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| FUT1-deficient mice do not have epididymal alpha(1,2)fucosylated glycans or the fucosylated glycolipid, fucosyl GA1, in pancreatic acinar glands. Homozygotes exhibit delayed maturation of nerve fibers in the glomerular layer of the olfactory bulb. This mutant mouse strain may be useful in studies of glycosidic molecular interactions and function, and olfactory nerve pathway development. | ||||||||||||||||
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Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Donating Investigator Steven Domino, University of Michigan Description
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any behavioral abnormalities. Homozygous mice may be identified by their agouti and chinchilla coat color. Alpha(1,2)fucosylated glycans are not detected in the epididymis of homozygotes. Fucosylated glycolipids (fucosyl GA1) are not detected in the pancreatic acinar glands of homozygotes. Homozygotes exhibit delayed maturation of nerve fibers in the glomerular layer of the olfactory bulb due to absence of cell surface carbohydrate, blood group H carbohydrate, expression in primary sensory neurons. The Donating Investigator reports that the beta-galatosidase is expressed. This mutant mouse strain may be useful in studies of glycosidic molecular interactions and function, and olfactory nerve pathway development.This strain was transferred from the collection of the Consortium for Functional Glycomics.
Development
A targeting vector containing neomycin resistance and beta-galactosidase genes was used to disrupt the one coding exon. The construct was electroporated into 129X1/SvJ derived Pat5 OR (129X1/SvJ x 129S1/Sv)F1-Kitl+ derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient C57BL/6J blastocysts. The resulting male chimeric animals were crossed to C57BL/6J female mice. Heterozygotes were crossed to obtain homozygotes. The mice were then backcrossed to C57BL/6 for 12 generations before arriving at The Jackson Laboratory. The mice were crossed once to C57BL/6J upon arrival at The Jackson Laboratory.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
lacZ Expression Strains
View lacZ Expression Strains (198 strains)
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Fut1tm1Sdo/Fut1tm1Sdo
involves: 129X1/SvJ * C57BL/6J
- reproductive system phenotype
- abnormal male reproductive system physiology (MGI Ref ID J:72946)
- absence of fucosylated glycans in the epididymal epithelium but normal male fertility
- digestive/alimentary phenotype
- abnormal pancreas physiology (MGI Ref ID J:89929)
- absence of fucosylated glycolipids
- endocrine/exocrine gland phenotype
- abnormal pancreas physiology (MGI Ref ID J:89929)
- absence of fucosylated glycolipids
This mouse can be used to support research in many areas including:
Cell Biology Research
Protein Processing
Developmental Biology Research
Neurodevelopmental Defects
Neurobiology Research
Neurodevelopmental Defects
Research Tools
lacZ Expression
Cell Biology Research
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Fut1tm1Sdo | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Steven E Domino | ||
| Allele Type | Targeted (Reporter) | ||
| Common Name(s) | FUT1-; | ||
| Mutation Made By | Peter Sobieszczuk, Consortium for Functional Glycomics,TSRI | ||
| Strain of Origin | 129 | ||
| ES Cell Line Name | Other (see notes) | ||
| ES Cell Line Strain | 129 | ||
| Site of Expression | lacZ expression patterns have not been characterized. | ||
| Gene Symbol and Name | Fut1, fucosyltransferase 1 | ||
| Chromosome | 7 | ||
| Gene Common Name(s) | Futa; H; H transferase; HH; HSC; | ||
| General Note | ES cell line = Pat5 (129X1/SvJ) or R1 ((129X1/SvJ x 129S1/Sv)F1-Kitl+). | ||
| Molecular Note | Most of the single coding exon of the gene was replaced with a lacZ-PGK-neo cassette via homologous recombination resulting in deletion of the catalytic domain. Homozygous mutant animals were identified by Southern blot and PCR genotype analysis. [MGI Ref ID J:72946] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Fut1tm1Sdo, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Domino SE; Zhang L; Gillespie PJ; Saunders TL; Lowe JB. 2001. Deficiency of Reproductive Tract alpha(1,2)Fucosylated Glycans and Normal Fertility in Mice with Targeted Deletions of the FUT1 or FUT2 alpha(1,2)Fucosyltransferase Locus. Mol Cell Biol 21(24):8336-45. [PubMed: 11713270] [MGI Ref ID J:72946]
Additional References
Fut1tm1Sdo relatedHurd EA; Domino SE. 2004. Increased susceptibility of secretor factor gene Fut2-null mice to experimental vaginal candidiasis. Infect Immun 72(7):4279-81. [PubMed: 15213174] [MGI Ref ID J:90993]
Iwamori M; Domino SE. 2004. Tissue-specific loss of fucosylated glycolipids in mice with targeted deletion of alpha(1,2)fucosyltransferase genes. Biochem J 380(Pt 1):75-81. [PubMed: 14967068] [MGI Ref ID J:89929]
St John JA; Claxton C; Robinson MW; Yamamoto F; Domino SE; Key B. 2006. Genetic manipulation of blood group carbohydrates alters development and pathfinding of primary sensory axons of the olfactory systems. Dev Biol 298(2):470-84. [PubMed: 16884711] [MGI Ref ID J:119285]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice can be bred as homozygotes. Homozygous mice may be identified by their agouti and chinchilla coat color.
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Animals Provided
Price (US dollars $) Cryorecovery Fee $1900.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
| Pricing for International shipping destinations |
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Animals Provided
Price (US dollars $) Cryorecovery Fee $2470.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
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Supply Details
| Standard Supply | Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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