Description

Strain Information

Type Coisogenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Homozygote x Homozygote         (Female x Male)   18-NOV-08 Species laboratory mouse Generation F?+2 (12-JAN-09)   Donating Investigator Lars Nitschke,   Max-Planck Institute for Immunobiology

Description
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No expression of the targeted gene's protein product is detected on the cell surface, as determined by flow cytometry analysis of splenocytes from homozygotes. Mutants exhibit a reduction in recirculating mature B cells in the bone marrow and fewer transitional B cells in the spleen. Splenic and peripheral B cells express lower levels of membrane bound IgM than wildtype. B cell activation is abnormal in mutant mice, with enhanced Ca²⁺ mobilization after stimulation. Lipopolysaccaharide (LPS) challenge results in an increased proliferative response. CD22 deficient B cells have a shorter than average lifespan when compared to wildtype B cells. T-cell independent immune responses are impaired. This mutant mouse strain may be useful in studies of glycosidic molecular interactions and function, B cell development and T cell independent immune response.

This strain was transferred from the collection of the Consortium for Functional Glycomics.

Development
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt exon11, which encodes the transmembrane domain. The construct was electroporated into C57BL/6 derived BL/6-III embryonic stem (ES) cells.

Correctly targeted ES cells were injected into recipient blastocysts. The mice were backcrossed to the C57BL/6 background for 10 generations before arriving at The Jackson Laboratory.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Cd22^tm1Lam/Cd22^tm1Lam

        involves: 129 * C57BL/6

• immune system phenotype
• abnormal B cell activation (MGI Ref ID J:125719)
  • enhanced Ca²⁺ mobilization upon activation of B cells
• decreased mature B cell number (MGI Ref ID J:125719)
  • reduction of recirculating mature B cells in the bone marrow
• decreased marginal zone B cell number (MGI Ref ID J:125719)
  • reduced by 20%
• hematopoietic system phenotype
• abnormal B cell activation (MGI Ref ID J:125719)
  • enhanced Ca²⁺ mobilization upon activation of B cells
• decreased mature B cell number (MGI Ref ID J:125719)
  • reduction of recirculating mature B cells in the bone marrow
• decreased marginal zone B cell number (MGI Ref ID J:125719)
  • reduced by 20%

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Cell Biology Research
Protein Processing

Immunology and Inflammation Research
Immunodeficiency
      B cell deficiency

Genes & Alleles

Gene & Allele Information

Allele Symbol		Cd22tm1Lam
Allele Name		targeted mutation 1, Marinus C Lamers
Allele Type		Targeted (knock-out)
Common Name(s)		CD22-;
Mutation Made By		Peter Sobieszczuk,   Consortium for Functional Glycomics,TSRI
Strain of Origin		C57BL/6
ES Cell Line Name		BL/6-III
ES Cell Line Strain		C57BL/6
Gene Symbol and Name		Cd22, CD22 antigen
Chromosome		7
Gene Common Name(s)		B-lymphocyte antigen 8; FLJ22814; Lyb-8; Lyb8; MGC130020; SIGLEC-2; SIGLEC2;
Molecular Note		Exon 11, encoding the transmembrane domain, was disrupted by the insertion of a neomycin cassette. Flow cytometric analysis showed an absence of surface expression on splenocytes isolated from homozygous mutant mice. [MGI Ref ID J:38644]

Genotyping

Genotyping Information

Genotyping Protocols

Cd22^tm1Lam, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References

Selected Reference(s)

Nitschke L; Carsetti R; Ocker B; Kohler G; Lamers MC. 1997. CD22 is a negative regulator of B-cell receptor signalling. Curr Biol 7(2):133-43. [PubMed: 9016707]  [MGI Ref ID J:38644]

Additional References

Cd22^tm1Lam related

Amano H; Amano E; Moll T; Marinkovic D; Ibnou-Zekri N; Martinez-Soria E; Semac I; Wirth T; Nitschke L; Izui S. 2003. The yaa mutation promoting murine lupus causes defective development of marginal zone B cells. J Immunol 170(5):2293-301. [PubMed: 12594250]  [MGI Ref ID J:82017]

Ashton AW; Mukherjee S; Nagajyothi FN; Huang H; Braunstein VL; Desruisseaux MS; Factor SM; Lopez L; Berman JW; Wittner M; Scherer PE; Capra V; Coffman TM; Serhan CN; Gotlinger K; Wu KK; Weiss LM; Tanowitz HB. 2007. Thromboxane A2 is a key regulator of pathogenesis during Trypanosoma cruzi infection. J Exp Med 204(4):929-40. [PubMed: 17420269]  [MGI Ref ID J:125718]

Collins BE; Smith BA; Bengtson P; Paulson JC. 2006. Ablation of CD22 in ligand-deficient mice restores B cell receptor signaling. Nat Immunol 7(2):199-206. [PubMed: 16369536]  [MGI Ref ID J:112390]

Ghosh S; Bandulet C; Nitschke L. 2006. Regulation of B cell development and B cell signalling by CD22 and its ligands {alpha}2,6-linked sialic acids. Int Immunol 18(4):603-11. [PubMed: 16497829]  [MGI Ref ID J:107062]

Grewal PK; Boton M; Ramirez K; Collins BE; Saito A; Green RS; Ohtsubo K; Chui D; Marth JD. 2006. ST6Gal-I restrains CD22-dependent antigen receptor endocytosis and Shp-1 recruitment in normal and pathogenic immune signaling. Mol Cell Biol 26(13):4970-81. [PubMed: 16782884]  [MGI Ref ID J:110325]

Lajaunias F; Nitschke L; Moll T; Martinez-Soria E; Semac I; Chicheportiche Y; Parkhouse RM; Izui S. 2002. Differentially regulated expression and function of CD22 in activated B-1 and B-2 lymphocytes. J Immunol 168(12):6078-83. [PubMed: 12055217]  [MGI Ref ID J:123796]

Moll T; Nitschke L; Carroll M; Ravetch JV; Izui S. 2004. A critical role for Fc gamma RIIB in the induction of rheumatoid factors. J Immunol 173(7):4724-8. [PubMed: 15383609]  [MGI Ref ID J:93718]

Nitschke L; Floyd H; Ferguson DJ; Crocker PR. 1999. Identification of CD22 ligands on bone marrow sinusoidal endothelium implicated in CD22-dependent homing of recirculating B cells. J Exp Med 189(9):1513-8. [PubMed: 10224292]  [MGI Ref ID J:76972]

Nitschke L; Lajaunias F; Moll T; Ho L; Martinez-Soria E; Kikuchi S; Santiago-Raber ML; Dix C; Parkhouse RM; Izui S. 2006. Expression of aberrant forms of CD22 on B lymphocytes in Cd22a lupus-prone mice affects ligand binding. Int Immunol 18(1):59-68. [PubMed: 16291654]  [MGI Ref ID J:104168]

Samardzic T; Gerlach J; Muller K; Marinkovic D; Hess J; Nitschke L; Wirth T. 2002. CD22 regulates early B cell development in BOB.1/OBF.1-deficient mice. Eur J Immunol 32(9):2481-9. [PubMed: 12207332]  [MGI Ref ID J:78999]

Samardzic T; Marinkovic D; Danzer CP; Gerlach J; Nitschke L; Wirth T. 2002. Reduction of marginal zone B cells in CD22-deficient mice. Eur J Immunol 32(2):561-7. [PubMed: 11828373]  [MGI Ref ID J:74752]

Waisman A; Kraus M; Seagal J; Ghosh S; Melamed D; Song J; Sasaki Y; Classen S; Lutz C; Brombacher F; Nitschke L; Rajewsky K. 2007. IgG1 B cell receptor signaling is inhibited by CD22 and promotes the development of B cells whose survival is less dependent on Ig alpha/beta. J Exp Med 204(4):747-58. [PubMed: 17420268]  [MGI Ref ID J:125719]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX12

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, these mice can be bred as homozygotes.
Mating System	Homozygote x Homozygote         (Female x Male)   18-NOV-08
Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement.

Supply Notes

Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Terms of Use

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General Terms and Conditions

For Licensing and Use Restrictions view the link(s) below:
- Strain(s) not available to companies or for-profit entities.

Contact information

General inquiries

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phone:	207-288-6470
fax:	207-288-6655

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