Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation F5+N1F1pN1   Donating Investigator Steve Rosen,   University of San Francisco

Description
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (mRNA) is detected by Northern blot analysis of total spleen RNA. Protein activity is not detected in spleen, lung peripheral lymph nodes, mesenteric lymph nodes or Peyer's patches. At 5 to 6 weeks of age, homozygotes have a reduced number (approximately 68% of wildtype levels) of naive T cells in the spleen. Chondroitin sulfate D is not detected in the brains of adult homozygotes or in the telencephalon and cartilage of homozygote embryos aged 12.5 and 15.5 embryonic days. Brain development is not impaired in mutant mice. This mutant mouse strain may be useful in studies of glycosidic molecular interactions and function.

This strain was transferred from the collection of the Consortium for Functional Glycomics.

Development
A targeting vector containing neomycin resistance and diphtheria toxin fragment A genes was used to disrupt the portion of exon 3 that encodes the catalytic domain. The construct was electroporated into 129S2/SvPas derived D3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric animals were crossed to 129/SvJ mice, and then backcrossed to the same for 4 generations before being made homozygous. The mice were then backcrossed to C57BL/6 for 12 generations before arriving at The Jackson Laboratory.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Chst3^tm1Tmu/Chst3^tm1Tmu

        involves: 129S2/SvPas * 129X1/SvJ

• immune system phenotype
• decreased T cell number (MGI Ref ID J:73698)
  • reduction in number of naive T cells in the spleen of 5-6 week old mutant animals
  • however, normal spleen morphology and lymphocyte trafficking into secondary lymph nodes
• nervous system phenotype
• *normal* nervous system phenotype (MGI Ref ID J:73698)
  • normal brain development and morphology, particularly the organization of the cerebral cortex and orientation of processes for pyramidal cells
• hematopoietic system phenotype
• decreased T cell number (MGI Ref ID J:73698)
  • reduction in number of naive T cells in the spleen of 5-6 week old mutant animals
  • however, normal spleen morphology and lymphocyte trafficking into secondary lymph nodes

Genes & Alleles

Gene & Allele Information

Allele Symbol		Chst3tm1Tmu
Allele Name		targeted mutation 1, Takashi Muramatsu
Allele Type		Targeted (knock-out)
Common Name(s)		C6st-;
Mutation Made By		Peter Sobieszczuk,   Consortium for Functional Glycomics,TSRI
Strain of Origin		129S2/SvPas
ES Cell Line Name		D3
ES Cell Line Strain		129S2/SvPas
Gene Symbol and Name		Chst3, carbohydrate (chondroitin 6/keratan) sulfotransferase 3
Chromosome		10
Gene Common Name(s)		C6ST; C6ST-1; C6ST1; GST-0; HSD;
Molecular Note		A neomycin resistance cassette replaced sequences in exon III encoding part of the catalytic domain. Northern blot analysis of spleen did not detect mRNA in homozygous mutant mice. Protein activity was not detected in spleen, lung, and lymph nodes of homozygous mutant mice. [MGI Ref ID J:73698]

Genotyping

Genotyping Information

Genotyping Protocols

Chst3^tm1Tmu, SEP PCR, vers. 1

Helpful Links

Genotyping resources and troubleshooting

References

References

Selected Reference(s)

Uchimura K; Kadomatsu K; Nishimura H; Muramatsu H; Nakamura E; Kurosawa N; Habuchi O; El-Fasakhany FM; Yoshikai Y; Muramatsu T. 2002. Functional analysis of the chondroitin 6-sulfotransferase gene in relation to lymphocyte subpopulations, brain development, and oversulfated chondroitin sulfates. J Biol Chem 277(2):1443-50. [PubMed: 11696535]  [MGI Ref ID J:73698]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, these mice can be bred as homozygotes.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. At least two animals carrying the mutation of interest will be provided. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location). This strain is included in the Induced Mutant Resource Colony collection.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Terms of Use

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