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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Heterozygote x Homozygote (Female x Male) 10-JUL-08 Species laboratory mouse Generation N12+N1F4 (18-AUG-09) Donating Investigator Yuqing Chen, University of Michigan, Medical School Description
Mice homozygous for this SM22alpha-CreKI allele are viable and fertile. These mice have a Cre-recombinase gene inserted into the endogenous transgelin (SM22alpha) locus. The donating investigator reports that this mutation results in a loss of function of the targeted gene. Cre recombinase activity is shown in adult smooth muscle cells (such as arteries, veins, and visceral organs) and cardiac myocytes, but activity is not observed in the same embryonic tissues. It has been the experience of The Jackson Laboratory that optimal breeding is achieved by mating heterozygous females to homozygous males as female mortality post gestation has been noted in our colony. These SM22alpha-CreKI mice may be useful for Cre-lox technology applications in studying smooth muscle and cardiac gene function, as well as cardiovascular disease.Development
A targeting vector was designed to insert a Cre-recombinase coding sequence (followed by a PGK-neo cassette) in-frame at the SM22alpha (Tagln or transgelin) initiation site. The construct was electroporated into 129S6/SvEvTac-derived "CMT-1" embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Chimeric animals were crossed with C57BL/6 to generate heterozygotes. This strain was backcrossed to C57BL/6J for at least 12 generations prior to arrival at The Jackson Laboratory. The Y chromosome may not have been fixed to the C57BL/6J genetic background.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Tagln
008082 B6;SJL-Tg(Tagln-tTA)1Mrab Tg(tetO-Mcpt1)1Mrab/J 006875 FVB/N-Tg(Tagln-rtTA)E1Jwst/J 004746 STOCK Tg(Tagln-cre)1Her/J View Strains carrying other alleles of Tagln (3 strains)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Taglntm2(cre)Yec/Taglntm2(cre)Yec
involves: 129S6/SvEvTac * C57BL/6
- normal phenotype
- no abnormal phenotype detected (MGI Ref ID J:119865)
- homozygous and heterozygous mice are viable, fertile, and phenotypically normal; mice show cre expression in all vascular smooth muscle cells of all arteries and veins examined and in bladder and GI tract
This mouse can be used to support research in many areas including:
cre relatedCardiovascular Research
Other
Research Tools
Cardiovascular Research
Cre-lox System
Cre-lox System
Cre Recombinase Expression: Germline/Embryonic Expression
Developmental Biology Research
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Tissue/Cell Markers: Cre-lox System
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Taglntm2(cre)Yec | ||
|---|---|---|---|
| Allele Name | targeted mutation 2, Yuqing E Chen | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | SM22alpha-creKI; Taglntm2(cre)Liw; | ||
| Mutation Made By | Jifeng Zhang, University of Michigan, Medical School | ||
| Strain of Origin | 129S6/SvEvTac | ||
| ES Cell Line Strain | 129 | ||
| Site of Expression | adult smooth muscle cells (such as arteries, veins, and visceral organs) and cardiac myocytes | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Gene Symbol and Name | Tagln, transgelin | ||
| Chromosome | 9 | ||
| Gene Common Name(s) | DKFZp686B01212; DKFZp686P11128; SM-22 alpha; SM22; SMCC; Sm22a; TAGLN1; WS3-10; smooth muscle protein, alpha, 22 kDa; | ||
| Driver Note | Tagln | ||
| Molecular Note | The cre recombinase coding sequence was inserted in frame into the initiation site, as well as a PGK-neo. [MGI Ref ID J:119865] | ||
Genotyping
Genotyping Information
Genotyping Protocols
TagIntm2(cre)Yec, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Zhang J; Zhong W; Cui T; Yang M; Hu X; Xu K; Xie C; Xue C; Gibbons GH; Liu C; Li L; Chen YE. 2006. Generation of an adult smooth muscle cell-targeted Cre recombinase mouse model. Arterioscler Thromb Vasc Biol 26(3):e23-4. [PubMed: 16484601] [MGI Ref ID J:119865]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, it has been the experience of The Jackson Laboratory that optimal breeding is achieved by mating heterozygous females to homozygous males. Female mortality post gestation has been noted in our colony. Mating System Heterozygote x Homozygote (Female x Male) 10-JUL-08 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $300.70 Female or Male Heterozygous for Taglntm2(cre)Yec $300.70 Female or Male Homozygous for Taglntm2(cre)Yec
Pairs /Price (US dollars $) Pair Genotype $601.40 Heterozygous for Taglntm2(cre)Yec x Homozygous for Taglntm2(cre)Yec
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $391.00 Female or Male Heterozygous for Taglntm2(cre)Yec $391.00 Female or Male Homozygous for Taglntm2(cre)Yec
Pairs /Price (US dollars $) Pair Genotype $781.90 Heterozygous for Taglntm2(cre)Yec x Homozygous for Taglntm2(cre)Yec
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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Tel: 1-800-422-6423 or 1-207-288-5845
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Terms of Use
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For Licensing and Use Restrictions view the link(s) below:
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Contact information
General inquiries
Contracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
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