Go to JAX® Mice Query Form

Strain Name:

B6.Cg-Ldlrtm1Her Tg(H2-K-AKR1B1)1Tj/J

Stock Number:

006877

Availability:

Repository- Live

View Pricing for USA, Canada and Mexico
View Pricing for International

General Terms and Conditions

Genes & Alleles   AKR1B1;   H2-K;   Ldlr;   Ldlrtm1Her;   Tg(H2-K-AKR1B1)1Tj;

Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Mutant Strain
Type JAX® GEMM® Strain - Targeted Mutation
Type JAX® GEMM® Strain - Transgenic
Specieslaboratory mouse
Donating Investigator Jan Breslow,   Rockefeller University
GenerationN7+N2 (08-JAN-08)

Strain Description
Independently, mice hemizygous for this "huAR" transgene express human aldose reductase as a model for increased oxidative stress, while LDLR homozygotes are predisposed to atherosclerosis and hypercholesterolemia. The donating investigators report that the H2-Kd promoter functions on this H2-Kb genetic background without any loss of transgene expression. When mutant mice are hemizygous for the transgene and homozygous for the targeted allele, they may be useful in studies of diabetes, metabolism, atherosclerosis, hypercholesterolemia, and oxidative stress.

Strain Development
The Ldlrtm1Her mutation was made by Dr. Robert Hammer and Joachim Herz (HHMI, University of Texas Southwestern Medical Center). Briefly, a targeting vector was used to insert a neo cassette into exon 4. The vector was electroporated into 129S7/SvEvBrd-derived AB1 embryonic stem (ES) cells. Chimeric mice were bred to C57BL/6J, and the strain was made congenic on a C57BL/6J genetic background at The Jackson Laboratory (Stock No. 002207). The "huAR" transgene was created by Dr. Yamaoka and colleagues (University of Tokushima) by placing a human aldose reductase (AKR1B1) cDNA under the control of the murine H2-Kd MHC class I promoter. A single male was used to generate transgenic mice on a mixed C57BL/6;DBA/2 genetic background. Transgenic mice were then sent to Dr. Eva Feldman (University of Michigan). From there, mice on a mixed (approximately 80% C57BL/6J) background were shipped to Dr. Jan Breslow at The Rockefeller University. To generate the double mutant strain, Stock No. 002207 mice were bred with the huAR transgenic mice in the laboratory of Dr. Breslow. Double mutant mice were then backcrossed to C57BL/6J for approximately 7 generations prior to arrival at The Jackson Laboratory. The donating investigators indicate that 103/104 microsatellite markers indicate C57BL/6J background. The si ngle exception is D5Mit314. The Y chromosome may not have been fixed to the C57BL/6J genetic background.

Gene & Allele Details

Allele Symbol Ldlrtm1Her
Allele Name targeted mutation 1, Joachim Herz
Common Name(s) LDLR KO; LDLR-; LDLr0; LDLrKO; Ldlrtm1Her;
Mutation Made By Joachim Herz,   Univ of Texas Southwest Med Ctr Dallas
Strain of Origin129S7/SvEvBrd-Hprt1<+>
ES Cell Line NameAB1
ES Cell Line Strain129S7/SvEvBrd-Hprt1<+>
Gene Symbol and Name Ldlr, low density lipoprotein receptor
Chromosome 9
Gene Common Name(s) FH; FHC; LDLRA;
General Note When used in bone marrow transplant into Ldlrtm1Her homozygous mice, Abca1tm1Jdm Abcg1tm1Dgen homozygous cells accelerate the development of atherosclerosis. (J:130777)
Molecular Note Insertion of a neomycin resistance cassette into exon 4. The authors predict that the targeted allele would encode a truncated non-functional protein that will not bind LDL, and that lacks a membrane spanning segment. Immunoblot analysis of liver membranes detected a truncated protein in homozygous mutant animals. [MGI Ref ID J:37394]
 
Allele Symbol Tg(H2-K-AKR1B1)1Tj
Allele Name transgene insertion 1, University of Tokushima School of Medicine
Common Name(s) hAR-Tg;
Strain of Origin(C57BL/6 x DBA/2)F1
Expressed Gene AKR1B1, aldo-keto reductase family 1, member B1 (aldose reductase), human
Promoter H2-K, histocompatibility 2, K region, mouse, laboratory
Molecular Note Expression of the full length human aldose reductase (AKR1B1) cDNA is under control of the murine H2-Kd promoter. Northern and Wester blotting shows expression of human aldose reductase mRNA and protein in all tissues tested. [MGI Ref ID J:120049]

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Genotyping Protocols

Ldlr tm1Her
Tg(H2-K-AKR1B1)1Tj

Colony Maintenance

Diet Information LabDiet® 5K52/5K67

Related Strains

View Strains carrying   Ldlrtm1Her     (11 strains)

View Strains carrying other alleles of H2-K     (9 strains)

Strains carrying other alleles of Ldlr
005061   C57BL/6J-LdlrHlb301/J
View Strains carrying other alleles of Ldlr     (1 strain)

Additional Web Information

Congenic Nomenclature

Animal Health Reports

Room Number           FGB29

Research Applications

This mouse can be used to support research in many areas including:

Cardiovascular Research
Atherosclerosis
Hypercholesterolemia
Other (altered fat metabolism)

Diabetes and Obesity Research
Hyperglycemia

Metabolism Research
Lipid Metabolism

Research Tools
Cardiovascular Research
Diabetes and Obesity Research
Metabolism Research

Ldlrtm1Her related

Cardiovascular Research
Atherosclerosis
Hypercholesterolemia

Metabolism Research
Lipid Metabolism

Mouse/Human Gene Homologs
hypercholesterolemia, familial

References

Additional References

Price and Supply Information

Strain Name: B6.Cg-Ldlrtm1Her Tg(H2-K-AKR1B1)1Tj/J
Stock Number: 006877

Price Details

IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.

*NO Shipping Destination selected!

 

Supply Details

Standard SupplyRepository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes Usually shipped between four and eight weeks of age.
This strain is included in the Induced Mutant Resource Colony collection.
LicensingSee General Terms and Conditions below  
Control InformationView Control Information in Strain Details.

General Terms and Conditions

View JAX® Mice & Services Conditions of Use.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering and Purchasing Information

      Purchasing Information
      JAX® Mice Orders
      Surgical Services

Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Go to JAX® Mice Query Form

(2.15)