Strain Name:

STOCK Notch1tm2Rko/GridJ

Stock Number:

006951

Availability:

Repository- Live

Use Restrictions Apply, see Terms of Use

Description

Strain Information

Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Mating SystemHomozygote x Homozygote         (Female x Male)   17-JAN-08
Specieslaboratory mouse
GenerationN1+F4 (23-DEC-08)
 
Donating Investigator Raphael Kopan,   Washington University in St. Louis

Description
Mice homozygous for this "floxed" Notch1 allele (fN1) are viable and fertile. In the targeted allele, loxP sites were placed flanking exon 1 of the targeted gene. When these floxed mice are bred to mice expressing Cre recombinase, exon 1 of the targeted gene is deleted in cre-expressing tissue(s) in the cre-positive, homozygous floxed offspring. These conditional knockout mice may be useful in generating tissue-specific mutants for studying the development of a wide range of tissues: for example, when crossed to a strain expressing Cre recombinase primarily in the nervous system (see Stock No. 003771), this mutant strain may be useful in studies of apoptosis in neural development.

Development
A targeting construct was designed to place a loxP-flanked PGK-neo cassette upstream of exon 1 of the targeted gene, as well as a single loxP site in intron 1. The construct was electroporated into 129X1/SvJ-derived RW4 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a Cre recombinase vector to remove the selection cassette. The resulting ES cells harboring the loxP-flanked exon 1 were injected into blastocysts to generate chimeric mice. Mutant mice were then sent to Dr. Thomas Gridley (The Jackson Laboratory) in 2003 on an unspecified mixed genetic background. These mice were supplied to The Jackson Laboratory Repository in 2007.

Control Information

  Control
   None Available
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Notch1tm2Rko allele
007181   B6.129X1-Notch1tm2Rko/GridJ
View Strains carrying   Notch1tm2Rko     (1 strain)

Strains carrying other alleles of Notch1
002797   B6.129-Notch1tm1Con/J
002445   STOCK Notch1tm1Con/J
006953   STOCK Notch1tm3(cre)Rko/J
View Strains carrying other alleles of Notch1     (3 strains)

Additional Web Information

Introduction to Cre-lox technology

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms
      assigned by genotype

The following phenotype relates to a compound genotype created using this strain.
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Notch1tm2Rko/Notch1tm2Rko Tg(Nes-cre)1Kln/0

        involves: 129X1/SvJ * C57BL/6J * SJL   (conditional)
  • cellular phenotype
  • abnormal apoptosis (MGI Ref ID J:90392)
    • at E10 the total number of apoptotic cells and relative percentage of apoptotic cells to progenitor cells in the forebrain-midbrain junction is significantly reduced compared to littermate controls
  • nervous system phenotype
  • increased neuron number (MGI Ref ID J:90392)
    • the number of postmitotic neurons is increased in the telencephalon at E11.5 - 12.5 compared to littermate controls
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cell Biology Research
Genes Regulating Growth and Proliferation
Signal Transduction

Developmental Biology Research
Growth Defects

Research Tools
Cell Biology Research
Cre-lox System
      loxP-flanked Sequences
Developmental Biology Research
      Cre-lox System

Notch1 related

Developmental Biology Research
Embryonic Lethality (Homozygous)

Genes & Alleles

Gene & Allele Information

 
Allele Symbol Notch1tm2Rko
Allele Name targeted mutation 2, Raphael Kopan
Allele Type Targeted (Floxed/Frt)
Common Name(s) Notch1flox; Notch1tm1Shn; fN1;
Mutation Made By Raphael Kopan,   Washington University in St. Louis
Strain of Origin129X1/SvJ
ES Cell Line NameRW-4
ES Cell Line Strain129X1/SvJ
Gene Symbol and Name Notch1, Notch gene homolog 1 (Drosophila)
Chromosome 2
Gene Common Name(s) 9930111A19Rik; Mis6; Motch A; NOTCH; RIKEN cDNA 9930111A19 gene; TAN1; Tan1; hN1; lin-12; translocation-associated Notch;
Molecular Note LoxP were inserted flanking the first coding exon of the gene. An adjacent loxP flanked neomycin cassette was removed by Cre-mediated recombination in ES cells prior to production of chimeric animals. [MGI Ref ID J:90392]

Genotyping

Genotyping Information

Genotyping Protocols

Notch1tm2Rko, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References

Selected Reference(s)

Yang X; Klein R; Tian X; Cheng HT; Kopan R; Shen J. 2004. Notch activation induces apoptosis in neural progenitor cells through a p53-dependent pathway. Dev Biol 269(1):81-94. [PubMed: 15081359]  [MGI Ref ID J:90392]

Additional References

Notch1tm2Rko related

Bai S; Kopan R; Zou W; Hilton MJ; Ong CT; Long F; Ross FP; Teitelbaum SL. 2008. NOTCH1 regulates osteoclastogenesis directly in osteoclast precursors and indirectly via osteoblast lineage cells. J Biol Chem 283(10):6509-18. [PubMed: 18156632]  [MGI Ref ID J:133664]

Del Barrio MG; Taveira-Marques R; Muroyama Y; Yuk DI; Li S; Wines-Samuelson M; Shen J; Smith HK; Xiang M; Rowitch D; Richardson WD. 2007. A regulatory network involving Foxn4, Mash1 and delta-like 4/Notch1 generates V2a and V2b spinal interneurons from a common progenitor pool. Development 134(19):3427-36. [PubMed: 17728344]  [MGI Ref ID J:128001]

Demehri S ; Kopan R. 2009. Notch signaling in bulge stem cells is not required for selection of hair follicle fate. Development 136(6):891-6. [PubMed: 19211676]  [MGI Ref ID J:146637]

Demehri S; Liu Z; Lee J; Lin MH; Crosby SD; Roberts CJ; Grigsby PW; Miner JH; Farr AG; Kopan R. 2008. Notch-deficient skin induces a lethal systemic B-lymphoproliferative disorder by secreting TSLP, a sentinel for epidermal integrity. PLoS Biol 6(5):e123. [PubMed: 18507503]  [MGI Ref ID J:139386]

Demehri S; Turkoz A; Kopan R. 2009. Epidermal Notch1 loss promotes skin tumorigenesis by impacting the stromal microenvironment. Cancer Cell 16(1):55-66. [PubMed: 19573812]  [MGI Ref ID J:150340]

Huppert SS; Ilagan MX; De Strooper B; Kopan R. 2005. Analysis of Notch Function in Presomitic Mesoderm Suggests a gamma-Secretase-Independent Role for Presenilins in Somite Differentiation. Dev Cell 8(5):677-88. [PubMed: 15866159]  [MGI Ref ID J:98438]

Kiernan AE; Cordes R; Kopan R; Gossler A; Gridley T. 2005. The Notch ligands DLL1 and JAG2 act synergistically to regulate hair cell development in the mammalian inner ear. Development 132(19):4353-62. [PubMed: 16141228]  [MGI Ref ID J:132241]

Lee J; Basak JM; Demehri S; Kopan R. 2007. Bi-compartmental communication contributes to the opposite proliferative behavior of Notch1-deficient hair follicle and epidermal keratinocytes. Development 134(15):2795-806. [PubMed: 17611229]  [MGI Ref ID J:124114]

Pan Y; Lin MH; Tian X; Cheng HT; Gridley T; Shen J; Kopan R. 2004. gamma-secretase functions through Notch signaling to maintain skin appendages but is not required for their patterning or initial morphogenesis. Dev Cell 7(5):731-43. [PubMed: 15525534]  [MGI Ref ID J:94517]

Pan Y; Liu Z; Shen J; Kopan R. 2005. Notch1 and 2 cooperate in limb ectoderm to receive an early Jagged2 signal regulating interdigital apoptosis. Dev Biol 286(2):472-82. [PubMed: 16169548]  [MGI Ref ID J:103603]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX11

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, homozygous mice are bred.
Mating SystemHomozygote x Homozygote         (Female x Male)   17-JAN-08
Diet Information LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Pricing for USA, Canada and Mexico shipping destinations View International pricing
Weeks of AgePrice (US dollars $)GenderGenotypes Provided
Individual Mouse $171.30Female or MaleHomozygous for Notch1tm2Rko
Pairs /Price (US dollars $)Pair Genotype
$342.60Homozygous for Notch1tm2Rko x Homozygous for Notch1tm2Rko

Additional Supply Details

Pricing for International shipping destinations View USA Canada and Mexico pricing
Weeks of AgePrice (US dollars $)GenderGenotypes Provided
Individual Mouse $222.70Female or MaleHomozygous for Notch1tm2Rko
Pairs /Price (US dollars $)Pair Genotype
$445.40Homozygous for Notch1tm2Rko x Homozygous for Notch1tm2Rko

Additional Supply Details

Supply Details

Standard SupplyRepository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement.
Supply Notes

Control Information

  Control
   None Available
 
  Considerations for Choosing Controls
  USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
  International - Control Pricing Information for Genetically Engineered Mutant Strains.

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