Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N1F9pN1   Donating Investigator Ajit Varki,   University of California, San Diego

Description
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No expression of the targeted gene's protein product is detected on the cell surface, as determined by flow cytometry analysis of hematopoietic cell populations in blood and lymphoid organs from homozygotes. Homozygotes exhibit a slight decrease in the mean erythrocyte count and hematocrit and an increase in the mean concentration of serum aspartate aminotransaminase. Experimentally induced peritonitis and systemic inflammation results in reduced immunological response. This mutant mouse strain may be useful in studies of glycosidic molecular interactions and function, hematopoiesis and immune response.

This strain was transferred from the collection of the Consortium for Functional Glycomics.

Development
A targeting vector containing a PGK-neomycin resistance cassette was used to disrupt 3.8kb of sequence encoding exons 1 through 5. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl⁺ derived R1 embryonic stem (ES) cells.

Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice, and then backcrossed to the same for 13 generations before arriving at The Jackson Laboratory.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Cd33^tm1Ajv/Cd33^tm1Ajv

        involves: 129X1/SvJ * C57BL/6

• hematopoietic system phenotype
• abnormal erythrocyte cell number (MGI Ref ID J:83728)
  • mean erythrocyte count and hematocrit were reduced very slightly
• homeostasis/metabolism phenotype
• abnormal circulating enzyme level (MGI Ref ID J:83728)
  • the mean concentration of aspartate aminotransaminase was increased
• immune system phenotype
• decreased inflammatory response (MGI Ref ID J:83728)
  • slight and inconsistent hyporesponsiveness to casein induced peritonitis
  • marginally significant weaker IL-6 response to LPS-induced systemic inflammation

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Cell Biology Research
Protein Processing

Developmental Biology Research
Lymphoid Tissue Defects
      hematopoietic defects

Hematological Research
Hematopoietic Defects

Immunology and Inflammation Research
Immunodeficiency

Genes & Alleles

Gene & Allele Information

Allele Symbol		Cd33tm1Ajv
Allele Name		targeted mutation 1, Ajit Varki
Allele Type		Targeted (knock-out)
Common Name(s)		Cd33-;
Mutation Made By		Peter Sobieszczuk,   Consortium for Functional Glycomics,TSRI
Strain of Origin		(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
ES Cell Line Name		R1
ES Cell Line Strain		(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Gene Symbol and Name		Cd33, CD33 antigen
Chromosome		7
Gene Common Name(s)		FLJ00391; SIGLEC-3; SIGLEC3; gp67; p67;
Molecular Note		Exons 1 through 5 were replaced by a pPGK-neo-bpA cassette. Homologous recombination was confirmed by PCR and Southern blot. Loss of gene expression was confirmed by flow cytometric analysis of hematopoietic cells. [MGI Ref ID J:83728]

Genotyping

Genotyping Information

Genotyping Protocols

Cd33^tm1Ajv, Separated PCR

Helpful Links

Genotyping resources and troubleshooting

References

References

Selected Reference(s)

Brinkman-Van Der Linden EC; Angata T; Reynolds SA; Powell LD; Hedrick SM; Varki A. 2003. CD33/Siglec-3 Binding Specificity, Expression Pattern, and Consequences of Gene Deletion in Mice. Mol Cell Biol 23(12):4199-206. [PubMed: 12773563]  [MGI Ref ID J:83728]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, these mice can be bred as homozygotes.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location). This strain is included in the Induced Mutant Resource Colony collection.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

See Terms of Use tab for General Terms and Conditions

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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Contact Information
Orders & Technical Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

For Licensing and Use Restrictions view the link(s) below:
- Strain(s) not available to companies or for-profit entities.

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

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