Strain Name: |
B6.129-Gabra4tm1.2Geh/J |
|---|---|
Stock Number: |
006874 |
Availability: | Repository- Live |
General Terms and Conditions |
| Genes & Alleles | Gabra4; Gabra4tm1.2Geh; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Mating System Heterozygote x Heterozygote (Female x Male) Species laboratory mouse Donating Investigator Gregg Homanics, University of Pittsburgh Generation N3+ (10-OCT-07) Strain Description
Mice homozygous for this GABAA-R alpha4F allele are viable and fertile. These mutant mice have loxP sites flanking exon 3 of the targeted gene. When bred to Cre-recombinase expressing mice, offspring will have a deletion of exon 3 in the cre expressing tissue(s). These "floxed" mice may be useful in neurological studies including behavior and neurotransmitter function.Of note, several strains bearing gamma-aminobutyric acid (GABA-A) receptor mutations are available from this donating investigator (Dr. Gregg Homanics, University of Pittsburgh), including Gabra1 (Stock No. 004318), Gabra4 (Stock No. 006874), Gabra6 (Stock No. 002710), Gabrb3 (Stock No. 002711), Gabrd (Stock No. 003725), and Gabrg2 (Stock No. 003137).
Strain Development
A targeting vector was designed to place a loxP site upstream and a frt-flanked neomycin resistance gene and loxP site downstream of exon 3 of the targeted gene. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were microinjected into C57BL/6J blastocysts. Chimeric mice were bred to C57BL/6J females. Offspring with the "floxed" exon 3 allele and neomycin cassette (Fneo) were bred to actin-FLPe mice (on a mixed C57BL/6J and SJL genetic background). Offspring with "floxed" exon 3 and excised neo allele (F) were subsequently backcrossed to C57BL/6J for 2 generations. Mice were then backcrossed to an EIIa-Cre transgenic mouse strain (C57BL/6J congenic background, see Stock No. 003724). The resulting mice still containing the non-recombined "floxed" exon 3 (F) allele that were negative for the cre transgene were bred to C57BL/6J for two more generations prior to arrival at The Jackson Laboratory. Upon arrival, mutant mice were bred to C57BL/6J for at least three generations.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Gabra4tm1.2Geh | ||
|---|---|---|---|
| Allele Name | targeted mutation 1.2, Gregg E Homanics | ||
| Common Name(s) |
F;
GABAA-R alpha4 | ||
| Mutation Made By | Gregg Homanics, University of Pittsburgh | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ | ||
| ES Cell Line Name | R1 | ||
| ES Cell Line Strain | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Gene Symbol and Name | Gabra4, gamma-aminobutyric acid (GABA-A) receptor, subunit alpha 4 | ||
| Chromosome | 5 | ||
| Gene Common Name(s) | Gabra-4; | ||
| Molecular Note | Offspring with a floxed exon 3 allele and neomycin cassette (Fneo) were bred to actin-FLPe mice to remove the Neomycin cassette. [MGI Ref ID J:115154] | ||
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Gabra4tm1.2Geh
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, heterozygous or homozygous mice can be bred. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying other alleles of Gabra4
005812 B6.129P2-Gabra4tm1Dgen/J View Strains carrying other alleles of Gabra4 (1 strain)
Additional Web Information
Congenic Nomenclature
Cre-lox or FLP-FRT Systems
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
Neurobiology Research
Behavioral and Learning Defects
Cre-lox System (loxP-flanked Sequences)
Receptor Defects (GABA receptor)
Receptor Defects
Research Tools
Cre-lox System (loxP-flanked Sequences)
Neurobiology Research
References
Selected Reference(s)
Chandra D; Jia F; Liang J; Peng Z; Suryanarayanan A; Werner DF; Spigelman I; Houser CR; Olsen RW; Harrison NL; Homanics GE. 2006. GABAA receptor alpha 4 subunits mediate extrasynaptic inhibition in thalamus and dentate gyrus and the action of gaboxadol. Proc Natl Acad Sci U S A 103(41):15230-5. [PubMed: 17005728] [MGI Ref ID J:115154]
Price and Supply Information
| Strain Name: | B6.129-Gabra4tm1.2Geh/J |
| Stock Number: | 006874 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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