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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Noncarrier x Hemizygote (Female x Male) 13-APR-08 Species laboratory mouse Generation N10F4 (24-MAR-11)
Generation DefinitionsDonating Investigator Donald Kohan, University of Utah Description
Mice hemizygous for this AQP2-Cre transgene are viable and fertile. Transgenic cre activity, directed by the mouse aquaporin 2 promoter, is observed in kidney cells (collecting duct) and testes (sperm). When bred with mice containing a loxP-flanked sequence of interest, cre-mediated recombination will result in deletion of the flanked sequence. In such breedings, maternal inheritance of the transgene is recommended for kidney-specific recombinase activity as males express cre in sperm as well as kidney tissues. These AQP2-Cre mice may be used to generate conditional mutations in the renal collecting duct for studying nephrology, physiology, metabolism, or type II diabetes. In addition, cre expression in sperm may be useful in generating conditional mutations in multiple or all tissues in the resulting offspring.Development
The AQP2-Cre transgene contains a 9.5 kb fragment of the mouse aquaporin 2 gene 5'-flanking region (and not an 11 kb fragment as originally reported) placed upstream of a Cre recombinase sequence (with SV40 nuclear localization signal at the amino terminus and an 11-amino acid epitope tag derived from Herpes simplex virus glycoprotein D located at the carboxy terminus), all followed by an SV40 late region polyadenylation signal. Transgenic founder mice were obtained on the (C57BL/6 x SJL)F2 genetic background. Transgenic females were then backcrossed to C57BL/6 males at least 5 generations prior to arrival at The Jackson Laboratory.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Aqp2
005921 C57BL/6J-Aqp2F204V/J 000530 C57BL/6J-Aqp2cph/J View Strains carrying other alleles of Aqp2 (2 strains)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Tg(Aqp2-cre)1Dek/0
involves: C57BL/6 * SJL
- normal phenotype
- no abnormal phenotype detected
- hemizygous mice are viabl and fertile (MGI Ref ID J:104785)
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cardiovascular Research
Cre-lox System
Cre-lox System
Cre Recombinase Expression
Cre Recombinase Expression: Germline/Embryonic Expression
Developmental Biology Research
Cre-lox System
Diabetes and Obesity Research
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Tissue/Cell Markers
Tissue/Cell Markers: kidney specific marker
Internal/Organ Research
Metabolism Research
Reproductive Biology Research
Cre-lox System
male germ cells
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Aqp2-cre)1Dek | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Donald E Kohan | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | AQP2-Cre; | ||
| Mutation Made By | Donald Kohan, University of Utah | ||
| Strain of Origin | (C57BL/6 x SJL)F2 | ||
| Site of Expression | kidney cells (collecting duct) and testes (sperm) | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Aqp2, aquaporin 2, mouse, laboratory | ||
| Driver Note | Aqp2 | ||
| Molecular Note | This transgene contains a 9.5 kb fragment of the mouse aquaporin 2 gene 5'-flanking region placed upstream of a Cre recombinase sequence (with SV40 nuclear localization signal at the amino terminus and an 11-amino acid epitope tag derived from Herpes simplex virus glycoprotein D located at the carboxy terminus). The aquaporin regulatory sequences drive Cre expression in the collecting ducts of the kidney and in sperm. [MGI Ref ID J:104785] | ||
| Gene Symbol and Name | Tg(Aqp2-cre)1Dek, transgene insertion 1, Donald E Kohan | ||
| Chromosome | UN | ||
| Gene Common Name(s) | AQP2-Cre; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(Aqp2-cre)1Dek, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Ge Y; Ahn D; Stricklett PK; Hughes AK; Yanagisawa M; Verbalis JG; Kohan DE. 2005. Collecting duct-specific knockout of endothelin-1 alters vasopressin regulation of urine osmolality. Am J Physiol Renal Physiol 288(5):F912-20. [PubMed: 15632412] [MGI Ref ID J:104785]
Ge Y; Stricklett PK; Hughes AK; Yanagisawa M; Kohan DE. 2005. Collecting duct-specific knockout of the endothelin A receptor alters renal vasopressin responsiveness, but not sodium excretion or blood pressure. Am J Physiol Renal Physiol 289(4):F692-8. [PubMed: 15928212] [MGI Ref ID J:101241]
Zhang H; Zhang A; Kohan DE; Nelson RD; Gonzalez FJ; Yang T. 2005. Collecting duct-specific deletion of peroxisome proliferator-activated receptor gamma blocks thiazolidinedione-induced fluid retention. Proc Natl Acad Sci U S A 102(26):9406-11. [PubMed: 15956187] [MGI Ref ID J:99873]
Additional References
Tg(Aqp2-cre)1Dek relatedFujiu K; Manabe I; Nagai R. 2011. Renal collecting duct epithelial cells regulate inflammation in tubulointerstitial damage in mice. J Clin Invest 121(9):3425-41. [PubMed: 21821915] [MGI Ref ID J:178256]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, hemizygotes may be bred to wildtype sibling or C57BL/6J mice. Alternatively, homozygous mice may be interbred. For "Cre-lox" studies, maternal inheritance of the transgene is recommended as males express cre in sperm as well as kidney tissues. Mating System Noncarrier x Hemizygote (Female x Male) 13-APR-08 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $261.00 Female or Male Hemizygous for Tg(Aqp2-cre)1Dek
Pairs /Price (US dollars $) Pair Genotype $321.00 Hemizygous for Tg(Aqp2-cre)1Dek x Noncarrier $321.00 Noncarrier x Hemizygous for Tg(Aqp2-cre)1Dek Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $339.30 Female or Male Hemizygous for Tg(Aqp2-cre)1Dek
Pairs /Price (US dollars $) Pair Genotype $417.30 Hemizygous for Tg(Aqp2-cre)1Dek x Noncarrier $417.30 Noncarrier x Hemizygous for Tg(Aqp2-cre)1Dek Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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|
|
Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
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