Strain Name: |
B6.Cg-Tg(Ins1-DsRed*T4)32Hara/J |
|---|---|
Stock Number: |
006872 |
Availability: | Repository- Live |
General Terms and Conditions |
| Genes & Alleles | DsRed; Ins1; Tg(Ins1-DsRed*T4)32Hara; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Mating System Noncarrier x Hemizygote (Female x Male) Species laboratory mouse Donating Investigator Manami Hara, University of Chicago Generation N4 (15-MAY-08) Strain Description
Mice hemizygous for this "MIP-RFP" transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express the DsRed.T4 variant of Enhanced Red Fluorescent Protein under the control of mouse insulin 1 promoter. Fluorescence is detected in tissues where insulin I is normally expressed; fluorescent protein expression in pancreatic beta-cells is evident from embryonic day (E)13.5 through adult. Transgenic mice exhibit normal glucose tolerance and pancreatic insulin levels. While the human growth hormone sequence in the transgene is designed to enhance expression of the fluorescent protein and is not expressed in a similarly constructed transgene (see Stock No. 006864), whether or not it is expressed for this strain was not characterized. This mutant mouse strain may be useful in studies of pancreatic development and pancreatic beta islet cell biology.In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Strain Development
A transgenic construct containing the Red Fluorescent Protein (DsRed.T4 variant) gene and 2.1kb of sequence from the human growth hormone gene (to confer high expression of the DsRed.T4) under the control of the mouse insulin 1 promoter was injected into fertilized CD1 mouse eggs. Founder line 32 was subsequently established. Upon arrival at The Jackson Laboratory, transgenic mice on this outbred background (see Stock No. 006866) were bred to C57BL/6J inbred mice for at least 5 generations to generate this congenic strain (Stock No. 006872).
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Tg(Ins1-DsRed*T4)32Hara | ||
|---|---|---|---|
| Allele Name | transgene insertion 32, Manami Hara | ||
| Common Name(s) | MIP-RFP (line 12); MIP-RFP (line 32); | ||
| Mutation Made By | Manami Hara, University of Chicago | ||
| Strain of Origin | CD-1 | ||
| Site of Expression | DsRed.T4 expression is detected in tissues where insulin I is normally expressed; fluorescent protein expression in pancreatic beta-cells is evident from embryonic day (E)13.5 through adulthood. | ||
| Expressed Gene | DsRed, red fluorescent protein, | ||
| Promoter | Ins1, insulin I, mouse, laboratory | ||
| Molecular Note | The transgenic construct consists of the Red Fluorescent Protein (DsRed.T4 variant) gene and 2.1kb of sequence from the human growth hormone gene (to confer high expression of the DsRed.T4) under the control of the mouse insulin 1 promoter. Line 32 was subsequently established. Fluorescence is detected in tissues where insulin I is normally expressed; fluorescent protein expression in pancreatic beta-cells is evident from embryonic day (E)13.5 through adult. [MGI Ref ID J:120245] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Tg(Ins1-DsRed*T4)32Hara
Tg(Ins1-DsRed*T4)32Hara
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, hemizygotes are bred to C57BL/6J inbred mice. The Donating Investigator reports that homozygous mice (on the original CD1 outbred background) are viable and fertile, but has not confirmed homozygosity. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (146 strains)
006866 STOCK Tg(Ins1-DsRed*T4)32Hara/J
Additional Web Information
Congenic Nomenclature
Fluorescent Proteins/lacZ Systems
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
DsRed relatedResearch Tools
Diabetes and Obesity Research
Fluorescent Proteins
Genetics Research (Tissue/Cell Markers: pancreatic beta cells)
Ins1 relatedResearch Tools
Fluorescent Proteins
Diabetes and Obesity Research
Type 1 Diabetes (IDDM) Analysis Strains (NOD/ShiLtJ Non-MHC Congenics)
Immunology and Inflammation Research
Autoimmunity (Type 1 Diabetes)
References
Selected Reference(s)
Additional ReferencesHara M; Dizon RF; Glick BS; Lee CS; Kaestner KH; Piston DW; Bindokas VP. 2006. Imaging pancreatic beta-cells in the intact pancreas. Am J Physiol Endocrinol Metab 290(5):E1041-7. [PubMed: 16368785] [MGI Ref ID J:120245]
Price and Supply Information
| Strain Name: | B6.Cg-Tg(Ins1-DsRed*T4)32Hara/J |
| Stock Number: | 006872 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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