| 
| ||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names B6(CBA)-Tg(Lck-cre)I540Jxm/J (Changed: 02-APR-07 ) Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation ?pN1
Generation DefinitionsDonating Investigator Jamey D Marth, Burnham Inst at Univ Calif Santa Barbara Description
Homozygous mice are viable and have no major defects. This transgenic strain expresses Cre recombinase in thymocytes at lower abundance than line 548-O (Stock No. 003802), resulting in sub-stoichiometric recombination and yielding chimeric cell populations in vivo. In contrast, Lck-Cre transgenic line 548-O recombines loxP-flanked alleles specifically in thymocytes at very high efficiency, up to 99+% as compared with other Lck-Cre transgenic lines.Development
The Cre coding sequence derived from pMC-Cre was cloned into p1017. The transgene was isolated from the plasmid and microinjected into the male pronuclei of B6CBAF2 hybrid zygotes prior to implantation into CD1 foster mothers. A founder expressing the transgene was backcrossed to C57BL/6 by the donating laboratory 9-12 times.
Related Strains
Strains carrying other alleles of Lck
019418 B6(Cg)-Tg(Lck-Cd1d1)1Aben/J 002817 B6.129S2-Lcktm1Mak/J 016101 B6.Cg-Il7rtm1Imx Tg(Lck-Il7r)1676Trma/J 013738 B6.Cg-Tg(LCKprBCL2L1)12Sjk/J 012837 B6.Cg-Tg(Lck-cre)3779Nik/J 003802 B6.Cg-Tg(Lck-cre)548Jxm/J 003202 B6;SJL-Tg(CD8aCD8b)57Scr/J 002427 C3H/He-Tg(LCKprBCL2)36Sjk/J 008842 C57BL/6-Tg(Lck-VIPR2)1Ejg/J 003332 C57BL/6-Tg(LckNotch1)9Erob/J 002230 C57BL/6J-Tg(LckIl4)1315Dbl/J 005732 NOD.Cg-Tg(Lck-cre)548Jxm/AchJ View Strains carrying other alleles of Lck (12 strains)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox System
Cre Recombinase Expression
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Lck-cre)I540Jxm | ||
|---|---|---|---|
| Allele Name | transgene insertion I540, Jamey Marth | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Strain of Origin | (C57BL/6 x CBA)F2 | ||
| Site of Expression | Cre recombinase activity is restricted to thymocytes; evidence of activity in spleen is attributed to the presence of thymus-derived T lymphocytes. | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Lck, lymphocyte protein tyrosine kinase, mouse, laboratory | ||
| Driver Note | Lck | ||
| Molecular Note | The Cre recombinase coding sequence, modified by addition of a Kozak consensus translation start sequence and nuclear localization signals, was inserted into the p1017 expression vector between the thymocyte-specific, mouse proximal Lck promoter and mostof the human growth hormone gene, extending from within exon 1 through the polyadenylation signal. Cre recombinase activity is restricted to thymocytes; evidence of activity in spleen is attributed to the presence of thymus-derived T lymphocytes. [MGI Ref ID J:67674] [MGI Ref ID J:81268] | ||
| Gene Symbol and Name | Tg(Lck-cre)I540Jxm, transgene insertion I540, Jamey Marth | ||
| Chromosome | UN | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(Lck-cre)I540Jxm, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Hennet T; Hagen FK; Tabak LA; Marth JD. 1995. T-cell-specific deletion of a polypeptide N-acetylgalactosaminyl-transferase gene by site-directed recombination. Proc Natl Acad Sci U S A 92(26):12070-4. [PubMed: 8618846] [MGI Ref ID J:67674]
Additional References
Tg(Lck-cre)I540Jxm relatedChaffin KE; Beals CR; Wilkie TM; Forbush KA; Simon MI; Perlmutter RM. 1990. Dissection of thymocyte signaling pathways by in vivo expression of pertussis toxin ADP-ribosyltransferase. EMBO J 9(12):3821-9. [PubMed: 2123451] [MGI Ref ID J:81268]
Chang HC; Han L; Jabeen R; Carotta S; Nutt SL; Kaplan MH. 2009. PU.1 regulates TCR expression by modulating GATA-3 activity. J Immunol 183(8):4887-94. [PubMed: 19801513] [MGI Ref ID J:153816]
Chang HC; Sehra S; Goswami R; Yao W; Yu Q; Stritesky GL; Jabeen R; McKinley C; Ahyi AN; Han L; Nguyen ET; Robertson MJ; Perumal NB; Tepper RS; Nutt SL; Kaplan MH. 2010. The transcription factor PU.1 is required for the development of IL-9-producing T cells and allergic inflammation. Nat Immunol 11(6):527-34. [PubMed: 20431622] [MGI Ref ID J:160612]
Jabeen R; Chang HC; Goswami R; Nutt SL; Kaplan MH. 2011. The transcription factor PU.1 regulates gammadelta T cell homeostasis. PLoS One 6(7):e22189. [PubMed: 21779390] [MGI Ref ID J:175789]
Jin W; Qi S; Luo H. 2012. T cell-specific deletion of EFNB2 minimally affects T cell development and function. Mol Immunol 52(3-4):141-7. [PubMed: 22673212] [MGI Ref ID J:188166]
Luo H; Charpentier T; Wang X; Qi S; Han B; Wu T; Terra R; Lamarre A; Wu J. 2011. Efnb1 and efnb2 proteins regulate thymocyte development, peripheral T cell differentiation, and antiviral immune responses and are essential for interleukin-6 (IL-6) signaling. J Biol Chem 286(48):41135-52. [PubMed: 21976681] [MGI Ref ID J:178148]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
|
|
|
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form
Terms of Use
Terms of Use
General Terms and Conditions
For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
Contact information
General inquiries regarding Terms of UseContracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
No Liability
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.