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Genes & Alleles

Cd22;   Cd22tm1Lam;

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Product Information

Strain Details

Type JAX® GEMM® Strain - Coisogenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Mating System Heterozygote x Heterozygote         (Female x Male) Species laboratory mouse Donating Investigator Lars Nitschke,   Max-Planck Institute for Immunobiology Generation F?+ (21-MAY-08)

Strain Description
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No expression of the targeted gene's protein product is detected on the cell surface, as determined by flow cytometry analysis of splenocytes from homozygotes. Mutants exhibit a reduction in recirculating mature B cells in the bone marrow and fewer transitional B cells in the spleen. Splenic and peripheral B cells express lower levels of membrane bound IgM than wildtype. B cell activation is abnormal in mutant mice, with enhanced Ca²⁺ mobilization after stimulation. Lipopolysaccaharide (LPS) challenge results in an increased proliferative response. CD22 deficient B cells have a shorter than average lifespan when compared to wildtype B cells. T-cell independent immune responses are impaired. This mutant mouse strain may be useful in studies of glycosidic molecular interactions and function, B cell development and T cell independent immune response.

This strain was transferred from the collection of the Consortium for Functional Glycomics.

Strain Development
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt exon11, which encodes the transmembrane domain. The construct was electroporated into C57BL/6 derived BL/6-III embryonic stem (ES) cells.

Correctly targeted ES cells were injected into recipient blastocysts. The mice were backcrossed to the C57BL/6 background for 10 generations before arriving at The Jackson Laboratory.

Mammalian Phenotype Terms assigned by genotype The following phenotype information may relate to a genetic background differing from this JAX® Mice strain. Cd22tm1Lam/Cd22tm1Lam         involves: 129 * C57BL/6 immune system phenotype abnormal B cell activation (MGI Ref ID J:125719) enhanced Ca2+ mobilization upon activation of B cells decreased mature B cell number (MGI Ref ID J:125719) reduction of recirculating mature B cells in the bone marrow decreased marginal zone B cell number (MGI Ref ID J:125719) reduced by 20% hematopoietic system phenotype abnormal B cell activation (MGI Ref ID J:125719) enhanced Ca2+ mobilization upon activation of B cells decreased mature B cell number (MGI Ref ID J:125719) reduction of recirculating mature B cells in the bone marrow decreased marginal zone B cell number (MGI Ref ID J:125719) reduced by 20%

Gene & Allele Details

Allele Symbol		Cd22tm1Lam
Allele Name		targeted mutation 1, Marinus C Lamers
Common Name(s)		CD22-;
Mutation Made By		Peter Sobieszczuk,   Consortium for Functional Glycomics,TSRI
Strain of Origin		C57BL/6
ES Cell Line Name		BL/6-III
ES Cell Line Strain		C57BL/6
Gene Symbol and Name		Cd22, CD22 antigen
Chromosome		7
Gene Common Name(s)		B-lymphocyte antigen 8; FLJ22814; Lyb-8; Lyb8; MGC130020; SIGLEC-2; SIGLEC2;
Molecular Note		Exon 11, encoding the transmembrane domain, was disrupted by the insertion of a neomycin cassette. Flow cytometric analysis showed an absence of surface expression on splenocytes isolated from homozygous mutant mice. [MGI Ref ID J:38644]

Control Information

	Control
	Wild-type from the colony
	000664 C57BL/6J
Considerations for Choosing Controls

Genotyping Protocols

Cd22^tm1Lam

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, these mice can be bred as homozygotes.
Diet Information	LabDiet® 5K52/5K67

Animal Health Reports

Room Number           AX12

Research Applications

This mouse can be used to support research in many areas including:

Cell Biology Research
Protein Processing

Immunology and Inflammation Research
Immunodeficiency (B cell deficiency)

References

Selected Reference(s)

Nitschke L; Carsetti R; Ocker B; Kohler G; Lamers MC. 1997. CD22 is a negative regulator of B-cell receptor signalling. Curr Biol 7(2):133-43. [PubMed: 9016707]  [MGI Ref ID J:38644]

Additional References

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Price and Supply Information

Strain Name:	C57BL/6-Cd22tm1Lam /J
Stock Number:	006940

Price Details

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Supply Details

Standard Supply	Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes	Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection.
Licensing	See General Terms and Conditions below for Licensing and Use Restrictions
Control Information	View Control Information in Strain Details.

General Terms and Conditions

View JAX^® Mice & Services Conditions of Use.

For additional Licensing and Use Restrictions view the link(s) below:
- Strain(s) not available to companies or for-profit entities.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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