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Genes & Alleles

Ikbke;   Ikbketm1Tman;

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Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Mating System Homozygote x Homozygote         (Female x Male) Species laboratory mouse Donating Investigator Tom Maniatis,   Harvard University Generation N5+F?F2 (08-JAN-08)

Strain Description
Homozygous IKKepsilon mutant mice are viable and fertile. Homozygous deletion results in a complete loss of the endogenous kinase function in lung, spleen, and embryonic fibroblasts. These mice have increased susceptibility to viral infection due to defective inteferon (IFN) signaling. These IKKepsilon mutant mice may be useful in immunological studies involving IFN signaling and host responses to infection.

Strain Development
The donating investigator reports that a targeting vector was used to replace exons 4-6 of the targeted gene with a PGK-neo cassette. The construct was electroporated into "B6/S129"-hybrid derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts, and chimeric offspring were bred to C57BL/6J mice. Heterozygous mutants were bred to inbred C57BL/6J mice for at least 5 generations prior to arrival at The Jackson Laboratory.

Mammalian Phenotype Terms assigned by genotype Ikbketm1Tman/Ikbketm1Tman         B6.Cg-Ikbketm1Tman immune system phenotype increased susceptibility to viral infection (MGI Ref ID J:119791) while at inoculations of >7 x103 pfu, mutant and control mice show 100% mortality, mutants show greater susceptibility to infection by influenza at lower titers than control mice in infected primary mouse embryonic fibroblasts, rates of virus replication are similar to wild-type, while the titer continues to increase 7 days post-infection, lungs of mutants show increased immunopathology compared to wild-type lung inflammation (MGI Ref ID J:119791) 7 days post infection, mutant lungs display inflammatory infiltrate consisting of lymphocytes, macrophages, and neutrophils growth/size phenotype weight loss (MGI Ref ID J:119791) after inoculation with 700 pfu, both mutants and controls show a drop of >10% body weight, but mutants continue to lose weight to below 80% of their original body mass before mortality, whereas control mice do not respiratory system phenotype lung inflammation (MGI Ref ID J:119791) 7 days post infection, mutant lungs display inflammatory infiltrate consisting of lymphocytes, macrophages, and neutrophils cellular phenotype abnormal cell physiology (MGI Ref ID J:119791) in mutant fibroblasts, about 30% of IFN-stimulated genes (ISGs) are poorly induced by IFNgamma compared to induction in wild-type fibroblasts

Gene & Allele Details

Allele Symbol		Ikbketm1Tman
Allele Name		targeted mutation 1, Tom Maniatis
Common Name(s)		Ikbke-;
Mutation Made By		Tom Maniatis,   Harvard University
Strain of Origin		129 and C57BL/6
ES Cell Line Strain		129
Gene Symbol and Name		Ikbke, inhibitor of kappaB kinase epsilon
Chromosome		1
Gene Common Name(s)		AW558201; IKK-i; IKKE; IKKI; IKKepsilon; KIAA0151; MGC125294; MGC125295; MGC125297; expressed sequence AW558201;
Molecular Note		Exons 4-6 of the targeted gene were replaced with a PGK-neo cassette. Mice show a complete loss of the endogenous kinase function in lung, spleen, and embryonic fibroblasts. [MGI Ref ID J:119791]

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Genotyping Protocols

Ikbke^tm1Tman
NEOTD (Generic Neo)

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, homozygous mice may be bred together.
Diet Information	LabDiet® 5K52/5K67

Additional Web Information

Congenic Nomenclature

Animal Health Reports

Room Number           AX11

Research Applications

This mouse can be used to support research in many areas including:

Cancer Research
Growth Factors/Receptors/Cytokines

Cell Biology Research
Signal Transduction

Hematological Research
Immunological Defects

Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers (genes regulating susceptibility to infectious disease and endotoxin)
Growth Factors/Receptors/Cytokines
Immunodeficiency (multiple immune defects)
Intracellular Signaling Molecules
Lymphoid Tissue Defects

Research Tools
Immunology and Inflammation Research (genes regulating susceptibility to infectious disease and endotoxin)

Virology Research

References

Selected Reference(s)

Tenoever BR; Ng SL; Chua MA; McWhirter SM; Garcia-Sastre A; Maniatis T. 2007. Multiple functions of the IKK-related kinase IKKepsilon in interferon-mediated antiviral immunity. Science 315(5816):1274-8. [PubMed: 17332413]  [MGI Ref ID J:119791]

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Price and Supply Information

Strain Name:	B6.Cg-Ikbketm1Tman/J
Stock Number:	006908

Price Details

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Supply Details

Standard Supply	Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes	Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection.
Licensing	See General Terms and Conditions below for Licensing and Use Restrictions
Control Information	View Control Information in Strain Details.

General Terms and Conditions

View JAX^® Mice & Services Conditions of Use.

For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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