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Strain Name:

B6.Cg-Ldlrtm1Her Sod2tm1Leb/J

Stock Number:

006883

Availability:

Repository- Live

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General Terms and Conditions

Strain Common Names      LDLR MnSOD;
Genes & Alleles   Ldlr;   Ldlrtm1Her;   Sod2;   Sod2tm1Leb;

Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Mutant Strain
Type JAX® GEMM® Strain - Targeted Mutation
Mating SystemSee Colony Maintenance         (Female x Male)
Specieslaboratory mouse
Donating Investigator Jan Breslow,   Rockefeller University
GenerationN7+N1F1 (20-DEC-07)

Strain Description
Independently, mice that are homozygous for this MnSOD mutation (Sod2tm1Leb) allele exhibit postnatal lethality and exhibit anemia, degeneration of neurons in the basal ganglia and brainstem, progressive motor disturbances, and myocardial injury. Individual LDLR homozygous mutants are predisposed to atherosclerosis. When mutant mice are homozygous for both alleles, they die in utero. Mice heterozygous for the Sod2 mutation and homozygous for the LDLR are viable and fertile. The mice may be useful in studies of diabetes, metabolism, hyperglycemia, atherosclerosis, and hypercholesterolemia, and oxidative stress.

Strain Development
The Ldlrtm1Her mutation was made by Dr. Robert Hammer and Joachim Herz (HHMI, University of Texas Southwestern Medical Center). Briefly, a targeting vector was used to insert a neo cassette into exon 4. The vector was electroporated into 129S7/SvEvBrd-derived AB1 embryonic stem (ES) cells. Chimeric mice were bred to C57BL/6J, and the strain was made congenic on a C57BL/6J genetic background at The Jackson Laboratory (Stock No. 002207). The Sod2{super tm1Leb} was created by Dr. Russell Lebovitz (Baylor College of Medicine) by replacing exons 1-2 with a PGK-HPRT minigene. The targeting vector was electroporated into 129S7/SvEvBrd-derived AB2.1 and then maintained on a congenic genetic background at The Jackson Laboratory (Stock No. 002973). To generate the double mutant strain, Stock No. 002207 mice were bred with Stock No. 002973 mice in the laboratory of Dr. Jan Breslow at The Rockefeller University. Double mutant mice were then backcrossed to C57BL/6J for approximately 7 generations prior to arrival at The Jackson Laboratory. The donating investigators indicate that 103/104 microsatellite markers indicate C57BL/6J background. The single exception is D17Mit143.2.

Gene & Allele Details

Allele Symbol Ldlrtm1Her
Allele Name targeted mutation 1, Joachim Herz
Common Name(s) LDLR KO; LDLR-; LDLr0; LDLrKO; Ldlrtm1Her;
Mutation Made By Joachim Herz,   Univ of Texas Southwest Med Ctr Dallas
Strain of Origin129S7/SvEvBrd-Hprt1<+>
ES Cell Line NameAB1
ES Cell Line Strain129S7/SvEvBrd-Hprt1<+>
Gene Symbol and Name Ldlr, low density lipoprotein receptor
Chromosome 9
Gene Common Name(s) FH; FHC; LDLRA;
General Note When used in bone marrow transplant into Ldlrtm1Her homozygous mice, Abca1tm1Jdm Abcg1tm1Dgen homozygous cells accelerate the development of atherosclerosis. (J:130777)
Molecular Note Insertion of a neomycin resistance cassette into exon 4. The authors predict that the targeted allele would encode a truncated non-functional protein that will not bind LDL, and that lacks a membrane spanning segment. Immunoblot analysis of liver membranes detected a truncated protein in homozygous mutant animals. [MGI Ref ID J:37394]
 
Allele Symbol Sod2tm1Leb
Allele Name targeted mutation 1, Russell M Lebovitz
Common Name(s) SOD2m1BCM; Sod2-; Sod2mlbcm;
Mutation Made By Russell Lebovitz,   SUMA Partners
Strain of Origin129S7/SvEvBrd-Hprt1
ES Cell Line NameAB2.1
ES Cell Line Strain129S7/SvEvBrd-Hprt1
Gene Symbol and Name Sod2, superoxide dismutase 2, mitochondrial
Chromosome 17
Gene Common Name(s) IPO-B; MGC:6144; MNSOD; Mn-SOD; Sod-2; manganese SOD; manganese superoxide dismutase;
Molecular Note A human HPRT minigene driven by the PGK promoter replaced exons 1 and 2, and sequences approximately 500 bp immediately 5' of exon 1. The replaced region encodes the transcription and translation start sites, the mitochondrial targeting sequence, and oneof three histidines that bind directly to the manganese cofactor. Northern blot analysis of brain did not detect mRNA in homozygous mutant mice. Enzyme activity assays of heart did not detect active protein in homozygous mutant mice. [MGI Ref ID J:36148]

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Genotyping Protocols

Human HPRT
Ldlr tm1Her

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice are bred as heterozygous for the Sod2 mutation and homozygous for the Ldlr mutation.
Diet Information LabDiet® 5K52/5K67

Related Strains

View Strains carrying   Ldlrtm1Her     (11 strains)

Strains carrying   Sod2tm1Leb allele
002973   B6.129S7-Sod2tm1Leb/J
View Strains carrying   Sod2tm1Leb     (1 strain)

Strains carrying other alleles of Ldlr
005061   C57BL/6J-LdlrHlb301/J
View Strains carrying other alleles of Ldlr     (1 strain)

Additional Web Information

Congenic Nomenclature

Animal Health Reports

Room Number           AX11

Research Applications

This mouse can be used to support research in many areas including:

Cardiovascular Research
Atherosclerosis
Hypercholesterolemia
Other (altered fat metabolism)

Developmental Biology Research
Embryonic Lethality (Homozygous)

Diabetes and Obesity Research
Hyperglycemia

Metabolism Research
Lipid Metabolism

Neurobiology Research
Metabolic Defects

Research Tools
Cardiovascular Research
Diabetes and Obesity Research
Metabolism Research

Ldlrtm1Her related

Cardiovascular Research
Atherosclerosis
Hypercholesterolemia

Metabolism Research
Lipid Metabolism

Mouse/Human Gene Homologs
hypercholesterolemia, familial

Sod2tm1Leb related

Metabolism Research

Neurobiology Research
Metabolic Defects
Neurodegeneration

References

Additional References

Price and Supply Information

Strain Name: B6.Cg-Ldlrtm1Her Sod2tm1Leb/J
Stock Number: 006883

Price Details

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Supply Details

Standard SupplyRepository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes Usually shipped between four and eight weeks of age.
This strain is included in the Induced Mutant Resource Colony collection.
LicensingSee General Terms and Conditions below  
Control InformationView Control Information in Strain Details.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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