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| These SM22-rtTA mice provide a Tet-On tool that allows the inducible expression of genes in smooth muscle cells. | |||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
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Description
Strain Information
Type Coisogenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Hemizygote x Hemizygote (Female x Male) 02-AUG-07 Species laboratory mouse Generation ?+F4 (08-JAN-09) Donating Investigator James West, Univ. of Colorado Health Sciences Center Description
Transgenic SM22-rtTA mice are viable and fertile. These mice express the reverse tetracycline-controlled transactivator (rtTA) protein under the control of the murine SM22-alpha (SM22α or transgelin) promoter. When hemizygotes are mated to a second transgenic strain carrying a gene of interest under the regulatory control of a tetracycline-responsive promoter element (TRE; tetO), expression of the target gene in the bitransgenic offspring is inducible in smooth muscle cells with administration of the tetracycline analog, doxycycline. These SM22-rtTA mice provide a "Tet-On" tool that allows the inducible expression of genes in smooth muscle cells.Development
The SM22-rtTA transgene contains a 488-bp fragment of the murine smooth muscle-specific SM22-alpha (or transgelin) promoter that drives expression of the reverse tetracycline transactivator gene (rtTA). The transgene was injected into FVB/N embryos. Transgenic mice (founder line E) on the FVB/N genetic background were bred together for many generations prior to arrival at The Jackson Laboratory.
Control Information
| Control | ||
|---|---|---|
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Tagln
006878 B6.129S6-Taglntm2(cre)Yec/J 008082 B6;SJL-Tg(Tagln-tTA)1Mrab Tg(tetO-Mcpt1)1Mrab/J 004746 STOCK Tg(Tagln-cre)1Her/J View Strains carrying other alleles of Tagln (3 strains)
006965 B6.Cg-Gt(ROSA)26Sortm1(rtTA*M2)Jae/J 005670 B6.Cg-Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J 007176 B6.Cg-Tg(Pax8-rtTA2S*M2)1Koes/J 006235 B6.Cg-Tg(SFTPC-rtTA)5Jaw/J 006232 B6.Cg-Tg(Scgb1a1-rtTA)1Jaw/J 006911 B6;129-Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm2(tetO-Pou5f1)Jae/J 007678 B6;SJL-Tg(KRT14-rtTA)208Jek/J 010576 B6;SJL-Tg(MMTV-rtTA)4-1Jek/J 006245 C.Cg-Tg(SFTPC-rtTA)5Jaw/J 006242 C.Cg-Tg(Scgb1a1-rtTA)1Jaw/J 008099 FVB-Tg(KRT14-rtTA)F42Efu/J 004127 FVB-Tg(Nes-rtTA)306Rvs/J 006225 FVB.Cg-Tg(SFTPC-rtTA)5Jaw/J 006222 FVB.Cg-Tg(Scgb1a1-rtTA)1Jaw/J 008202 FVB/N-Tg(NPHS2-rtTA2*M2)1Jbk/J 004602 NOD.Cg-Tg(Ins2-rtTA)2Doi/DoiJ 005734 NOD/Lt-Tg(Ins2-rtTA)1Ach/AchJ 005572 STOCK Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J 008755 STOCK Tg(Ins2-rtTA)2Efr Tg(teto-DTA)1Gfi/J 008250 STOCK Tg(Ins2-rtTA)2Efr/J 005493 STOCK Tg(Tek-rtTA,TRE-lacZ)1425Tpr/J 003273 STOCK Tg(rtTAhCMV)4Bjd/J
Additional Web Information
Tet Expression Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Internal/Organ Research
Other
Research Tools
Cancer Research
Tetop Tet System
Cardiovascular Research
Tetop Tet System
Internal/Organ Research
Reproductive Biology Research
Tetop Tet System
Tet Expression Systems
tTA/rtTA Expressing Strains
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Tagln-rtTA)E1Jwst | ||
|---|---|---|---|
| Allele Name | transgene insertion E1, James West | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | SM22-rtTA; | ||
| Mutation Made By | James West, Univ. of Colorado Health Sciences Center | ||
| Strain of Origin | FVB/N | ||
| Site of Expression | Expresses rtTA in vascular smooth muscle. | ||
| Expressed Gene | rtTA, reverse tetracycline-controlled transactivator, E. coli | ||
| The tetracycline repressor gene (Tetr), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). One mutant with a four amino acid residue change (rTetR) exhibited dependence on tetracycline for induction of the targeted gene and was used in the rtTA construct (Gossen et al, 1995). rTetr was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16). | |||
| Promoter | Tagln, transgelin, mouse, laboratory | ||
| Molecular Note | The transgene has the 488-bp fragment of the murine smooth muscle-specific SM22-alpha (Tagln or transgelin) promoter to drive expression of the reverse tetracycline transactivator gene (rtTA). [MGI Ref ID J:98898] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(tTA), Melt Curve Analysis
Tg(tTA), QPCR
Tg(tTA), Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
West J; Fagan K; Steudel W; Fouty B; Lane K; Harral J; Hoedt-Miller M; Tada Y; Ozimek J; Tuder R; Rodman DM. 2004. Pulmonary hypertension in transgenic mice expressing a dominant-negative BMPRII gene in smooth muscle. Circ Res 94(8):1109-14. [PubMed: 15031260] [MGI Ref ID J:98898]
Additional References
Tg(Tagln-rtTA)E1Jwst relatedHagen M; Fagan K; Steudel W; Carr M; Lane K; Rodman DM; West J. 2007. Interaction of interleukin-6 and the BMP pathway in pulmonary smooth muscle. Am J Physiol Lung Cell Mol Physiol 292(6):L1473-9. [PubMed: 17322283] [MGI Ref ID J:125848]
Tada Y; Majka S; Carr M; Harral J; Crona D; Kuriyama T; West J. 2007. Molecular effects of loss of BMPR2 signaling in smooth muscle in a transgenic mouse model of PAH. Am J Physiol Lung Cell Mol Physiol 292(6):L1556-63. [PubMed: 17369292] [MGI Ref ID J:125877]
West J; Harral J; Lane K; Deng Y; Ickes B; Crona D; Albu S; Stewart D; Fagan K. 2008. Mice expressing BMPR2R899X transgene in smooth muscle develop pulmonary vascular lesions. Am J Physiol Lung Cell Mol Physiol 295(5):L744-55. [PubMed: 18723761] [MGI Ref ID J:142266]
Young KA; Ivester C; West J; Carr M; Rodman DM. 2006. BMP signaling controls PASMC KV channel expression in vitro and in vivo. Am J Physiol Lung Cell Mol Physiol 290(5):L841-8. [PubMed: 16339782] [MGI Ref ID J:115767]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, homozygous mice may be bred together. Mating System Hemizygote x Hemizygote (Female x Male) 02-AUG-07 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $209.90 Female or Male Homozygous for Tg(Tagln-rtTA)E1Jwst
Pairs /Price (US dollars $) Pair Genotype $419.80 Homozygous for Tg(Tagln-rtTA)E1Jwst x Homozygous for Tg(Tagln-rtTA)E1Jwst
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $272.90 Female or Male Homozygous for Tg(Tagln-rtTA)E1Jwst
Pairs /Price (US dollars $) Pair Genotype $545.80 Homozygous for Tg(Tagln-rtTA)E1Jwst x Homozygous for Tg(Tagln-rtTA)E1Jwst
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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Use of the Tet-System may require a license, see Licenses for Strains Using TET-System Technology.
For additional Licensing and Use Restrictions view the link(s) below:
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