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Strain Name:

C57BL/6J-EgfrVel/J

Stock Number:

006926

Availability:

Repository- Live


General Terms and Conditions

Genes & Alleles   Egfr;   EgfrVel;


Product Information

Strain Details

Type JAX® GEMM® Strain - Chemically Induced Mutation
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Coisogenic
Type JAX® GEMM® Strain - Mutant Strain
Mating System+/+ sibling x Heterozygote         (Female x Male)
Specieslaboratory mouse
Donating Investigator Bruce Beutler,   The Scripps Research Institute
GenerationN11+ (04-DEC-07)

Strain Description
Mice homozygous for this mutation have an embryonic lethal phenotype, failing to develop past embryonic days 13.5. Heterozygous mice are viable, fertile, do not display any behavioral abnormalities and are born with open eyelids and curly vibrissae. Adult heterozygotes often have small eyes and corneal opacity with excessive secretions at the eyelid edges. MEFs (mouse embryonic fibroblasts) exhibit reduced migratory ability, approximately 53% of wildtype controls. Histological analysis of homozygous E12.5 embryos reveals placental defects. This mutant mouse strain may be useful in studies of development and as a visible dominant marker for the Egfr allelic series.

Strain Development
This strain was developed using ENU mutagenesis of male C57BL/6J mice. This mutation was identified in F1 offspring of the ENU-treated male founder. The ENU treatment induced an adenine-to-guanine transition, leading to an amino acid change from aspartic acid to glycine at position 833 of the protein (D833G). Mutant animals were backcrossed to C57BL/6J for at least 10 generations.

Mammalian Phenotype Terms assigned by genotype

EgfrVel/Egfr+

        involves: C57BL/6J
  • skin/coat/nails phenotype
  • waved hair (MGI Ref ID J:88776)
  • touch/vibrissae phenotype
  • curly vibrissae (MGI Ref ID J:88776)
  • vision/eye phenotype
  • eyelids open at birth (MGI Ref ID J:88776)

EgfrVel/EgfrVel

        involves: C57BL/6J
  • lethality-prenatal/perinatal
  • embryonic lethality during organogenesis (MGI Ref ID J:88776)
    • mice do not survive past E13.5

Gene & Allele Details

Allele Symbol EgfrVel
Allele Name velvet
Mutation Made By Bruce Beutler,   The Scripps Research Institute
Strain of OriginC57BL/6J
Gene Symbol and Name Egfr, epidermal growth factor receptor
Chromosome 11
Gene Common Name(s) 9030024J15Rik; AI552599; ERBB; ERBB1; ErbB-1; Erbb; Errp; HER1; PIG61; RIKEN cDNA 9030024J15 gene; Wa5; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; avian erythroblastosis oncogene B; expressed sequence AI552599; mENA; wa-2; wa2; waved 2; waved 5;
Molecular Note An A to G transition in the coding region causes the replacement of aspartic acid by glycine at position 833 (D833G). The mutation causes an alteration in the cytoplasmic tyrosine kinase domain structure and falls within a DFG motif known to be importantfor ATP coordination. [MGI Ref ID J:88776]

Control Information

  Control
   Wild-type from the colony
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice are bred as heterozygotes. Homozygotes have an embryonic lethal phenotype.
Diet Information LabDiet® 5K52/5K67

Related Strains

Strains carrying other alleles of Egfr
000553   B6EiC3Sn a/A-Egfrwa2 Wnt3avt/J
002857   STOCK Egfrtm1Mag/J
000317   STOCK a/a Egfrwa2/J
View Strains carrying other alleles of Egfr     (3 strains)

Animal Health Reports

Room Number           AX11

Research Applications

This mouse can be used to support research in many areas including:

Dermatology Research
Skin and Hair Texture Defects

Developmental Biology Research
Embryonic Lethality (Homozygous)
Eye Defects
Skin and Hair Texture Defects

Sensorineural Research
Eye Defects

References

Selected Reference(s)

Du X; Tabeta K; Hoebe K; Liu H; Mann N; Mudd S; Crozat K; Sovath S; Gong X; Beutler B. 2004. Velvet, a dominant Egfr mutation that causes wavy hair and defective eyelid development in mice. Genetics 166(1):331-40. [PubMed: 15020428]  [MGI Ref ID J:88776]


Price and Supply Information

Strain Name: C57BL/6J-EgfrVel/J
Stock Number: 006926

Price Details

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Supply Details

Standard SupplyRepository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes Usually shipped between four and eight weeks of age.
This strain is included in the Induced Mutant Resource Colony collection.
LicensingSee General Terms and Conditions below for Licensing and Use Restrictions  
Control InformationView Control Information in Strain Details.

General Terms and Conditions

View JAX® Mice & Services Conditions of Use.

For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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