Strain Name: |
B6.129S6-Taglntm2(cre)Yec/J |
|---|---|
Stock Number: |
006878 |
Availability: | Repository- Live |
General Terms and Conditions |
| Genes & Alleles | Tagln; Taglntm2(cre)Yec; cre; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Mating System Heterozygote x Homozygote (Female x Male) Species laboratory mouse Donating Investigator Yuqing Chen, University of Michigan, Medical School Generation N?F0 (03-DEC-07) Strain Description
Mice homozygous for this SM22alpha-CreKI allele are viable and fertile. These mice have a Cre-recombinase gene inserted into the endogenous transgelin (SM22alpha) locus. The donating investigator reports that this mutation results in a loss of function of the targeted gene. Cre recombinase activity is shown in adult smooth muscle cells (such as arteries, veins, and visceral organs) and cardiac myocytes, but activity is not observed in the same embryonic tissues. These SM22alpha-CreKI mice may be useful for "Cre-lox" technology applications in studying smooth muscle and cardiac gene function, as well as cardiovascular disease.Strain Development
A targeting vector was designed to insert a Cre-recombinase coding sequence (followed by a PGK-neo cassette) in-frame at the SM22alpha (Tagln or transgelin) initiation site. The construct was electroporated into 129S6/SvEvTac-derived "CMT-1" embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Chimeric animals were crossed with C57BL/6 to generate heterozygotes. This strain was backcrossed to C57BL/6J for at least 12 generations prior to arrival at The Jackson Laboratory. The Y chromosome may not have been fixed to the C57BL/6J genetic background.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Taglntm2(cre)Yec | ||
|---|---|---|---|
| Allele Name | targeted mutation 2, Yuqing E Chen | ||
| Common Name(s) | SM22alpha-creKI; Taglntm2(cre)Liw; | ||
| Mutation Made By | Jifeng Zhang, University of Michigan, Medical School | ||
| Strain of Origin | 129S6/SvEvTac | ||
| ES Cell Line Strain | 129 | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Gene Symbol and Name | Tagln, transgelin | ||
| Chromosome | 9 | ||
| Gene Common Name(s) | DKFZp686P11128; SM-22 alpha; SM22; SMCC; Sm22a; TAGLN1; WS3-10; smooth muscle protein, alpha, 22 kDa; | ||
| Molecular Note | The cre recombinase coding sequence was inserted in frame into the initiation site, as well as a PGK-neo. [MGI Ref ID J:119865] | ||
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
TagIntm2(cre)Yec
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, heterozygous females may be bred with homozygous males. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying other alleles of Tagln
008082 B6;SJL-Tg(Tagln-tTA)1Mrab Tg(tetO-Mcpt1)1Mrab/J 006875 FVB/N-Tg(Tagln-rtTA)E1Jwst/J 004746 STOCK Tg(Tagln-cre)1Her/J View Strains carrying other alleles of Tagln (3 strains)
Additional Web Information
Congenic Nomenclature
Cre-lox or FLP-FRT Systems
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
cre relatedCardiovascular Research
Other
Research Tools
Cardiovascular Research (Cre-lox System)
Cre-lox System (Cre-Recombinase Expression: Germline/Embryonic Expression)
Developmental Biology Research (Cre-lox System)
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Genetics Research (Tissue/Cell Markers: Cre-lox System)
Research Tools
Cre-lox System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
References
Selected Reference(s)
Zhang J; Zhong W; Cui T; Yang M; Hu X; Xu K; Xie C; Xue C; Gibbons GH; Liu C; Li L; Chen YE. 2006. Generation of an adult smooth muscle cell-targeted Cre recombinase mouse model. Arterioscler Thromb Vasc Biol 26(3):e23-4. [PubMed: 16484601] [MGI Ref ID J:119865]
Price and Supply Information
| Strain Name: | B6.129S6-Taglntm2(cre)Yec/J |
| Stock Number: | 006878 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Use restrictions apply, see Policy on Licensing and Use Restrictions.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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