Strain Name:

B6.129-St8sia2tm1Jxm/J

Stock Number:

006902

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Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Additional information on Congenic nomenclature.
Specieslaboratory mouse
GenerationN6pN1
Generation Definitions
 
Donating Investigator Jamey D Marth,   Burnham Inst at Univ Calif Santa Barbara

Description
Heterozygous mice are viable and fertile and do not display any gross physical or behavioral abnormalities. Homozygous mice show a polysialic acid (PSA) deficit in regions of neurogenesis. Hippocampal synaptic plasticity is not impaired, but abnormal trafficking of infrapyramidal mossy fibers and the formation of ectopic synapses in the hippocampus are observed coincident with a high exploratory drive and reduced behavioral responses to Pavlovian fear conditioning. This mutant mouse strain represents a model of polysialyltransferase function in modulating axonal trafficking and mechanisms of anxiety and fear behaviors.

Development
A targeting vector was used to place a floxed neomycin-thymidine kinase expression cassette in the intron upstream of exon 4 and a lox P site in the intron downstream of exon 4. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl +-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were transfected with a Cre expression plasmid to excise the floxed regions. Gancyclovir-resistant ES cells were injected into C57BL/6 blastocysts. Chimeric animals were crossed with C57BL/6 at least 6 generations by the donating laboratory.

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

St8sia2tm1Jxm/St8sia2tm1Jxm

        involves: C57BL/6
  • behavior/neurological phenotype
  • abnormal contextual conditioning behavior
    • impaired performance in a contextual fear conditioning test is seen   (MGI Ref ID J:91777)
  • abnormal cued conditioning behavior
    • impaired performance in a cued fear conditioning test is seen, however, the acoustic startle response is normal   (MGI Ref ID J:91777)
  • abnormal passive avoidance behavior
    • mutants fail to increase their latency to enter the dark chamber in a passive avoidance task   (MGI Ref ID J:91777)
  • increased exploration in new environment
    • mutants are more active in the open field test, spend more time in the center of the field, and rear more frequently, however, mutants are not hyperactive   (MGI Ref ID J:91777)
  • nervous system phenotype
  • abnormal hippocampus morphology
    • abnormal mossy fibers on the ventral side of the granule cell layer are associated with ectopic synapses   (MGI Ref ID J:91777)
    • abnormal hippocampal mossy fiber morphology
      • the infrapyramidal mossy fibers extend into the faeCA3a region and into the CA3b and CA3c regionsfrom the age of 2 weeks on   (MGI Ref ID J:91777)
      • mossy fibers also extend along the ventral side of the granule cell layer   (MGI Ref ID J:91777)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Neurobiology Research
Behavioral and Learning Defects
Neurodevelopmental Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol St8sia2tm1Jxm
Allele Name targeted mutation 1, Jamey Marth
Allele Type Targeted (Null/Knockout)
Common Name(s) St8sia-II-;
Mutation Made By Jamey Marth,   Burnham Inst at Univ Calif Santa Barbara
Gene Symbol and Name St8sia2, ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2
Chromosome 7
Gene Common Name(s) AI323367; HsT19690; SIAT8B; ST8SIA-II; ST8SiaII; STX; Siat8b; expressed sequence AI323367; sialyltransferase 8 (alpha-2, 8-sialyltransferase) B;
Molecular Note Exon 4, encoding a significant portion of the sailyl motif L, was deleted upon Cre mediated deletion of the floxed region including the exon and a tk-neo cassette. Southern blot confirmed recombination and RT-PCR indicated that the truncated form of mRNA was produced in heterozygous and homozygous mutants. The mutant cDNA was found to be inactive. [MGI Ref ID J:91777]

Genotyping

Genotyping Information

Genotyping Protocols

St8sia2tm1Jxm, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Angata K; Long JM; Bukalo O; Lee W; Dityatev A; Wynshaw-Boris A; Schachner M; Fukuda M; Marth JD. 2004. Sialyltransferase ST8Sia-II assembles a subset of polysialic acid that directs hippocampal axonal targeting and promotes fear behavior. J Biol Chem 279(31):32603-13. [PubMed: 15140899]  [MGI Ref ID J:91777]

Additional References

St8sia2tm1Jxm related

Angata K; Huckaby V; Ranscht B; Terskikh A; Marth JD; Fukuda M. 2007. Polysialic acid-directed migration and differentiation of neural precursors are essential for mouse brain development. Mol Cell Biol 27(19):6659-68. [PubMed: 17682066]  [MGI Ref ID J:126687]

Calandreau L; Marquez C; Bisaz R; Fantin M; Sandi C. 2010. Differential impact of polysialyltransferase ST8SiaII and ST8SiaIV knockout on social interaction and aggression. Genes Brain Behav 9(8):958-67. [PubMed: 20659171]  [MGI Ref ID J:178208]

Drake PM; Nathan JK; Stock CM; Chang PV; Muench MO; Nakata D; Reader JR; Gip P; Golden KP; Weinhold B; Gerardy-Schahn R; Troy FA 2nd; Bertozzi CR. 2008. Polysialic acid, a glycan with highly restricted expression, is found on human and murine leukocytes and modulates immune responses. J Immunol 181(10):6850-8. [PubMed: 18981104]  [MGI Ref ID J:140945]

Galuska SP; Oltmann-Norden I; Geyer H; Weinhold B; Kuchelmeister K; Hildebrandt H; Gerardy-Schahn R; Geyer R; Muhlenhoff M. 2006. Polysialic acid profiles of mice expressing variant allelic combinations of the polysialyltransferases ST8SiaII and ST8SiaIV. J Biol Chem 281(42):31605-15. [PubMed: 16940046]  [MGI Ref ID J:117398]

Nacher J; Guirado R; Varea E; Alonso-Llosa G; Rockle I; Hildebrandt H. 2010. Divergent impact of the polysialyltransferases ST8SiaII and ST8SiaIV on polysialic acid expression in immature neurons and interneurons of the adult cerebral cortex. Neuroscience 167(3):825-37. [PubMed: 20206239]  [MGI Ref ID J:161492]

Oltmann-Norden I; Galuska SP; Hildebrandt H; Geyer R; Gerardy-Schahn R; Geyer H; Muhlenhoff M. 2008. Impact of the polysialyltransferases ST8SiaII and ST8SiaIV on polysialic acid synthesis during postnatal mouse brain development. J Biol Chem 283(3):1463-71. [PubMed: 18045870]  [MGI Ref ID J:130718]

Rollenhagen M; Buettner FF; Reismann M; Jirmo AC; Grove M; Behrens GM; Gerardy-Schahn R; Hanisch FG; Muhlenhoff M. 2013. Polysialic acid on neuropilin-2 is exclusively synthesized by the polysialyltransferase ST8SiaIV and attached to mucin-type o-glycans located between the b2 and c domain. J Biol Chem 288(32):22880-92. [PubMed: 23801331]  [MGI Ref ID J:202712]

Rollenhagen M; Kuckuck S; Ulm C; Hartmann M; Galuska SP; Geyer R; Geyer H; Muhlenhoff M. 2012. Polysialylation of the synaptic cell adhesion molecule 1 (SynCAM 1) depends exclusively on the polysialyltransferase ST8SiaII in vivo. J Biol Chem 287(42):35170-80. [PubMed: 22908220]  [MGI Ref ID J:191893]

Schiff M; Rockle I; Burkhardt H; Weinhold B; Hildebrandt H. 2011. Thalamocortical pathfinding defects precede degeneration of the reticular thalamic nucleus in polysialic Acid-deficient mice. J Neurosci 31(4):1302-12. [PubMed: 21273415]  [MGI Ref ID J:168547]

Schiff M; Weinhold B; Grothe C; Hildebrandt H. 2009. NCAM and polysialyltransferase profiles match dopaminergic marker gene expression but polysialic acid is dispensable for development of the midbrain dopamine system. J Neurochem 110(5):1661-73. [PubMed: 19619134]  [MGI Ref ID J:152222]

Stoenica L; Senkov O; Gerardy-Schahn R; Weinhold B; Schachner M; Dityatev A. 2006. In vivo synaptic plasticity in the dentate gyrus of mice deficient in the neural cell adhesion molecule NCAM or its polysialic acid. Eur J Neurosci 23(9):2255-64. [PubMed: 16706834]  [MGI Ref ID J:108923]

Weinhold B; Seidenfaden R; Rockle I; Muhlenhoff M; Schertzinger F; Conzelmann S; Marth JD; Gerardy-Schahn R; Hildebrandt H. 2005. Genetic ablation of polysialic acid causes severe neurodevelopmental defects rescued by deletion of the neural cell adhesion molecule. J Biol Chem 280(52):42971-7. [PubMed: 16267048]  [MGI Ref ID J:105899]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2140.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2782.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

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