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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Heterozygote x Homozygote (Female x Male) 10-JUL-08 Species laboratory mouse Generation N12+N1F8 (14-MAR-11)
Generation DefinitionsDonating Investigator Yuqing Chen, University of Michigan, Medical School Description
Mice homozygous for this SM22alpha-CreKI allele are viable and fertile. These mice have a Cre-recombinase gene inserted into the endogenous transgelin (SM22alpha) locus. The donating investigator reports that this mutation results in a loss of function of the targeted gene. Cre recombinase activity is shown in adult smooth muscle cells (such as arteries, veins, and visceral organs) and cardiac myocytes, but activity is not observed in the same embryonic tissues. It has been the experience of The Jackson Laboratory that optimal breeding is achieved by mating heterozygous females to homozygous males as female mortality post gestation has been noted in our colony. These SM22alpha-CreKI mice may be useful for Cre-lox technology applications in studying smooth muscle and cardiac gene function, as well as cardiovascular disease.Development
A targeting vector was designed to insert a Cre-recombinase coding sequence (followed by a PGK-neo cassette) in-frame at the SM22alpha (Tagln or transgelin) initiation site. The construct was electroporated into 129S6/SvEvTac-derived "CMT-1" embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Chimeric animals were crossed with C57BL/6 to generate heterozygotes. This strain was backcrossed to C57BL/6J for at least 12 generations prior to arrival at The Jackson Laboratory. The Y chromosome may not have been fixed to the C57BL/6J genetic background.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Tagln
017491 B6.Cg-Tg(Tagln-cre)1Her/J 008082 B6;SJL-Tg(Tagln-tTA)1Mrab Tg(tetO-Mcpt1)1Mrab/J 006875 FVB/N-Tg(Tagln-rtTA)E1Jwst/J 004746 STOCK Tg(Tagln-cre)1Her/J View Strains carrying other alleles of Tagln (4 strains)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Taglntm2(cre)Yec/Taglntm2(cre)Yec
involves: 129S6/SvEvTac * C57BL/6
- normal phenotype
- no abnormal phenotype detected
- homozygous and heterozygous mice are viable, fertile, and phenotypically normal; mice show cre expression in all vascular smooth muscle cells of all arteries and veins examined and in bladder and GI tract (MGI Ref ID J:119865)
This mouse can be used to support research in many areas including:
cre relatedCardiovascular Research
Other
Research Tools
Cardiovascular Research
Cre-lox System
Cre-lox System
Cre Recombinase Expression
Cre Recombinase Expression: Germline/Embryonic Expression
Developmental Biology Research
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Tissue/Cell Markers
Tissue/Cell Markers: Cre-lox System
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Taglntm2(cre)Yec | ||
|---|---|---|---|
| Allele Name | targeted mutation 2, Yuqing E Chen | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | SM22alpha-creKI; Taglntm2(cre)Liw; | ||
| Mutation Made By | Jifeng Zhang, University of Michigan, Medical School | ||
| Strain of Origin | 129S6/SvEvTac | ||
| ES Cell Line Strain | 129 | ||
| Site of Expression | adult smooth muscle cells (such as arteries, veins, and visceral organs) and cardiac myocytes | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Driver Note | Tagln | ||
| Molecular Note | The cre recombinase coding sequence was inserted in frame into the initiation site, as well as a PGK-neo. [MGI Ref ID J:119865] | ||
| Gene Symbol and Name | Tagln, transgelin | ||
| Chromosome | 9 | ||
| Gene Common Name(s) | SM-22 alpha; SM22; SMCC; Sm22a; TAGLN1; WS3-10; smooth muscle protein, alpha, 22 kDa; | ||
Genotyping
Genotyping Information
Genotyping Protocols
TagIntm2(cre)Yec, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Zhang J; Zhong W; Cui T; Yang M; Hu X; Xu K; Xie C; Xue C; Gibbons GH; Liu C; Li L; Chen YE. 2006. Generation of an adult smooth muscle cell-targeted Cre recombinase mouse model. Arterioscler Thromb Vasc Biol 26(3):e23-4. [PubMed: 16484601] [MGI Ref ID J:119865]
Additional References
Taglntm2(cre)Yec relatedChang L; Villacorta L; Zhang J; Garcia-Barrio MT; Yang K; Hamblin M; Whitesall SE; D'Alecy LG; Chen YE. 2009. Vascular smooth muscle cell-selective peroxisome proliferator-activated receptor-gamma deletion leads to hypotension. Circulation 119(16):2161-9. [PubMed: 19364979] [MGI Ref ID J:166495]
He Y; Zhang H; Yu L; Gunel M; Boggon TJ; Chen H; Min W. 2010. Stabilization of VEGFR2 signaling by cerebral cavernous malformation 3 is critical for vascular development. Sci Signal 3(116):ra26. [PubMed: 20371769] [MGI Ref ID J:171969]
Lopes M; Goupille O; Cloment CS; Lallemand Y; Cumano A; Robert B. 2011. Msx genes define a population of mural cell precursors required for head blood vessel maturation. Development 138(14):3055-66. [PubMed: 21693521] [MGI Ref ID J:173525]
Yoshida T; Gan Q; Franke AS; Ho R; Zhang J; Chen YE; Hayashi M; Majesky MW; Somlyo AV; Owens GK. 2010. Smooth and cardiac muscle-selective knock-out of Kruppel-like factor 4 causes postnatal death and growth retardation. J Biol Chem 285(27):21175-84. [PubMed: 20439457] [MGI Ref ID J:165929]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, it has been the experience of The Jackson Laboratory that optimal breeding is achieved by mating heterozygous females to homozygous males. Female mortality post gestation has been noted in our colony. Mating System Heterozygote x Homozygote (Female x Male) 10-JUL-08 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $261.00 Female or Male Heterozygous for Taglntm2(cre)Yec $320.00 Female or Male Homozygous for Taglntm2(cre)Yec
Pairs /Price (US dollars $) Pair Genotype $581.00 Heterozygous for Taglntm2(cre)Yec x Homozygous for Taglntm2(cre)Yec Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $339.30 Female or Male Heterozygous for Taglntm2(cre)Yec $416.00 Female or Male Homozygous for Taglntm2(cre)Yec
Pairs /Price (US dollars $) Pair Genotype $755.30 Heterozygous for Taglntm2(cre)Yec x Homozygous for Taglntm2(cre)Yec Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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Contact information
General inquiriesContracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
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