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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Mutant Stock; Transgenic; Additional information on Genetically Engineered Mutant Mice. Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Generation F?+N1F1 (20-DEC-07) Donating Investigator Manami Hara, University of Chicago Description
Mice hemizygous for this "MIP-RFP" transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express the DsRed.T4 variant of Enhanced Red Fluorescent Protein under the control of mouse insulin I promoter (MIP). Fluorescence is detected in tissues where insulin I is normally expressed; fluorescent protein expression in pancreatic beta-cells is evident from embryonic day (E)13.5 through adulthood. Transgenic mice exhibit normal glucose tolerance and pancreatic insulin levels. The human growth hormone sequence in the transgene is designed to enhance expression of the fluorescent protein. Although the expression of this sequence in this strain has not been characterized, the sequence is not expressed in a similarly constructed transgenic strain (see Stock No. 006864). This mutant mouse strain may be useful in studies of pancreatic development and pancreatic beta islet cell biology.Development
A transgenic construct containing the Red Fluorescent Protein (DsRed.T4 variant) gene and 2.1kb of sequence from the human growth hormone gene (to confer high expression of the DsRed.T4) under the control of the mouse insulin 1 promoter was injected into fertilized CD1 mouse eggs. Founder line 32 was subsequently established. Upon arrival at The Jackson Laboratory, transgenic mice were bred with C57BL/6J to establish this colony on a mixed genetic background.
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (169 strains)
006872 B6.Cg-Tg(Ins1-DsRed*T4)32Hara/J
006051 B6.Cg-Tg(CAG-DsRed*MST)1Nagy/J 008605 B6;C3-Tg(CAG-DsRed,-EGFP)5Gae/J 005328 NOD/ShiLt-Tg(Cd4-DsRed)4Lt/J 005438 STOCK Tg(CAG-Bgeo,-DsRed*MST)1Nagy/J 005441 STOCK Tg(CAG-DsRed*MST)1Nagy/J 008241 STOCK Tg(Cspg4-DsRed.T1)1Akik/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
Tg(Ins1-DsRed*T4)32Hara/0
involves: CD-1
- homeostasis/metabolism phenotype
- *normal* homeostasis/metabolism phenotype (MGI Ref ID J:120245)
- no differences in fasting blood glucose or body weight are observed compared to wild-type littermates
This mouse can be used to support research in many areas including:
DsRed relatedResearch Tools
Diabetes and Obesity Research
Fluorescent Proteins
Genetics Research (Tissue/Cell Markers: pancreatic beta cells)
Ins1 relatedResearch Tools
Fluorescent Proteins
Diabetes and Obesity Research
Type 1 Diabetes (IDDM) Analysis Strains (NOD/ShiLtJ Non-MHC Congenics)
Immunology and Inflammation Research
Autoimmunity (Type 1 Diabetes)
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Ins1-DsRed*T4)32Hara | ||
|---|---|---|---|
| Allele Name | transgene insertion 32, Manami Hara | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | MIP-RFP (line 12); MIP-RFP (line 32); | ||
| Mutation Made By | Manami Hara, University of Chicago | ||
| Strain of Origin | CD-1 | ||
| Site of Expression | DsRed.T4 expression is detected in tissues where insulin I is normally expressed; fluorescent protein expression in pancreatic beta-cells is evident from embryonic day (E)13.5 through adulthood. | ||
| Expressed Gene | DsRed, red fluorescent protein, | ||
| Promoter | Ins1, insulin I, mouse, laboratory | ||
| Molecular Note | The transgenic construct consists of the Red Fluorescent Protein (DsRed.T4 variant) gene and 2.1kb of sequence from the human growth hormone gene (to confer high expression of the DsRed.T4) under the control of the mouse insulin 1 promoter. Line 32 was subsequently established. Fluorescence is detected in tissues where insulin I is normally expressed; fluorescent protein expression in pancreatic beta-cells is evident from embryonic day (E)13.5 through adult. [MGI Ref ID J:120245] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(DsRed), QPCR, vers. 3
Tg(Ins1-DsRed*T4)32Hara, MCA, vers. 4
Tg(Ins1-DsRed*T4)32Hara, SEP PCR, vers. 2
Tg(Ins1-DsRed*T4)32Hara, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Hara M; Dizon RF; Glick BS; Lee CS; Kaestner KH; Piston DW; Bindokas VP. 2006. Imaging pancreatic beta-cells in the intact pancreas. Am J Physiol Endocrinol Metab 290(5):E1041-7. [PubMed: 16368785] [MGI Ref ID J:120245]
Additional References
Tg(Ins1-DsRed*T4)32Hara relatedHara M; Wang X; Kawamura T; Bindokas VP; Dizon RF; Alcoser SY; Magnuson MA; Bell GI. 2003. Transgenic mice with green fluorescent protein-labeled pancreatic beta -cells. Am J Physiol Endocrinol Metab 284(1):E177-83. [PubMed: 12388130] [MGI Ref ID J:99450]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, homozygous mice may be bred. Mating System Homozygote x Homozygote (Female x Male) Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
This strain is currently This strain is currently on HOLD - Contact Customer Service for more information..
To register your interest in this strain go to the Strain Interest Form.
Estimated Available for Sale Date: 22-OCT-07
Please note: Estimated available for sale dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for sale depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain/interest registered.
View All Strains Under Development and On Hold
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $291.90 Female or Male Homozygous for Tg(Ins1-DsRed*T4)32Hara *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $583.80 Homozygous for Tg(Ins1-DsRed*T4)32Hara x Homozygous for Tg(Ins1-DsRed*T4)32Hara
Additional Supply Details
| Pricing for International shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $379.50 Female or Male Homozygous for Tg(Ins1-DsRed*T4)32Hara *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $759.00 Homozygous for Tg(Ins1-DsRed*T4)32Hara x Homozygous for Tg(Ins1-DsRed*T4)32Hara
Additional Supply Details
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Supply Details
| Standard Supply | This strain is currently on HOLD - Contact Customer Service for more information. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
See Terms of Use
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