Strain Name: |
B6.Cg-Sfpi1tm2Dgt/J |
|---|---|
Stock Number: |
006922 |
Availability: | Repository- Live |
General Terms and Conditions |
| Genes & Alleles | Sfpi1; Sfpi1tm2Dgt; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Donating Investigator Daniel Tenen, Beth Israel Deaconess Medical Center Generation N9+F1 (19-MAY-08) Strain Description
Mice that are homozygous for this "PU.1F" conditional allele are viable and fertile. When PU.1F mice are bred to mice expressing Cre recombinase, exons 4-5 of the targeted gene are deleted in the cre-expressing tissues in the offspring. These mice may be useful in studying T cell lymphoma, AML and other cancers, as well as transcription factors, hematopoiesis, and the development of multiple cell lineages.For example, when PU.1F mice are crossed with mice expressing the interferon- or dsRNA-inducible Mx1-cre transgenic mice (see Stock No. 003556), this mutant mouse strain may be useful in studies of hematopoietic stem cells.
Strain Development
A targeting vector was designed to insert loxp sites flanking exons 4-5 of the endogenous mouse PU.1 (or Sfpi1) gene, as well as a loxp-flanked PGK-neo cassette downstream of exon 5. This construct was electroporated into 129-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts, and the resulting chimeric males were bred to C57BL/6 females. These PU.1F mice were then backcrossed to C57BL/6 for at least 5 generations. At some point, these mice were bred with Mx1-Cre transgenic mice (congenic (N9) on a C57BL/6 background) and double mutant mice were subsequently sent to The Jackson Laboratory. Upon arrival, mice were selected for the PU.1F targeted mutation and (against the Mx1-Cre transgene) to establish this strain.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Sfpi1tm2Dgt | ||
|---|---|---|---|
| Allele Name | targeted mutation 2, Daniel G Tenen | ||
| Common Name(s) | PU.1F; | ||
| Mutation Made By | Daniel Tenen, Beth Israel Deaconess Medical Center | ||
| Gene Symbol and Name | Sfpi1, SFFV proviral integration 1 | ||
| Chromosome | 2 | ||
| Gene Common Name(s) | Dis-1; OF; PU.1; SPI-1; SPI-A; Sfpi-1; Tcfpu1; Tfpu.1; transcription factor PU.1; | ||
| Molecular Note | LoxP sites were inserted in intron 3 and a loxP-flanked neomycin selection cassette was inserted into intron 5. [MGI Ref ID J:114150] | ||
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Generic Cre
Sfpi1tm2Dgt
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, homozygous mice are bred. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying other alleles of Sfpi1
006099 B6;129-Sfpi1tm1.2Dgt/J 006147 B6;FVB-Tg(Sfpi1,-EGFP)7Dgt/J 006083 STOCK Sfpi1tm1.3Dgt/J View Strains carrying other alleles of Sfpi1 (3 strains)
Additional Web Information
Congenic Nomenclature
Cre-lox or FLP-FRT Systems
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
Cancer Research
Genes Regulating Growth and Proliferation
Increased Tumor Incidence (Leukemia)
Developmental Biology Research
Internal/Organ Defects (Lymphoid Tissue Defects)
Internal/Organ Defects (hematopoietic defects)
Hematological Research
Hematopoietic Defects
Immunology and Inflammation Research
Lymphoid Tissue Defects (hematopoietic development)
Internal/Organ Research
Lymphoid Tissue Defects
Research Tools
Cancer Research (Leukemia)
Cancer Research (tumor immunology)
Cre-lox System (loxP-flanked Sequences)
Hematological Research
Immunology and Inflammation Research
References
Selected Reference(s)
Additional ReferencesIwasaki H; Somoza C; Shigematsu H; Duprez EA; Iwasaki-Arai J; Mizuno S; Arinobu Y; Geary K; Zhang P; Dayaram T; Fenyus ML; Elf S; Chan S; Kastner P; Huettner CS; Murray R; Tenen DG; Akashi K. 2005. Distinctive and indispensable roles of PU.1 in maintenance of hematopoietic stem cells and their differentiation. Blood 106(5):1590-600. [PubMed: 15914556] [MGI Ref ID J:114150]
Price and Supply Information
| Strain Name: | B6.Cg-Sfpi1tm2Dgt/J |
| Stock Number: | 006922 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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