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| These mice harbor a Cre-conditional TEL-AML1 (or iZ/EG-TEL-AML1) transgene and have high and widespread expression of lacZ under the control of the CMV enhancer/chicken actin promoter prior to Cre-mediated excision of the upstream "floxed" STOP sequence. When bred to mice expressing Cre recombinase, the STOP sequence is removed in the resulting offspring, allowing transcription/co-expression of both the human TEL-AML1 fusion protein and EGFP in all cre-expressing cells. These iZ/EG-TEL-AML1 mice may be useful for global expression of lacZ or, when crossed to a cre-expressing strain, for studying hematopoietic development, cancer, and leukemia. | |||||||||||
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Description
Strain Information
Former Names STOCK Tg(ACTB-Bgeo,-TEL/AML1,-EGFP)A6Lbe/J (Changed: 08-MAY-08 ) STOCK Tg(ACTB-Bgeo,TEL/AML1,EGFP)A6Lbe/J (Changed: 19-APR-07 ) Type Mutant Stock; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Noncarrier x Hemizygote (Female x Male) Species laboratory mouse Generation N1F4 (22-DEC-08) Donating Investigator Corrinne Lobe, Sunnybrook & Women's College HSC Description
Mice hemizygous for this Cre-conditional TEL-AML1 (or iZ/EG-TEL-AML1) transgene are viable and fertile. Homozygotes die in utero, presumably due to high lacZ expression. Prior to Cre-mediated excision of the "floxed" STOP sequence, high expression of lacZ is observed in cells and tissues. When bred to Cre recombinase transgenic mice, the STOP sequence (and beta-geo) is removed in the resulting offspring, allowing transcription/co-expression of both the human TEL-AML1 fusion protein and EGFP in all cre-expressing cells. TEL-AML1 transcripts are not observed in adult organ tissues prior to excision of the floxed sequences. Following Cre-mediated deletion of the STOP sequence (by B6.Cg-Tg(Tek-cre)12Flv/J, Stock No. 004128), Western blot analysis reveals that EGFP levels are well correlated with TEL-AML1 transcript levels. While global expression of TEL-AML1 leads to embryonic lethality (E7.5), hematopoietic and endothelial expression leads to malignancy in B- and T- lymphoid cells and secondary mutations that closely resemble human TEL-AML1 associated leukemia. These transgenic mice may be useful for global expression of lacZ or, when crossed to a cre-expressing strain, for studying hematopoietic development, cancer, and leukemia.Development
The iZ/EG-TEL-AML1 transgenic construct contains the CMV enhancer/chicken beta-actin promoter, a loxP-flanked STOP sequence (beta-geo reporter followed by SV40 polyadenylation signal), the human TEL-AML1 fusion protein sequence (coding for the translocation t(12;21)(p13;q22)), an internal ribosomal entry site, and an enhanced green fluorescent protein (EGFP) gene. This construct was electroporated into 129X1/SvJ x 129S1/Sv-derived R1 embryonic stem (ES) cells. ES cells with strong lacZ expression, single copy transgene integration, and efficient expression of EGFP following Cre-mediated excision of the STOP sequence were selected and aggregated with E2.5 8-cell CD1 mouse embryos. Following transfer into pseudo-pregnant females, chimeric males were mated to wild-type CD1 females to establish founder line A6. This strain was maintained by breeding hemizygous mice to CD1 or wild-type siblings for approximately six generations prior to arrival at The Jackson Laboratory. Upon arrival, these mice were bred at least one generation to C57BL/6J.
Control Information
| Control | ||
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| Noncarrier | ||
| Considerations for Choosing Controls | ||
Related Strains
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Additional Web Information
Cre-lox Systems
Fluorescent Proteins/lacZ Systems
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(CAG-Bgeo,-TEL/AML1,-EGFP)A6Lbe | ||
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| Allele Name | transgene insertion A6, Corinne G Lobe | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | Tg(ACTB-Bgeo,-TEL/AML1,-EGFP)A6Lbe; Tg(CAG-Bgeo,-TEL/AML1,-EGFP)A6Lbe; iZ/EG-TEL-AML1; | ||
| Mutation Made By | Corrinne Lobe, Sunnybrook & Women's College HSC | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| ES Cell Line Name | R1 | ||
| ES Cell Line Strain | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Site of Expression | lacZ is highly expressed in most cells and tissues. When bred to Cre recombinase expressing mice, lacZ expression is replaced with co-expression of human TEL-AML1 fusion protein and EGFP in Cre recombinase expressing cells. | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Expressed Gene | Bgeo, fusion of beta-galactosidase and neomycin phosphotransferase genes , E. coli | ||
| Promoter | ACTB, actin, beta, chicken | ||
| Molecular Note | A transgenic construct was designed with the CMV enhancer/chicken beta-actin promoter, loxP-flanked STOP sequence (beta-geo reporter followed by SV40 polyadenylation signal), human TEL-AML1 fusion protein sequence (coding for the translocation t(12;21)(p13;q22)), internal ribosomal entry site, and an enhanced green fluorescent protein (EGFP) gene. Prior to Cre-mediated excision of the "floxed" STOP sequence, high expression of lacZ is observed in cells and tissues. When bred to Cre recombinase expressing mice, the STOP sequence (and beta-geo) is removed in the resulting offspring, allowing transcription/co-expression of both the human TEL-AML1 fusion protein and EGFP to proceed in the Cre recombinase expressing cells. [MGI Ref ID J:119130] | ||
| Gene Symbol and Name | Tg(CAG-Bgeo,-TEL/AML1,-EGFP)A6Lbe, transgene insertion A6, Corinne G Lobe | ||
| Chromosome | UN | ||
| Gene Common Name(s) | Tg(ACTB-Bgeo,-TEL/AML1,-EGFP)A6Lbe; iZ/EG-TEL-AML1; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(CAG-Bgeo,-TEL/AML1,-EGFP)A6Lbe, STD PCR, vers. 1
Helpful Links
Genotyping resources and troubleshooting
References
References
Additional References
Novak A; Guo C; Yang W; Nagy A; Lobe CG. 2000. Z/EG, a double reporter mouse line that expresses enhanced green fluorescent protein upon Cre-mediated excision. Genesis 28(3-4):147-55. [PubMed: 11105057] [MGI Ref ID J:83045]
Tg(CAG-Bgeo,-TEL/AML1,-EGFP)A6Lbe relatedYang W; Lobe CG. 2007. TEL-AML1 transgene expression in hematopoietic cells produces lymphoblastic leukemia in mice MGI Direct Data Submission :. [MGI Ref ID J:119130]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number FGB29
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, hemizygotes are bred to wildtype siblings. Homozygous mice die in utero presumably due to high lacZ expression. Mating System Noncarrier x Hemizygote (Female x Male) Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $243.50 Female or Male Hemizygous for Tg(CAG-Bgeo,-TEL/AML1,-EGFP)A6Lbe
Pairs /Price (US dollars $) Pair Genotype $297.85 Hemizygous for Tg(CAG-Bgeo,-TEL/AML1,-EGFP)A6Lbe x Noncarrier $297.85 Noncarrier x Hemizygous for Tg(CAG-Bgeo,-TEL/AML1,-EGFP)A6Lbe
Additional Supply Details
| Pricing for International shipping destinations |
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Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $316.60 Female or Male Hemizygous for Tg(CAG-Bgeo,-TEL/AML1,-EGFP)A6Lbe
Pairs /Price (US dollars $) Pair Genotype $387.30 Hemizygous for Tg(CAG-Bgeo,-TEL/AML1,-EGFP)A6Lbe x Noncarrier $387.30 Noncarrier x Hemizygous for Tg(CAG-Bgeo,-TEL/AML1,-EGFP)A6Lbe
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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