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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names B6(CBA)-Tg(Lck-cre)I540Jxm/J (Changed: 02-APR-07 ) Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation ?pN1 Donating Investigator Jamey Marth, University of California at San Diego Description
Homozygous mice are viable and have no major defects. This transgenic strain expresses Cre recombinase in thymocytes at lower abundance than line 548-O (Stock No. 003802), resulting in sub-stoichiometric recombination and yielding chimeric cell populations in vivo. In contrast, Lck-Cre transgenic line 548-O recombines loxP-flanked alleles specifically in thymocytes at very high efficiency, up to 99+% as compared with other Lck-Cre transgenic lines.Development
The Cre coding sequence derived from pMC-Cre was cloned into p1017. The transgene was isolated from the plasmid and microinjected into the male pronuclei of B6CBAF2 hybrid zygotes prior to implantation into CD1 foster mothers. A founder expressing the transgene was backcrossed to C57BL/6 by the donating laboratory 9-12 times.
Related Strains
Strains carrying other alleles of Lck
002817 B6.129S2-Lcktm1Mak/J 003802 B6.Cg-Tg(Lck-cre)548Jxm/J 002427 C3H/He-Tg(LCKprBCL2)36Sjk/J 008842 C57BL/6-Tg(Lck-VIPR2)1Ejg/J 003332 C57BL/6-Tg(LckNotch1)9Erob/J 002230 C57BL/6J-Tg(LckIl4)1315Dbl/J 005732 NOD.Cg-Tg(Lck-cre)548Jxm/AchJ View Strains carrying other alleles of Lck (7 strains)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox System
Cre Recombinase Expression
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Lck-cre)I540Jxm | ||
|---|---|---|---|
| Allele Name | transgene insertion I540, Jamey Marth | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Strain of Origin | (C57BL/6 x CBA)F2 | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Lck, lymphocyte protein tyrosine kinase, mouse, laboratory | ||
| Driver Note | Lck | ||
| Molecular Note | The Cre recombinase coding sequence, modified by addition of a Kozak consensus translation start sequence and nuclear localization signals, was inserted into the p1017 expression vector between the thymocyte-specific, mouse proximal Lck promoter and mostof the human growth hormone gene, extending from within exon 1 through the polyadenylation signal. Cre recombinase activity is restricted to thymocytes; evidence of activity in spleen is attributed to the presence of thymus-derived T lymphocytes. [MGI Ref ID J:67674] [MGI Ref ID J:81268] | ||
| Gene Symbol and Name | Tg(Lck-cre)I540Jxm, transgene insertion I540, Jamey Marth | ||
| Chromosome | UN | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(Lck-cre)I540Jxm, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Hennet T; Hagen FK; Tabak LA; Marth JD. 1995. T-cell-specific deletion of a polypeptide N-acetylgalactosaminyl-transferase gene by site-directed recombination. Proc Natl Acad Sci U S A 92(26):12070-4. [PubMed: 8618846] [MGI Ref ID J:67674]
Additional References
Tg(Lck-cre)I540Jxm relatedChaffin KE; Beals CR; Wilkie TM; Forbush KA; Simon MI; Perlmutter RM. 1990. Dissection of thymocyte signaling pathways by in vivo expression of pertussis toxin ADP-ribosyltransferase. EMBO J 9(12):3821-9. [PubMed: 2123451] [MGI Ref ID J:81268]
Health & husbandry
Health & Colony Maintenance Information
Currently there no information available for this strain. This may be due to the supply level of this strain.
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Animals Provided
Price (US dollars $) Cryorecovery Fee $1900.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
| Pricing for International shipping destinations |
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Animals Provided
Price (US dollars $) Cryorecovery Fee $2470.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
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Supply Details
| Standard Supply | Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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Payment Terms and Conditions
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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Contact information
General inquiries
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