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| Within the first year 40% of 2D2 TCR mice develop spontaneous, isolated optic neuritis. Injection of suboptimal doses of myelin oligodendrocyte glycoprotein is sufficient to trigger the optic neuritis without stimulating widespread experimental autoimmune encephalomyelitis in the central nervous system. Because the spontaneous diseases observed in 2D2 TCR mice are similar in their incidence and manifestations to those developed by multiple sclerosis (MS) patients, these mice may be useful in immunological studies to investigate the role and nature of the myelin oligodendrocyte glycoprotein specific, self-reactive T cell repertoire, as well as to study optic neuritis and its relationship to the development of autoimmunity in the CNS. | |||||||||||||||||||
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Description
Strain Information
Type Coisogenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System +/+ sibling x Hemizygote (Female x Male) 17-MAY-08 Species laboratory mouse Generation N?+N3F9n1f1 (16-OCT-12)
Generation DefinitionsDonating Investigator Vijay Kuchroo, Brigham and Women's Hospital Description
Mice hemizygous for this "2D2 TCR" (or MOG 35-55 specific TCR) transgene are viable and fertile. The myelin oligodendrocyte glycoprotein (MOG)-specific transgenic T cells are not deleted nor tolerized and are functionally competent. The majority of thymocytes in 2D2 TCR mice express high and intermediate levels of the transgenic T cell receptor (TCR), indicating efficient positive selection of transgenic T cells. The majority of CD4+ splenocytes express the transgenic TCR (as defined by Valpha3.2 and Vbeta11 expression). Cultured splenocytes are responsive to whole myelin oligodendrocyte glycoprotein (MOG) and to MOG 35-55 peptide, but not to ovalbumin (OVA) control peptides. From between 2.5 to 5 months of age, 4% of 2D2 TCR mice develop spontaneous experimental autoimmune encephalomyelitis (EAE), while within the first year 40% of 2D2 TCR mice develop spontaneous, isolated optic neuritis with neither clinical nor histological evidence of EAE. Standard EAE induction protocols produce typical EAE in 2D2 TCR mice, but injection of suboptimal doses of MOG is sufficient to trigger the optic neuritis without stimulating widespread EAE in the central nervous system (CNS). Because the spontaneous diseases observed in 2D2 TCR mice are similar in their incidence and manifestations to those developed by multiple sclerosis (MS) patients, these mice may be useful in immunological studies to investigate the role and nature of the MOG-specific, self-reactive T cell repertoire, as well as to study optic neuritis and its relationship to the development of autoimmunity in the CNS.Development
A myelin oligodendrocyte glycoprotein (MOG) peptide (amino acids 35-55; MEVGWYRSPFSRVVHLYRNGK) was injected into C57BL/6 mice. T lymphocytes from lymph nodes of these immunized mice were isolated and cells expressing the T cell receptor (TCR) combination (Valpha3.2 and Vbeta11) were selected (clone 2D2). The Valpha3.2-Jalpha18 and Vbeta11-DJbeta1.1 sequences of 2D2 TCR were cloned into TCR expression cassettes and the resulting constructs were injected into the pronuclei of fertilized C57BL/6 oocytes. Founder mice were bred with C57BL/6J mice. These 2D2 TCR transgenic mice were bred with wildtype C57BL/6 for many generations prior to arrival at The Jackson Laboratory.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Tcra
View Strains carrying other alleles of Tcra (45 strains)
Phenotype
Phenotype Information
assigned by genotype
Tg(Tcra2D2,Tcrb2D2)1Kuch/0
involves: C57BL/6
- mortality/aging
- increased sensitivity to induced morbidity/mortality
- 40% of transgenic mice succumb to EAE compared to no non-transgenic littermates (MGI Ref ID J:83278)
- nervous system phenotype
- CNS inflammation
- 7/15 mice without EAE show typical myelinating/demyelinating lesions of optic neuritis (MGI Ref ID J:83278)
- mice with EAE show typical myelinating/demyelinating lesions of optic neuritis (MGI Ref ID J:83278)
- 55 and 78% of transgenic mice immunized with 100 and 10 ug of MOG 35-55 without PT, respectively, develop optic nerve lesions (MGI Ref ID J:83278)
- abnormal optic nerve morphology
- mice with or without EAE that display optic neuritis have myelinating/demyelinating lesions consisting of subpial and endoneurial mononuclear cell infiltrates with demyelination indicated by presence of foamy macrophages (MGI Ref ID J:83278)
- axon degeneration
- mice with optic neuritis have varying degrees of axonal injury and loss (MGI Ref ID J:83278)
- demyelination
- CNS tissues show myelin loss (MGI Ref ID J:83278)
- vision/eye phenotype
- abnormal eye morphology
- abnormal optic nerve morphology
- mice with or without EAE that display optic neuritis have myelinating/demyelinating lesions consisting of subpial and endoneurial mononuclear cell infiltrates with demyelination indicated by presence of foamy macrophages (MGI Ref ID J:83278)
- blepharitis
- mice without EAE develop superficial inflammation around the eyelids; this is unilateral and not observed in wild-type littermates during up to 1 year observation (MGI Ref ID J:83278)
- eyelid edema
- mice without EAE show eyelid inflammation and eyelid swelling; this is unilateral and not observed in wild-type littermates during up to 1 year observation (MGI Ref ID J:83278)
- immune system phenotype
- CNS inflammation
- 7/15 mice without EAE show typical myelinating/demyelinating lesions of optic neuritis (MGI Ref ID J:83278)
- mice with EAE show typical myelinating/demyelinating lesions of optic neuritis (MGI Ref ID J:83278)
- 55 and 78% of transgenic mice immunized with 100 and 10 ug of MOG 35-55 without PT, respectively, develop optic nerve lesions (MGI Ref ID J:83278)
- abnormal CD4-positive T cell morphology
- abnormal cytokine secretion
- spleen cells from naive mice produce high levels of IFN gamma in response to MOG 35-55 (MGI Ref ID J:83278)
- blepharitis
- mice without EAE develop superficial inflammation around the eyelids; this is unilateral and not observed in wild-type littermates during up to 1 year observation (MGI Ref ID J:83278)
- increased susceptibility to autoimmune disorder
- 4% (3/72) of mice develop spontaneous EAE, indicated initially by a limp tail, followed by hindlimb paralysis between 2.5 and 5 months of age (MGI Ref ID J:83278)
- 55 and 78% of mice immunized with 100 and 10 ug of MOG 35-55 without PT, respectively, develop optic nerve lesions (MGI Ref ID J:83278)
- mice with disease have typical myelinating/demyelinating lesions (MGI Ref ID J:83278)
- increased susceptibility to experimental autoimmune encephalomyelitis
- transgenic mice immunized with MOG 35-55 + pertussis toxin (PT) develop more severe EAE than non-transgenic littermates, with earlier onset and greater clinical scores; 50% of mice develop associated optic neuritis also (MGI Ref ID J:83278)
- injection of PT alone induces clinical EAE in 39% and histological EAE in 56% of transgenics compared to no non-transgenic mice; 80% of mice develop associated optic neuritis also (MGI Ref ID J:83278)
- hematopoietic system phenotype
- abnormal CD4-positive T cell morphology
- cellular phenotype
- increased cell proliferation
- spleen cells from naive mice show increased proliferative response to myelin oligodendrocyte protein peptide 35-55 (MOG 35-55) compared to wild-type mice (MGI Ref ID J:83278)
- homeostasis/metabolism phenotype
- edema
- EAE-affected mice showed edema in the brain and spinal cord (MGI Ref ID J:83278)
- eyelid edema
- mice without EAE show eyelid inflammation and eyelid swelling; this is unilateral and not observed in wild-type littermates during up to 1 year observation (MGI Ref ID J:83278)
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Tg(Tcra2D2,Tcrb2D2)1Kuch/?
involves: C57BL/6 * CD-1
- immune system phenotype
- decreased interleukin-17 secretion
- T cells incubated with LPS-stimulated CD11c+ dendritic cells with IL-23 in the presence of MOG peptide and neutralizing antibodies against IFN-gamma and IL-4 plus either IL-25 or IL-13 antibodies produce less IL-17 than with both IL-25 and IL-13 antibodies or without either (MGI Ref ID J:125296)
This mouse can be used to support research in many areas including:
Immunology, Inflammation and Autoimmunity Research
Autoimmunity
experimental allergic encephalomyelitis (EAE)
Immunodeficiency
multiple immune defects
Rearranged Antigen-Specific T Cell Receptor Transgenes
Research Tools
Immunology and Inflammation Research
T Cell Receptor Transgenics
Sensorineural Research
Sensorineural Research
Eye Defects
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Tcra2D2,Tcrb2D2)1Kuch | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Vijay Kuchroo | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | 2D2; 2D2 TCR; MOG TCR; TCRMOG; TCRMOG; | ||
| Mutation Made By | Vijay Kuchroo, Brigham and Women's Hospital | ||
| Strain of Origin | C57BL/6 | ||
| Expressed Gene | Tcra, T cell receptor alpha chain, mouse, laboratory | ||
| Expressed Gene | Tcrb, T cell receptor beta chain, mouse, laboratory | ||
| Promoter | Tcra, T cell receptor alpha chain, mouse, laboratory | ||
| Promoter | Tcrb, T cell receptor beta chain, mouse, laboratory | ||
| Molecular Note | The Valpha3.2Jalpha18 region of Tcra from the 2D2 T cell clone as well as the Vbeta11DJbeta1,1 region of Tcrb from the same 2D2 clone were inserted into TCR expression cassettes. The linearized plasmids were injected into the pronuclei of fertilized C57BL/6 oocytes. The 2D2 clone was purified from C57BL/6 mice immunized with a peptide from myelin oligodendrocyte glycoprotein (MOG). The amino acid sequence of the CDR3 region from TCR alpha is VYF CALRSY NFG and that from TCR beta is CASS LDCG ANP. [MGI Ref ID J:83278] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tcra2d2, Melt Curve Analysis
Tg(Tcra2D2,Tcrb2D2)1Kuch, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Bettelli E; Pagany M; Weiner HL; Linington C; Sobel RA; Kuchroo VK. 2003. Myelin oligodendrocyte glycoprotein-specific T cell receptor transgenic mice develop spontaneous autoimmune optic neuritis. J Exp Med 197(9):1073-81. [PubMed: 12732654] [MGI Ref ID J:83278]
Additional References
Tg(Tcra2D2,Tcrb2D2)1Kuch relatedAnderson AC; Lord GM; Dardalhon V; Lee DH; Sabatos-Peyton CA; Glimcher LH; Kuchroo VK. 2010. T-bet, a Th1 transcription factor regulates the expression of Tim-3. Eur J Immunol 40(3):859-66. [PubMed: 20049876] [MGI Ref ID J:157872]
Awasthi A; Carrier Y; Peron JP; Bettelli E; Kamanaka M; Flavell RA; Kuchroo VK; Oukka M; Weiner HL. 2007. A dominant function for interleukin 27 in generating interleukin 10-producing anti-inflammatory T cells. Nat Immunol 8(12):1380-9. [PubMed: 17994022] [MGI Ref ID J:127774]
Bandukwala HS; Gagnon J; Togher S; Greenbaum JA; Lamperti ED; Parr NJ; Molesworth AM; Smithers N; Lee K; Witherington J; Tough DF; Prinjha RK; Peters B; Rao A. 2012. Selective inhibition of CD4+ T-cell cytokine production and autoimmunity by BET protein and c-Myc inhibitors. Proc Natl Acad Sci U S A 109(36):14532-7. [PubMed: 22912406] [MGI Ref ID J:189876]
Bauer M; Brakebusch C; Coisne C; Sixt M; Wekerle H; Engelhardt B; Fassler R. 2009. Beta1 integrins differentially control extravasation of inflammatory cell subsets into the CNS during autoimmunity. Proc Natl Acad Sci U S A 106(6):1920-5. [PubMed: 19179279] [MGI Ref ID J:144959]
Bendix I; Pfueller CF; Leuenberger T; Glezeva N; Siffrin V; Muller Y; Prozorovski T; Hansen W; Topphoff US; Loddenkemper C; Zipp F; Waiczies S. 2010. MAPK3 deficiency drives autoimmunity via DC arming. Eur J Immunol 40(5):1486-95. [PubMed: 20186879] [MGI Ref ID J:160957]
Benkhoucha M; Santiago-Raber ML; Schneiter G; Chofflon M; Funakoshi H; Nakamura T; Lalive PH. 2010. Hepatocyte growth factor inhibits CNS autoimmunity by inducing tolerogenic dendritic cells and CD25+Foxp3+ regulatory T cells. Proc Natl Acad Sci U S A 107(14):6424-9. [PubMed: 20332205] [MGI Ref ID J:159312]
Bettelli E; Baeten D; Jager A; Sobel RA; Kuchroo VK. 2006. Myelin oligodendrocyte glycoprotein-specific T and B cells cooperate to induce a Devic-like disease in mice. J Clin Invest 116(9):2393-402. [PubMed: 16955141] [MGI Ref ID J:114666]
Bettelli E; Sullivan B; Szabo SJ; Sobel RA; Glimcher LH; Kuchroo VK. 2004. Loss of T-bet, But Not STAT1, Prevents the Development of Experimental Autoimmune Encephalomyelitis. J Exp Med 200(1):79-87. [PubMed: 15238607] [MGI Ref ID J:91376]
Bhat R; Axtell R; Mitra A; Miranda M; Lock C; Tsien RW; Steinman L. 2010. Inhibitory role for GABA in autoimmune inflammation. Proc Natl Acad Sci U S A 107(6):2580-5. [PubMed: 20133656] [MGI Ref ID J:157565]
Brandl C; Ortler S; Herrmann T; Cardell S; Lutz MB; Wiendl H. 2010. B7-H1-deficiency enhances the potential of tolerogenic dendritic cells by activating CD1d-restricted type II NKT cells. PLoS One 5(5):e10800. [PubMed: 20520738] [MGI Ref ID J:160903]
Carl JW Jr; Liu JQ; Joshi PS; El-Omrani HY; Yin L; Zheng X; Whitacre CC; Liu Y; Bai XF. 2008. Autoreactive T cells escape clonal deletion in the thymus by a CD24-dependent pathway. J Immunol 181(1):320-8. [PubMed: 18566397] [MGI Ref ID J:137407]
Carrier Y; Yuan J; Kuchroo VK; Weiner HL. 2007. Th3 cells in peripheral tolerance. I. Induction of Foxp3-positive regulatory T cells by Th3 cells derived from TGF-beta T cell-transgenic mice. J Immunol 178(1):179-85. [PubMed: 17182553] [MGI Ref ID J:141941]
Cervantes-Barragan L; Firner S; Bechmann I; Waisman A; Lahl K; Sparwasser T; Thiel V; Ludewig B. 2012. Regulatory T cells selectively preserve immune privilege of self-antigens during viral central nervous system infection. J Immunol 188(8):3678-85. [PubMed: 22407917] [MGI Ref ID J:184079]
Chen ML; Yan BS; Kozoriz D; Weiner HL. 2009. Novel CD8+ Treg suppress EAE by TGF-beta- and IFN-gamma-dependent mechanisms. Eur J Immunol 39(12):3423-35. [PubMed: 19768696] [MGI Ref ID J:155492]
Divekar RD; Haymaker CL; Cascio JA; Guloglu BF; Ellis JS; Tartar DM; Hoeman CM; Franklin CL; Zinselmeyer BH; Lynch JN; Miller MJ; Zaghouani H. 2011. T cell dynamics during induction of tolerance and suppression of experimental allergic encephalomyelitis. J Immunol 187(8):3979-86. [PubMed: 21911603] [MGI Ref ID J:179314]
Elyaman W; Bradshaw EM; Wang Y; Oukka M; Kivisakk P; Chiba S; Yagita H; Khoury SJ. 2007. JAGGED1 and delta1 differentially regulate the outcome of experimental autoimmune encephalomyelitis. J Immunol 179(9):5990-8. [PubMed: 17947672] [MGI Ref ID J:153001]
Elyaman W; Kivisakk P; Reddy J; Chitnis T; Raddassi K; Imitola J; Bradshaw E; Kuchroo VK; Yagita H; Sayegh MH; Khoury SJ. 2008. Distinct functions of autoreactive memory and effector CD4+ T cells in experimental autoimmune encephalomyelitis. Am J Pathol 173(2):411-22. [PubMed: 18583313] [MGI Ref ID J:138298]
Esplugues E; Huber S; Gagliani N; Hauser AE; Town T; Wan YY; O'Connor W Jr; Rongvaux A; Van Rooijen N; Haberman AM; Iwakura Y; Kuchroo VK; Kolls JK; Bluestone JA; Herold KC; Flavell RA. 2011. Control of TH17 cells occurs in the small intestine. Nature 475(7357):514-8. [PubMed: 21765430] [MGI Ref ID J:174687]
Francisco LM; Salinas VH; Brown KE; Vanguri VK; Freeman GJ; Kuchroo VK; Sharpe AH. 2009. PD-L1 regulates the development, maintenance, and function of induced regulatory T cells. J Exp Med 206(13):3015-29. [PubMed: 20008522] [MGI Ref ID J:155672]
Frommer F; Heinen TJ; Wunderlich FT; Yogev N; Buch T; Roers A; Bettelli E; Muller W; Anderton SM; Waisman A. 2008. Tolerance without clonal expansion: self-antigen-expressing B cells program self-reactive T cells for future deletion. J Immunol 181(8):5748-59. [PubMed: 18832734] [MGI Ref ID J:140751]
Frommer F; Waisman A. 2010. B cells participate in thymic negative selection of murine auto-reactive CD4+ T cells. PLoS One 5(10):e15372. [PubMed: 20976010] [MGI Ref ID J:166225]
Ghoreschi K; Laurence A; Yang XP; Tato CM; McGeachy MJ; Konkel JE; Ramos HL; Wei L; Davidson TS; Bouladoux N; Grainger JR; Chen Q; Kanno Y; Watford WT; Sun HW; Eberl G; Shevach EM; Belkaid Y; Cua DJ; Chen W; O'Shea JJ. 2010. Generation of pathogenic T(H)17 cells in the absence of TGF-beta signalling. Nature 467(7318):967-71. [PubMed: 20962846] [MGI Ref ID J:165551]
Glatigny S; Duhen R; Oukka M; Bettelli E. 2011. Cutting edge: loss of alpha4 integrin expression differentially affects the homing of Th1 and Th17 cells. J Immunol 187(12):6176-9. [PubMed: 22084440] [MGI Ref ID J:180388]
Gocke AR; Lebson LA; Grishkan IV; Hu L; Nguyen HM; Whartenby KA; Chandy KG; Calabresi PA. 2012. Kv1.3 deletion biases T cells toward an immunoregulatory phenotype and renders mice resistant to autoimmune encephalomyelitis. J Immunol 188(12):5877-86. [PubMed: 22581856] [MGI Ref ID J:188877]
Gong Y; Wang Z; Liang Z; Duan H; Ouyang L; Yu Q; Xu Z; Shen G; Weng X; Wu X. 2012. Soluble MOG35-55/I-A(b) dimers ameliorate experimental autoimmune encephalomyelitis by reducing encephalitogenic T cells. PLoS One 7(10):e47435. [PubMed: 23077616] [MGI Ref ID J:192210]
Greter M; Hofmann J; Becher B. 2009. Neo-lymphoid aggregates in the adult liver can initiate potent cell-mediated immunity. PLoS Biol 7(5):e1000109. [PubMed: 19468301] [MGI Ref ID J:150671]
Greve B; Weissert R; Hamdi N; Bettelli E; Sobel RA; Coyle A; Kuchroo VK; Rajewsky K; Schmidt-Supprian M. 2007. I kappa B kinase 2/beta deficiency controls expansion of autoreactive T cells and suppresses experimental autoimmune encephalomyelitis. J Immunol 179(1):179-85. [PubMed: 17579036] [MGI Ref ID J:127765]
Gris D; Ye Z; Iocca HA; Wen H; Craven RR; Gris P; Huang M; Schneider M; Miller SD; Ting JP. 2010. NLRP3 plays a critical role in the development of experimental autoimmune encephalomyelitis by mediating Th1 and Th17 responses. J Immunol 185(2):974-81. [PubMed: 20574004] [MGI Ref ID J:162018]
Guan Y; Shindler KS; Tabuena P; Rostami AM. 2006. Retinal ganglion cell damage induced by spontaneous autoimmune optic neuritis in MOG-specific TCR transgenic mice. J Neuroimmunol 178(1-2):40-8. [PubMed: 16828169] [MGI Ref ID J:127769]
Hirahara K; Ghoreschi K; Yang XP; Takahashi H; Laurence A; Vahedi G; Sciume G; Hall AO; Dupont CD; Francisco LM; Chen Q; Tanaka M; Kanno Y; Sun HW; Sharpe AH; Hunter CA; O'Shea JJ. 2012. Interleukin-27 priming of T cells controls IL-17 production in trans via induction of the ligand PD-L1. Immunity 36(6):1017-30. [PubMed: 22726954] [MGI Ref ID J:187415]
Hirota K; Duarte JH; Veldhoen M; Hornsby E; Li Y; Cua DJ; Ahlfors H; Wilhelm C; Tolaini M; Menzel U; Garefalaki A; Potocnik AJ; Stockinger B. 2011. Fate mapping of IL-17-producing T cells in inflammatory responses. Nat Immunol 12(3):255-63. [PubMed: 21278737] [MGI Ref ID J:169305]
Hochweller K; Wabnitz GH; Samstag Y; Suffner J; Hammerling GJ; Garbi N. 2010. Dendritic cells control T cell tonic signaling required for responsiveness to foreign antigen. Proc Natl Acad Sci U S A 107(13):5931-6. [PubMed: 20231464] [MGI Ref ID J:158921]
Hofmann J; Mair F; Greter M; Schmidt-Supprian M; Becher B. 2011. NIK signaling in dendritic cells but not in T cells is required for the development of effector T cells and cell-mediated immune responses. J Exp Med 208(9):1917-29. [PubMed: 21807870] [MGI Ref ID J:177597]
Huang G; Wang Y; Shi LZ; Kanneganti TD; Chi H. 2011. Signaling by the Phosphatase MKP-1 in Dendritic Cells Imprints Distinct Effector and Regulatory T Cell Fates. Immunity 35(1):45-58. [PubMed: 21723158] [MGI Ref ID J:174378]
Huang G; Wang Y; Vogel P; Kanneganti TD; Otsu K; Chi H. 2012. Signaling via the kinase p38alpha programs dendritic cells to drive TH17 differentiation and autoimmune inflammation. Nat Immunol 13(2):152-61. [PubMed: 22231518] [MGI Ref ID J:181211]
Huber M; Heink S; Pagenstecher A; Reinhard K; Ritter J; Visekruna A; Guralnik A; Bollig N; Jeltsch K; Heinemann C; Wittmann E; Buch T; Prazeres da Costa O; Brustle A; Brenner D; Mak TW; Mittrucker HW; Tackenberg B; Kamradt T; Lohoff M. 2013. IL-17A secretion by CD8+ T cells supports Th17-mediated autoimmune encephalomyelitis. J Clin Invest 123(1):247-60. [PubMed: 23221338] [MGI Ref ID J:194160]
Idoyaga J; Fiorese C; Zbytnuik L; Lubkin A; Miller J; Malissen B; Mucida D; Merad M; Steinman RM. 2013. Specialized role of migratory dendritic cells in peripheral tolerance induction. J Clin Invest :. [PubMed: 23298832] [MGI Ref ID J:194499]
Irla M; Kupfer N; Suter T; Lissilaa R; Benkhoucha M; Skupsky J; Lalive PH; Fontana A; Reith W; Hugues S. 2010. MHC class II-restricted antigen presentation by plasmacytoid dendritic cells inhibits T cell-mediated autoimmunity. J Exp Med 207(9):1891-905. [PubMed: 20696698] [MGI Ref ID J:165740]
Joller N; Hafler JP; Brynedal B; Kassam N; Spoerl S; Levin SD; Sharpe AH; Kuchroo VK. 2011. Cutting edge: TIGIT has T cell-intrinsic inhibitory functions. J Immunol 186(3):1338-42. [PubMed: 21199897] [MGI Ref ID J:168902]
King IL; Kroenke MA; Segal BM. 2010. GM-CSF-dependent, CD103+ dermal dendritic cells play a critical role in Th effector cell differentiation after subcutaneous immunization. J Exp Med 207(5):953-61. [PubMed: 20421390] [MGI Ref ID J:160936]
Kleinschek MA; Owyang AM; Joyce-Shaikh B; Langrish CL; Chen Y; Gorman DM; Blumenschein WM; McClanahan T; Brombacher F; Hurst SD; Kastelein RA; Cua DJ. 2007. IL-25 regulates Th17 function in autoimmune inflammation. J Exp Med 204(1):161-70. [PubMed: 17200411] [MGI Ref ID J:125296]
Kleinschnitz C; Schwab N; Kraft P; Hagedorn I; Dreykluft A; Schwarz T; Austinat M; Nieswandt B; Wiendl H; Stoll G. 2010. Early detrimental T-cell effects in experimental cerebral ischemia are neither related to adaptive immunity nor thrombus formation. Blood 115(18):3835-42. [PubMed: 20215643] [MGI Ref ID J:160239]
Konkel JE; Frommer F; Leech MD; Yagita H; Waisman A; Anderton SM. 2010. PD-1 signalling in CD4(+) T cells restrains their clonal expansion to an immunogenic stimulus, but is not critically required for peptide-induced tolerance. Immunology 130(1):92-102. [PubMed: 20113370] [MGI Ref ID J:166414]
Korn T; Reddy J; Gao W; Bettelli E; Awasthi A; Petersen TR; Backstrom BT; Sobel RA; Wucherpfennig KW; Strom TB; Oukka M; Kuchroo VK. 2007. Myelin-specific regulatory T cells accumulate in the CNS but fail to control autoimmune inflammation. Nat Med 13(4):423-31. [PubMed: 17384649] [MGI Ref ID J:127766]
Kreymborg K; Etzensperger R; Dumoutier L; Haak S; Rebollo A; Buch T; Heppner FL; Renauld JC; Becher B. 2007. IL-22 is expressed by Th17 cells in an IL-23-dependent fashion, but not required for the development of autoimmune encephalomyelitis. J Immunol 179(12):8098-104. [PubMed: 18056351] [MGI Ref ID J:155038]
Krishnamoorthy G; Lassmann H; Wekerle H; Holz A. 2006. Spontaneous opticospinal encephalomyelitis in a double-transgenic mouse model of autoimmune T cell/B cell cooperation. J Clin Invest 116(9):2385-92. [PubMed: 16955140] [MGI Ref ID J:114667]
Krishnamoorthy G; Saxena A; Mars LT; Domingues HS; Mentele R; Ben-Nun A; Lassmann H; Dornmair K; Kurschus FC; Liblau RS; Wekerle H. 2009. Myelin-specific T cells also recognize neuronal autoantigen in a transgenic mouse model of multiple sclerosis. Nat Med 15(6):626-32. [PubMed: 19483694] [MGI Ref ID J:151335]
Kurotaki D; Kon S; Bae K; Ito K; Matsui Y; Nakayama Y; Kanayama M; Kimura C; Narita Y; Nishimura T; Iwabuchi K; Mack M; van Rooijen N; Sakaguchi S; Uede T; Morimoto J. 2011. CSF-1-dependent red pulp macrophages regulate CD4 T cell responses. J Immunol 186(4):2229-37. [PubMed: 21239712] [MGI Ref ID J:169161]
Kurschus FC; Croxford AL; Heinen AP; Wortge S; Ielo D; Waisman A. 2010. Genetic proof for the transient nature of the Th17 phenotype. Eur J Immunol 40(12):3336-46. [PubMed: 21110317] [MGI Ref ID J:174664]
Lanz TV; Ding Z; Ho PP; Luo J; Agrawal AN; Srinagesh H; Axtell R; Zhang H; Platten M; Wyss-Coray T; Steinman L. 2010. Angiotensin II sustains brain inflammation in mice via TGF-beta. J Clin Invest 120(8):2782-94. [PubMed: 20628203] [MGI Ref ID J:163770]
Laouar Y; Town T; Jeng D; Tran E; Wan Y; Kuchroo VK; Flavell RA. 2008. TGF-beta signaling in dendritic cells is a prerequisite for the control of autoimmune encephalomyelitis. Proc Natl Acad Sci U S A 105(31):10865-70. [PubMed: 18669656] [MGI Ref ID J:140054]
Li L; Kim J; Boussiotis VA. 2010. IL-1beta-mediated signals preferentially drive conversion of regulatory T cells but not conventional T cells into IL-17-producing cells. J Immunol 185(7):4148-53. [PubMed: 20817874] [MGI Ref ID J:164301]
Liao S; Cheng G; Conner DA; Huang Y; Kucherlapati RS; Munn LL; Ruddle NH; Jain RK; Fukumura D; Padera TP. 2011. Impaired lymphatic contraction associated with immunosuppression. Proc Natl Acad Sci U S A 108(46):18784-9. [PubMed: 22065738] [MGI Ref ID J:180179]
Linker RA; Lee DH; Demir S; Wiese S; Kruse N; Siglienti I; Gerhardt E; Neumann H; Sendtner M; Luhder F; Gold R. 2010. Functional role of brain-derived neurotrophic factor in neuroprotective autoimmunity: therapeutic implications in a model of multiple sclerosis. Brain 133(Pt 8):2248-63. [PubMed: 20826430] [MGI Ref ID J:181017]
Liu JQ; Carl JW Jr; Joshi PS; RayChaudhury A; Pu XA; Shi FD; Bai XF. 2007. CD24 on the resident cells of the central nervous system enhances experimental autoimmune encephalomyelitis. J Immunol 178(10):6227-35. [PubMed: 17475850] [MGI Ref ID J:146122]
Liu JQ; Liu Z; Zhang X; Shi Y; Talebian F; Carl JW Jr; Yu C; Shi FD; Whitacre CC; Trgovcich J; Bai XF. 2012. Increased Th17 and regulatory T cell responses in EBV-induced gene 3-deficient mice lead to marginally enhanced development of autoimmune encephalomyelitis. J Immunol 188(7):3099-106. [PubMed: 22387555] [MGI Ref ID J:183091]
Liu SM; Sutherland AP; Zhang Z; Rainbow DB; Quintana FJ; Paterson AM; Sharpe AH; Oukka M; Wicker LS; Kuchroo VK. 2012. Overexpression of the Ctla-4 isoform lacking exons 2 and 3 causes autoimmunity. J Immunol 188(1):155-62. [PubMed: 22124121] [MGI Ref ID J:180821]
Liu X; Leung S; Wang C; Tan Z; Wang J; Guo TB; Fang L; Zhao Y; Wan B; Qin X; Lu L; Li R; Pan H; Song M; Liu A; Hong J; Lu H; Zhang JZ. 2010. Crucial role of interleukin-7 in T helper type 17 survival and expansion in autoimmune disease. Nat Med 16(2):191-7. [PubMed: 20062065] [MGI Ref ID J:157081]
Lu L; Ikizawa K; Hu D; Werneck MB; Wucherpfennig KW; Cantor H. 2007. Regulation of activated CD4+ T cells by NK cells via the Qa-1-NKG2A inhibitory pathway. Immunity 26(5):593-604. [PubMed: 17509909] [MGI Ref ID J:123555]
Lu L; Kim HJ; Werneck MB; Cantor H. 2008. Regulation of CD8+ regulatory T cells: Interruption of the NKG2A-Qa-1 interaction allows robust suppressive activity and resolution of autoimmune disease. Proc Natl Acad Sci U S A 105(49):19420-5. [PubMed: 19047627] [MGI Ref ID J:142087]
Mars LT; Araujo L; Kerschen P; Diem S; Bourgeois E; Van LP; Carrie N; Dy M; Liblau RS; Herbelin A. 2009. Invariant NKT cells inhibit development of the Th17 lineage. Proc Natl Acad Sci U S A 106(15):6238-43. [PubMed: 19325124] [MGI Ref ID J:147761]
Matsushita T; Horikawa M; Iwata Y; Tedder TF. 2010. Regulatory B cells (b10 cells) and regulatory T cells have independent roles in controlling experimental autoimmune encephalomyelitis initiation and late-phase immunopathogenesis. J Immunol 185(4):2240-52. [PubMed: 20624940] [MGI Ref ID J:162387]
Melton AC; Bailey-Bucktrout SL; Travis MA; Fife BT; Bluestone JA; Sheppard D. 2010. Expression of alphavbeta8 integrin on dendritic cells regulates Th17 cell development and experimental autoimmune encephalomyelitis in mice. J Clin Invest 120(12):4436-44. [PubMed: 21099117] [MGI Ref ID J:171861]
Mills JH; Kim DG; Krenz A; Chen JF; Bynoe MS. 2012. A2A adenosine receptor signaling in lymphocytes and the central nervous system regulates inflammation during experimental autoimmune encephalomyelitis. J Immunol 188(11):5713-22. [PubMed: 22529293] [MGI Ref ID J:188748]
Murugaiyan G; Beynon V; Pires Da Cunha A; Joller N; Weiner HL. 2012. IFN-gamma limits Th9-mediated autoimmune inflammation through dendritic cell modulation of IL-27. J Immunol 189(11):5277-83. [PubMed: 23125412] [MGI Ref ID J:190977]
Murugaiyan G; Mittal A; Weiner HL. 2010. Identification of an IL-27/osteopontin axis in dendritic cells and its modulation by IFN-gamma limits IL-17-mediated autoimmune inflammation. Proc Natl Acad Sci U S A 107(25):11495-500. [PubMed: 20534530] [MGI Ref ID J:161596]
Murugaiyan G; Mittal A; Weiner HL. 2008. Increased osteopontin expression in dendritic cells amplifies IL-17 production by CD4+ T cells in experimental autoimmune encephalomyelitis and in multiple sclerosis. J Immunol 181(11):7480-8. [PubMed: 19017937] [MGI Ref ID J:142205]
Nowak EC; de Vries VC; Wasiuk A; Ahonen C; Bennett KA; Le Mercier I; Ha DG; Noelle RJ. 2012. Tryptophan hydroxylase-1 regulates immune tolerance and inflammation. J Exp Med 209(11):2127-35. [PubMed: 23008335] [MGI Ref ID J:190958]
O'Connell RM; Kahn D; Gibson WS; Round JL; Scholz RL; Chaudhuri AA; Kahn ME; Rao DS; Baltimore D. 2010. MicroRNA-155 promotes autoimmune inflammation by enhancing inflammatory T cell development. Immunity 33(4):607-19. [PubMed: 20888269] [MGI Ref ID J:165652]
Oh K; Park HB; Seo MW; Byoun OJ; Lee DS. 2012. Transglutaminase 2 exacerbates experimental autoimmune encephalomyelitis through positive regulation of encephalitogenic T cell differentiation and inflammation. Clin Immunol 145(2):122-32. [PubMed: 23001131] [MGI Ref ID J:190627]
Perona-Wright G; Jenkins SJ; O'Connor RA; Zienkiewicz D; McSorley HJ; Maizels RM; Anderton SM; MacDonald AS. 2009. A pivotal role for CD40-mediated IL-6 production by dendritic cells during IL-17 induction in vivo. J Immunol 182(5):2808-15. [PubMed: 19234175] [MGI Ref ID J:146255]
Peters A; Pitcher LA; Sullivan JM; Mitsdoerffer M; Acton SE; Franz B; Wucherpfennig K; Turley S; Carroll MC; Sobel RA; Bettelli E; Kuchroo VK. 2011. Th17 cells induce ectopic lymphoid follicles in central nervous system tissue inflammation. Immunity 35(6):986-96. [PubMed: 22177922] [MGI Ref ID J:179284]
Petersen TR; Gulland S; Bettelli E; Kuchroo V; Palmer E; Backstrom BT. 2004. A chimeric T cell receptor with super-signaling properties. Int Immunol 16(7):889-94. [PubMed: 15148288] [MGI Ref ID J:125963]
Petersen TR; Lata R; Spittle E; Backstrom BT. 2007. A chimeric TCR-beta chain confers increased susceptibility to EAE. Mol Immunol 44(14):3473-81. [PubMed: 17481734] [MGI Ref ID J:125962]
Qin H; Yeh WI; De Sarno P; Holdbrooks AT; Liu Y; Muldowney MT; Reynolds SL; Yanagisawa LL; Fox TH 3rd; Park K; Harrington LE; Raman C; Benveniste EN. 2012. Signal transducer and activator of transcription-3/suppressor of cytokine signaling-3 (STAT3/SOCS3) axis in myeloid cells regulates neuroinflammation. Proc Natl Acad Sci U S A 109(13):5004-9. [PubMed: 22411837] [MGI Ref ID J:182215]
Quintana FJ; Basso AS; Iglesias AH; Korn T; Farez MF; Bettelli E; Caccamo M; Oukka M; Weiner HL. 2008. Control of T(reg) and T(H)17 cell differentiation by the aryl hydrocarbon receptor. Nature 453(7191):65-71. [PubMed: 18362915] [MGI Ref ID J:136052]
Reboldi A; Coisne C; Baumjohann D; Benvenuto F; Bottinelli D; Lira S; Uccelli A; Lanzavecchia A; Engelhardt B; Sallusto F. 2009. C-C chemokine receptor 6-regulated entry of TH-17 cells into the CNS through the choroid plexus is required for the initiation of EAE. Nat Immunol 10(5):514-23. [PubMed: 19305396] [MGI Ref ID J:148286]
Rothhammer V; Heink S; Petermann F; Srivastava R; Claussen MC; Hemmer B; Korn T. 2011. Th17 lymphocytes traffic to the central nervous system independently of alpha4 integrin expression during EAE. J Exp Med 208(12):2465-76. [PubMed: 22025301] [MGI Ref ID J:178760]
Sayed BA; Christy AL; Walker ME; Brown MA. 2010. Meningeal mast cells affect early T cell central nervous system infiltration and blood-brain barrier integrity through TNF: a role for neutrophil recruitment? J Immunol 184(12):6891-900. [PubMed: 20488789] [MGI Ref ID J:161131]
Sestero CM; McGuire DJ; De Sarno P; Brantley EC; Soldevila G; Axtell RC; Raman C. 2012. CD5-dependent CK2 activation pathway regulates threshold for T cell anergy. J Immunol 189(6):2918-30. [PubMed: 22904299] [MGI Ref ID J:189919]
Shinohara ML; Kim JH; Garcia VA; Cantor H. 2008. Engagement of the type I interferon receptor on dendritic cells inhibits T helper 17 cell development: role of intracellular osteopontin. Immunity 29(1):68-78. [PubMed: 18619869] [MGI Ref ID J:137879]
Skarica M; Wang T; McCadden E; Kardian D; Calabresi PA; Small D; Whartenby KA. 2009. Signal transduction inhibition of APCs diminishes th17 and Th1 responses in experimental autoimmune encephalomyelitis. J Immunol 182(7):4192-9. [PubMed: 19299717] [MGI Ref ID J:147124]
Sobottka B; Harrer MD; Ziegler U; Fischer K; Wiendl H; Hunig T; Becher B; Goebels N. 2009. Collateral bystander damage by myelin-directed CD8+ T cells causes axonal loss. Am J Pathol 175(3):1160-6. [PubMed: 19700745] [MGI Ref ID J:153125]
Solomon BD; Mueller C; Chae WJ; Alabanza LM; Bynoe MS. 2011. Neuropilin-1 attenuates autoreactivity in experimental autoimmune encephalomyelitis. Proc Natl Acad Sci U S A 108(5):2040-5. [PubMed: 21245328] [MGI Ref ID J:169116]
Takahashi H; Kanno T; Nakayamada S; Hirahara K; Sciume G; Muljo SA; Kuchen S; Casellas R; Wei L; Kanno Y; O'Shea JJ. 2012. TGF-beta and retinoic acid induce the microRNA miR-10a, which targets Bcl-6 and constrains the plasticity of helper T cells. Nat Immunol 13(6):587-95. [PubMed: 22544395] [MGI Ref ID J:186450]
Tsai VW; Mohammad MG; Tolhurst O; Breit SN; Sawchenko PE; Brown DA. 2011. CCAAT/Enhancer Binding Protein-delta Expression by Dendritic Cells Regulates CNS Autoimmune Inflammatory Disease. J Neurosci 31(48):17612-21. [PubMed: 22131422] [MGI Ref ID J:178141]
Withers DR; Jaensson E; Gaspal F; McConnell FM; Eksteen B; Anderson G; Agace WW; Lane PJ. 2009. The survival of memory CD4+ T cells within the gut lamina propria requires OX40 and CD30 signals. J Immunol 183(8):5079-84. [PubMed: 19786532] [MGI Ref ID J:153824]
Yogev N; Frommer F; Lukas D; Kautz-Neu K; Karram K; Ielo D; von Stebut E; Probst HC; van den Broek M; Riethmacher D; Birnberg T; Blank T; Reizis B; Korn T; Wiendl H; Jung S; Prinz M; Kurschus FC; Waisman A. 2012. Dendritic Cells Ameliorate Autoimmunity in the CNS by Controlling the Homeostasis of PD-1 Receptor(+) Regulatory T Cells. Immunity 37(2):264-75. [PubMed: 22902234] [MGI Ref ID J:187367]
Yoshizaki A; Miyagaki T; DiLillo DJ; Matsushita T; Horikawa M; Kountikov EI; Spolski R; Poe JC; Leonard WJ; Tedder TF. 2012. Regulatory B cells control T-cell autoimmunity through IL-21-dependent cognate interactions. Nature 491(7423):264-8. [PubMed: 23064231] [MGI Ref ID J:189218]
Zhang X; Liu JQ; Shi Y; Reid HH; Boyd RL; Khattabi M; El-Omrani HY; Zheng P; Liu Y; Bai XF. 2012. CD24 on thymic APCs regulates negative selection of myelin antigen-specific T lymphocytes. Eur J Immunol 42(4):924-35. [PubMed: 22213356] [MGI Ref ID J:187788]
Zhu B; Kennedy JK; Wang Y; Sandoval-Garcia C; Cao L; Xiao S; Wu C; Elyaman W; Khoury SJ. 2011. Plasticity of Ly-6C(hi) myeloid cells in T cell regulation. J Immunol 187(5):2418-32. [PubMed: 21824867] [MGI Ref ID J:179261]
Zozulya AL; Ortler S; Fabry Z; Sandor M; Wiendl H. 2009. The level of B7 homologue 1 expression on brain DC is decisive for CD8 Treg cell recruitment into the CNS during EAE. Eur J Immunol 39(6):1536-43. [PubMed: 19424967] [MGI Ref ID J:149550]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, hemizygous mice are bred to wildtype siblings or to inbred C57BL/6J (Stock No. 000664). Mating System +/+ sibling x Hemizygote (Female x Male) 17-MAY-08 Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Female or Male Hemizygous for Tg(Tcra2D2,Tcrb2D2)1Kuch
Price per Pair (US dollars $) Pair Genotype $296.00 Hemizygous for Tg(Tcra2D2,Tcrb2D2)1Kuch x Noncarrier $296.00 Noncarrier x Hemizygous for Tg(Tcra2D2,Tcrb2D2)1Kuch Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Female or Male Hemizygous for Tg(Tcra2D2,Tcrb2D2)1Kuch
Price per Pair (US dollars $) Pair Genotype $384.80 Hemizygous for Tg(Tcra2D2,Tcrb2D2)1Kuch x Noncarrier $384.80 Noncarrier x Hemizygous for Tg(Tcra2D2,Tcrb2D2)1Kuch Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
JAX® Mice
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- Use of MICE by companies or for-profit entities requires a license prior to shipping.
Contact information
General inquiries regarding Terms of UseContracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
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In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
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In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.