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| These "Oct-4/rtTA" mutant mice may be useful for studies of tumorigenesis and pluripotent stem cells. | |||||||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Former Names B6;129-Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm2(tetO-Pou5f1)Jae/J (Changed: 12-APR-07 ) Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation ?+N1F9 (30-JUN-11)
Generation DefinitionsDonating Investigator Rudolf Jaenisch, Whitehead Institute (MIT) Description
Mice heterozygous for both targeted mutations (R26-rtTA and Cola1a::tetO-Oct4) are viable and fertile. These "Oct-4/rtTA" mice express rtTA-M2, an optimized form of reverse tetracycline-controlled transactivator (rtTA) protein, in multiple tissues. In the absence of the tetracycline analog doxycycline (dox), Oct4 (Pou5f1) expression from the Col1a1 locus is not detected. Following dox administration, high Oct4 expression is induced in liver, bone marrow, stomach, intestine, and skin, with lower levels in the heart, lungs, kidney, spleen, and thymus; no expression was detected in the brain and testes. Dox-inducd activation of Oct4 results in dysplasia in epithelial tissues. These mutant mice may be useful for studies of tumorigenesis and pluripotent cells.Development
A targeting vector was designed to insert an optimized form of reverse tetracycline controlled transactivator (rtTA-M2) followed by a β-globin intron and polyA signal downstream of the Gt(ROSA)26Sor promoter. This construct was electroporated into (C57BL/6 x 129S4Sv/Jae)F1-derived V6.5 embryonic stem (ES) cells. In addition, these ES cells were retargeted to insert an frt-flanked PGK-Neo was into the 3' UTR of the Col1a1 locus. Correctly targeted ES cells were selected and the frt-flanked PGK-Neo in the 3' UTR of the Col1a1 locus was replaced by injecting both a "flip-in plasmid" containing a splice acceptor-double polyA sequence and the tetracycline responsive element (TRE or tetO) upstream of the mouse Oct4 (Pou5f1) cDNA sequence, as well as a Flpe-expressing plasmid to facilitate tetO-Oct4 recombination into the 3' UTR of the Col1a1 locus. The resulting ES cells (now targeted with rtTA-M2 in the Gt(ROSA)26Sor locus and tetO-Oct4 in the Col1a1 locus) were injected into B6D2F1 tetraploid blastocysts. Chimeric mice were bred together for many generations prior to arrival at The Jackson Laboratory.
Control Information
| Control | ||
|---|---|---|
| 101043 B6129SF1/J | (approximate) | |
| 101045 B6129SF2/J | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying Gt(ROSA)26Sortm1(rtTA*M2)Jae allele
View Strains carrying Gt(ROSA)26Sortm1(rtTA*M2)Jae (6 strains)
Additional Web Information
Tet Expression Systems
Phenotype
Phenotype Information
assigned by genotype
Col1a1tm2(tetO-Pou5f1)Jae/Col1a1+ Gt(ROSA)26Sortm1(rtTA*M2)Jae/Gt(ROSA)26Sor+
involves: 129S4/SvJae * C57BL/6
- mortality/aging
- premature death
- digestive/alimentary phenotype
- abnormal intestinal epithelium morphology
- after doxycline treatment, dysplastic cells are found in the entire epithelium; cells have structural and cytological dysplasia which mimics adenocarcinoma (MGI Ref ID J:98920)
- abnormal small intestine morphology
- the proximal part of the small intestine is most severely affected by doxycycline treatment with abnormal cells often almost obstructing the lumen (MGI Ref ID J:98920)
- after 5 days of doxycycline treatment, proliferative zone expands; postmitotic, differentiated cells lining the villus are replaced (MGI Ref ID J:98920)
- upon cessation of treatment, cells migrate to final destinations and differentiate upon cessation of treatment, cells migrate to final destinations and differentiate resulting in restoration of normal morphology (MGI Ref ID J:98920)
- abnormal stomach morphology
- in doxycycline treated mice, cells in the forestomach show a marked atypia and increased mitotic activity (MGI Ref ID J:98920)
- abnormal stomach mucosa morphology
- pyloric mucosa contains lesions resembling high grade-dysplasia in doxycycline treated mice (MGI Ref ID J:98920)
- abnormal intermediate gastric gland morphology
- hyperplastic fundic glands are seen in doxycycline treated mice (MGI Ref ID J:98920)
- abnormal stomach epithelium morphology
- in doxycycline treated mice, forestomach epithelium is thickened and stomach shows lack of differentiation into granular and cornified cell layers compared to control mice (MGI Ref ID J:98920)
- the thickened epithelium consists of atypical cells with enlarged nuclei and prominent nucleoli (MGI Ref ID J:98920)
- abnormal stomach glandular epithelium morphology
- after doxycycline treatment, mice display severe dysplasia and increased proliferation (MGI Ref ID J:98920)
- stomach epithelial hyperplasia
- cells show atypia and increased mitotic activity throughout the squamous epithelial layer in doxycycline treated mice (MGI Ref ID J:98920)
- homeostasis/metabolism phenotype
- dehydration
- after 3-5 days of doxycycline treatment, animals display severe dehydration (MGI Ref ID J:98920)
- cellular phenotype
- abnormal cell proliferation
- behavior/neurological phenotype
- lethargy
- animals become lethargic with doxycycline treatment within 3-5 days (MGI Ref ID J:98920)
- hematopoietic system phenotype
- spleen atrophy
- in doxycycline treated mice (MGI Ref ID J:98920)
- thymus atrophy
- atrophy and absence of CD4, CD8 double positive cells in doxycycline treated mice (MGI Ref ID J:98920)
- immune system phenotype
- spleen atrophy
- in doxycycline treated mice (MGI Ref ID J:98920)
- thymus atrophy
- atrophy and absence of CD4, CD8 double positive cells in doxycycline treated mice (MGI Ref ID J:98920)
- tumorigenesis
- increased skin tumor incidence
- in doxycycline treated mice, tumors originating from the outer-root-sheath progenitors and invading the subcutaneous layer are seen (MGI Ref ID J:98920)
- integument phenotype
- abnormal epidermal layer morphology
- after 5-10 days of doxycycline treatment, mice show mild to moderate epidermal dysplasia with a decrease in differentiation in dysplastic cells (MGI Ref ID J:98920)
- abnormal hair follicle morphology
- increased numbers of immature cells in the hair follicles of the skin are seen after 5-10 days of doxycycline (MGI Ref ID J:98920)
- increased skin tumor incidence
- in doxycycline treated mice, tumors originating from the outer-root-sheath progenitors and invading the subcutaneous layer are seen (MGI Ref ID J:98920)
This mouse can be used to support research in many areas including:
Cancer Research
Other
Cell Biology Research
Transcriptional Regulation
Research Tools
Cancer Research
Tetop Tet System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Tetop Tet System
Mutagenesis and Transgenesis: transcriptional activation
Tet Expression Systems
tTA/rtTA Expressing Strains
tTA/rtTA Responsive Strains
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Col1a1tm2(tetO-Pou5f1)Jae | ||
|---|---|---|---|
| Allele Name | targeted mutation 2, Rudolf Jaenisch | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | Col1a1::TetOP-Oct4; | ||
| Strain of Origin | (C57BL/6 x 129S4/SvJae)F1 | ||
| ES Cell Line Name | v6.5 | ||
| ES Cell Line Strain | (C57BL/6 x 129S4/SvJae)F1 | ||
| Expressed Gene | tetO, tet operator, | ||
| General Note | This mutation is present in the same mouse line carrying the Gt(ROSA)26Sortm1(rtTA*M2)Jae mutation. Expression is induced by doxycycline administration. Mutant ES cell line = C10. | ||
| Molecular Note | ES cells containing the Gt(ROSA)26Sortm1(rtTA2S-M2)Jae mutation were retargeted. Cells were injected with a "flip-in plasmid" containing a tetracycline responsive element (TRE or tetO) and mouse Oct4 (Pou5f1) cDNA sequence (and a Flpe-recombinase plasmid to facilitate tetO-Oct4 integration into the 3' UTR region of Col1a1). With doxycycline treatment, Pou5f1 expression from the Col1a1 locus is widespread with high levels in skin, intestine and stomach, while lower levels are seen in the heart,spleen, thymus and several other organs. No expression is seen in the brain or testes. [MGI Ref ID J:98920] | ||
| Gene Symbol and Name | Col1a1, collagen, type I, alpha 1 | ||
| Chromosome | 11 | ||
| Gene Common Name(s) | Col1a-1; Cola-1; Cola1; Moloney leukemia virus 13; Mov-13; OI4; | ||
| Allele Symbol | Gt(ROSA)26Sortm1(rtTA*M2)Jae | ||
| Allele Name | targeted mutation 1, Rudolf Jaenisch | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | Gt(ROSA)26Sortm1(M2rtTA)Jae; R26-M2rtTA; R26-rtTA; Rosa26-rtRA-nls; | ||
| Mutation Made By | Rudolf Jaenisch, Whitehead Institute (MIT) | ||
| Strain of Origin | (C57BL/6 x 129S4/SvJae)F1 | ||
| ES Cell Line Name | v6.5 | ||
| ES Cell Line Strain | (C57BL/6 x 129S4/SvJae)F1 | ||
| Site of Expression | Expresses an optimized rtTA protein (rtTA-M2). Inducible target gene expression is detected in liver, bone marrow, stomach, intestine, and skin, with lower levels in the heart, lungs, kidney, spleen, and thymus; no expression is detected in the brain and testes. | ||
| Expressed Gene | rtTA, reverse tetracycline-controlled transactivator, E. coli | ||
| The tetracycline repressor gene (Tetr), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). One mutant with a four amino acid residue change (rTetR) exhibited dependence on tetracycline for induction of the targeted gene and was used in the rtTA construct (Gossen et al, 1995). rTetr was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16). | |||
| General Note | This mutation is always used in combination with the Col1a1tm2(tetO-Pou5f1)Jae allele in the same mouse line. Mutant ES cell line = KH2. | ||
| Molecular Note | An optimized form of reverse tetracycline controlled transactivator (rtTA-M2) was inserted downstream of the Gt(ROSA)26Sor promoter. This mutant form of rtTA termed M2 has five amino acid substitutions in the tetR moiety of tTA: S12G, E19G, A56P, D148E and H179R. This mutated form of transactivatory protein has increased doxycycline sensitivity. Mice have widespread expression of the rtTA-M2 protein. [MGI Ref ID J:98920] | ||
| Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | AV258896; Gtrgeo26; Gtrosa26; R26; ROSA26; beta geo; expressed sequence AV258896; gene trap ROSA 26; gene trap ROSA b-geo 26; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Col1a1 3'UTR assay2 (flip-in), Separated PCR
Gt(ROSA)26Sortm1sor STD, Robotic STD
Gt(ROSA)26Sortm1sor STD, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Hochedlinger K; Yamada Y; Beard C; Jaenisch R. 2005. Ectopic expression of Oct-4 blocks progenitor-cell differentiation and causes dysplasia in epithelial tissues. Cell 121(3):465-77. [PubMed: 15882627] [MGI Ref ID J:98920]
Additional References
Col1a1tm2(tetO-Pou5f1)Jae relatedGt(ROSA)26Sortm1(rtTA*M2)Jae relatedJaako P; Flygare J; Olsson K; Quere R; Ehinger M; Henson A; Ellis S; Schambach A; Baum C; Richter J; Larsson J; Bryder D; Karlsson S. 2011. Mice with ribosomal protein S19 deficiency develop bone marrow failure and symptoms like patients with Diamond-Blackfan anemia. Blood 118(23):6087-96. [PubMed: 21989989] [MGI Ref ID J:179085]
Maherali N; Sridharan R; Xie W; Utikal J; Eminli S; Arnold K; Stadtfeld M; Yachechko R; Tchieu J; Jaenisch R; Plath K; Hochedlinger K. 2007. Directly reprogrammed fibroblasts show global epigenetic remodeling and widespread tissue contribution. Cell Stem Cell 1(1):55-70. [PubMed: 18371336] [MGI Ref ID J:158493]
Watanabe S; Hirai H; Asakura Y; Tastad C; Verma M; Keller C; Dutton JR; Asakura A. 2011. MyoD gene suppression by Oct4 is required for reprogramming in myoblasts to produce induced pluripotent stem cells. Stem Cells 29(3):505-16. [PubMed: 21425413] [MGI Ref ID J:175957]
Beard C; Hochedlinger K; Plath K; Wutz A; Jaenisch R. 2006. Efficient method to generate single-copy transgenic mice by site-specific integration in embryonic stem cells. Genesis 44(1):23-8. [PubMed: 16400644] [MGI Ref ID J:159351]
Brambrink T; Foreman R; Welstead GG; Lengner CJ; Wernig M; Suh H; Jaenisch R. 2008. Sequential expression of pluripotency markers during direct reprogramming of mouse somatic cells. Cell Stem Cell 2(2):151-9. [PubMed: 18371436] [MGI Ref ID J:149805]
Brennand K; Huangfu D; Melton D. 2007. All beta Cells Contribute Equally to Islet Growth and Maintenance. PLoS Biol 5(7):e163. [PubMed: 17535113] [MGI Ref ID J:124045]
Carey BW; Markoulaki S; Beard C; Hanna J; Jaenisch R. 2010. Single-gene transgenic mouse strains for reprogramming adult somatic cells. Nat Methods 7(1):56-9. [PubMed: 20010831] [MGI Ref ID J:157298]
Dickins RA; McJunkin K; Hernando E; Premsrirut PK; Krizhanovsky V; Burgess DJ; Kim SY; Cordon-Cardo C; Zender L; Hannon GJ; Lowe SW. 2007. Tissue-specific and reversible RNA interference in transgenic mice. Nat Genet 39(7):914-21. [PubMed: 17572676] [MGI Ref ID J:123006]
Foudi A; Hochedlinger K; Van Buren D; Schindler JW; Jaenisch R; Carey V; Hock H. 2009. Analysis of histone 2B-GFP retention reveals slowly cycling hematopoietic stem cells. Nat Biotechnol 27(1):84-90. [PubMed: 19060879] [MGI Ref ID J:172421]
Gros J; Hu JK; Vinegoni C; Feruglio PF; Weissleder R; Tabin CJ. 2010. WNT5A/JNK and FGF/MAPK pathways regulate the cellular events shaping the vertebrate limb bud. Curr Biol 20(22):1993-2002. [PubMed: 21055947] [MGI Ref ID J:166897]
He S; Kim I; Lim MS; Morrison SJ. 2011. Sox17 expression confers self-renewal potential and fetal stem cell characteristics upon adult hematopoietic progenitors. Genes Dev 25(15):1613-27. [PubMed: 21828271] [MGI Ref ID J:174416]
Heinonen KM; Vanegas JR; Brochu S; Shan J; Vainio SJ; Perreault C. 2011. Wnt4 regulates thymic cellularity through the expansion of thymic epithelial cells and early thymic progenitors. Blood 118(19):5163-73. [PubMed: 21937690] [MGI Ref ID J:178890]
Holl D; Kuckenberg P; Woynecki T; Egert A; Becker A; Huss S; Stabenow D; Zimmer A; Knolle P; Tolba R; Fischer HP; Schorle H. 2011. Transgenic Overexpression of Tcfap2c/AP-2gamma Results in Liver Failure and Intestinal Dysplasia. PLoS One 6(7):e22034. [PubMed: 21779369] [MGI Ref ID J:174049]
Jaako P; Flygare J; Olsson K; Quere R; Ehinger M; Henson A; Ellis S; Schambach A; Baum C; Richter J; Larsson J; Bryder D; Karlsson S. 2011. Mice with ribosomal protein S19 deficiency develop bone marrow failure and symptoms like patients with Diamond-Blackfan anemia. Blood 118(23):6087-96. [PubMed: 21989989] [MGI Ref ID J:179085]
Kharas MG; Lengner CJ; Al-Shahrour F; Bullinger L; Ball B; Zaidi S; Morgan K; Tam W; Paktinat M; Okabe R; Gozo M; Einhorn W; Lane SW; Scholl C; Frohling S; Fleming M; Ebert BL; Gilliland DG; Jaenisch R; Daley GQ. 2010. Musashi-2 regulates normal hematopoiesis and promotes aggressive myeloid leukemia. Nat Med 16(8):903-8. [PubMed: 20616797] [MGI Ref ID J:163322]
Lopez ME; Klein AD; Dimbil UJ; Scott MP. 2011. Anatomically defined neuron-based rescue of neurodegenerative niemann-pick type C disorder. J Neurosci 31(12):4367-78. [PubMed: 21430138] [MGI Ref ID J:170312]
Magnusson M; Brun AC; Miyake N; Larsson J; Ehinger M; Bjornsson JM; Wutz A; Sigvardsson M; Karlsson S. 2007. HOXA10 is a critical regulator for hematopoietic stem cells and erythroid/megakaryocyte development. Blood 109(9):3687-96. [PubMed: 17234739] [MGI Ref ID J:145322]
Markoulaki S; Hanna J; Beard C; Carey BW; Cheng AW; Lengner CJ; Dausman JA; Fu D; Gao Q; Wu S; Cassady JP; Jaenisch R. 2009. Transgenic mice with defined combinations of drug-inducible reprogramming factors. Nat Biotechnol 27(2):169-71. [PubMed: 19151700] [MGI Ref ID J:158492]
McJunkin K; Mazurek A; Premsrirut PK; Zuber J; Dow LE; Simon J; Stillman B; Lowe SW. 2011. Reversible suppression of an essential gene in adult mice using transgenic RNA interference. Proc Natl Acad Sci U S A 108(17):7113-8. [PubMed: 21482754] [MGI Ref ID J:171348]
Premsrirut PK; Dow LE; Kim SY; Camiolo M; Malone CD; Miething C; Scuoppo C; Zuber J; Dickins RA; Kogan SC; Shroyer KR; Sordella R; Hannon GJ; Lowe SW. 2011. A rapid and scalable system for studying gene function in mice using conditional RNA interference. Cell 145(1):145-58. [PubMed: 21458673] [MGI Ref ID J:171191]
Quere R; Andradottir S; Brun AC; Zubarev RA; Karlsson G; Olsson K; Magnusson M; Cammenga J; Karlsson S. 2011. High levels of the adhesion molecule CD44 on leukemic cells generate acute myeloid leukemia relapse after withdrawal of the initial transforming event. Leukemia 25(3):515-26. [PubMed: 21116281] [MGI Ref ID J:170242]
Stadtfeld M; Maherali N; Borkent M; Hochedlinger K. 2010. A reprogrammable mouse strain from gene-targeted embryonic stem cells. Nat Methods 7(1):53-5. [PubMed: 20010832] [MGI Ref ID J:159350]
Watanabe S; Hirai H; Asakura Y; Tastad C; Verma M; Keller C; Dutton JR; Asakura A. 2011. MyoD gene suppression by Oct4 is required for reprogramming in myoblasts to produce induced pluripotent stem cells. Stem Cells 29(3):505-16. [PubMed: 21425413] [MGI Ref ID J:175957]
Yamada Y; Aoki H; Kunisada T; Hara A. 2010. Rest promotes the early differentiation of mouse ESCs but is not required for their maintenance. Cell Stem Cell 6(1):10-5. [PubMed: 20085738] [MGI Ref ID J:157076]
Zhu H; Shah S; Shyh-Chang N; Shinoda G; Einhorn WS; Viswanathan SR; Takeuchi A; Grasemann C; Rinn JL; Lopez MF; Hirschhorn JN; Palmert MR; Daley GQ. 2010. Lin28a transgenic mice manifest size and puberty phenotypes identified in human genetic association studies. Nat Genet 42(7):626-30. [PubMed: 20512147] [MGI Ref ID J:166568]
Zhu H; Shyh-Chang N; Segre AV; Shinoda G; Shah SP; Einhorn WS; Takeuchi A; Engreitz JM; Hagan JP; Kharas MG; Urbach A; Thornton JE; Triboulet R; Gregory RI; Altshuler D; Daley GQ. 2011. The Lin28/let-7 Axis Regulates Glucose Metabolism. Cell 147(1):81-94. [PubMed: 21962509] [MGI Ref ID J:177113]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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 |
Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $261.00 Female or Male Homozygous for Gt(ROSA)26Sortm1(rtTA*M2)Jae, Heterozygous for Col1a1tm2(tetO-Pou5f1)Jae $320.00 Female or Male Homozygous for Gt(ROSA)26Sortm1(rtTA*M2)Jae, Homozygous for Col1a1tm2(tetO-Pou5f1)Jae
Pairs /Price (US dollars $) Pair Genotype $522.00 Homozygous for Gt(ROSA)26Sortm1(rtTA*M2)Jae, Heterozygous for Col1a1tm2(tetO-Pou5f1)Jae x Homozygous for Gt(ROSA)26Sortm1(rtTA*M2)Jae, Heterozygous for Col1a1tm2(tetO-Pou5f1)Jae Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $339.30 Female or Male Homozygous for Gt(ROSA)26Sortm1(rtTA*M2)Jae, Heterozygous for Col1a1tm2(tetO-Pou5f1)Jae $416.00 Female or Male Homozygous for Gt(ROSA)26Sortm1(rtTA*M2)Jae, Homozygous for Col1a1tm2(tetO-Pou5f1)Jae
Pairs /Price (US dollars $) Pair Genotype $678.60 Homozygous for Gt(ROSA)26Sortm1(rtTA*M2)Jae, Heterozygous for Col1a1tm2(tetO-Pou5f1)Jae x Homozygous for Gt(ROSA)26Sortm1(rtTA*M2)Jae, Heterozygous for Col1a1tm2(tetO-Pou5f1)Jae Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
Control Information
| Control | ||
|---|---|---|
| 101043 B6129SF1/J | (approximate) | |
| 101045 B6129SF2/J | (approximate) | |
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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