Strain Name: |
C57BL/6-Tg(Zp3-cre)1Gwh/J |
|---|---|
Stock Number: |
006888 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Genes & Alleles | Zp3; cre; Tg(Zp3-cre)1Gwh; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Transgenic Additional information on JAX® GEMM® Strains. Species laboratory mouse Donating Investigator Jamey Marth, University of California at San Diego Generation N?pN1 Strain Description
This is a transgenic line in which Cre recombinase expression is controlled by the regulatory sequences from the mouse zona pellucida 3 (Zp3) gene, which is normally expressed exclusively in the growing oocyte prior to the completion of the first meiotic division. This strain is useful for deleting a loxP-flanked genomic segment during oogenesis and thereby producing embryos and offspring that bear the recombined and deleted genomic element(s).Strain Development
The ZP3-Cre transgene was constructed by linking the Zp3 promoter to the Cre gene as well as the human growth hormone splicing and polyadenylation signals.
Gene & Allele Details
| Allele Symbol | Tg(Zp3-cre)1Gwh | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Gerald W Hart | ||
| Common Name(s) | ZP3-Cre; ZP3Cre; | ||
| Mutation Made By | Jamey Marth, University of California at San Diego | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Zp3, zona pellucida glycoprotein 3, mouse, laboratory | ||
| Molecular Note | A 6 kb fragment encompassing the promoter sequence of Zp3 was adjoined to the cre recombinase coding region and human growth hormone splicing and polyadenylation signals. The Zp3 promoter region was reported to direct expression in oocytes. [J:102175] [J:62279] | ||
Genotyping Protocols
Tg(Zp3-cre)1Gwh
Related Strains
Strains carrying other alleles of Zp3
004038 B6.129S4-Zp3tm1Dean/J 003394 C57BL/6-Tg(Zp3-cre)3Mrt/J 003651 C57BL/6-Tg(Zp3-cre)93Knw/J 003377 FVB/N-Tg(Zp3-cre)3Mrt/J 006129 STOCK Tg(Zp3-EGFP)1Dean/J View Strains carrying other alleles of Zp3 (5 strains)
Additional Web Information
Cre-lox or FLP-FRT Systems
Research Applications
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox-System (Cre-Recombinase Expression: Germline/Embryonic Expression)
Research Tools
Cre-lox-System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox system)
References
Selected Reference(s)
Additional ReferencesShafi R; Iyer SP; Ellies LG; O'Donnell N; Marek KW; Chui D; Hart GW; Marth JD. 2000. The O-GlcNAc transferase gene resides on the X chromosome and is essential for embryonic stem cell viability and mouse ontogeny. Proc Natl Acad Sci U S A 97(11):5735-9. [PubMed: 10801981] [J:62279]
Price and Supply Information
| Strain Name: | C57BL/6-Tg(Zp3-cre)1Gwh/J |
| Stock Number: | 006888 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to the Supply Notes for further information. |
|---|---|
| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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