Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation ?+N1F4pN1 Generation Definitions   Donating Investigator Rhonda Bassel-Duby,   University of Texas Southwestern

Description
Mice homozygous for this MyoR mutant allele are viable and fertile with no obvious abnormalities. These mice may be useful in studying muscle development, specifically craniofacial muscles.

For example, when these mice are bred with capsulin-mutant mice, the resulting double mutant offspring have significant abnormalities in craniofacial (and other) muscle development and MyoD-family transcription factor gene expression.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Development
A targeting vector was designed replace the coding region within the first exon of the targeted gene with a neomyocin-resistance gene in the opposite orientation. The construct was electroporated into 129-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Chimeric animals were bred to generate the mutant colony on a 129 genetic background. At some point, these mice were bred with C57BL/6 mice. Homozygous mice on a mixed C57BL/6 and 129 genetic background were bred together for many generations prior to arrival at The Jackson Laboratory.

Control Information

	Control
	101043 B6129SF1/J	(approximate)
	101045 B6129SF2/J	(approximate)
Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Msc^tm1Eno/Msc^tm1Eno

        involves: 129

• normal phenotype
• no abnormal phenotype detected
  • homozygotes are obtained at the expected Mendelian ratios and show no obvious abnormalities   (MGI Ref ID J:111337)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Cell Biology Research
Transcriptional Regulation

Developmental Biology Research
Craniofacial and Palate Defects
Mesodermal Defects
      Myogenesis Defects

Research Tools
Developmental Biology Research

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Msctm1Eno
Allele Name		targeted mutation 1, Eric N Olson
Allele Type		Targeted (knock-out)
Common Name(s)		MyoR-;
Mutation Made By		Eric Olson,   University of Texas Southwestern Medical
Strain of Origin		129
ES Cell Line Strain		129
Gene Symbol and Name		Msc, musculin
Chromosome		1
Gene Common Name(s)		ABF-1; ABF1; MYOR; bHLHa22;
Molecular Note		Gene targeting resulted in deletion of the coding region within the first exon and introduced a neomyocin-resistance gene in the opposite orientation to the gene. [MGI Ref ID J:111337]

Genotyping

Genotyping Information

Genotyping Protocols

Generic Neo Melt Curve Analysis, Melt Curve Analysis
Generic Neo Melt Curve Analysis, Robotic MCA
Msc^tm1Eno, Separated PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Lu JR; Bassel-Duby R; Hawkins A; Chang P; Valdez R; Wu H; Gan L; Shelton JM; Richardson JA; Olson EN. 2002. Control of facial muscle development by MyoR and capsulin. Science 298(5602):2378-81. [PubMed: 12493912]  [MGI Ref ID J:111337]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, RG10/RG30.

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, homozygous mice are bred.

Purchasing information

Pricing, Supply Level & Notes, Controls

General Supply Notes

• This strain is included in the Induced Mutant Resource Colony collection.
• Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	101043 B6129SF1/J	(approximate)
	101045 B6129SF2/J	(approximate)
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

See Terms of Use tab for General Terms and Conditions

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering Information
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Terms of Use

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Contact information

General inquiries

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phone:	207-288-6470
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JAX^® Mice, Products & Services Conditions of Use

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