Strain Name:

B6.129-Mgl1tm1Hed/J

Stock Number:

006944

Availability:

Repository- Live

Use Restrictions Apply, see Purchasing Information
MGL1-deficient mice exhibit slightly increased red blood cell counts, mean corpuscular hemoglobin, hematocrit and mean corpuscular volume when compared to wild-type controls. Homozygotes have diminished antigen-induced granulation tissue formation. This mutant mouse strain may be useful in studies of glycosidic molecular interactions and function, antigen-specific and antigen-independent cellular immune response and hematopoiesis.

Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Mating SystemHeterozygote x Heterozygote         (Female x Male)
Specieslaboratory mouse
GenerationN11+F (08-OCT-08)
 
Donating Investigator Stephen Hedrick,   University of California at San Diego

Description
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (mRNA) is detected by Northern blot or RT-PCR analysis. Immunohistochemical reactivity is not detected in inflamed skin. Although mutant mice exhibit slightly increased red blood cell counts, mean corpuscular hemoglobin, hematocrit and mean corpuscular volume when compared to wild-type controls, these levels are within the normal range for mice. Homozygotes have diminished antigen-induced granulation tissue formation but show normal antigen-independent granulation tissue formation. This mutant mouse strain may be useful in studies of glycosidic molecular interactions and function, antigen-specific and antigen-independent cellular immune response and hematopoiesis.

This strain was transferred from the collection of the Consortium for Functional Glycomics.

Development
A targeting vector containing a PGKneo casssette was used to disrupt exons 2 and 3, which encode the translational start site and cytoplasmic and transmembrane domains. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+ derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient C57BL/6J blastocysts. The resulting male chimeric animals were crossed to C57BL/6J female mice. Heterozygotes were crossed to obtain homozygotes. The mice were then backcrossed to C57BL/6 for 10 generations before arriving at The Jackson Laboratory.

Control Information

  Control
   Wild-type from the colony
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Additional Web Information

Congenic Nomenclature

Phenotype

Phenotype Information

Mammalian Phenotype Terms assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Mgl1tm1Hed/Mgl1tm1Hed

        involves: 129S1/Sv * 129X1/SvJ * C57BL/6
  • hematopoietic system phenotype
  • abnormal erythrocyte cell number (MGI Ref ID J:78035)
  • abnormal mean corpuscular hemoglobin (MGI Ref ID J:78035)
  • increased hematocrit (MGI Ref ID J:78035)
  • increased mean corpuscular volume (MGI Ref ID J:78035)
  • immune system phenotype
  • *normal* immune system phenotype (MGI Ref ID J:78035)
    • normal thymus, T cell response and macrophage function

Research Applications

This mouse can be used to support research in many areas including:

Cell Biology Research
Protein Processing

Hematological Research
Hematopoietic Defects

Immunology and Inflammation Research
Inflammation

Research Tools
Immunology and Inflammation Research (Macrophage Deficiency)

Genes & Alleles

Gene & Allele Information

Allele Symbol Mgl1tm1Hed
Allele Name targeted mutation 1, Stephen M Hedrick
Common Name(s) mMGL-/-;
Mutation Made By Peter Sobieszczuk,   Consortium for Functional Glycomics,TSRI
Strain of Origin(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
ES Cell Line NameR1
ES Cell Line Strain(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Gene Symbol and Name Mgl1, macrophage galactose N-acetyl-galactosamine specific lectin 1
Chromosome 11
Gene Common Name(s) CD301a; Mgl; Mgll;
Molecular Note Exons 2 and 3 were deleted by the insertion of a neomycin selection cassette. No transcript was detected in homozygous mutant mice via Northern blot and RT-PCR analyses, however, immunohistochemical staining showed low levels of reactivity in peritoneal exudate cells. [MGI Ref ID J:78035]

Genotyping

Genotyping Information

Genotyping Protocols

Mgl1tm1Hed, SEP PCR, vers. 1
NEOTD (Generic Neo), STD PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Onami TM; Lin MY; Page DM; Reynolds SA; Katayama CD; Marth JD; Irimura T; Varki A; Varki N; Hedrick SM. 2002. Generation of mice deficient for macrophage galactose- and N-acetylgalactosamine-specific lectin: limited role in lymphoid and erythroid homeostasis and evidence for multiple lectins. Mol Cell Biol 22(14):5173-81. [PubMed: 12077344]  [MGI Ref ID J:78035]

Additional References

Mgl1tm1Hed related

Sato K; Imai Y; Higashi N; Kumamoto Y; Onami TM; Hedrick SM; Irimura T. 2005. Lack of antigen-specific tissue remodeling in mice deficient in the macrophage galactose-type calcium-type lectin 1/CD301a. Blood 106(1):207-15. [PubMed: 15784728]  [MGI Ref ID J:107475]

Sato K; Komatsu N; Higashi N; Imai Y; Irimura T. 2005. Granulation tissue formation by nonspecific inflammatory agent occurs independently of macrophage galactose-type C-type lectin-1. Clin Immunol 115(1):47-50. [PubMed: 15870020]  [MGI Ref ID J:105041]

Yuita H; Tsuiji M; Tajika Y; Matsumoto Y; Hirano K; Suzuki N; Irimura T. 2005. Retardation of removal of radiation-induced apoptotic cells in developing neural tubes in macrophage galactose-type C-type lectin-1-deficient mouse embryos. Glycobiology 15(12):1368-75. [PubMed: 16096344]  [MGI Ref ID J:133870]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX12

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice can be bred as homozygotes.
Diet Information LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Pricing for USA, Canada and Mexico shipping destinations             View   International   Pricing
Weeks of AgePrice*GenderGenotypes Provided
Individual Mouse Price $236.40Female or MaleHeterozygous for Mgl1tm1Hed
$291.90Female or MaleHomozygous for Mgl1tm1Hed
Pairs /Price*Pair Genotype
$472.80Heterozygous for Mgl1tm1Hed x Heterozygous for Mgl1tm1Hed
*Price(s) in US dollars ($)

Supply Details

Standard SupplyRepository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes

Pricing for International shipping destinations             View   USA, Canada and Mexico   Pricing
Weeks of AgePrice*GenderGenotypes Provided
Individual Mouse Price $307.40Female or MaleHeterozygous for Mgl1tm1Hed
$379.50Female or MaleHomozygous for Mgl1tm1Hed
Pairs /Price*Pair Genotype
$614.70Heterozygous for Mgl1tm1Hed x Heterozygous for Mgl1tm1Hed
*Price(s) in US dollars ($)

Supply Details

Standard SupplyRepository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes

Control Information

  Control
   Wild-type from the colony
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
  International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

View JAX® Mice & Services Conditions of Use.

For additional Licensing and Use Restrictions view the link(s) below:
- Strain(s) not available to companies or for-profit entities.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering and Purchasing Information

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Contact Information
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Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
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