Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N6pN1 Generation Definitions   Donating Investigator Jamey D Marth,   Burnham Inst at Univ Calif Santa Barbara

Description
Heterozygous mice are viable and fertile and do not display any gross physical or behavioral abnormalities. Homozygous mice show a polysialic acid (PSA) deficit in regions of neurogenesis. Hippocampal synaptic plasticity is not impaired, but abnormal trafficking of infrapyramidal mossy fibers and the formation of ectopic synapses in the hippocampus are observed coincident with a high exploratory drive and reduced behavioral responses to Pavlovian fear conditioning. This mutant mouse strain represents a model of polysialyltransferase function in modulating axonal trafficking and mechanisms of anxiety and fear behaviors.

Development
A targeting vector was used to place a floxed neomycin-thymidine kinase expression cassette in the intron upstream of exon 4 and a lox P site in the intron downstream of exon 4. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl ⁺-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were transfected with a Cre expression plasmid to excise the floxed regions. Gancyclovir-resistant ES cells were injected into C57BL/6 blastocysts. Chimeric animals were crossed with C57BL/6 at least 6 generations by the donating laboratory.

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

St8sia2^tm1Jxm/St8sia2^tm1Jxm

        involves: C57BL/6

• behavior/neurological phenotype
• abnormal contextual conditioning behavior
  • impaired performance in a contextual fear conditioning test is seen   (MGI Ref ID J:91777)
• abnormal cued conditioning behavior
  • impaired performance in a cued fear conditioning test is seen, however, the acoustic startle response is normal   (MGI Ref ID J:91777)
• abnormal passive avoidance behavior
  • mutants fail to increase their latency to enter the dark chamber in a passive avoidance task   (MGI Ref ID J:91777)
• increased exploration in new environment
  • mutants are more active in the open field test, spend more time in the center of the field, and rear more frequently, however, mutants are not hyperactive   (MGI Ref ID J:91777)
• nervous system phenotype
• abnormal hippocampus morphology
  • abnormal mossy fibers on the ventral side of the granule cell layer are associated with ectopic synapses   (MGI Ref ID J:91777)
• • abnormal hippocampal mossy fiber morphology
    • the infrapyramidal mossy fibers extend into the faeCA3a region and into the CA3b and CA3c regionsfrom the age of 2 weeks on   (MGI Ref ID J:91777)
    • mossy fibers also extend along the ventral side of the granule cell layer   (MGI Ref ID J:91777)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Neurobiology Research
Behavioral and Learning Defects
Neurodevelopmental Defects

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		St8sia2tm1Jxm
Allele Name		targeted mutation 1, Jamey Marth
Allele Type		Targeted (knock-out)
Common Name(s)		St8sia-II-;
Mutation Made By		Jamey Marth,   Burnham Inst at Univ Calif Santa Barbara
Gene Symbol and Name		St8sia2, ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2
Chromosome		7
Gene Common Name(s)		AI323367; HsT19690; SIAT8B; ST8SIA-II; ST8SiaII; STX; Siat8b; expressed sequence AI323367; sialyltransferase 8 (alpha-2, 8-sialyltransferase) B;
Molecular Note		Exon 4, encoding a significant portion of the sailyl motif L, was deleted upon Cre mediated deletion of the floxed region including the exon and a tk-neo cassette. Southern blot confirmed recombination and RT-PCR indicated that the truncated form of mRNA was produced in heterozygous and homozygous mutants. The mutant cDNA was found to be inactive. [MGI Ref ID J:91777]

Genotyping

Genotyping Information

Genotyping Protocols

St8sia2^tm1Jxm, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Angata K; Long JM; Bukalo O; Lee W; Dityatev A; Wynshaw-Boris A; Schachner M; Fukuda M; Marth JD. 2004. Sialyltransferase ST8Sia-II assembles a subset of polysialic acid that directs hippocampal axonal targeting and promotes fear behavior. J Biol Chem 279(31):32603-13. [PubMed: 15140899]  [MGI Ref ID J:91777]

Additional References

St8sia2^tm1Jxm related

Angata K; Huckaby V; Ranscht B; Terskikh A; Marth JD; Fukuda M. 2007. Polysialic acid-directed migration and differentiation of neural precursors are essential for mouse brain development. Mol Cell Biol 27(19):6659-68. [PubMed: 17682066]  [MGI Ref ID J:126687]

Calandreau L; Marquez C; Bisaz R; Fantin M; Sandi C. 2010. Differential impact of polysialyltransferase ST8SiaII and ST8SiaIV knockout on social interaction and aggression. Genes Brain Behav 9(8):958-67. [PubMed: 20659171]  [MGI Ref ID J:178208]

Drake PM; Nathan JK; Stock CM; Chang PV; Muench MO; Nakata D; Reader JR; Gip P; Golden KP; Weinhold B; Gerardy-Schahn R; Troy FA 2nd; Bertozzi CR. 2008. Polysialic acid, a glycan with highly restricted expression, is found on human and murine leukocytes and modulates immune responses. J Immunol 181(10):6850-8. [PubMed: 18981104]  [MGI Ref ID J:140945]

Galuska SP; Oltmann-Norden I; Geyer H; Weinhold B; Kuchelmeister K; Hildebrandt H; Gerardy-Schahn R; Geyer R; Muhlenhoff M. 2006. Polysialic acid profiles of mice expressing variant allelic combinations of the polysialyltransferases ST8SiaII and ST8SiaIV. J Biol Chem 281(42):31605-15. [PubMed: 16940046]  [MGI Ref ID J:117398]

Nacher J; Guirado R; Varea E; Alonso-Llosa G; Rockle I; Hildebrandt H. 2010. Divergent impact of the polysialyltransferases ST8SiaII and ST8SiaIV on polysialic acid expression in immature neurons and interneurons of the adult cerebral cortex. Neuroscience 167(3):825-37. [PubMed: 20206239]  [MGI Ref ID J:161492]

Oltmann-Norden I; Galuska SP; Hildebrandt H; Geyer R; Gerardy-Schahn R; Geyer H; Muhlenhoff M. 2008. Impact of the polysialyltransferases ST8SiaII and ST8SiaIV on polysialic acid synthesis during postnatal mouse brain development. J Biol Chem 283(3):1463-71. [PubMed: 18045870]  [MGI Ref ID J:130718]

Rollenhagen M; Kuckuck S; Ulm C; Hartmann M; Galuska SP; Geyer R; Geyer H; Muhlenhoff M. 2012. Polysialylation of the synaptic cell adhesion molecule 1 (SynCAM 1) depends exclusively on the polysialyltransferase ST8SiaII in vivo. J Biol Chem 287(42):35170-80. [PubMed: 22908220]  [MGI Ref ID J:191893]

Schiff M; Rockle I; Burkhardt H; Weinhold B; Hildebrandt H. 2011. Thalamocortical pathfinding defects precede degeneration of the reticular thalamic nucleus in polysialic Acid-deficient mice. J Neurosci 31(4):1302-12. [PubMed: 21273415]  [MGI Ref ID J:168547]

Schiff M; Weinhold B; Grothe C; Hildebrandt H. 2009. NCAM and polysialyltransferase profiles match dopaminergic marker gene expression but polysialic acid is dispensable for development of the midbrain dopamine system. J Neurochem 110(5):1661-73. [PubMed: 19619134]  [MGI Ref ID J:152222]

Stoenica L; Senkov O; Gerardy-Schahn R; Weinhold B; Schachner M; Dityatev A. 2006. In vivo synaptic plasticity in the dentate gyrus of mice deficient in the neural cell adhesion molecule NCAM or its polysialic acid. Eur J Neurosci 23(9):2255-64. [PubMed: 16706834]  [MGI Ref ID J:108923]

Weinhold B; Seidenfaden R; Rockle I; Muhlenhoff M; Schertzinger F; Conzelmann S; Marth JD; Gerardy-Schahn R; Hildebrandt H. 2005. Genetic ablation of polysialic acid causes severe neurodevelopmental defects rescued by deletion of the neural cell adhesion molecule. J Biol Chem 280(52):42971-7. [PubMed: 16267048]  [MGI Ref ID J:105899]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

See Terms of Use tab for General Terms and Conditions

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

---

Ordering Information
JAX^® Mice
Surgical and Preconditioning Services
JAX^® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.