| |||||||||
| MGL1-deficient mice exhibit slightly increased red blood cell counts, mean corpuscular hemoglobin, hematocrit and mean corpuscular volume when compared to wild-type controls. Homozygotes have diminished antigen-induced granulation tissue formation. This mutant mouse strain may be useful in studies of glycosidic molecular interactions and function, antigen-specific and antigen-independent cellular immune response and hematopoiesis. | |||||||||
Type Congenic; Mutant Strain; Targeted Mutation; Mating System Heterozygote x Heterozygote (Female x Male) Species laboratory mouse Generation N11+F (08-OCT-08) Donating Investigator Stephen Hedrick, University of California at San Diego Description
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (mRNA) is detected by Northern blot or RT-PCR analysis. Immunohistochemical reactivity is not detected in inflamed skin. Although mutant mice exhibit slightly increased red blood cell counts, mean corpuscular hemoglobin, hematocrit and mean corpuscular volume when compared to wild-type controls, these levels are within the normal range for mice. Homozygotes have diminished antigen-induced granulation tissue formation but show normal antigen-independent granulation tissue formation. This mutant mouse strain may be useful in studies of glycosidic molecular interactions and function, antigen-specific and antigen-independent cellular immune response and hematopoiesis.This strain was transferred from the collection of the Consortium for Functional Glycomics.
Development
A targeting vector containing a PGKneo casssette was used to disrupt exons 2 and 3, which encode the translational start site and cytoplasmic and transmembrane domains. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+ derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient C57BL/6J blastocysts. The resulting male chimeric animals were crossed to C57BL/6J female mice. Heterozygotes were crossed to obtain homozygotes. The mice were then backcrossed to C57BL/6 for 10 generations before arriving at The Jackson Laboratory.
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Congenic Nomenclature
Mammalian Phenotype Terms assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Mgl1tm1Hed/Mgl1tm1Hed
involves: 129S1/Sv * 129X1/SvJ * C57BL/6
- hematopoietic system phenotype
- abnormal erythrocyte cell number (MGI Ref ID J:78035)
- abnormal mean corpuscular hemoglobin (MGI Ref ID J:78035)
- increased hematocrit (MGI Ref ID J:78035)
- increased mean corpuscular volume (MGI Ref ID J:78035)
- immune system phenotype
- *normal* immune system phenotype (MGI Ref ID J:78035)
- normal thymus, T cell response and macrophage function
Research Applications
This mouse can be used to support research in many areas including:
Cell Biology Research
Protein Processing
Hematological Research
Hematopoietic Defects
Immunology and Inflammation Research
Inflammation
Research Tools
Immunology and Inflammation Research (Macrophage Deficiency)
| Allele Symbol | Mgl1tm1Hed | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Stephen M Hedrick | ||
| Common Name(s) | mMGL-/-; | ||
| Mutation Made By | Peter Sobieszczuk, Consortium for Functional Glycomics,TSRI | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| ES Cell Line Name | R1 | ||
| ES Cell Line Strain | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Gene Symbol and Name | Mgl1, macrophage galactose N-acetyl-galactosamine specific lectin 1 | ||
| Chromosome | 11 | ||
| Gene Common Name(s) | CD301a; Mgl; Mgll; | ||
| Molecular Note | Exons 2 and 3 were deleted by the insertion of a neomycin selection cassette. No transcript was detected in homozygous mutant mice via Northern blot and RT-PCR analyses, however, immunohistochemical staining showed low levels of reactivity in peritoneal exudate cells. [MGI Ref ID J:78035] | ||
Genotyping Protocols
Mgl1tm1Hed, SEP PCR, vers. 1
NEOTD (Generic Neo), STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
Onami TM; Lin MY; Page DM; Reynolds SA; Katayama CD; Marth JD; Irimura T; Varki A; Varki N; Hedrick SM. 2002. Generation of mice deficient for macrophage galactose- and N-acetylgalactosamine-specific lectin: limited role in lymphoid and erythroid homeostasis and evidence for multiple lectins. Mol Cell Biol 22(14):5173-81. [PubMed: 12077344] [MGI Ref ID J:78035]
Mgl1tm1Hed related
Sato K; Imai Y; Higashi N; Kumamoto Y; Onami TM; Hedrick SM; Irimura T. 2005. Lack of antigen-specific tissue remodeling in mice deficient in the macrophage galactose-type calcium-type lectin 1/CD301a. Blood 106(1):207-15. [PubMed: 15784728] [MGI Ref ID J:107475]Sato K; Komatsu N; Higashi N; Imai Y; Irimura T. 2005. Granulation tissue formation by nonspecific inflammatory agent occurs independently of macrophage galactose-type C-type lectin-1. Clin Immunol 115(1):47-50. [PubMed: 15870020] [MGI Ref ID J:105041]
Yuita H; Tsuiji M; Tajika Y; Matsumoto Y; Hirano K; Suzuki N; Irimura T. 2005. Retardation of removal of radiation-induced apoptotic cells in developing neural tubes in macrophage galactose-type C-type lectin-1-deficient mouse embryos. Glycobiology 15(12):1368-75. [PubMed: 16096344] [MGI Ref ID J:133870]
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice can be bred as homozygotes. Diet Information LabDiet® 5K52/5K67
Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $236.40 Female or Male Heterozygous for Mgl1tm1Hed $291.90 Female or Male Homozygous for Mgl1tm1Hed *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $472.80 Heterozygous for Mgl1tm1Hed x Heterozygous for Mgl1tm1Hed
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
|
Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $307.40 Female or Male Heterozygous for Mgl1tm1Hed $379.50 Female or Male Homozygous for Mgl1tm1Hed *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $614.70 Heterozygous for Mgl1tm1Hed x Heterozygous for Mgl1tm1Hed
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
|
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
For additional Licensing and Use Restrictions view the link(s) below:
- Strain(s) not available to companies or for-profit entities.
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form