Strain Name: |
B6.Cg-Ldlrtm1Her Sod2tm1Leb/J |
|---|---|
Stock Number: |
006883 |
Availability: | Repository- Live |
Price and Supply Information | |
General Terms and Conditions |
| Strain Common Names | LDLR MnSOD; |
| Genes & Alleles | Ldlr; Ldlrtm1Her; Sod2; Sod2tm1Leb; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Mating System See Colony Maintenance (Female x Male) Species laboratory mouse Donating Investigator Jan Breslow, Rockefeller University Generation N7+N1F1 (20-DEC-07) Strain Description
Independently, mice that are homozygous for this MnSOD mutation (Sod2tm1Leb) allele exhibit postnatal lethality and exhibit anemia, degeneration of neurons in the basal ganglia and brainstem, progressive motor disturbances, and myocardial injury. Individual LDLR homozygous mutants are predisposed to atherosclerosis. When mutant mice are homozygous for both alleles, they die in utero. Mice heterozygous for the Sod2 mutation and homozygous for the LDLR are viable and fertile. The mice may be useful in studies of diabetes, metabolism, hyperglycemia, atherosclerosis, and hypercholesterolemia, and oxidative stress.Strain Development
The Ldlrtm1Her mutation was made by Dr. Robert Hammer and Joachim Herz (HHMI, University of Texas Southwestern Medical Center). Briefly, a targeting vector was used to insert a neo cassette into exon 4. The vector was electroporated into 129S7/SvEvBrd-derived AB1 embryonic stem (ES) cells. Chimeric mice were bred to C57BL/6J, and the strain was made congenic on a C57BL/6J genetic background at The Jackson Laboratory (Stock No. 002207). The Sod2{super tm1Leb} was created by Dr. Russell Lebovitz (Baylor College of Medicine) by replacing exons 1-2 with a PGK-HPRT minigene. The targeting vector was electroporated into 129S7/SvEvBrd-derived AB2.1 and then maintained on a congenic genetic background at The Jackson Laboratory (Stock No. 002973). To generate the double mutant strain, Stock No. 002207 mice were bred with Stock No. 002973 mice in the laboratory of Dr. Jan Breslow at The Rockefeller University. Double mutant mice were then backcrossed to C57BL/6J for approximately 7 generations prior to arrival at The Jackson Laboratory. The donating investigators indicate that 103/104 microsatellite markers indicate C57BL/6J background. The single exception is D17Mit143.2.
Gene & Allele Details
| Allele Symbol | Ldlrtm1Her | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Joachim Herz | ||
| Common Name(s) | LDLR KO; LDLR-; LDLr0; LDLrKO; Ldlrtm1Her; | ||
| Mutation Made By | Joachim Herz, Univ of Texas Southwest Med Ctr Dallas | ||
| Strain of Origin | 129S7/SvEvBrd-Hprt1<+> | ||
| ES Cell Line Name | AB1 | ||
| ES Cell Line Strain | 129S7/SvEvBrd-Hprt1<+> | ||
| Gene Symbol and Name | Ldlr, low density lipoprotein receptor | ||
| Chromosome | 9 | ||
| Gene Common Name(s) | FH; FHC; | ||
| General Note | When used in bone marrow transplant into Ldlrtm1Her homozygous mice, Abca1tm1Jdm Abcg1tm1Dgen homozygous cells accelerate the development of atherosclerosis. (J:130777) | ||
| Molecular Note | Insertion of a neomycin resistance cassette into exon 4. The authors predict that the targeted allele would encode a truncated non-functional protein that will not bind LDL, and that lacks a membrane spanning segment. Immunoblot analysis of liver membranes detected a truncated protein in homozygous mutant animals. [MGI Ref ID J:37394] | ||
| Allele Symbol | Sod2tm1Leb | ||
| Allele Name | targeted mutation 1, Russell M Lebovitz | ||
| Common Name(s) | SOD2m1BCM; Sod2-; Sod2mlbcm; | ||
| Mutation Made By | Russell Lebovitz, SUMA Partners | ||
| Strain of Origin | 129S7/SvEvBrd-Hprt1 | ||
| ES Cell Line Name | AB2.1 | ||
| ES Cell Line Strain | 129S7/SvEvBrd-Hprt1 | ||
| Gene Symbol and Name | Sod2, superoxide dismutase 2, mitochondrial | ||
| Chromosome | 17 | ||
| Gene Common Name(s) | IPO-B; MGC:6144; MNSOD; Mn-SOD; Sod-2; manganese SOD; manganese superoxide dismutase; | ||
| Molecular Note | A human HPRT minigene driven by the PGK promoter replaced exons 1 and 2, and sequences approximately 500 bp immediately 5' of exon 1. The replaced region encodes the transcription and translation start sites, the mitochondrial targeting sequence, and oneof three histidines that bind directly to the manganese cofactor. Northern blot analysis of brain did not detect mRNA in homozygous mutant mice. Enzyme activity assays of heart did not detect active protein in homozygous mutant mice. [MGI Ref ID J:36148] | ||
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for JAX® GEMM® Strains | ||
Genotyping Protocols
Human HPRT
Ldlr tm1Her
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, these mice are bred as heterozygous for the Sod2 mutation and homozygous for the Ldlr mutation. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying Ldlrtm1Her allele
002246 B6.129-Apoetm1Unc Ldlrtm1Her/J 002207 B6.129S7-Ldlrtm1Her/J 006952 B6.Cg-Akt2tm1.1Mbb Ldlrtm1Her/J 006580 B6.Cg-Ins2Akita Ldlrtm1Her/J 006877 B6.Cg-Ldlrtm1Her Tg(H2-K-AKR1B1)1Tj/J 006906 B6.Cg-Lepob Ldlrtm1Her/J 002245 B6;129-Apoetm1Unc Ldlrtm1Her/J 003000 B6;129S-Apobtm2Sgy Ldlrtm1Her/J 002465 B6;129S-Ldlrtm1Her Lrpap1tm1Her/J 002077 B6;129S7-Ldlrtm1Her/J 004192 STOCK Mttptm2Sgy Ldlrtm1Her Apobtm2Sgy Tg(Mx1-cre)1Cgn/J View Strains carrying Ldlrtm1Her (11 strains)
002973 B6.129S7-Sod2tm1Leb/J
005061 C57BL/6J-LdlrHlb301/J
Additional Web Information
Congenic Nomenclature
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
Ldlrtm1Her relatedCardiovascular Research
Atherosclerosis
Hypercholesterolemia
Other (altered fat metabolism)
Developmental Biology Research
Embryonic Lethality (Homozygous)
Diabetes and Obesity Research
Hyperglycemia
Metabolism Research
Lipid Metabolism
Neurobiology Research
Metabolic Defects
Research Tools
Cardiovascular Research
Diabetes and Obesity Research
Metabolism Research
Sod2tm1Leb relatedCardiovascular Research
Atherosclerosis
Hypercholesterolemia
Metabolism Research
Lipid Metabolism
Mouse/Human Gene Homologs
hypercholesterolemia, familial
Metabolism Research
Neurobiology Research
Metabolic Defects
Neurodegeneration
References
Additional ReferencesPrice and Supply Information
| Strain Name: | B6.Cg-Ldlrtm1Her Sod2tm1Leb/J |
| Stock Number: | 006883 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. The shipping destinations are:
- USA, Canada and Mexico
- International
*Pricing for Shipping Destination selected:
International
| Price(s) in US dollars ($) | Genotype(s) Provided | ||||
|---|---|---|---|---|---|
| Individual Mouse Price | $307.40 | Homozygous for Ldlrtm1Her, Heterozygous for Sod2tm1Leb | |||
| Individual Mouse Price | $307.40 | Homozygous for Ldlrtm1Her, Wild-type for Sod2tm1Leb | |||
| Pair | $614.70 | Homozygous for Ldlrtm1Her, Heterozygous for Sod2tm1Leb x Homozygous for Ldlrtm1Her, Wild-type for Sod2tm1Leb | |||
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Histology and Tissue Collection Services are available for all JAX® Mice strains. For more information, please contact Customer Service at orderquest@jax.org or 1-207-288-5845. Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below |
| Control Information | View Control Information in Strain Details. View Control Pricing Information for JAX® Strains. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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