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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Former Names STOCK Tg(ACTB-Bgeo,-AML1/ETO,-ALPP)1Lbe/J (Changed: 08-MAY-08 ) STOCK Tg(ACTB-Bgeo,AML1/ETO,ALPP)1Lbe/J (Changed: 19-APR-07 ) Type Mutant Stock; Transgenic; Additional information on Genetically Engineered Mutant Mice. Mating System +/+ sibling x Hemizygote (Female x Male) Species laboratory mouse Generation N2+F3 (20-MAY-08) Donating Investigator Corrinne Lobe, Sunnybrook & Women's College HSC Description
Mice hemizygous for this "Z/AP-AML1-ETO" transgene (coding for the translocation t(8;21) present in 15% of acute myeloid leukemias (AML)) are viable and fertile. Homozygotes die in utero presumably due to high lacZ expression. Prior to cre-mediated excision of the "floxed" STOP sequence, expression of lacZ is observed in all tissues including bone marrow progenitor cells. When bred to Cre recombinase expressing mice, the STOP sequence (and beta-geo) is removed in the resulting offspring, allowing transcription/co-expression of both the human AML1-ETO fusion protein and placental alkaline phosphatase (ALPP or PLAP) to proceed in the Cre recombinase expressing cells. While pan expression of AML1-ETO leads to embryonic lethality (E7.5), hematopoietic and endothelial expression leads to malignancy in B- and T- lymphoid cells and secondary mutations that closely resemble the association of AML1-ETO with acute myeloid leukemia in humans. These transgenic mice may be useful for global expression of lacZ or, when crossed to a Cre-recombinase expressing strain, for studying hematopoietic development, cancer, and leukemia.Development
A transgenic construct was designed with the CMV enhancer/chicken beta-actin promoter, loxP-flanked STOP sequence (beta-geo reporter followed by SV40 polyadenylation signal), human AML1-ETO fusion protein sequence (coding for the translocation t(8;21) present in 15% of acute myeloid leukemias (AML)), internal ribosomal entry site, and the human Placental Alkaline Phosphatase (ALPP or hPLAP) gene. This construct was electroporated into 129X1/SvJ x 129S1/Sv-derived R1 embryonic stem (ES) cells. ES cells with strong lacZ expression, single copy transgene integration, and efficient expression of EGFP following Cre-mediated excision of the STOP sequence were selected and aggregated with 2.5 dpc 8-cell CD1 mouse embryos. Following transfer into pseudo-pregnant foster mothers, chimeric males were mated to wildtype CD1 females to establish founder line mice. This strain was maintained by breeding hemizygous mice to CD1 or wildtype siblings for an unknown number of generations prior to arrival at The Jackson Laboratory. Upon arrival, these mice were bred at least one generation to C57BL/6J.
Control Information
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| Noncarrier | ||
| Considerations for Choosing Controls | ||
Related Strains
lacZ Expression Strains
View lacZ Expression Strains (175 strains)
Additional Web Information
Cre-lox Systems
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Bgeo relatedCancer Research
Increased Tumor Incidence (Leukemia)
Developmental Biology Research
Embryonic Lethality (Homozygous)
Lymphoid Tissue Defects (hematopoietic defects)
Hematological Research
Hematopoietic Defects
Research Tools
lacZ Expression
Cancer Research (Leukemia)
Cre-lox System (loxP-flanked Sequences)
Genetics Research (Tissue/Cell Markers: Cre-lox System)
Hematological Research
Research Tools
lacZ Expression
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(CAG-Bgeo,-AML1/ETO,-ALPP)1Lbe | ||
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| Allele Name | transgene insertion 1, Corinne G Lobe | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | Tg(ACTB-Bgeo,-AML1/ETO,-ALPP)1Lbe; Tg(CAG-Bgeo,-AML1/ETO,-ALPP)1Lbe; ZAP-AML1-ETO; | ||
| Mutation Made By | Corrinne Lobe, Sunnybrook & Women's College HSC | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| ES Cell Line Name | R1 | ||
| ES Cell Line Strain | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Site of Expression | lacZ is highly expressed in most cells and tissues, including bone marrow progenitor cells. When bred to Cre recombinase expressing mice, lacZ expression is replaced with co-expression of the human AML1-ETO fusion protein and placental alkaline phosphatase (ALPP or PLAP) in Cre recombinase expressing cells. | ||
| Expressed Gene | ALPP, alkaline phosphatase, placental (Regan isozyme), human | ||
| Expressed Gene | Bgeo, fusion of beta-galactosidase and neomycin phosphotransferase genes , E. coli | ||
| Promoter | ACTB, actin, beta, chicken | ||
| Molecular Note | A transgenic construct was designed with the CMV enhancer/chicken beta-actin promoter, loxP-flanked STOP sequence (beta-geo reporter followed by SV40 polyadenylation signal), human AML1-ETO fusion protein sequence (coding for the translocation t(8;21) present in 15% of acute myeloid leukemias (AML)), internal ribosomal entry site, and the human Placental Alkaline Phosphatase (ALPP or hPLAP) gene. Expression of lacZ is observed in all tissues including bone marrow progenitor cells. When bred to Cre recombinase expressing mice, the STOP sequence (and beta-geo) is removed in the resulting offspring, allowing transcription/co-expression of both the human AML1-ETO fusion protein and placental alkaline phosphatase (ALPP or PLAP) to proceed in the Cre-expressing cells. [MGI Ref ID J:119131] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(CAG-Bgeo,-AML1/ETO,-ALPP)1Lbe, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Additional References
Lobe CG; Koop KE; Kreppner W; Lomeli H; Gertsenstein M; Nagy A. 1999. Z/AP, a double reporter for cre-mediated recombination. Dev Biol 208(2):281-92. [PubMed: 10191045] [MGI Ref ID J:93700]
Tg(CAG-Bgeo,-AML1/ETO,-ALPP)1Lbe relatedGuo C; Lobe CG. 2007. Cre-conditional expression of the AML1-ETO fusion in transgenic mice MGI Direct Data Submission :. [MGI Ref ID J:119131]
Lobe CG; Koop KE; Kreppner W; Lomeli H; Gertsenstein M; Nagy A. 1999. Z/AP, a double reporter for cre-mediated recombination. Dev Biol 208(2):281-92. [PubMed: 10191045] [MGI Ref ID J:93700]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, hemizygotes are bred to wildtype siblings. Homozygous mice die in utero presumably due to high lacZ expression. Mating System +/+ sibling x Hemizygote (Female x Male) Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $236.40 Female or Male Hemizygous for Tg(CAG-Bgeo,-AML1/ETO,-ALPP)1Lbe *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $288.65 Hemizygous for Tg(CAG-Bgeo,-AML1/ETO,-ALPP)1Lbe x Noncarrier $288.65 Noncarrier x Hemizygous for Tg(CAG-Bgeo,-AML1/ETO,-ALPP)1Lbe
Additional Supply Details
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| Pricing for International shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $307.40 Female or Male Hemizygous for Tg(CAG-Bgeo,-AML1/ETO,-ALPP)1Lbe *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $375.30 Hemizygous for Tg(CAG-Bgeo,-AML1/ETO,-ALPP)1Lbe x Noncarrier $375.30 Noncarrier x Hemizygous for Tg(CAG-Bgeo,-AML1/ETO,-ALPP)1Lbe
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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Control Information
| Control | ||
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| Noncarrier | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
See Terms of Use
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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Terms of Use
Terms of Use
General Terms and Conditions
Use restrictions apply, see Policy on Licensing and Use Restrictions.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
Contact information
General inquiries
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| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
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