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| The single coding exon of the glucosaminyl (N-acetyl) transferase 3, mucin type (Gcnt3) gene is flanked by loxP sites in these mice. When bred to cre transgenic strains, the exon is excised to produce a null allele. | |||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Former Names B6.129-Gcnt2tm1Jxm/J (Changed: 25-OCT-07 ) Type Congenic; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Generation [?pN7]+F1 (06-MAY-08) Donating Investigator Jamey Marth, University of California at San Diego Description
Mice that are homozygous for the floxed targeted mutation are viable, normal in size, and do not display any gross physical or behavioral abnormalities. When bred to cre transgenic strains, the loxP-flanked exons are deleted by cre expression to produce a null allele.Development
A targeting vector was used to place a floxed neomycin-thymidine kinase expression cassette downstream of the single coding exon, and a loxP site upstream. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+-derived R1 embryonic stem (ES) cells. Transient cre expression in targeted cells excised the neo cassette and left the floxed exon. The line has been backcrossed to C57BL/6 at least 7 times by the donating laboratory.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Additional Web Information
Congenic Nomenclature
Cre-lox Systems
Phenotype
Phenotype Information
View Mammalian Phenotype Terms
Mammalian Phenotype Terms
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Gcnt3tm1Jxm/Gcnt3tm1Jxm
involves: 129S1/Sv * 129X1/SvJ * C57BL/6
- normal phenotype
- no abnormal phenotype detected (MGI Ref ID J:132967)
- viable and fertile with no gross behavioral or physical abnormalities
This mouse can be used to support research in many areas including:
Research Tools
Cre-lox System (loxP-flanked Sequences)
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Gcnt3tm1Jxm | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Jamey Marth | ||
| Allele Type | Targeted (Floxed/Frt) | ||
| Mutation Made By | Jamey Marth, University of California at San Diego | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| ES Cell Line Name | R1 | ||
| ES Cell Line Strain | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Gene Symbol and Name | Gcnt3, glucosaminyl (N-acetyl) transferase 3, mucin type | ||
| Chromosome | 9 | ||
| Gene Common Name(s) | 2010013H22Rik; 2210021I22Rik; 2210401J11Rik; C2/4GnT; C2GnT-M; C2GnT2; GnT-M; RIKEN cDNA 2010013H22 gene; RIKEN cDNA 2210021I22 gene; RIKEN cDNA 2210401J11 gene; beta-16-N-acetylglucosaminyltransferase; dI/C2/C4GnT; | ||
| Molecular Note | A targeting vector was used to place a floxed neomycin-thymidine kinase expression cassette downstream of the single coding exon, and a loxP site upstream. Transient Cre expression in targeted ES cells excised the neo cassette and left the floxed exon. [MGI Ref ID J:132967] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Gcnt3tm1Jxm, STD PCR, vers. 2
Helpful Links
Optimizing PCR Protocols
References
References
Additional References
Gcnt3tm1Jxm relatedMarth JD. 2008. Generation of a floxed Gcnt3 allele MGI Direct Data Submission :. [MGI Ref ID J:132967]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Mating System Homozygote x Homozygote (Female x Male) Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $155.70 Female or Male Homozygous for Gcnt3tm1Jxm *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $311.40 Homozygous for Gcnt3tm1Jxm x Homozygous for Gcnt3tm1Jxm
Additional Supply Details
| Supply Notes |
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| Pricing for International shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $202.50 Female or Male Homozygous for Gcnt3tm1Jxm *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $404.90 Homozygous for Gcnt3tm1Jxm x Homozygous for Gcnt3tm1Jxm
Additional Supply Details
| Supply Notes |
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
See Terms of Use
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form
Terms of Use
Terms of Use
General Terms and Conditions
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
Contact information
General inquiries
Contracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice & Services Conditions of Use
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