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Genes & Alleles

Chst3;   Chst3tm1Tmu;

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Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Mating System Homozygote x Homozygote         (Female x Male) Species laboratory mouse Donating Investigator Steve Rosen,   University of San Francisco

Strain Description
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (mRNA) is detected by Northern blot analysis of total spleen RNA. Protein activity is not detected in spleen, lung peripheral lymph nodes, mesenteric lymph nodes or Peyer's patches. At 5 to 6 weeks of age, homozygotes have a reduced number (approximately 68% of wildtype levels) of naive T cells in the spleen. Chondroitin sulfate D is not detected in the brains of adult homozygotes or in the telencephalon and cartilage of homozygote embryos aged 12.5 and 15.5 embryonic days. Brain development is not impaired in mutant mice. This mutant mouse strain may be useful in studies of glycosidic molecular interactions and function.

This strain was transferred from the collection of the Consortium for Functional Glycomics.

Strain Development
A targeting vector containing neomycin resistance and diphtheria toxin fragment A genes was used to disrupt the portion of exon 3 that encodes the catalytic domain. The construct was electroporated into 129S2/SvPas derived D3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric animals were crossed to 129/SvJ mice, and then backcrossed to the same for 4 generations before being made homozygous. The mice were then backcrossed to C57BL/6 for 12 generations before arriving at The Jackson Laboratory.

Mammalian Phenotype Terms assigned by genotype The following phenotype information may relate to a genetic background differing from this JAX® Mice strain. Chst3tm1Tmu/Chst3tm1Tmu         involves: 129S2/SvPas * 129X1/SvJ immune system phenotype decreased T cell number (MGI Ref ID J:73698) reduced number of naive T cells in the spleen of 5-6 week old mutant animals nervous system phenotype *normal* nervous system phenotype (MGI Ref ID J:73698) normal brain development and morphology hematopoietic system phenotype decreased T cell number (MGI Ref ID J:73698) reduced number of naive T cells in the spleen of 5-6 week old mutant animals

Gene & Allele Details

Allele Symbol		Chst3tm1Tmu
Allele Name		targeted mutation 1, Takashi Muramatsu
Common Name(s)		C6st-;
Mutation Made By		Peter Sobieszczuk,   Consortium for Functional Glycomics,TSRI
Strain of Origin		129S2/SvPas
ES Cell Line Name		D3
ES Cell Line Strain		129S2/SvPas
Gene Symbol and Name		Chst3, carbohydrate (chondroitin 6/keratan) sulfotransferase 3
Chromosome		10
Gene Common Name(s)		C6ST; C6ST-1; C6ST1; GST-0;
Molecular Note		A neomycin resistance cassette replaced sequences in exon III encoding part of the catalytic domain. Northern blot analysis of spleen did not detect mRNA in homozygous mutant mice. Protein activity was not detected in spleen, lung, and lymph nodes of homozygous mutant mice. [MGI Ref ID J:73698]

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Genotyping Protocols

Chst3^tm1Tmu

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, these mice can be bred as homozygotes.
Diet Information	LabDiet® 5K52/5K67

Additional Web Information

Congenic Nomenclature

Animal Health Reports

Room Number           AX12

Research Applications

This mouse can be used to support research in many areas including:

Cell Biology Research
Protein Processing

Internal/Organ Research
Lymphoid Tissue Defects (T cell deficiency)

References

Selected Reference(s)

Uchimura K; Kadomatsu K; Nishimura H; Muramatsu H; Nakamura E; Kurosawa N; Habuchi O; El-Fasakhany FM; Yoshikai Y; Muramatsu T. 2002. Functional analysis of the chondroitin 6-sulfotransferase gene in relation to lymphocyte subpopulations, brain development, and oversulfated chondroitin sulfates. J Biol Chem 277(2):1443-50. [PubMed: 11696535]  [MGI Ref ID J:73698]

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Price and Supply Information

Strain Name:	B6.129-Chst3tm1Tmu/J
Stock Number:	006945

Price Details

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Supply Details

Standard Supply	Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes	Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection.
Licensing	See General Terms and Conditions below for Licensing and Use Restrictions
Control Information	View Control Information in Strain Details.

General Terms and Conditions

View JAX^® Mice & Services Conditions of Use.

For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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