Strain Name: |
C57BL/6J-EgfrVel/J |
|---|---|
Stock Number: |
006926 |
Availability: | Repository- Live |
General Terms and Conditions |
| Genes & Alleles | Egfr; EgfrVel; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Chemically Induced Mutation Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Coisogenic Type JAX® GEMM® Strain - Mutant Strain Mating System +/+ sibling x Heterozygote (Female x Male) Species laboratory mouse Donating Investigator Bruce Beutler, The Scripps Research Institute Generation N11+ (04-DEC-07) Strain Description
Mice homozygous for this mutation have an embryonic lethal phenotype, failing to develop past embryonic days 13.5. Heterozygous mice are viable, fertile, do not display any behavioral abnormalities and are born with open eyelids and curly vibrissae. Adult heterozygotes often have small eyes and corneal opacity with excessive secretions at the eyelid edges. MEFs (mouse embryonic fibroblasts) exhibit reduced migratory ability, approximately 53% of wildtype controls. Histological analysis of homozygous E12.5 embryos reveals placental defects. This mutant mouse strain may be useful in studies of development and as a visible dominant marker for the Egfr allelic series.Strain Development
This strain was developed using ENU mutagenesis of male C57BL/6J mice. This mutation was identified in F1 offspring of the ENU-treated male founder. The ENU treatment induced an adenine-to-guanine transition, leading to an amino acid change from aspartic acid to glycine at position 833 of the protein (D833G). Mutant animals were backcrossed to C57BL/6J for at least 10 generations.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | EgfrVel | ||
|---|---|---|---|
| Allele Name | velvet | ||
| Mutation Made By | Bruce Beutler, The Scripps Research Institute | ||
| Strain of Origin | C57BL/6J | ||
| Gene Symbol and Name | Egfr, epidermal growth factor receptor | ||
| Chromosome | 11 | ||
| Gene Common Name(s) | 9030024J15Rik; AI552599; ERBB; ERBB1; ErbB-1; Erbb; Errp; HER1; PIG61; RIKEN cDNA 9030024J15 gene; Wa5; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; avian erythroblastosis oncogene B; expressed sequence AI552599; mENA; wa-2; wa2; waved 2; waved 5; | ||
| Molecular Note | An A to G transition in the coding region causes the replacement of aspartic acid by glycine at position 833 (D833G). The mutation causes an alteration in the cytoplasmic tyrosine kinase domain structure and falls within a DFG motif known to be importantfor ATP coordination. [MGI Ref ID J:88776] | ||
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, these mice are bred as heterozygotes. Homozygotes have an embryonic lethal phenotype. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying other alleles of Egfr
000553 B6EiC3Sn a/A-Egfrwa2 Wnt3avt/J 002857 STOCK Egfrtm1Mag/J 000317 STOCK a/a Egfrwa2/J View Strains carrying other alleles of Egfr (3 strains)
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
Dermatology Research
Skin and Hair Texture Defects
Developmental Biology Research
Embryonic Lethality (Homozygous)
Eye Defects
Skin and Hair Texture Defects
Sensorineural Research
Eye Defects
References
Selected Reference(s)
Du X; Tabeta K; Hoebe K; Liu H; Mann N; Mudd S; Crozat K; Sovath S; Gong X; Beutler B. 2004. Velvet, a dominant Egfr mutation that causes wavy hair and defective eyelid development in mice. Genetics 166(1):331-40. [PubMed: 15020428] [MGI Ref ID J:88776]
Price and Supply Information
| Strain Name: | C57BL/6J-EgfrVel/J |
| Stock Number: | 006926 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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