. Founder line 1 was subsequently established. Fluorescence is detected in tissues where insulin I is normally expressed; fluorescent protein expression in pancreatic beta-cells is evident from embryonic day (E)13.5 through adult. [MGI Ref ID J:99450] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Fluorescent Proteins (Generic GFP), STD PCR, vers. 1
Fluorescent Proteins -- Generic GFP, QPCR, vers. 1
Fluorescent Proteins -- Generic GFP, QPCR, vers. 5
TG(DTR/EGFP), STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Hara M; Wang X; Kawamura T; Bindokas VP; Dizon RF; Alcoser SY; Magnuson MA; Bell GI. 2003. Transgenic mice with green fluorescent protein-labeled pancreatic beta -cells. Am J Physiol Endocrinol Metab 284(1):E177-83. [PubMed: 12388130] [MGI Ref ID J:99450]
Additional References
Hara M; Dizon RF; Glick BS; Lee CS; Kaestner KH; Piston DW; Bindokas VP. 2006. Imaging pancreatic beta-cells in the intact pancreas. Am J Physiol Endocrinol Metab 290(5):E1041-7. [PubMed: 16368785] [MGI Ref ID J:120245]
Tg(Ins1-EGFP)1Hara relatedBurlison JS; Long Q; Fujitani Y; Wright CV; Magnuson MA. 2008. Pdx-1 and Ptf1a concurrently determine fate specification of pancreatic multipotent progenitor cells. Dev Biol 316(1):74-86. [PubMed: 18294628] [MGI Ref ID J:135698]
Chase LG; Ulloa-Montoya F; Kidder BL; Verfaillie CM. 2007. Islet-derived fibroblast-like cells are not derived via epithelial-mesenchymal transition from Pdx-1 or insulin-positive cells. Diabetes 56(1):3-7. [PubMed: 17110468] [MGI Ref ID J:121938]
Diao J; Allister EM; Koshkin V; Lee SC; Bhattacharjee A; Tang C; Giacca A; Chan CB; Wheeler MB. 2008. UCP2 is highly expressed in pancreatic alpha-cells and influences secretion and survival. Proc Natl Acad Sci U S A 105(33):12057-62. [PubMed: 18701716] [MGI Ref ID J:138987]
Hara M; Dizon RF; Glick BS; Lee CS; Kaestner KH; Piston DW; Bindokas VP. 2006. Imaging pancreatic beta-cells in the intact pancreas. Am J Physiol Endocrinol Metab 290(5):E1041-7. [PubMed: 16368785] [MGI Ref ID J:120245]
Hauser AE; Junt T; Mempel TR; Sneddon MW; Kleinstein SH; Henrickson SE; von Andrian UH; Shlomchik MJ; Haberman AM. 2007. Definition of germinal-center B cell migration in vivo reveals predominant intrazonal circulation patterns. Immunity 26(5):655-67. [PubMed: 17509908] [MGI Ref ID J:123545]
Mwangi S; Anitha M; Mallikarjun C; Ding X; Hara M; Parsadanian A; Larsen CP; Thule P; Sitaraman SV; Anania F; Srinivasan S. 2008. Glial cell line-derived neurotrophic factor increases beta-cell mass and improves glucose tolerance. Gastroenterology 134(3):727-37. [PubMed: 18241861] [MGI Ref ID J:135600]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Mating System Homozygote x Homozygote (Female x Male) Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $203.80 Female or Male Homozygous for Tg(Ins1-EGFP)1Hara *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $407.60 Homozygous for Tg(Ins1-EGFP)1Hara x Homozygous for Tg(Ins1-EGFP)1Hara
Additional Supply Details
| Supply Notes |
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| Pricing for International shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $265.00 Female or Male Homozygous for Tg(Ins1-EGFP)1Hara *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $529.90 Homozygous for Tg(Ins1-EGFP)1Hara x Homozygous for Tg(Ins1-EGFP)1Hara
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
See Terms of Use
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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