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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Congenic; Mutant Strain; Targeted Mutation; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Homozygote (Female x Male) 18-JAN-08 Species laboratory mouse Generation N10F11 (30-JUN-11)
Generation DefinitionsDonating Investigator Manami Hara, University of Chicago Description
Mice hemizygous for this "MIP-GFP" transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Enhanced Green Fluorescent Protein (EGFP) under the control of the mouse insulin 1 promoter. Fluorescence is detected in tissues where insulin I is normally expressed; fluorescent protein expression in pancreatic beta-cells is evident from embryonic day (E)13.5 through adulthood. The fluorescence expression pattern is similar to the patterns seen in other stocks (see Stock No. 006784 and Stock No. 006866). MIP-GFP transgenic mice exhibit normal glucose tolerance and pancreatic insulin levels. The human growth hormone (hGH) sequence in the transgenic insert enhances expression of the EGFP, but hGH is not expressed. This mutant mouse strain may be useful in studies of diabetes and pancreatic beta islet cell biology.In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Development
A transgenic construct containing the coding region of the EGFP gene and a 2.1kb sequence of the human growth hormone to confer high expression of the EGFP under the control of the mouse insulin 1 gene promoter was injected into fertilized CD1 mouse eggs. Founder line 1 was subsequently established. The transgenic mice were then backcrossed to C57BL/6 for 10 generations (see SNp note below).A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 2 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (345 strains)
008173 NOD.Cg-Tg(Ins1-EGFP)1Hara/QtngJ
008173 NOD.Cg-Tg(Ins1-EGFP)1Hara/QtngJ
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
Tg(Ins1-EGFP)1Hara/0
involves: CD-1
- normal phenotype
- no abnormal phenotype detected
- no phenotypic abnormalities reported (MGI Ref ID J:120245)
This mouse can be used to support research in many areas including:
GFP relatedResearch Tools
Diabetes and Obesity Research
Fluorescent Proteins
Genetics Research
Tissue/Cell Markers
Tissue/Cell Markers: pancreatic beta cells
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Ins1-EGFP)1Hara | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Manami Hara | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | MIP-EGFP; MIP-GFP; MIP-GFP (line 1); MIP-GFP (line1); MIP:GFP; | ||
| Mutation Made By | Manami Hara, University of Chicago | ||
| Strain of Origin | CD-1 | ||
| Site of Expression | EGFP expression is detected in tissues where insulin I is normally expressed; fluorescent protein expression in pancreatic beta-cells is evident from embryonic day (E)13.5 through adulthood. | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | Ins1, insulin I, mouse, laboratory | ||
| Molecular Note | A transgenic construct containing the coding region of the EGFP gene and a 2.1kb sequence of the human growth hormone to confer high expression of the EGFP, under the control of the mouse insulin 1 gene promoter (a 8.5 kb region including -8.5 to +12 bp relative to the transcriptional start site) was injected into fertilized CD1 mouse eggs. Founder line 1 was subsequently established. Fluorescence is detected in tissues where insulin I is normally expressed; fluorescent protein expression in pancreatic beta-cells is evident from embryonic day (E)13.5 through adult. [MGI Ref ID J:99450] | ||
| Gene Symbol and Name | Tg(Ins1-EGFP)1Hara, transgene insertion 1, Manami Hara | ||
| Chromosome | UN | ||
| Gene Common Name(s) | MIP-GFP; MIP-GFP (line 1); MIP-GFP (line1); MIP:GFP; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Fluorescent Proteins (Generic GFP), Standard PCR
Fluorescent Proteins -- Generic GFP, QPCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Hara M; Wang X; Kawamura T; Bindokas VP; Dizon RF; Alcoser SY; Magnuson MA; Bell GI. 2003. Transgenic mice with green fluorescent protein-labeled pancreatic beta -cells. Am J Physiol Endocrinol Metab 284(1):E177-83. [PubMed: 12388130] [MGI Ref ID J:99450]
Additional References
Hara M; Dizon RF; Glick BS; Lee CS; Kaestner KH; Piston DW; Bindokas VP. 2006. Imaging pancreatic beta-cells in the intact pancreas. Am J Physiol Endocrinol Metab 290(5):E1041-7. [PubMed: 16368785] [MGI Ref ID J:120245]
Tg(Ins1-EGFP)1Hara relatedBurlison JS; Long Q; Fujitani Y; Wright CV; Magnuson MA. 2008. Pdx-1 and Ptf1a concurrently determine fate specification of pancreatic multipotent progenitor cells. Dev Biol 316(1):74-86. [PubMed: 18294628] [MGI Ref ID J:135698]
Chase LG; Ulloa-Montoya F; Kidder BL; Verfaillie CM. 2007. Islet-derived fibroblast-like cells are not derived via epithelial-mesenchymal transition from Pdx-1 or insulin-positive cells. Diabetes 56(1):3-7. [PubMed: 17110468] [MGI Ref ID J:121938]
Chen H; Gu X; Liu Y; Wang J; Wirt SE; Bottino R; Schorle H; Sage J; Kim SK. 2011. PDGF signalling controls age-dependent proliferation in pancreatic beta-cells. Nature 478(7369):349-55. [PubMed: 21993628] [MGI Ref ID J:177411]
Diao J; Allister EM; Koshkin V; Lee SC; Bhattacharjee A; Tang C; Giacca A; Chan CB; Wheeler MB. 2008. UCP2 is highly expressed in pancreatic alpha-cells and influences secretion and survival. Proc Natl Acad Sci U S A 105(33):12057-62. [PubMed: 18701716] [MGI Ref ID J:138987]
Hara M; Dizon RF; Glick BS; Lee CS; Kaestner KH; Piston DW; Bindokas VP. 2006. Imaging pancreatic beta-cells in the intact pancreas. Am J Physiol Endocrinol Metab 290(5):E1041-7. [PubMed: 16368785] [MGI Ref ID J:120245]
Hauser AE; Junt T; Mempel TR; Sneddon MW; Kleinstein SH; Henrickson SE; von Andrian UH; Shlomchik MJ; Haberman AM. 2007. Definition of germinal-center B cell migration in vivo reveals predominant intrazonal circulation patterns. Immunity 26(5):655-67. [PubMed: 17509908] [MGI Ref ID J:123545]
Inada A; Nienaber C; Katsuta H; Fujitani Y; Levine J; Morita R; Sharma A; Bonner-Weir S. 2008. Carbonic anhydrase II-positive pancreatic cells are progenitors for both endocrine and exocrine pancreas after birth. Proc Natl Acad Sci U S A 105(50):19915-9. [PubMed: 19052237] [MGI Ref ID J:142917]
Jacobson DA; Mendez F; Thompson M; Torres J; Cochet O; Philipson LH. 2010. Calcium-activated and voltage-gated potassium channels of the pancreatic islet impart distinct and complementary roles during secretagogue induced electrical responses. J Physiol 588(Pt 18):3525-37. [PubMed: 20643768] [MGI Ref ID J:177330]
Kilimnik G; Kim A; Jo J; Miller K; Hara M. 2009. Quantification of pancreatic islet distribution in situ in mice. Am J Physiol Endocrinol Metab 297(6):E1331-8. [PubMed: 19808908] [MGI Ref ID J:159564]
Melli K; Friedman RS; Martin AE; Finger EB; Miao G; Szot GL; Krummel MF; Tang Q. 2009. Amplification of autoimmune response through induction of dendritic cell maturation in inflamed tissues. J Immunol 182(5):2590-600. [PubMed: 19234153] [MGI Ref ID J:145529]
Miyatsuka T; Kosaka Y; Kim H; German MS. 2011. Neurogenin3 inhibits proliferation in endocrine progenitors by inducing Cdkn1a. Proc Natl Acad Sci U S A 108(1):185-90. [PubMed: 21173230] [MGI Ref ID J:169013]
Mwangi S; Anitha M; Mallikarjun C; Ding X; Hara M; Parsadanian A; Larsen CP; Thule P; Sitaraman SV; Anania F; Srinivasan S. 2008. Glial cell line-derived neurotrophic factor increases beta-cell mass and improves glucose tolerance. Gastroenterology 134(3):727-37. [PubMed: 18241861] [MGI Ref ID J:135600]
Nyman LR; Ford E; Powers AC; Piston DW. 2010. Glucose-dependent blood flow dynamics in murine pancreatic islets in vivo. Am J Physiol Endocrinol Metab 298(4):E807-14. [PubMed: 20071562] [MGI Ref ID J:162894]
Smukler SR; Arntfield ME; Razavi R; Bikopoulos G; Karpowicz P; Seaberg R; Dai F; Lee S; Ahrens R; Fraser PE; Wheeler MB; van der Kooy D. 2011. The adult mouse and human pancreas contain rare multipotent stem cells that express insulin. Cell Stem Cell 8(3):281-93. [PubMed: 21362568] [MGI Ref ID J:171126]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Mating System Homozygote x Homozygote (Female x Male) 18-JAN-08 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $225.00 Female or Male Homozygous for Tg(Ins1-EGFP)1Hara
Pairs /Price (US dollars $) Pair Genotype $450.00 Homozygous for Tg(Ins1-EGFP)1Hara x Homozygous for Tg(Ins1-EGFP)1Hara Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $292.50 Female or Male Homozygous for Tg(Ins1-EGFP)1Hara
Pairs /Price (US dollars $) Pair Genotype $585.00 Homozygous for Tg(Ins1-EGFP)1Hara x Homozygous for Tg(Ins1-EGFP)1Hara Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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