Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N6+F1pN1
Donating Investigator Richard Quarles, NINDS, NIH
Mice homozygous for this targeted mutation are viable and fertile. The endogenous protein had previously been thought to be necessary for myelin formation. However in the homozygous mutant the degree of myelination and its compaction are normal. Finer motor coordination abilities are significantly affected in the homozygous mutant and they exhibit a subtle intention tremor. The organization of the periaxonal region is partially impaired with the periaxonal cytoplasmic collar frequently missing in optic nerve, cervical spinal cord, and ventral roots. Later in life, beginning at 6 months, oligodendrocytes degenerate. This strain may serve as a model for some aspects of multiple sclerosis. MAG also tranduces a signal to axons. Therefore, axons in the MAG-deficient mice are smaller in calliber due to the aberrant phosphorylation of neurofilaments. MAG has also been shown to be an inhibitor of nerve regeneration. MAG-deficient mice congenic on a C57BL/6 background may exhibit substantially more degeneration of CNS axons compared to deficiency on the original mixed background.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
The targeted mutation was created by Dr. John Roder (University of Toronto) by designing a targeting vector to insert a neomycin resistance cassette into exon 5 of the targeted gene. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. The original strain (see Stock No. 002403) on a mixed C57BL/6, 129 inbred and the CD1 outbred genetic background was sent to Dr. Richard Quarles (NINDS/NIH). There, mutant mice were backcrossed for at least 13 generations prior to arrival at The Jackson Laboratory.
|Considerations for Choosing Controls|
Strains carrying Magtm1Rod allele
002403 STOCK Magtm1Rod/JView Strains carrying Magtm1Rod (1 strain)Strains carrying other alleles of Mag
025187 B6N(Cg)-Magtm1.1(KOMP)Vlcg/JView Strains carrying other alleles of Mag (1 strain)
View Mammalian Phenotype TermsMammalian Phenotype Terms provided by MGIassigned by genotype
The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.
Magtm1Rod/Magtm1Rodinvolves: 129S1/Sv * 129X1/SvJ
- nervous system phenotype
- abnormal axon morphology
- optic nerve, cervical spinal cord, and ventral roots exhibit frequent loss of periaxonal cytoplasmic collar, periaxonal collar swelling, cytoplasm within myelin, and redundant myelin, however degree of myelination and its compaction are normal (MGI Ref ID J:18407)
- the cytoplasmic leaflet of the periaxonal membrane is often fused with the inner compact myelin lamellae to form a dense line (MGI Ref ID J:18407)
- when present, the cytoplasm of the periaxonal collar is usually disorganized (MGI Ref ID J:18407)
- behavior/neurological phenotype
- abnormal grooming behavior
- less frequent exploratory sniffing and grooming on a bar-cross apparatus (MGI Ref ID J:18407)
- show decreased locomotor activity on a bar-cross apparatus (MGI Ref ID J:18407)
- impaired coordination
- slower to transverse a narrow challenge bar (MGI Ref ID J:18407)
- many homozygotes exhibit a mild, transient, trunk tremor on a bar-cross apparatus (MGI Ref ID J:18407)View Research Applications
|Allele Name||targeted mutation 1, John Roder|
|Allele Type||Targeted (Null/Knockout)|
|Mutation Made By||Dr. John Roder, University of Toronto|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl<+>|
|ES Cell Line Name||R1|
|ES Cell Line Strain||(129X1/SvJ x 129S1/Sv)F1-Kitl<+>|
|Gene Symbol and Name||Mag, myelin-associated glycoprotein|
|Gene Common Name(s)||GMA; Gma; S-MAG; SIGLEC-4A; SIGLEC4A;|
|Molecular Note||A neomycin resistance cassette was inserted into exon 5 of the gene. Mutant mice showed a complete lack of mRNA and protein in the central nervous system for the targeted gene. Nerves from the peripheral nervous system also lacked protein for the targeted gene. [MGI Ref ID J:18407]|
Li C; Tropak MB; Gerlai R; Clapoff S; Abramow-Newerly W; Trapp B; Peterson A; Roder J. 1994. Myelination in the absence of myelin-associated glycoprotein. Nature 369(6483):747-50. [PubMed: 7516497] [MGI Ref ID J:18407]
Pan B; Fromholt SE; Hess EJ; Crawford TO; Griffin JW; Sheikh KA; Schnaar RL. 2005. Myelin-associated glycoprotein and complementary axonal ligands, gangliosides, mediate axon stability in the CNS and PNS: neuropathology and behavioral deficits in single- and double-null mice. Exp Neurol 195(1):208-17. [PubMed: 15953602] [MGI Ref ID J:100940]
Cafferty WB; Duffy P; Huebner E; Strittmatter SM. 2010. MAG and OMgp synergize with Nogo-A to restrict axonal growth and neurological recovery after spinal cord trauma. J Neurosci 30(20):6825-37. [PubMed: 20484625] [MGI Ref ID J:160516]
Dickendesher TL; Baldwin KT; Mironova YA; Koriyama Y; Raiker SJ; Askew KL; Wood A; Geoffroy CG; Zheng B; Liepmann CD; Katagiri Y; Benowitz LI; Geller HM; Giger RJ. 2012. NgR1 and NgR3 are receptors for chondroitin sulfate proteoglycans. Nat Neurosci 15(5):703-12. [PubMed: 22406547] [MGI Ref ID J:191258]
Duffy P; Wang X; Siegel CS; Tu N; Henkemeyer M; Cafferty WB; Strittmatter SM. 2012. Myelin-derived ephrinB3 restricts axonal regeneration and recovery after adult CNS injury. Proc Natl Acad Sci U S A 109(13):5063-8. [PubMed: 22411787] [MGI Ref ID J:182224]
Ewaleifoh O; Trinh M; Griffin JW; Nguyen T. 2012. A novel system to accelerate the progression of nerve degeneration in transgenic mouse models of neuropathies. Exp Neurol 237(1):153-9. [PubMed: 22688009] [MGI Ref ID J:187069]
Haney CA; Sahenk Z; Li C; Lemmon VP; Roder J; Trapp BD. 1999. Heterophilic binding of L1 on unmyelinated sensory axons mediates Schwann cell adhesion and is required for axonal survival. J Cell Biol 146(5):1173-84. [PubMed: 10477768] [MGI Ref ID J:57603]
Khazaei MR; Halfter H; Karimzadeh F; Koo JH; Margolis FL; Young P. 2010. Bex1 is involved in the regeneration of axons after injury. J Neurochem 115(4):910-20. [PubMed: 20731761] [MGI Ref ID J:166463]
Kumar S; Yin X; Trapp BD; Paulaitis ME; Hoh JH. 2002. Role of long-range repulsive forces in organizing axonal neurofilament distributions: evidence from mice deficient in myelin-associated glycoprotein. J Neurosci Res 68(6):681-90. [PubMed: 12111829] [MGI Ref ID J:104964]
Lee JK; Geoffroy CG; Chan AF; Tolentino KE; Crawford MJ; Leal MA; Kang B; Zheng B. 2010. Assessing spinal axon regeneration and sprouting in Nogo-, MAG-, and OMgp-deficient mice. Neuron 66(5):663-70. [PubMed: 20547125] [MGI Ref ID J:163185]
Lopez PH; Ahmad AS; Mehta NR; Toner M; Rowland EA; Zhang J; Dore S; Schnaar RL. 2011. Myelin-associated glycoprotein protects neurons from excitotoxicity. J Neurochem 116(5):900-8. [PubMed: 21214567] [MGI Ref ID J:170525]
Marcus J; Dupree JL; Popko B. 2002. Myelin-associated glycoprotein and myelin galactolipids stabilize developing axo-glial interactions. J Cell Biol 156(3):567-77. [PubMed: 11827985] [MGI Ref ID J:77227]
Nguyen T; Mehta NR; Conant K; Kim KJ; Jones M; Calabresi PA; Melli G; Hoke A; Schnaar RL; Ming GL; Song H; Keswani SC; Griffin JW. 2009. Axonal protective effects of the myelin-associated glycoprotein. J Neurosci 29(3):630-7. [PubMed: 19158290] [MGI Ref ID J:144847]
Uschkureit T; Sporkel O; Stracke J; Bussow H; Stoffel W. 2000. Early onset of axonal degeneration in double (plp-/-mag-/-) and hypomyelinosis in triple (plp-/-mbp-/-mag-/-) mutant mice. J Neurosci 20(14):5225-33. [PubMed: 10884306] [MGI Ref ID J:63480]
Vabnick I; Messing A; Chiu SY; Levinson SR; Schachner M; Roder J; Li C; Novakovic S; Shrager P. 1997. Sodium channel distribution in axons of hypomyelinated and MAG null mutant mice. J Neurosci Res 50(2):321-36. [PubMed: 9373041] [MGI Ref ID J:44044]
Yin X; Crawford TO; Griffin JW; Tu P; Lee VM; Li C; Roder J; Trapp BD. 1998. Myelin-associated glycoprotein is a myelin signal that modulates the caliber of myelinated axons. J Neurosci 18(6):1953-62. [PubMed: 9482781] [MGI Ref ID J:160879]
Yin X; Kidd GJ; Nave KA; Trapp BD. 2008. P0 protein is required for and can induce formation of schmidt-lantermann incisures in myelin internodes. J Neurosci 28(28):7068-73. [PubMed: 18614675] [MGI Ref ID J:137961]
Animal Health ReportsProduction of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.
Breeding & Husbandry When maintaining a live colony, homozygous mice are bred.
|Pricing for USA, Canada and Mexico shipping destinations|
Cryopreserved Mice - Ready for Recovery
Price (US dollars $) Cryorecovery* $2140.00
At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
|Pricing for International shipping destinations|
Cryopreserved Mice - Ready for Recovery
Price (US dollars $) Cryorecovery* $2782.00
Cryorecovery - Standard.
Progeny testing is not required.
|Considerations for Choosing Controls|
|Control Pricing Information for Genetically Engineered Mutant Strains.|
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