Strain Name: |
B6.Cg-Tg(Aqp2-cre)1Dek/J |
|---|---|
Stock Number: |
006881 |
Availability: | Repository- Live |
Price and Supply Information | |
General Terms and Conditions |
| Genes & Alleles | Aqp2; cre; Tg(Aqp2-cre)1Dek; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Mating System Noncarrier x Hemizygote (Female x Male) Species laboratory mouse Donating Investigator Donald Kohan, University of Utah Generation N6+ (18-DEC-07) Strain Description
Mice hemizygous for this AQP2-Cre transgene are viable and fertile. Transgenic cre activity, directed by the mouse aquaporin 2 promoter, is observed in kidney cells (collecting duct) and testes (sperm). When bred with mice containing a loxP-flanked sequence of interest, cre-mediated recombination will result in deletion of the flanked sequence. In such breedings, maternal inheritance of the transgene is recommended for kidney-specific recombinase activity as males express cre in sperm as well as kidney tissues. These AQP2-Cre mice may be used to generate conditional mutations in the renal collecting duct for studying nephrology, physiology, metabolism, or type II diabetes. In addition, cre expression in sperm may be useful in generating conditional mutations in multiple or all tissues in the resulting offspring.Strain Development
The AQP2-Cre transgene contains a 9.5 kb fragment of the mouse aquaporin 2 gene 5'-flanking region (and not an 11 kb fragment as originally reported) placed upstream of a Cre recombinase sequence (with SV40 nuclear localization signal at the amino terminus and an 11-amino acid epitope tag derived from Herpes simplex virus glycoprotein D located at the carboxy terminus), all followed by an SV40 late region polyadenylation signal. Transgenic founder mice were obtained on the (C57BL/6 x SJL)F2 genetic background. Transgenic females were then backcrossed to C57BL/6 males at least 5 generations prior to arrival at The Jackson Laboratory.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Tg(Aqp2-cre)1Dek | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Donald E Kohan | ||
| Common Name(s) | AQP2-Cre; | ||
| Mutation Made By | Donald Kohan, University of Utah | ||
| Strain of Origin | (C57BL/6 x SJL)F2 | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Aqp2, aquaporin 2, mouse, laboratory | ||
| Molecular Note | This transgene contains a 9.5 kb fragment of the mouse aquaporin 2 gene 5'-flanking region placed upstream of a Cre recombinase sequence (with SV40 nuclear localization signal at the amino terminus and an 11-amino acid epitope tag derived from Herpes simplex virus glycoprotein D located at the carboxy terminus). The aquaporin regulatory sequences drive Cre expression in the collecting ducts of the kidney and in sperm. [MGI Ref ID J:104785] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for JAX® GEMM® Strains | ||
Genotyping Protocols
Tg(Aqp2-cre)1Dek
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, hemizygotes may be bred to wildtype sibling or C57BL/6J mice. Alternatively, homozygous mice may be interbred. For "Cre-lox" studies, maternal inheritance of the transgene is recommended as males express cre in sperm as well as kidney tissues. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying other alleles of Aqp2
005921 C57BL/6J-Aqp2F204V/J 000530 C57BL/6J-Aqp2cph/J View Strains carrying other alleles of Aqp2 (2 strains)
Additional Web Information
Congenic Nomenclature
Cre-lox or FLP-FRT Systems
Animal Health Reports
Room Number AX12
Research Applications
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cardiovascular Research (Cre-lox System)
Cre-lox System (Cre-Recombinase Expression: Germline/Embryonic Expression)
Developmental Biology Research (Cre-lox System)
Diabetes and Obesity Research
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Genetics Research (Tissue/Cell Markers: kidney specific marker)
Internal/Organ Research
Metabolism Research
Reproductive Biology Research (Cre-lox System)
Reproductive Biology Research (male germ cells)
Research Tools
Cre-lox System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
References
Selected Reference(s)
Ge Y; Ahn D; Stricklett PK; Hughes AK; Yanagisawa M; Verbalis JG; Kohan DE. 2005. Collecting duct-specific knockout of endothelin-1 alters vasopressin regulation of urine osmolality. Am J Physiol Renal Physiol 288(5):F912-20. [PubMed: 15632412] [MGI Ref ID J:104785]
Ge Y; Stricklett PK; Hughes AK; Yanagisawa M; Kohan DE. 2005. Collecting duct-specific knockout of the endothelin A receptor alters renal vasopressin responsiveness, but not sodium excretion or blood pressure. Am J Physiol Renal Physiol 289(4):F692-8. [PubMed: 15928212] [MGI Ref ID J:101241]
Zhang H; Zhang A; Kohan DE; Nelson RD; Gonzalez FJ; Yang T. 2005. Collecting duct-specific deletion of peroxisome proliferator-activated receptor gamma blocks thiazolidinedione-induced fluid retention. Proc Natl Acad Sci U S A 102(26):9406-11. [PubMed: 15956187] [MGI Ref ID J:99873]
Price and Supply Information
| Strain Name: | B6.Cg-Tg(Aqp2-cre)1Dek/J |
| Stock Number: | 006881 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. The shipping destinations are:
- USA, Canada and Mexico
- International
*Pricing for Shipping Destination selected:
International
| Price(s) in US dollars ($) | Genotype(s) Provided | ||||
|---|---|---|---|---|---|
| Individual Mouse Price | $307.40 | Hemizygous for Tg(Aqp2-cre)1Dek | |||
| Pair | $375.30 | Hemizygous for Tg(Aqp2-cre)1Dek x Noncarrier | |||
| Pair | $375.30 | Noncarrier x Hemizygous for Tg(Aqp2-cre)1Dek | |||
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
Histology and Tissue Collection Services are available for all JAX® Mice strains. For more information, please contact Customer Service at orderquest@jax.org or 1-207-288-5845. Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. View Control Pricing Information for JAX® Strains. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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Contact Information
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Tel: 800.422.6423 or 207.288.5845
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