Strain Name:

B6;CBA-Tg(APOC3)3707Bres/J

Stock Number:

006907

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Availability:

Cryopreserved - Ready for recovery

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
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Specieslaboratory mouse
Generation?+F1pN1
Generation Definitions
 
Donating InvestigatorDr. Jan L. Breslow,   Rockefeller University

Description
Mice hemizygous for this "human apo CIII" transgene are viable and fertile. This high expressor founder line (3703) exhibits transgene expression primarily in the liver with some intestinal expression as well. Hemizygous mice have severe plasma hypertriglyceridemia and significantly increased plasma cholesterol. However, resistance to insulin-mediated glucose uptake or hyperinsulinemia are not observed. This high expressor human apo CIII transgenic strain may be useful in studying lipid metabolism, very low density lipoproteins (VLDL), hypertriglyceridemia, coronary heart disease, and/or atherosclerosis.

Development
A transgenic construct containing the human apolipoprotein C-III gene (including 2.5 kb 5' and 1.1 kb 3' flanking sequences) was injected into the male pronucleus of fertilized eggs (both from (C57BL/6J x CBA/J)F1 mice). The resulting animals were bred to (C57BL/6J x CBA/J)F1 to establish founder line 3707. Transgenic mice were bred to wildtype siblings for many generations prior to arrival at The Jackson Laboratory. The donating investigator reports that physical location of transgene insertion(s) are unknown, but given the high expression of the transgene, multiple copies are likely.

Control Information

  Control
   Noncarrier
   100011 B6CBAF1/J
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on transgenic expression of an ortholog of a human gene that is associated with this disease. Phenotypic similarity to the human disease has not been tested.
Hyperalphalipoproteinemia 2; HALP2   (APOC3)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Tg(APOC3)3707Bres/0

        involves: C57BL/6J * CBA/J
  • homeostasis/metabolism phenotype
  • *normal* homeostasis/metabolism phenotype
    • the mean fasting plasma glucose concentration is essentially identical in transgenic mice and littermate controls   (MGI Ref ID J:119638)
    • the mean fasting plasma insulin concentration is essentially identical in transgenic mice and littermate controls   (MGI Ref ID J:119638)
    • insulin-mediated glucose uptake does not differ between transgenic mice and littermate controls, based on their attaining similar steady-state plasma glucose (SSPG) and steady-state plasma insulin (SSPI) levels following co-infusion of glucose (14.5 mg/min per g body weight) and insulin (0.2 ng/min per gram body weight)   (MGI Ref ID J:119638)
    • increased circulating cholesterol level
      • the mean fasting cholesterol is significantly higher in 7-8-month old transgenic mice (3.5 +/- 0.2 mmol/l) than in littermate controls (2.4 +/- mmol/l) (P<0.001)   (MGI Ref ID J:119638)
    • increased circulating triglyceride level
      • the mean fasting plasma triglyceride concentration is considerably higher in 7-8-month old transgenic mice (11.8 +/- 0.2 mmol/l) than in littermate controls (1.1 +/- 0.1 mmol/l) (P<0.001)   (MGI Ref ID J:119638)
  • adipose tissue phenotype
  • *normal* adipose tissue phenotype
    • epididymal fat pad adipocytes isolated from transgenic and control mice exhibit similar glucose transport rates at a maximal insulin concentration (Vmax)   (MGI Ref ID J:119638)
    • the insulin concentration inducing half-maximal glucose transport rate (ED50) is similar in epididymal fat pad adipocytes isolated from transgenic and control mice   (MGI Ref ID J:119638)
    • isoproterenol-induced lipolysis, measured by glycerol release, is essentially identical in transgenic and control epididymal fat pad adipocytes over a dose range of 10-9-10-6   (MGI Ref ID J:119638)
    • insulin inhibition of isoproterenol-induced lipolysis, as measured by glycerol release, is essentially identical in transgenic and control epididymal fat pad adipocytes   (MGI Ref ID J:119638)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cardiovascular Research
Atherosclerosis
Hypercholesterolemia
Hypertriglyceridemia
Other
      altered lipoprotein profile

Diabetes and Obesity Research
Obesity Without Diabetes

Metabolism Research
Lipid Metabolism

Research Tools
Cardiovascular Research
Diabetes and Obesity Research
Metabolism Research

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tg(APOC3)3707Bres
Allele Name transgene insertion 3707, Jan L Breslow
Allele Type Transgenic (random, expressed)
Common Name(s) HuCIII Tg;
Mutation Made ByDr. Jan Breslow,   Rockefeller University
Strain of Origin(C57BL/6J x CBA/J)F1
Expressed Gene APOC3, apolipoprotein C-III, human
Promoter APOC3, apolipoprotein C-III, human
Molecular Note Male pronuclei of fertilized oocytes were microinjected with a transgenic construct comprising the human apolipoprotein C-III gene, including 2.5 kb of 5' and 1.1 kb of 3' flanking sequence. Mice of this line express the transgene at high levels in the liver and secondarily in the intestine. [MGI Ref ID J:81184]
 

Genotyping

Genotyping Information

Genotyping Protocols

Tg(APOC3)3707Bres, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Reaven GM; Mondon CE; Chen YD; Breslow JL. 1994. Hypertriglyceridemic mice transgenic for the human apolipoprotein C-III gene are neither insulin resistant nor hyperinsulinemic. J Lipid Res 35(5):820-4. [PubMed: 8071604]  [MGI Ref ID J:119638]

Additional References

Tg(APOC3)3707Bres related

Alberici LC; Oliveira HC; Paim BA; Mantello CC; Augusto AC; Zecchin KG; Gurgueira SA; Kowaltowski AJ; Vercesi AE. 2009. Mitochondrial ATP-sensitive K(+) channels as redox signals to liver mitochondria in response to hypertriglyceridemia. Free Radic Biol Med 47(10):1432-9. [PubMed: 19703550]  [MGI Ref ID J:155016]

Alberici LC; Oliveira HC; Patricio PR; Kowaltowski AJ; Vercesi AE. 2006. Hyperlipidemic mice present enhanced catabolism and higher mitochondrial ATP-sensitive K+ channel activity. Gastroenterology 131(4):1228-34. [PubMed: 17030192]  [MGI Ref ID J:147323]

Amaral ME; Oliveira HC; Carneiro EM; Delghingaro-Augusto V; Vieira EC; Berti JA; Boschero AC. 2002. Plasma glucose regulation and insulin secretion in hypertriglyceridemic mice. Horm Metab Res 34(1):21-6. [PubMed: 11832997]  [MGI Ref ID J:74962]

Conde-Knape K; Okada K; Ramakrishnan R; Shachter NS. 2004. Overexpression of apoC-III produces lesser hypertriglyceridemia in apoB-48-only gene-targeted mice than in apoB-100-only mice. J Lipid Res 45(12):2235-44. [PubMed: 15342689]  [MGI Ref ID J:94159]

Ito Y; Azrolan N; O'Connell A; Walsh A; Breslow JL. 1990. Hypertriglyceridemia as a result of human apo CIII gene expression in transgenic mice. Science 249(4970):790-3. [PubMed: 2167514]  [MGI Ref ID J:81184]

Masucci-Magoulas L; Goldberg IJ; Bisgaier CL; Serajuddin H; Francone OL ; Breslow JL ; Tall AR. 1997. A mouse model with features of familial combined hyperlipidemia. Science 275(5298):391-4. [PubMed: 8994037]  [MGI Ref ID J:37861]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, hemizygous mice are bred to wildtype siblings or to B6CBAF1/J (Stock No. 100011).

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2085.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2710.50
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Noncarrier
   100011 B6CBAF1/J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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