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Strain Name:

FVB/N-Tg(Tagln-rtTA)E1Jwst/J

Stock Number:

006875

Availability:

Repository- Live

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Additional Licensing and Use Restrictions

Genes & Alleles   Tagln;   rtTA;   Tg(Tagln-rtTA)E1Jwst;

Product Information

Strain Details

Type JAX® GEMM® Strain - Coisogenic
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Mutant Strain
Type JAX® GEMM® Strain - Transgenic
Mating SystemHemizygote x Hemizygote         (Female x Male)
Specieslaboratory mouse
Donating Investigator James West,   Univ. of Colorado Health Sciences Center
Generation?+F3 (28-APR-08)

Strain Description
Transgenic SM22-rtTA mice are viable and fertile. These mice express the reverse tetracycline-controlled transactivator (rtTA) protein under the control of the murine SM22-alpha (SM22a or transgelin) promoter. When hemizygotes are mated to a second transgenic strain carrying a gene of interest under the regulatory control of a tetracycline-responsive promoter element (TRE; tetO), expression of the target gene in the bitransgenic offspring is inducible in smooth muscle cells with administration of the tetracycline analog, doxycycline. These SM22-rtTA mice provide a "Tet-On" tool that allows the inducible expression of genes in smooth muscle cells.

Strain Development
The SM22-rtTA transgene contains a 488-bp fragment of the murine smooth muscle-specific SM22-alpha (or transgelin) promoter that drives expression of the reverse tetracycline transactivator gene (rtTA). The transgene was injected into FVB/N embryos. Transgenic mice (founder line E) on the FVB/N genetic background were bred together for many generations prior to arrival at The Jackson Laboratory.

Gene & Allele Details

Allele Symbol Tg(Tagln-rtTA)E1Jwst
Allele Name transgene insertion E1, James West
Common Name(s) SM22-rtTA;
Mutation Made By James West,   Univ. of Colorado Health Sciences Center
Strain of OriginFVB/N
Site of ExpressionExpresses rtTA in vascular smooth muscle.
Expressed Gene rtTA, reverse tetracycline-controlled transactivator, E. coli
The tetracycline repressor gene (Tetr), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). One mutant with a four amino acid residue change (rTetR) exhibited dependence on tetracycline for induction of the targeted gene and was used in the rtTA construct (Gossen et al, 1995). rTetr was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16).
Promoter Tagln, transgelin, mouse, laboratory
Molecular Note The transgene has the 488-bp fragment of the murine smooth muscle-specific SM22-alpha (Tagln or transgelin) promoter to drive expression of the reverse tetracycline transactivator gene (rtTA). [MGI Ref ID J:98898]

Control Information

  Control
   001800 FVB/NJ
 
  Considerations for Choosing Controls

Genotyping Protocols

Tg(tTA)
Tg(tTA) QPCR

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, homozygous mice may be bred together.
Diet Information LabDiet® 5K52/5K67

Related Strains

Strains carrying other alleles of Tagln
006878   B6.129S6-Taglntm2(cre)Yec/J
008082   B6;SJL-Tg(Tagln-tTA)1Mrab Tg(tetO-Mcpt1)1Mrab/J
004746   STOCK Tg(Tagln-cre)1Her/J
View Strains carrying other alleles of Tagln     (3 strains)

View Strains carrying other alleles of rtTA     (17 strains)

Additional Web Information

Tet Expression Systems

Animal Health Reports

Room Number           AX11

Research Applications

This mouse can be used to support research in many areas including:

Internal/Organ Research
Other

Research Tools
Cancer Research (Tetop Tet System)
Cardiovascular Research (Tetop Tet System)
Internal/Organ Research
Reproductive Biology Research (Tetop Tet System)
Tet Expression Systems (tTA/rtTA Expressing Strains)

References

Selected Reference(s)

West J; Fagan K; Steudel W; Fouty B; Lane K; Harral J; Hoedt-Miller M; Tada Y; Ozimek J; Tuder R; Rodman DM. 2004. Pulmonary hypertension in transgenic mice expressing a dominant-negative BMPRII gene in smooth muscle. Circ Res 94(8):1109-14. [PubMed: 15031260]  [MGI Ref ID J:98898]

Additional References

Price and Supply Information

Strain Name: FVB/N-Tg(Tagln-rtTA)E1Jwst/J
Stock Number: 006875

Price Details

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Supply Details

Standard SupplyRepository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes Usually shipped between four and eight weeks of age.
This strain is included in the Induced Mutant Resource Colony collection.
LicensingSee General Terms and Conditions below for Licensing and Use Restrictions  
Control InformationView Control Information in Strain Details.

General Terms and Conditions

View JAX® Mice & Services Conditions of Use.

Use of the Tet-System may require a license, see Licenses for Strains Using TET-System Technology.

For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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