Strain Name:

C57BL/6J-EgfrVel/J

Stock Number:

006926

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Availability:

Cryopreserved - Ready for recovery

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Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Chemically Induced Mutation; Coisogenic; Mutant Strain;
Additional information on Genetically Engineered and Mutant Mice.
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Specieslaboratory mouse
GenerationN10F6pN1
Generation Definitions
 
Donating Investigator Bruce Beutler,   University of Texas Southwestern Medical

Description
Mice homozygous for this mutation have an embryonic lethal phenotype, failing to develop past embryonic days 13.5. Heterozygous mice are viable, fertile, do not display any behavioral abnormalities and are born with open eyelids and curly vibrissae. Adult heterozygotes often have small eyes and corneal opacity with excessive secretions at the eyelid edges. MEFs (mouse embryonic fibroblasts) exhibit reduced migratory ability, approximately 53% of wildtype controls. Histological analysis of homozygous E12.5 embryos reveals placental defects. This mutant mouse strain may be useful in studies of development and as a visible dominant marker for the Egfr allelic series.

Development
This strain was developed using ENU mutagenesis of male C57BL/6J mice. This mutation was identified in F1 offspring of the ENU-treated male founder. The ENU treatment induced an adenine-to-guanine transition, leading to an amino acid change from aspartic acid to glycine at position 833 of the protein (D833G). Mutant animals were backcrossed to C57BL/6J for at least 10 generations.

Control Information

  Control
   Wild-type from the colony
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Egfr
010575   B6;SJL-Tg(tetO-Egfr*)2-9Jek/J
000553   B6EiC3Sn a/A-Egfrwa2 Wnt3avt/J
018473   STOCK Egfrtm1.1Tyj/J
002857   STOCK Egfrtm1Mag/J
025184   STOCK Egfrwa2/2J
View Strains carrying other alleles of Egfr     (5 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Inflammatory Skin and Bowel Disease, Neonatal, 2; NISBD2   (EGFR)
Lung Cancer   (EGFR)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

EgfrVel/Egfr+

        involves: C57BL/6J
  • vision/eye phenotype
  • abnormal eyelid morphology
    • excessive secretions are always observed at the edges of adult heterozygous mutant eyelids, possibly due to infection, drying, or trauma   (MGI Ref ID J:88776)
    • abnormal eyelid development
      • migration of eyelid epithelial cells appears to be defective   (MGI Ref ID J:88776)
      • failure of eyelid fusion
        • at E16.5, the upper and lower eyelids of wild-type embryos are fused in the midline, whereas those of heterozygous mutant embryos remain far apart due to defective migration of eyelid epithelial cells   (MGI Ref ID J:88776)
    • eyelids open at birth
      • heterozygotes are born with eyelids open, whereas the eyelids of wild-type littermates remain closed until 12 days after birth   (MGI Ref ID J:88776)
  • corneal opacity
    • the eyes of adult heterozygotes exhibit corneal opacity   (MGI Ref ID J:88776)
  • microphthalmia
    • the eyes of adult heterozygotes are often small   (MGI Ref ID J:88776)
  • cellular phenotype
  • decreased fibroblast cell migration
    • in vitro, mouse embryonic fibroblasts (MEFs) isolated from heterozygous embryos show ~47% reduction in their migratory ability relative to wild-type MEFs   (MGI Ref ID J:88776)
  • growth/size/body phenotype
  • weight loss
    • significant weight loss is observed with addition of 2% dextran sodium sulfate (DSS) to water, suggesting susceptibility to induced colitis   (MGI Ref ID J:166602)
  • integument phenotype
  • abnormal hair follicle orientation
    • after the first coat, pelage hair loses its waviness and appears disoriented, with individual shafts projecting in different directions   (MGI Ref ID J:88776)
  • curly vibrissae
    • heterozygotes are born with curly vibrissae   (MGI Ref ID J:88776)
  • plush coat
    • coats have a velvet-like texture   (MGI Ref ID J:88776)
  • waved hair
    • the first coat is wavy   (MGI Ref ID J:88776)

EgfrVel/EgfrVel

        involves: C57BL/6J
  • mortality/aging
  • complete embryonic lethality during organogenesis
    • homozygous mutant embryos do not survive past E13.5, putatively due to a placenta defect   (MGI Ref ID J:88776)
    • a significant reduction in embryonic viability is first noted at E11.5   (MGI Ref ID J:88776)
  • embryogenesis phenotype
  • abnormal trophoblast layer morphology
    • at E12.5, the labyrinth trophoblast layer appears disorganized   (MGI Ref ID J:88776)
    • however, the organization and number of trophoblast giant cells remain relatively normal   (MGI Ref ID J:88776)
  • placental labyrinth hypoplasia
    • at E12.5, homozygous mutant placentas show a significant reduction in cell number relative to wild-type and heterozygous controls   (MGI Ref ID J:88776)
    • a number of dead or dying cells are also observed   (MGI Ref ID J:88776)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Dermatology Research
Skin and Hair Texture Defects

Developmental Biology Research
Embryonic Lethality (Homozygous)
Eye Defects
Skin and Hair Texture Defects

Sensorineural Research
Eye Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol EgfrVel
Allele Name velvet
Allele Type Chemically induced (ENU)
Mutation Made By Bruce Beutler,   University of Texas Southwestern Medical
Strain of OriginC57BL/6J
Gene Symbol and Name Egfr, epidermal growth factor receptor
Chromosome 11
Gene Common Name(s) 9030024J15Rik; AI552599; ERBB; ERBB1; ErbB-1; Erbb; Errb1; Errp; HER1; NISBD2; PIG61; RIKEN cDNA 9030024J15 gene; Wa5; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; avian erythroblastosis oncogene B; expressed sequence AI552599; mENA; wa-2; wa2; waved 2; waved 5;
Molecular Note An A to G transition in the coding region causes the replacement of aspartic acid by glycine at position 833 (D833G). The mutation causes an alteration in the cytoplasmic tyrosine kinase domain structure and falls within a DFG motif known to be importantfor ATP coordination. [MGI Ref ID J:88776]

Genotyping

Genotyping Information


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Du X; Tabeta K; Hoebe K; Liu H; Mann N; Mudd S; Crozat K; Sovath S; Gong X; Beutler B. 2004. Velvet, a dominant Egfr mutation that causes wavy hair and defective eyelid development in mice. Genetics 166(1):331-40. [PubMed: 15020428]  [MGI Ref ID J:88776]

Additional References

EgfrVel related

Andric N; Thomas M; Ascoli M. 2010. Transactivation of the epidermal growth factor receptor is involved in the lutropin receptor-mediated down-regulation of ovarian aromatase expression in vivo. Mol Endocrinol 24(3):552-60. [PubMed: 20093417]  [MGI Ref ID J:157693]

Brandl K; Sun L; Neppl C; Siggs OM; Le Gall SM; Tomisato W; Li X; Du X; Maennel DN; Blobel CP; Beutler B. 2010. MyD88 signaling in nonhematopoietic cells protects mice against induced colitis by regulating specific EGF receptor ligands. Proc Natl Acad Sci U S A 107(46):19967-72. [PubMed: 21041656]  [MGI Ref ID J:166602]

Mustafi R; Dougherty U; Shah H; Dehghan H; Gliksberg A; Wu J; Zhu H; Joseph L; Hart J; Dive C; Fichera A; Threadgill D; Bissonnette M. 2012. Both stromal cell and colonocyte epidermal growth factor receptors control HCT116 colon cancer cell growth in tumor xenografts. Carcinogenesis 33(10):1930-9. [PubMed: 22791816]  [MGI Ref ID J:192954]

Nakamura M; Schneider MR; Schmidt-Ullrich R; Paus R. 2013. Mutant laboratory mice with abnormalities in hair follicle morphogenesis, cycling, and/or structure: an update. J Dermatol Sci 69(1):6-29. [PubMed: 23165165]  [MGI Ref ID J:196518]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice are bred as heterozygotes. Homozygotes have an embryonic lethal phenotype.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2140.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2782.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Wild-type from the colony
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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