Strain Name:

C57BL/6-Tg(Tcra2D2,Tcrb2D2)1Kuch/J

Stock Number:

006912

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Availability:

Repository- Live

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These 2D2 TCR transgenic mice develop spontaneous diseases similar in incidence and manifestation to those developed by multiple sclerosis patients. These mice may be useful for studying the myelin oligodendrocyte glycoprotein specific, self-reactive T cell repertoire, as well as to study optic neuritis and its relationship to the development of autoimmunity in the CNS.

Description

Strain Information

Type Coisogenic; Mutant Strain; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
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Mating System+/+ sibling x Hemizygote         (Female x Male)   17-MAY-08
Specieslaboratory mouse
GenerationN?+N5F4 (11-DEC-13)
Generation Definitions
 
Donating Investigator Vijay Kuchroo,   Brigham and Women's Hospital

Description
Mice hemizygous for this "2D2 TCR" (or MOG 35-55 specific TCR) transgene are viable and fertile. The myelin oligodendrocyte glycoprotein (MOG)-specific transgenic T cells are not deleted nor tolerized and are functionally competent. The majority of thymocytes in 2D2 TCR mice express high and intermediate levels of the transgenic T cell receptor (TCR), indicating efficient positive selection of transgenic T cells. The majority of CD4+ splenocytes express the transgenic TCR (as defined by Valpha3.2 and Vbeta11 expression). Cultured splenocytes are responsive to whole myelin oligodendrocyte glycoprotein (MOG) and to MOG 35-55 peptide, but not to ovalbumin (OVA) control peptides. From between 2.5 to 5 months of age, 4% of 2D2 TCR mice develop spontaneous experimental autoimmune encephalomyelitis (EAE), while within the first year 40% of 2D2 TCR mice develop spontaneous, isolated optic neuritis with neither clinical nor histological evidence of EAE. Standard EAE induction protocols produce typical EAE in 2D2 TCR mice, but injection of suboptimal doses of MOG is sufficient to trigger the optic neuritis without stimulating widespread EAE in the central nervous system (CNS). Because the spontaneous diseases observed in 2D2 TCR mice are similar in their incidence and manifestations to those developed by multiple sclerosis (MS) patients, these mice may be useful in immunological studies to investigate the role and nature of the MOG-specific, self-reactive T cell repertoire, as well as to study optic neuritis and its relationship to the development of autoimmunity in the CNS.

Development
A myelin oligodendrocyte glycoprotein (MOG) peptide (amino acids 35-55; MEVGWYRSPFSRVVHLYRNGK) was injected into C57BL/6 mice. T lymphocytes from lymph nodes of these immunized mice were isolated and cells expressing the T cell receptor (TCR) combination (Valpha3.2 and Vbeta11) were selected (clone 2D2). The Valpha3.2-Jalpha18 and Vbeta11-DJbeta1.1 sequences of 2D2 TCR were cloned into TCR expression cassettes and the resulting constructs were injected into the pronuclei of fertilized C57BL/6 oocytes. Founder mice were bred with C57BL/6J mice. These 2D2 TCR transgenic mice were bred with wildtype C57BL/6 for many generations prior to arrival at The Jackson Laboratory.

Control Information

  Control
   Noncarrier
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Tcra
005308   B10.Cg-H2d Tg(TcraCl4,TcrbCl4)1Shrm/ShrmJ
005895   B10.Cg-Thy1a H2d Tg(TcraCl1,TcrbCl1)1Shrm/J
002761   B10.Cg-Tg(TcrAND)53Hed/J
003147   B10.D2-Hc1 H2d H2-T18c/nSnJ-Tg(DO11.10)10Dlo/J
003199   B10.PL-H2u H2-T18a/(73NS)Sn-Tg(TCRA)B1Jg/J
002116   B6.129S2-Tcratm1Mom/J
022073   B6.Cg-Rag1tm1Mom Thy1a Tg(Tcra2C,Tcrb2C)1Dlo/J
008684   B6.Cg-Rag1tm1Mom Tyrp1B-w Tg(Tcra,Tcrb)9Rest/J
014550   B6.Cg-Thy1a Tg(TcraCWM5,TcrbCWM5)1807Wuth/J
005023   B6.Cg-Thy1a/Cy Tg(TcraTcrb)8Rest/J
005655   B6.Cg-Tg(Tcra,Tcrb)3Ayr/J
008428   B6.Cg-Tg(Tcra,Tcrb)HRCAll/J
008429   B6.Cg-Tg(Tcra,Tcrb)HRVAll/J
008006   B6.Cg-Tg(Tcra51-11.5,Tcrb51-11.5)AR206Ayr/J
004194   B6.Cg-Tg(TcraTcrb)425Cbn/J
005236   B6.Cg-Tg(TcraY1,TcrbY1)416Tev/J
004554   B6.NOD-(D17Mit21-D17Mit10) Tg(TCRaAI4)1Dvs/DvsJ
002115   B6;129S2-Tcratm1Mom/J
004694   B6;D2-Tg(TcrLCMV)327Sdz/JDvsJ
002408   B6;SJL-Tg(TcrAND)53Hed/J
007848   BXSB.129P2(Cg)-Tcratm1Mjo/TheoJ
021880   BXSB.B6-Tg(TcraTcrb)1100Mjb/DcrJ
004364   C.Cg-Tcratm1Mom Tcrbtm1Mom/J
003303   C.Cg-Tg(DO11.10)10Dlo/J
002045   C.SJL-Tcrac/SlkJ
002047   C.SJL-Tcrba Tcrac/SlkJ
014639   C57BL/6-Tg(Cd4-TcraDN32D3)1Aben/J
011005   C57BL/6-Tg(H2-Kb-Tcra,-Tcrb)P25Ktk/J
003831   C57BL/6-Tg(TcraTcrb)1100Mjb/J
005307   CBy.Cg-Thy1a Tg(TcraCl4,TcrbCl4)1Shrm/ShrmJ
005922   CBy.Cg-Thy1a Tg(TcraCl1,TcrbCl1)1Shrm/J
005694   D1Lac.Cg-Tg(Tcra,Tcrb)24Efro/J
017314   NOD-Tg(TcraTcrb)2H6Lwn/J
004444   NOD.129P2(C)-Tcratm1Mjo/DoiJ
006436   NOD.Cg-(Gpi1-D7Mit346)C57BL/6J Tg(TcraAI4)1Dvs/DvsJ
026243   NOD.Cg-(D9rs4135590-D9rs13480186)H2k2Tg(ILK3mHEL)3Ccg Tg(TcrHEL3A9)1Mmd/SlsgJ
026624   NOD.Cg-(D9rs6385855-D9rs13480186)H2k2Tg(ILK3mHEL)3Ccg Tg(TcrHEL3A9)1Mmd/SlsgJ
004257   NOD.Cg-Prkdcscid Tg(TcrLCMV)327Sdz/DvsJ
004347   NOD.Cg-Rag1tm1Mom Tg(TcraAI4)1Dvs/DvsJ
009377   NOD.Cg-Rag1tm1Mom Tg(TcraBDC12-4.1)10Jos Tg(TcrbBDC12-4.1)82Gse/J
024476   NOD.Cg-Stat4tm1Gru Thy1a Ifngr1tm1Agt Tg(TcraBDC2.5,TcrbBDC2.5)1Doi/LmbrJ
005686   NOD.Cg-Thy1a Tg(TcraCl4,TcrbCl4)1Shrm/ShrmJ
004696   NOD.Cg-Tg(TcrLCMV)327Sdz/DvsJ
004460   NOD.Cg-Tg(TcraBDC2.5,TcrbBDC2.5)1Doi/DoiJ
010526   NOD.Cg-Tg(TcraTcrbNY4.1)1Pesa/DvsJ
005868   NOD.Cg-Tg(TcraTcrbNY8.3)1Pesa/DvsJ
006303   NOD.FVB-Tg(TcraBDC12-4.1)10Jos/GseJ
004334   NOD/ShiLt-Tg(TcraAI4)1Dvs
018030   SJL.Cg-Tg(TcraTcrbVP2)1Bkim/J
002597   STOCK Tg(TcrHEL3A9)1Mmd/J
View Strains carrying other alleles of Tcra     (50 strains)

Strains carrying other alleles of Tcrb
005308   B10.Cg-H2d Tg(TcraCl4,TcrbCl4)1Shrm/ShrmJ
005895   B10.Cg-Thy1a H2d Tg(TcraCl1,TcrbCl1)1Shrm/J
002761   B10.Cg-Tg(TcrAND)53Hed/J
003147   B10.D2-Hc1 H2d H2-T18c/nSnJ-Tg(DO11.10)10Dlo/J
003200   B10.PL-H2u H2-T18a/(73NS)Sn-Tg(TCRB)C14Jg/J
002122   B6.129P2-Tcrbtm1Mom Tcrdtm1Mom/J
002118   B6.129P2-Tcrbtm1Mom/J
022073   B6.Cg-Rag1tm1Mom Thy1a Tg(Tcra2C,Tcrb2C)1Dlo/J
008684   B6.Cg-Rag1tm1Mom Tyrp1B-w Tg(Tcra,Tcrb)9Rest/J
014550   B6.Cg-Thy1a Tg(TcraCWM5,TcrbCWM5)1807Wuth/J
005023   B6.Cg-Thy1a/Cy Tg(TcraTcrb)8Rest/J
005655   B6.Cg-Tg(Tcra,Tcrb)3Ayr/J
008428   B6.Cg-Tg(Tcra,Tcrb)HRCAll/J
008429   B6.Cg-Tg(Tcra,Tcrb)HRVAll/J
008006   B6.Cg-Tg(Tcra51-11.5,Tcrb51-11.5)AR206Ayr/J
004194   B6.Cg-Tg(TcraTcrb)425Cbn/J
005236   B6.Cg-Tg(TcraY1,TcrbY1)416Tev/J
008430   B6.Cg-Tg(Tcrb)HRBAll/J
004555   B6.NOD-(D17Mit21-D17Mit10) Tg(TCRbAI4)1Dvs/DvsJ
002121   B6;129P-Tcrbtm1Mom Tcrdtm1Mom/J
002117   B6;129P2-Tcrbtm1Mom/J
004694   B6;D2-Tg(TcrLCMV)327Sdz/JDvsJ
002408   B6;SJL-Tg(TcrAND)53Hed/J
021880   BXSB.B6-Tg(TcraTcrb)1100Mjb/DcrJ
004364   C.Cg-Tcratm1Mom Tcrbtm1Mom/J
003303   C.Cg-Tg(DO11.10)10Dlo/J
002047   C.SJL-Tcrba Tcrac/SlkJ
002046   C.SJL-Tcrba/SlkJ
011005   C57BL/6-Tg(H2-Kb-Tcra,-Tcrb)P25Ktk/J
003831   C57BL/6-Tg(TcraTcrb)1100Mjb/J
003540   C57L/J-Tg(Tcrb)93Vbo/J
005307   CBy.Cg-Thy1a Tg(TcraCl4,TcrbCl4)1Shrm/ShrmJ
005922   CBy.Cg-Thy1a Tg(TcraCl1,TcrbCl1)1Shrm/J
007081   CByJ.129P2(B6)-Tcrbtm1Mom/J
005694   D1Lac.Cg-Tg(Tcra,Tcrb)24Efro/J
017314   NOD-Tg(TcraTcrb)2H6Lwn/J
023082   NOD.129P2(Cg)-Tcrbtm1Mom/MnkaJ
006437   NOD.Cg-(Gpi1-D7Mit346)C57BL/6J Tg(TcrbAI4)1Dvs/DvsJ
026243   NOD.Cg-(D9rs4135590-D9rs13480186)H2k2Tg(ILK3mHEL)3Ccg Tg(TcrHEL3A9)1Mmd/SlsgJ
026624   NOD.Cg-(D9rs6385855-D9rs13480186)H2k2Tg(ILK3mHEL)3Ccg Tg(TcrHEL3A9)1Mmd/SlsgJ
004257   NOD.Cg-Prkdcscid Tg(TcrLCMV)327Sdz/DvsJ
009377   NOD.Cg-Rag1tm1Mom Tg(TcraBDC12-4.1)10Jos Tg(TcrbBDC12-4.1)82Gse/J
024476   NOD.Cg-Stat4tm1Gru Thy1a Ifngr1tm1Agt Tg(TcraBDC2.5,TcrbBDC2.5)1Doi/LmbrJ
005686   NOD.Cg-Thy1a Tg(TcraCl4,TcrbCl4)1Shrm/ShrmJ
004696   NOD.Cg-Tg(TcrLCMV)327Sdz/DvsJ
004460   NOD.Cg-Tg(TcraBDC2.5,TcrbBDC2.5)1Doi/DoiJ
010526   NOD.Cg-Tg(TcraTcrbNY4.1)1Pesa/DvsJ
005868   NOD.Cg-Tg(TcraTcrbNY8.3)1Pesa/DvsJ
006304   NOD.FVB-Tg(TcrbBDC12-4.1)82Gse/GseJ
004335   NOD/ShiLt-Tg(TcrbAI4)1Dvs
018030   SJL.Cg-Tg(TcraTcrbVP2)1Bkim/J
002597   STOCK Tg(TcrHEL3A9)1Mmd/J
View Strains carrying other alleles of Tcrb     (52 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Tg(Tcra2D2,Tcrb2D2)1Kuch/0

        involves: C57BL/6
  • mortality/aging
  • increased sensitivity to induced morbidity/mortality
    • 40% of transgenic mice succumb to EAE compared to no non-transgenic littermates   (MGI Ref ID J:83278)
  • nervous system phenotype
  • CNS inflammation
    • 7/15 mice without EAE show typical myelinating/demyelinating lesions of optic neuritis   (MGI Ref ID J:83278)
    • mice with EAE show typical myelinating/demyelinating lesions of optic neuritis   (MGI Ref ID J:83278)
    • 55 and 78% of transgenic mice immunized with 100 and 10 ug of MOG 35-55 without PT, respectively, develop optic nerve lesions   (MGI Ref ID J:83278)
  • abnormal optic nerve morphology
    • mice with or without EAE that display optic neuritis have myelinating/demyelinating lesions consisting of subpial and endoneurial mononuclear cell infiltrates with demyelination indicated by presence of foamy macrophages   (MGI Ref ID J:83278)
  • axon degeneration
    • mice with optic neuritis have varying degrees of axonal injury and loss   (MGI Ref ID J:83278)
  • demyelination
    • CNS tissues show myelin loss   (MGI Ref ID J:83278)
  • vision/eye phenotype
  • abnormal eye morphology
    • superficial eye lesions in mice without EAE are often associated with progressive atrophy of the eye   (MGI Ref ID J:83278)
    • 67% of mutants show these eye lesions compared to no wild-type   (MGI Ref ID J:83278)
    • abnormal optic nerve morphology
      • mice with or without EAE that display optic neuritis have myelinating/demyelinating lesions consisting of subpial and endoneurial mononuclear cell infiltrates with demyelination indicated by presence of foamy macrophages   (MGI Ref ID J:83278)
    • blepharitis
      • mice without EAE develop superficial inflammation around the eyelids; this is unilateral and not observed in wild-type littermates during up to 1 year observation   (MGI Ref ID J:83278)
    • eyelid edema
      • mice without EAE show eyelid inflammation and eyelid swelling; this is unilateral and not observed in wild-type littermates during up to 1 year observation   (MGI Ref ID J:83278)
  • immune system phenotype
  • CNS inflammation
    • 7/15 mice without EAE show typical myelinating/demyelinating lesions of optic neuritis   (MGI Ref ID J:83278)
    • mice with EAE show typical myelinating/demyelinating lesions of optic neuritis   (MGI Ref ID J:83278)
    • 55 and 78% of transgenic mice immunized with 100 and 10 ug of MOG 35-55 without PT, respectively, develop optic nerve lesions   (MGI Ref ID J:83278)
  • abnormal CD4-positive, alpha beta T cell morphology
    • CD4/CD8 single positive ratio in thymus of transgenic mice is biased toward CD4+ compartment   (MGI Ref ID J:83278)
    • analysis shows a skewing toward CD4+ T cells in spleens as well   (MGI Ref ID J:83278)
  • abnormal cytokine secretion
    • spleen cells from naive mice produce high levels of IFN gamma in response to MOG 35-55   (MGI Ref ID J:83278)
  • blepharitis
    • mice without EAE develop superficial inflammation around the eyelids; this is unilateral and not observed in wild-type littermates during up to 1 year observation   (MGI Ref ID J:83278)
  • increased susceptibility to autoimmune disorder
    • 4% (3/72) of mice develop spontaneous EAE, indicated initially by a limp tail, followed by hindlimb paralysis between 2.5 and 5 months of age   (MGI Ref ID J:83278)
    • 55 and 78% of mice immunized with 100 and 10 ug of MOG 35-55 without PT, respectively, develop optic nerve lesions   (MGI Ref ID J:83278)
    • mice with disease have typical myelinating/demyelinating lesions   (MGI Ref ID J:83278)
    • increased susceptibility to experimental autoimmune encephalomyelitis
      • transgenic mice immunized with MOG 35-55 + pertussis toxin (PT) develop more severe EAE than non-transgenic littermates, with earlier onset and greater clinical scores; 50% of mice develop associated optic neuritis also   (MGI Ref ID J:83278)
      • injection of PT alone induces clinical EAE in 39% and histological EAE in 56% of transgenics compared to no non-transgenic mice; 80% of mice develop associated optic neuritis also   (MGI Ref ID J:83278)
  • hematopoietic system phenotype
  • abnormal CD4-positive, alpha beta T cell morphology
    • CD4/CD8 single positive ratio in thymus of transgenic mice is biased toward CD4+ compartment   (MGI Ref ID J:83278)
    • analysis shows a skewing toward CD4+ T cells in spleens as well   (MGI Ref ID J:83278)
  • cellular phenotype
  • increased cell proliferation
    • spleen cells from naive mice show increased proliferative response to myelin oligodendrocyte protein peptide 35-55 (MOG 35-55) compared to wild-type mice   (MGI Ref ID J:83278)
  • homeostasis/metabolism phenotype
  • edema
    • EAE-affected mice showed edema in the brain and spinal cord   (MGI Ref ID J:83278)
    • eyelid edema
      • mice without EAE show eyelid inflammation and eyelid swelling; this is unilateral and not observed in wild-type littermates during up to 1 year observation   (MGI Ref ID J:83278)

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Tg(Tcra2D2,Tcrb2D2)1Kuch/0

        involves: 129P2/OlaHsd * C57BL/6
  • immune system phenotype
  • abnormal T-helper 1 cell number
    • under nonpolarizing conditions, Th1 cells are reduced 50% compared to in wild-type but are 3-fold higher than in Cd5tm1Cgn/Cd5tm1Cgn Tg(Tcra2D2,Tcrb2D2)1Kuch mice   (MGI Ref ID J:189919)
    • in T cells cultures stimulated with MOG35-55   (MGI Ref ID J:189919)
  • decreased T cell proliferation
    • of CD4+ T cells stimulated with anti-CD3   (MGI Ref ID J:189919)
  • decreased T-helper 17 cell number
    • 50% under Th17 polarizing conditions compared with wild-type control and Cd5tm1Cgn/Cd5tm1Cgn Tg(Tcra2D2,Tcrb2D2)1Kuch mice   (MGI Ref ID J:189919)
    • in T cells cultures stimulated with MOG35-55   (MGI Ref ID J:189919)
  • decreased T-helper 2 cell number
    • under Th2 polarizing conditions compared with wild-type controls and Cd5tm1Cgn/Cd5tm1Cgn Tg(Tcra2D2,Tcrb2D2)1Kuch mice   (MGI Ref ID J:189919)
  • decreased interferon-gamma secretion
    • in CD4+ T cells under nonpolarizing conditions   (MGI Ref ID J:189919)
  • decreased interleukin-10 secretion
    • IL10 levels in T cells stimulated with MOG35-55 rise and contract unlike in cells from wild-type control and Cd5tm1Cgn/Cd5tm1Cgn Tg(Tcra2D2,Tcrb2D2)1Kuch mice where levels continue to rise   (MGI Ref ID J:189919)
  • decreased interleukin-2 secretion
    • IL2 levels from T cells stimulated with MOG35-55 do not increase beyond day 1unlike in cells from wild-type control   (MGI Ref ID J:189919)
  • increased T cell apoptosis
    • under Th17 polarizing conditions   (MGI Ref ID J:189919)
  • increased T cell proliferation
    • in CD4+ T cells stimulated with MOG35-55, rested then restimulated with MOG35-55 compared with cells from wild-type control and Cd5tm1Cgn/Cd5tm1Cgn Tg(Tcra2D2,Tcrb2D2)1Kuch mice   (MGI Ref ID J:189919)
  • increased interferon-gamma secretion
    • in CD4+ T cells stimulated with MOG35-55 compared with cells from wild-type control and Cd5tm1Cgn/Cd5tm1Cgn Tg(Tcra2D2,Tcrb2D2)1Kuch mice   (MGI Ref ID J:189919)
  • increased interleukin-17 secretion
    • in CD4+ T cells stimulated with MOG35-55 compared with cells from wild-type control and Cd5tm1Cgn/Cd5tm1Cgn Tg(Tcra2D2,Tcrb2D2)1Kuch mice   (MGI Ref ID J:189919)
  • increased interleukin-6 secretion
    • in CD4+ T cells stimulated with MOG35-55 compared with cells from wild-type control and Cd5tm1Cgn/Cd5tm1Cgn Tg(Tcra2D2,Tcrb2D2)1Kuch mice   (MGI Ref ID J:189919)
  • cellular phenotype
  • increased T cell apoptosis
    • under Th17 polarizing conditions   (MGI Ref ID J:189919)
  • hematopoietic system phenotype
  • abnormal T-helper 1 cell number
    • under nonpolarizing conditions, Th1 cells are reduced 50% compared to in wild-type but are 3-fold higher than in Cd5tm1Cgn/Cd5tm1Cgn Tg(Tcra2D2,Tcrb2D2)1Kuch mice   (MGI Ref ID J:189919)
    • in T cells cultures stimulated with MOG35-55   (MGI Ref ID J:189919)
  • decreased T cell proliferation
    • of CD4+ T cells stimulated with anti-CD3   (MGI Ref ID J:189919)
  • decreased T-helper 17 cell number
    • 50% under Th17 polarizing conditions compared with wild-type control and Cd5tm1Cgn/Cd5tm1Cgn Tg(Tcra2D2,Tcrb2D2)1Kuch mice   (MGI Ref ID J:189919)
    • in T cells cultures stimulated with MOG35-55   (MGI Ref ID J:189919)
  • decreased T-helper 2 cell number
    • under Th2 polarizing conditions compared with wild-type controls and Cd5tm1Cgn/Cd5tm1Cgn Tg(Tcra2D2,Tcrb2D2)1Kuch mice   (MGI Ref ID J:189919)
  • increased T cell apoptosis
    • under Th17 polarizing conditions   (MGI Ref ID J:189919)
  • increased T cell proliferation
    • in CD4+ T cells stimulated with MOG35-55, rested then restimulated with MOG35-55 compared with cells from wild-type control and Cd5tm1Cgn/Cd5tm1Cgn Tg(Tcra2D2,Tcrb2D2)1Kuch mice   (MGI Ref ID J:189919)

Tg(Tcra2D2,Tcrb2D2)1Kuch/?

        involves: C57BL/6 * CD-1
  • immune system phenotype
  • decreased interleukin-17 secretion
    • T cells incubated with LPS-stimulated CD11c+ dendritic cells with IL-23 in the presence of MOG peptide and neutralizing antibodies against IFN-gamma and IL-4 plus either IL-25 or IL-13 antibodies produce less IL-17 than with both IL-25 and IL-13 antibodies or without either   (MGI Ref ID J:125296)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Immunology, Inflammation and Autoimmunity Research
Autoimmunity
      experimental allergic encephalomyelitis (EAE)
Immunodeficiency
      multiple immune defects
Rearranged Antigen-Specific T Cell Receptor Transgenes

Research Tools
Immunology, Inflammation and Autoimmunity Research
      T Cell Receptor Transgenics
Sensorineural Research

Sensorineural Research
Eye Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tg(Tcra2D2,Tcrb2D2)1Kuch
Allele Name transgene insertion 1, Vijay Kuchroo
Allele Type Transgenic (Inserted expressed sequence)
Common Name(s) 2D2; 2D2 TCR; MOG TCR; MOGTCR; TCRMOG; TCRMOG;
Mutation Made By Vijay Kuchroo,   Brigham and Women's Hospital
Strain of OriginC57BL/6
Expressed Gene Tcra, T cell receptor alpha chain, mouse, laboratory
Expressed Gene Tcrb, T cell receptor beta chain, mouse, laboratory
Promoter Tcra, T cell receptor alpha chain, mouse, laboratory
Promoter Tcrb, T cell receptor beta chain, mouse, laboratory
Molecular Note The Valpha3.2Jalpha18 region of Tcra from the 2D2 T cell clone as well as the Vbeta11DJbeta1,1 region of Tcrb from the same 2D2 clone were inserted into TCR expression cassettes. The linearized plasmids were injected into the pronuclei of fertilized C57BL/6 oocytes. The 2D2 clone was purified from C57BL/6 mice immunized with a peptide from myelin oligodendrocyte glycoprotein (MOG). The amino acid sequence of the CDR3 region from TCR alpha is VYF CALRSY NFG and that from TCR beta is CASS LDCG ANP. [MGI Ref ID J:83278]
 
 

Genotyping

Genotyping Information

Genotyping Protocols

Tcra2d2,

MELT


Tg(Tcra2D2,Tcrb2D2)1Kuch, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Bettelli E; Pagany M; Weiner HL; Linington C; Sobel RA; Kuchroo VK. 2003. Myelin oligodendrocyte glycoprotein-specific T cell receptor transgenic mice develop spontaneous autoimmune optic neuritis. J Exp Med 197(9):1073-81. [PubMed: 12732654]  [MGI Ref ID J:83278]

Additional References

Tg(Tcra2D2,Tcrb2D2)1Kuch related

Anderson AC; Lord GM; Dardalhon V; Lee DH; Sabatos-Peyton CA; Glimcher LH; Kuchroo VK. 2010. T-bet, a Th1 transcription factor regulates the expression of Tim-3. Eur J Immunol 40(3):859-66. [PubMed: 20049876]  [MGI Ref ID J:157872]

Angiari S; Rossi B; Piccio L; Zinselmeyer BH; Budui S; Zenaro E; Della Bianca V; Bach SD; Scarpini E; Bolomini-Vittori M; Piacentino G; Dusi S; Laudanna C; Cross AH; Miller MJ; Constantin G. 2013. Regulatory T cells suppress the late phase of the immune response in lymph nodes through P-selectin glycoprotein ligand-1. J Immunol 191(11):5489-500. [PubMed: 24174617]  [MGI Ref ID J:207010]

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Murugaiyan G; Mittal A; Weiner HL. 2008. Increased osteopontin expression in dendritic cells amplifies IL-17 production by CD4+ T cells in experimental autoimmune encephalomyelitis and in multiple sclerosis. J Immunol 181(11):7480-8. [PubMed: 19017937]  [MGI Ref ID J:142205]

Nowak EC; de Vries VC; Wasiuk A; Ahonen C; Bennett KA; Le Mercier I; Ha DG; Noelle RJ. 2012. Tryptophan hydroxylase-1 regulates immune tolerance and inflammation. J Exp Med 209(11):2127-35. [PubMed: 23008335]  [MGI Ref ID J:190958]

O'Connell RM; Kahn D; Gibson WS; Round JL; Scholz RL; Chaudhuri AA; Kahn ME; Rao DS; Baltimore D. 2010. MicroRNA-155 promotes autoimmune inflammation by enhancing inflammatory T cell development. Immunity 33(4):607-19. [PubMed: 20888269]  [MGI Ref ID J:165652]

Oh K; Park HB; Seo MW; Byoun OJ; Lee DS. 2012. Transglutaminase 2 exacerbates experimental autoimmune encephalomyelitis through positive regulation of encephalitogenic T cell differentiation and inflammation. Clin Immunol 145(2):122-32. [PubMed: 23001131]  [MGI Ref ID J:190627]

Ortega SB; Kashi VP; Tyler AF; Cunnusamy K; Mendoza JP; Karandikar NJ. 2013. The disease-ameliorating function of autoregulatory CD8 T cells is mediated by targeting of encephalitogenic CD4 T cells in experimental autoimmune encephalomyelitis. J Immunol 191(1):117-26. [PubMed: 23733879]  [MGI Ref ID J:205352]

Perona-Wright G; Jenkins SJ; O'Connor RA; Zienkiewicz D; McSorley HJ; Maizels RM; Anderton SM; MacDonald AS. 2009. A pivotal role for CD40-mediated IL-6 production by dendritic cells during IL-17 induction in vivo. J Immunol 182(5):2808-15. [PubMed: 19234175]  [MGI Ref ID J:146255]

Peters A; Pitcher LA; Sullivan JM; Mitsdoerffer M; Acton SE; Franz B; Wucherpfennig K; Turley S; Carroll MC; Sobel RA; Bettelli E; Kuchroo VK. 2011. Th17 cells induce ectopic lymphoid follicles in central nervous system tissue inflammation. Immunity 35(6):986-96. [PubMed: 22177922]  [MGI Ref ID J:179284]

Petersen TR; Gulland S; Bettelli E; Kuchroo V; Palmer E; Backstrom BT. 2004. A chimeric T cell receptor with super-signaling properties. Int Immunol 16(7):889-94. [PubMed: 15148288]  [MGI Ref ID J:125963]

Petersen TR; Lata R; Spittle E; Backstrom BT. 2007. A chimeric TCR-beta chain confers increased susceptibility to EAE. Mol Immunol 44(14):3473-81. [PubMed: 17481734]  [MGI Ref ID J:125962]

Qin H; Yeh WI; De Sarno P; Holdbrooks AT; Liu Y; Muldowney MT; Reynolds SL; Yanagisawa LL; Fox TH 3rd; Park K; Harrington LE; Raman C; Benveniste EN. 2012. Signal transducer and activator of transcription-3/suppressor of cytokine signaling-3 (STAT3/SOCS3) axis in myeloid cells regulates neuroinflammation. Proc Natl Acad Sci U S A 109(13):5004-9. [PubMed: 22411837]  [MGI Ref ID J:182215]

Quintana FJ; Basso AS; Iglesias AH; Korn T; Farez MF; Bettelli E; Caccamo M; Oukka M; Weiner HL. 2008. Control of T(reg) and T(H)17 cell differentiation by the aryl hydrocarbon receptor. Nature 453(7191):65-71. [PubMed: 18362915]  [MGI Ref ID J:136052]

Reboldi A; Coisne C; Baumjohann D; Benvenuto F; Bottinelli D; Lira S; Uccelli A; Lanzavecchia A; Engelhardt B; Sallusto F. 2009. C-C chemokine receptor 6-regulated entry of TH-17 cells into the CNS through the choroid plexus is required for the initiation of EAE. Nat Immunol 10(5):514-23. [PubMed: 19305396]  [MGI Ref ID J:148286]

Rothhammer V; Heink S; Petermann F; Srivastava R; Claussen MC; Hemmer B; Korn T. 2011. Th17 lymphocytes traffic to the central nervous system independently of alpha4 integrin expression during EAE. J Exp Med 208(12):2465-76. [PubMed: 22025301]  [MGI Ref ID J:178760]

Sandy AR; Stoolman J; Malott K; Pongtornpipat P; Segal BM; Maillard I. 2013. Notch signaling regulates T cell accumulation and function in the central nervous system during experimental autoimmune encephalomyelitis. J Immunol 191(4):1606-13. [PubMed: 23825310]  [MGI Ref ID J:205709]

Sayed BA; Christy AL; Walker ME; Brown MA. 2010. Meningeal mast cells affect early T cell central nervous system infiltration and blood-brain barrier integrity through TNF: a role for neutrophil recruitment? J Immunol 184(12):6891-900. [PubMed: 20488789]  [MGI Ref ID J:161131]

Sestero CM; McGuire DJ; De Sarno P; Brantley EC; Soldevila G; Axtell RC; Raman C. 2012. CD5-dependent CK2 activation pathway regulates threshold for T cell anergy. J Immunol 189(6):2918-30. [PubMed: 22904299]  [MGI Ref ID J:189919]

Shinohara ML; Kim JH; Garcia VA; Cantor H. 2008. Engagement of the type I interferon receptor on dendritic cells inhibits T helper 17 cell development: role of intracellular osteopontin. Immunity 29(1):68-78. [PubMed: 18619869]  [MGI Ref ID J:137879]

Skarica M; Wang T; McCadden E; Kardian D; Calabresi PA; Small D; Whartenby KA. 2009. Signal transduction inhibition of APCs diminishes th17 and Th1 responses in experimental autoimmune encephalomyelitis. J Immunol 182(7):4192-9. [PubMed: 19299717]  [MGI Ref ID J:147124]

Sobottka B; Harrer MD; Ziegler U; Fischer K; Wiendl H; Hunig T; Becher B; Goebels N. 2009. Collateral bystander damage by myelin-directed CD8+ T cells causes axonal loss. Am J Pathol 175(3):1160-6. [PubMed: 19700745]  [MGI Ref ID J:153125]

Solomon BD; Mueller C; Chae WJ; Alabanza LM; Bynoe MS. 2011. Neuropilin-1 attenuates autoreactivity in experimental autoimmune encephalomyelitis. Proc Natl Acad Sci U S A 108(5):2040-5. [PubMed: 21245328]  [MGI Ref ID J:169116]

Sosa RA; Murphey C; Ji N; Cardona AE; Forsthuber TG. 2013. The kinetics of myelin antigen uptake by myeloid cells in the central nervous system during experimental autoimmune encephalomyelitis. J Immunol 191(12):5848-57. [PubMed: 24227784]  [MGI Ref ID J:207124]

Sotnikov I; Veremeyko T; Starossom SC; Barteneva N; Weiner HL; Ponomarev ED. 2013. Platelets recognize brain-specific glycolipid structures, respond to neurovascular damage and promote neuroinflammation. PLoS One 8(3):e58979. [PubMed: 23555611]  [MGI Ref ID J:199888]

Steinbach K; Piedavent M; Bauer S; Neumann JT; Friese MA. 2013. Neutrophils amplify autoimmune central nervous system infiltrates by maturing local APCs. J Immunol 191(9):4531-9. [PubMed: 24062488]  [MGI Ref ID J:206250]

Takahashi H; Kanno T; Nakayamada S; Hirahara K; Sciume G; Muljo SA; Kuchen S; Casellas R; Wei L; Kanno Y; O'Shea JJ. 2012. TGF-beta and retinoic acid induce the microRNA miR-10a, which targets Bcl-6 and constrains the plasticity of helper T cells. Nat Immunol 13(6):587-95. [PubMed: 22544395]  [MGI Ref ID J:186450]

Talla V; Yang C; Shaw G; Porciatti V; Koilkonda RD; Guy J. 2013. Noninvasive assessments of optic nerve neurodegeneration in transgenic mice with isolated optic neuritis. Invest Ophthalmol Vis Sci 54(7):4440-50. [PubMed: 23722393]  [MGI Ref ID J:214037]

Tsai VW; Mohammad MG; Tolhurst O; Breit SN; Sawchenko PE; Brown DA. 2011. CCAAT/Enhancer Binding Protein-delta Expression by Dendritic Cells Regulates CNS Autoimmune Inflammatory Disease. J Neurosci 31(48):17612-21. [PubMed: 22131422]  [MGI Ref ID J:178141]

Wang Y; Godec J; Ben-Aissa K; Cui K; Zhao K; Pucsek AB; Lee YK; Weaver CT; Yagi R; Lazarevic V. 2014. The transcription factors T-bet and Runx are required for the ontogeny of pathogenic interferon-gamma-producing T helper 17 cells. Immunity 40(3):355-66. [PubMed: 24530058]  [MGI Ref ID J:209915]

Withers DR; Jaensson E; Gaspal F; McConnell FM; Eksteen B; Anderson G; Agace WW; Lane PJ. 2009. The survival of memory CD4+ T cells within the gut lamina propria requires OX40 and CD30 signals. J Immunol 183(8):5079-84. [PubMed: 19786532]  [MGI Ref ID J:153824]

Wu C; Pot C; Apetoh L; Thalhamer T; Zhu B; Murugaiyan G; Xiao S; Lee Y; Rangachari M; Yosef N; Kuchroo VK. 2013. Metallothioneins negatively regulate IL-27-induced type 1 regulatory T-cell differentiation. Proc Natl Acad Sci U S A 110(19):7802-7. [PubMed: 23630250]  [MGI Ref ID J:197331]

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Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX10

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, hemizygous mice are bred to wildtype (noncarrier) siblings or to C57BL/6J inbred mice (Stock No. 000664).
Mating System+/+ sibling x Hemizygote         (Female x Male)   17-MAY-08
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $232.00Female or MaleHemizygous for Tg(Tcra2D2,Tcrb2D2)1Kuch  
Price per Pair (US dollars $)Pair Genotype
$304.00Hemizygous for Tg(Tcra2D2,Tcrb2D2)1Kuch x Noncarrier  
$304.00Noncarrier x Hemizygous for Tg(Tcra2D2,Tcrb2D2)1Kuch  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Cryopreserved

Frozen Products

Price (US dollars $)
Frozen Embryo $1650.00

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $301.60Female or MaleHemizygous for Tg(Tcra2D2,Tcrb2D2)1Kuch  
Price per Pair (US dollars $)Pair Genotype
$395.20Hemizygous for Tg(Tcra2D2,Tcrb2D2)1Kuch x Noncarrier  
$395.20Noncarrier x Hemizygous for Tg(Tcra2D2,Tcrb2D2)1Kuch  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Cryopreserved

Frozen Products

Price (US dollars $)
Frozen Embryo $2145.00

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Control Information

  Control
   Noncarrier
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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