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| CD33 deficient mice exhibit a slight decrease in the mean erythrocyte count and hematocrit and an increase in the mean concentration of serum aspartate aminotransaminase. Experimentally induced peritonitis and systemic inflammation results in reduced immunological response. This mutant mouse strain may be useful in studies of glycosidic molecular interactions and function, hematopoiesis and immune response. | |||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Generation N14+N1F3 (18-DEC-08) Donating Investigator Ajit Varki, University of California, San Diego Description
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No expression of the targeted gene's protein product is detected on the cell surface, as determined by flow cytometry analysis of hematopoietic cell populations in blood and lymphoid organs from homozygotes. Homozygotes exhibit a slight decrease in the mean erythrocyte count and hematocrit and an increase in the mean concentration of serum aspartate aminotransaminase. Experimentally induced peritonitis and systemic inflammation results in reduced immunological response. This mutant mouse strain may be useful in studies of glycosidic molecular interactions and function, hematopoiesis and immune response.This strain was transferred from the collection of the Consortium for Functional Glycomics.
Development
A targeting vector containing a PGK-neomycin resistance cassette was used to disrupt 3.8kb of sequence encoding exons 1 through 5. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+ derived R1 embryonic stem (ES) cells.Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice, and then backcrossed to the same for 13 generations before arriving at The Jackson Laboratory.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Phenotype
Phenotype Information
View Mammalian Phenotype Terms
Mammalian Phenotype Terms
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Cd33tm1Ajv/Cd33tm1Ajv
involves: 129X1/SvJ * C57BL/6
- hematopoietic system phenotype
- abnormal erythrocyte cell number (MGI Ref ID J:83728)
- mean erythrocyte count and hematocrit were reduced very slightly
- homeostasis/metabolism phenotype
- abnormal circulating enzyme level (MGI Ref ID J:83728)
- the mean concentration of aspartate aminotransaminase was increased
- immune system phenotype
- decreased inflammatory response (MGI Ref ID J:83728)
- slight and inconsistent hyporesponsiveness to casein induced peritonitis
- marginally significant weaker IL-6 response to LPS-induced systemic inflammation
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Cd33tm1Ajv | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Ajit Varki | ||
| Allele Type | Targeted (knock-out) | ||
| Common Name(s) | Cd33-; | ||
| Mutation Made By | Peter Sobieszczuk, Consortium for Functional Glycomics,TSRI | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| ES Cell Line Name | R1 | ||
| ES Cell Line Strain | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Gene Symbol and Name | Cd33, CD33 antigen | ||
| Chromosome | 7 | ||
| Gene Common Name(s) | FLJ00391; SIGLEC-3; SIGLEC3; gp67; p67; | ||
| Molecular Note | Exons 1 through 5 were replaced by a pPGK-neo-bpA cassette. Homologous recombination was confirmed by PCR and Southern blot. Loss of gene expression was confirmed by flow cytometric analysis of hematopoietic cells. [MGI Ref ID J:83728] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Cd33tm1Ajv, SEP PCR, vers. 1
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Brinkman-Van Der Linden EC; Angata T; Reynolds SA; Powell LD; Hedrick SM; Varki A. 2003. CD33/Siglec-3 Binding Specificity, Expression Pattern, and Consequences of Gene Deletion in Mice. Mol Cell Biol 23(12):4199-206. [PubMed: 12773563] [MGI Ref ID J:83728]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice can be bred as homozygotes. Mating System Homozygote x Homozygote (Female x Male) Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $160.40 Female or Male Homozygous for Cd33tm1Ajv
Pairs /Price (US dollars $) Pair Genotype $320.80 Homozygous for Cd33tm1Ajv x Homozygous for Cd33tm1Ajv
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $208.60 Female or Male Homozygous for Cd33tm1Ajv
Pairs /Price (US dollars $) Pair Genotype $417.10 Homozygous for Cd33tm1Ajv x Homozygous for Cd33tm1Ajv
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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Contact Information
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Tel: 1-800-422-6423 or 1-207-288-5845
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Terms of Use
Terms of Use
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For Licensing and Use Restrictions view the link(s) below:
- Strain(s) not available to companies or for-profit entities.
Contact information
General inquiries
Contracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
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