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- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names STOCK Tg(ACTB-Bgeo,-AML1/ETO,-ALPP)1Lbe/J (Changed: 08-MAY-08 ) STOCK Tg(ACTB-Bgeo,AML1/ETO,ALPP)1Lbe/J (Changed: 19-APR-07 ) Type Mutant Stock; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation N2+F2pN1
Generation DefinitionsDonating Investigator Corrinne Lobe, Sunnybrook & Women's College HSC Description
Mice hemizygous for this "Z/AP-AML1-ETO" transgene (coding for the translocation t(8;21) present in 15% of acute myeloid leukemias (AML)) are viable and fertile. Homozygotes die in utero presumably due to high lacZ expression. Prior to cre-mediated excision of the "floxed" STOP sequence, expression of lacZ is observed in all tissues including bone marrow progenitor cells. When bred to Cre recombinase expressing mice, the STOP sequence (and beta-geo) is removed in the resulting offspring, allowing transcription/co-expression of both the human AML1-ETO fusion protein and placental alkaline phosphatase (ALPP or PLAP) to proceed in the Cre recombinase expressing cells. While pan expression of AML1-ETO leads to embryonic lethality (E7.5), hematopoietic and endothelial expression leads to malignancy in B- and T- lymphoid cells and secondary mutations that closely resemble the association of AML1-ETO with acute myeloid leukemia in humans. These transgenic mice may be useful for global expression of lacZ or, when crossed to a Cre-recombinase expressing strain, for studying hematopoietic development, cancer, and leukemia.Development
A transgenic construct was designed with the CMV enhancer/chicken beta-actin promoter, loxP-flanked STOP sequence (beta-geo reporter followed by SV40 polyadenylation signal), human AML1-ETO fusion protein sequence (coding for the translocation t(8;21) present in 15% of acute myeloid leukemias (AML)), internal ribosomal entry site, and the human Placental Alkaline Phosphatase (ALPP or hPLAP) gene. This construct was electroporated into 129X1/SvJ x 129S1/Sv-derived R1 embryonic stem (ES) cells. ES cells with strong lacZ expression, single copy transgene integration, and efficient expression of EGFP following Cre-mediated excision of the STOP sequence were selected and aggregated with 2.5 dpc 8-cell CD1 mouse embryos. Following transfer into pseudo-pregnant foster mothers, chimeric males were mated to wild-type CD1 females to establish founder line mice. This strain was maintained by breeding hemizygous mice to CD1 or wild-type siblings for an unknown number of generations prior to arrival at The Jackson Laboratory. Upon arrival, these mice were bred at least one generation to C57BL/6J.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
Related Strains
lacZ Expression Strains
View lacZ Expression Strains (255 strains)
012377 B6.129S-Cyp19a1tm1.1Shah/J 012588 B6.Cg-Tg(TH-ALPP)1Erav/J 008226 B6.FVB-Tg(CAG-EGFP,-ALPP)2.6Ggc/J 007066 B6.SJL-Tg(Crx-GFP,-ALPP)1Clc/J 017524 B6;129-Tg(CMV-Bgeo,-WGA,-ALPP)1Mgmj/J 016555 B6;SJL-Tg(Nqo1-ALPP)1Jaj/J 008200 FVB/N-Tg(CAG-EGFP,-ALPP)2.6Ggc/J 006613 STOCK Tg(CAG-Bgeo,-Tle1,-ALPP)1Lbe/J 003919 STOCK Tg(CAG-Bgeo/ALPP)1Lbe/J 005105 STOCK Tg(Chx10-EGFP/cre,-ALPP)2Clc/J 014600 STOCK Tg(I12b-cre/ERT2,-ALPP)37Fsh/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Bgeo relatedCancer Research
Increased Tumor Incidence
Leukemia
Developmental Biology Research
Embryonic Lethality (Homozygous)
Lymphoid Tissue Defects
hematopoietic defects
Hematological Research
Hematopoietic Defects
Research Tools
lacZ Expression
Cancer Research
Leukemia
Cre-lox System
loxP-flanked Sequences
Genetics Research
Tissue/Cell Markers
Tissue/Cell Markers: Cre-lox System
Hematological Research
Research Tools
lacZ Expression
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(CAG-Bgeo,-AML1/ETO,-ALPP)1Lbe | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Corinne G Lobe | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | Tg(ACTB-Bgeo,-AML1/ETO,-ALPP)1Lbe; Tg(CAG-Bgeo,-AML1/ETO,-ALPP)1Lbe; ZAP-AML1-ETO; | ||
| Mutation Made By | Corrinne Lobe, Sunnybrook & Women's College HSC | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| ES Cell Line Name | R1 | ||
| ES Cell Line Strain | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Site of Expression | lacZ is highly expressed in most cells and tissues, including bone marrow progenitor cells. When bred to Cre recombinase expressing mice, lacZ expression is replaced with co-expression of the human AML1-ETO fusion protein and placental alkaline phosphatase (ALPP or PLAP) in Cre recombinase expressing cells. | ||
| Expressed Gene | ALPP, alkaline phosphatase, placental, human | ||
| Expressed Gene | Bgeo, fusion of beta-galactosidase and neomycin phosphotransferase genes, E. coli | ||
| Promoter | ACTB, actin, beta, chicken | ||
| Molecular Note | A transgenic construct was designed with the CMV enhancer/chicken beta-actin promoter, loxP-flanked STOP sequence (beta-geo reporter followed by SV40 polyadenylation signal), human AML1-ETO fusion protein sequence (coding for the translocation t(8;21) present in 15% of acute myeloid leukemias (AML)), internal ribosomal entry site, and the human Placental Alkaline Phosphatase (ALPP or hPLAP) gene. Expression of lacZ is observed in all tissues including bone marrow progenitor cells. When bred to Cre recombinase expressing mice, the STOP sequence (and beta-geo) is removed in the resulting offspring, allowing transcription/co-expression of both the human AML1-ETO fusion protein and placental alkaline phosphatase (ALPP or PLAP) to proceed in the Cre-expressing cells. [MGI Ref ID J:119131] | ||
| Gene Symbol and Name | Tg(CAG-Bgeo,-AML1/ETO,-ALPP)1Lbe, transgene insertion 1, Corinne G Lobe | ||
| Chromosome | UN | ||
| Gene Common Name(s) | Tg(ACTB-Bgeo,-AML1/ETO,-ALPP)1Lbe; ZAP-AML1-ETO; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(CAG-Bgeo,-AML1/ETO,-ALPP)1Lbe, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Additional References
Lobe CG; Koop KE; Kreppner W; Lomeli H; Gertsenstein M; Nagy A. 1999. Z/AP, a double reporter for cre-mediated recombination. Dev Biol 208(2):281-92. [PubMed: 10191045] [MGI Ref ID J:93700]
Tg(CAG-Bgeo,-AML1/ETO,-ALPP)1Lbe relatedGuo C; Lobe CG. 2007. Cre-conditional expression of the AML1-ETO fusion in transgenic mice MGI Direct Data Submission :. [MGI Ref ID J:119131]
Lobe CG; Koop KE; Kreppner W; Lomeli H; Gertsenstein M; Nagy A. 1999. Z/AP, a double reporter for cre-mediated recombination. Dev Biol 208(2):281-92. [PubMed: 10191045] [MGI Ref ID J:93700]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry When maintaining a live colony, hemizygotes are bred to wildtype siblings. Homozygous mice die in utero presumably due to high lacZ expression.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
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