Strain Name: |
B6.Cg-Ikbketm1Tman/J |
|---|---|
Stock Number: |
006908 |
Availability: | Repository- Live |
General Terms and Conditions |
| Genes & Alleles | Ikbke; Ikbketm1Tman; |
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Donating Investigator Tom Maniatis, Harvard University Generation N5+F?F2 (08-JAN-08) Strain Description
Homozygous IKKepsilon mutant mice are viable and fertile. Homozygous deletion results in a complete loss of the endogenous kinase function in lung, spleen, and embryonic fibroblasts. These mice have increased susceptibility to viral infection due to defective inteferon (IFN) signaling. These IKKepsilon mutant mice may be useful in immunological studies involving IFN signaling and host responses to infection.Strain Development
The donating investigator reports that a targeting vector was used to replace exons 4-6 of the targeted gene with a PGK-neo cassette. The construct was electroporated into "B6/S129"-hybrid derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts, and chimeric offspring were bred to C57BL/6J mice. Heterozygous mutants were bred to inbred C57BL/6J mice for at least 5 generations prior to arrival at The Jackson Laboratory.
Mammalian Phenotype Terms assigned by genotype |
| Allele Symbol | Ikbketm1Tman | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Tom Maniatis | ||
| Common Name(s) | Ikbke-; | ||
| Mutation Made By | Tom Maniatis, Harvard University | ||
| Strain of Origin | 129 and C57BL/6 | ||
| ES Cell Line Strain | 129 | ||
| Gene Symbol and Name | Ikbke, inhibitor of kappaB kinase epsilon | ||
| Chromosome | 1 | ||
| Gene Common Name(s) | AW558201; IKK-i; IKKE; IKKI; IKKepsilon; KIAA0151; MGC125294; MGC125295; MGC125297; expressed sequence AW558201; | ||
| Molecular Note | Exons 4-6 of the targeted gene were replaced with a PGK-neo cassette. Mice show a complete loss of the endogenous kinase function in lung, spleen, and embryonic fibroblasts. [MGI Ref ID J:119791] | ||
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Ikbketm1Tman
NEOTD (Generic Neo)
| Breeding & Husbandry | When maintaining a live colony, homozygous mice may be bred together. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Congenic Nomenclature
Room Number AX11
Cancer Research
Growth Factors/Receptors/Cytokines
Cell Biology Research
Signal Transduction
Hematological Research
Immunological Defects
Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers (genes regulating susceptibility to infectious disease and endotoxin)
Growth Factors/Receptors/Cytokines
Immunodeficiency (multiple immune defects)
Intracellular Signaling Molecules
Lymphoid Tissue Defects
Research Tools
Immunology and Inflammation Research (genes regulating susceptibility to infectious disease and endotoxin)
Virology Research
Selected Reference(s)
Tenoever BR; Ng SL; Chua MA; McWhirter SM; Garcia-Sastre A; Maniatis T. 2007. Multiple functions of the IKK-related kinase IKKepsilon in interferon-mediated antiviral immunity. Science 315(5816):1274-8. [PubMed: 17332413] [MGI Ref ID J:119791]
| Strain Name: | B6.Cg-Ikbketm1Tman/J |
| Stock Number: | 006908 |
IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
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