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Genes & Alleles

Cd33;   Cd33tm1Ajv;

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Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Mating System Homozygote x Homozygote         (Female x Male) Species laboratory mouse Donating Investigator Ajit Varki,   University of California, San Diego Generation N14+ (12-MAY-08)

Strain Description
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No expression of the targeted gene's protein product is detected on the cell surface, as determined by flow cytometry analysis of hematopoietic cell populations in blood and lymphoid organs from homozygotes. Homozygotes exhibit a slight decrease in the mean erythrocyte count and hematocrit and an increase in the mean concentration of serum aspartate aminotransaminase. Experimentally induced peritonitis and systemic inflammation results in reduced immunological response. This mutant mouse strain may be useful in studies of glycosidic molecular interactions and function, hematopoiesis and immune response.

This strain was transferred from the collection of the Consortium for Functional Glycomics.

Strain Development
A targeting vector containing a PGK-neomycin resistance cassette was used to disrupt 3.8kb of sequence encoding exons 1 through 5. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl⁺ derived R1 embryonic stem (ES) cells.

Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice, and then backcrossed to the same for 13 generations before arriving at The Jackson Laboratory.

Mammalian Phenotype Terms assigned by genotype The following phenotype information may relate to a genetic background differing from this JAX® Mice strain. Cd33tm1Ajv/Cd33tm1Ajv         involves: 129X1/SvJ * C57BL/6 hematopoietic system phenotype abnormal erythrocyte cell number (MGI Ref ID J:83728) mean erythrocyte count and hematocrit were reduced very slightly homeostasis/metabolism phenotype abnormal circulating enzyme level (MGI Ref ID J:83728) the mean concentration of aspartate aminotransaminase was increased immune system phenotype decreased inflammatory response (MGI Ref ID J:83728) slight and inconsistent hyporesponsiveness to casein induced peritonitis marginally significant weaker IL-6 response to LPS-induced systemic inflammation

Gene & Allele Details

Allele Symbol		Cd33tm1Ajv
Allele Name		targeted mutation 1, Ajit Varki
Common Name(s)		Cd33-;
Mutation Made By		Peter Sobieszczuk,   Consortium for Functional Glycomics,TSRI
Strain of Origin		(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
ES Cell Line Name		R1
ES Cell Line Strain		(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Gene Symbol and Name		Cd33, CD33 antigen
Chromosome		7
Gene Common Name(s)		FLJ00391; SIGLEC-3; SIGLEC3; gp67; p67;
Molecular Note		Exons 1 through 5 were replaced by a pPGK-neo-bpA cassette. Homologous recombination was confirmed by PCR and Southern blot. Loss of gene expression was confirmed by flow cytometric analysis of hematopoietic cells. [MGI Ref ID J:83728]

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Genotyping Protocols

Cd33^tm1Ajv

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, these mice can be bred as homozygotes.
Diet Information	LabDiet® 5K52/5K67

Additional Web Information

Congenic Nomenclature

Animal Health Reports

Room Number           AX11

Research Applications

This mouse can be used to support research in many areas including:

Cell Biology Research
Protein Processing

Developmental Biology Research
Lymphoid Tissue Defects (hematopoietic defects)

Hematological Research
Hematopoietic Defects

Immunology and Inflammation Research
Immunodeficiency

References

Selected Reference(s)

Brinkman-Van Der Linden EC; Angata T; Reynolds SA; Powell LD; Hedrick SM; Varki A. 2003. CD33/Siglec-3 Binding Specificity, Expression Pattern, and Consequences of Gene Deletion in Mice. Mol Cell Biol 23(12):4199-206. [PubMed: 12773563]  [MGI Ref ID J:83728]

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Price and Supply Information

Strain Name:	B6.129-Cd33tm1Ajv/J
Stock Number:	006942

Price Details

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Supply Details

Standard Supply	Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes	Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection.
Licensing	See General Terms and Conditions below for Licensing and Use Restrictions
Control Information	View Control Information in Strain Details.

General Terms and Conditions

View JAX^® Mice & Services Conditions of Use.

For additional Licensing and Use Restrictions view the link(s) below:
- Strain(s) not available to companies or for-profit entities.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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