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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Generation N1+F0 (01-AUG-07) Donating Investigator Raphael Kopan, Washington Univeristy Description
Mice homozygous for this "floxed" Notch1 allele (fN1) are viable and fertile. In the targeted allele, loxP sites were placed flanking exon 1 of the targeted gene. When these floxed mice are bred to mice expressing Cre recombinase, exon 1 of the targeted gene is deleted in cre-expressing tissue(s) in the cre-positive, homozygous floxed offspring. These conditional knockout mice may be useful in generating tissue-specific mutants for studying the development of a wide range of tissues: for example, when crossed to a strain expressing Cre recombinase primarily in the nervous system (see Stock No. 003771), this mutant strain may be useful in studies of apoptosis in neural development.Development
A targeting construct was designed to place a loxP-flanked PGK-neo cassette upstream of exon 1 of the targeted gene, as well as a single loxP site in intron 1. The construct was electroporated into 129X1/SvJ-derived RW4 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a Cre recombinase vector to remove the selection cassette. The resulting ES cells harboring the loxP-flanked exon 1 were injected into blastocysts to generate chimeric mice. Mutant mice were then sent to Dr. Thomas Gridley (The Jackson Laboratory) in 2003 on an unspecified mixed genetic background. These mice were supplied to The Jackson Laboratory Repository in 2007.
Control Information
| Control | ||
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| None Available | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying Notch1tm2Rko allele
007181 B6.129X1-Notch1tm2Rko/GridJ View Strains carrying Notch1tm2Rko (1 strain)
002797 B6.129-Notch1tm1Con/J 002445 STOCK Notch1tm1Con/J
Additional Web Information
Cre-lox Systems
Phenotype
Phenotype Information
assigned by genotype
The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.Notch1tm2Rko/Notch1tm2Rko Tg(Nes-cre)1Kln/0
involves: 129X1/SvJ * C57BL/6J * SJL (conditional)
- cellular phenotype
- abnormal apoptosis (MGI Ref ID J:90392)
- at E10 the total number of apoptotic cells and relative percentage of apoptotic cells to progenitor cells in the forebrain-midbrain junction is significantly reduced compared to littermate controls
- nervous system phenotype
- increased neuron number (MGI Ref ID J:90392)
- the number of postmitotic neurons is increased in the telencephalon at E11.5 - 12.5 compared to littermate controls
This mouse can be used to support research in many areas including:
Notch1 relatedCell Biology Research
Genes Regulating Growth and Proliferation
Signal Transduction
Developmental Biology Research
Growth Defects
Research Tools
Cell Biology Research
Cre-lox System (loxP-flanked Sequences)
Developmental Biology Research (Cre-lox System)
Developmental Biology Research
Embryonic Lethality (Homozygous)
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Notch1tm2Rko | ||
|---|---|---|---|
| Allele Name | targeted mutation 2, Raphael Kopan | ||
| Allele Type | Targeted (Floxed/Frt) | ||
| Common Name(s) | Notch1flox; Notch1tm1Shn; fN1; | ||
| Mutation Made By | Raphael Kopan, Washington Univeristy | ||
| Strain of Origin | 129X1/SvJ | ||
| ES Cell Line Name | RW-4 | ||
| ES Cell Line Strain | 129X1/SvJ | ||
| Gene Symbol and Name | Notch1, Notch gene homolog 1 (Drosophila) | ||
| Chromosome | 2 | ||
| Gene Common Name(s) | 9930111A19Rik; Mis6; NOTCH; RIKEN cDNA 9930111A19 gene; TAN1; Tan1; hN1; lin-12; translocation-associated Notch; | ||
| Molecular Note | LoxP were inserted flanking the first coding exon of the gene. An adjacent loxP flanked neomycin cassette was removed by Cre-mediated recombination in ES cells prior to production of chimeric animals. [MGI Ref ID J:90392] | ||
Genotyping
Genotyping Information
This strain will not have a genotyping protocol or one is not currently available.
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Yang X; Klein R; Tian X; Cheng HT; Kopan R; Shen J. 2004. Notch activation induces apoptosis in neural progenitor cells through a p53-dependent pathway. Dev Biol 269(1):81-94. [PubMed: 15081359] [MGI Ref ID J:90392]
Additional References
Notch1tm2Rko relatedBai S; Kopan R; Zou W; Hilton MJ; Ong CT; Long F; Ross FP; Teitelbaum SL. 2008. NOTCH1 regulates osteoclastogenesis directly in osteoclast precursors and indirectly via osteoblast lineage cells. J Biol Chem 283(10):6509-18. [PubMed: 18156632] [MGI Ref ID J:133664]
Del Barrio MG; Taveira-Marques R; Muroyama Y; Yuk DI; Li S; Wines-Samuelson M; Shen J; Smith HK; Xiang M; Rowitch D; Richardson WD. 2007. A regulatory network involving Foxn4, Mash1 and delta-like 4/Notch1 generates V2a and V2b spinal interneurons from a common progenitor pool. Development 134(19):3427-36. [PubMed: 17728344] [MGI Ref ID J:128001]
Demehri S; Liu Z; Lee J; Lin MH; Crosby SD; Roberts CJ; Grigsby PW; Miner JH; Farr AG; Kopan R. 2008. Notch-deficient skin induces a lethal systemic B-lymphoproliferative disorder by secreting TSLP, a sentinel for epidermal integrity. PLoS Biol 6(5):e123. [PubMed: 18507503] [MGI Ref ID J:139386]
Huppert SS; Ilagan MX; De Strooper B; Kopan R. 2005. Analysis of Notch Function in Presomitic Mesoderm Suggests a gamma-Secretase-Independent Role for Presenilins in Somite Differentiation. Dev Cell 8(5):677-88. [PubMed: 15866159] [MGI Ref ID J:98438]
Kiernan AE; Cordes R; Kopan R; Gossler A; Gridley T. 2005. The Notch ligands DLL1 and JAG2 act synergistically to regulate hair cell development in the mammalian inner ear. Development 132(19):4353-62. [PubMed: 16141228] [MGI Ref ID J:132241]
Lee J; Basak JM; Demehri S; Kopan R. 2007. Bi-compartmental communication contributes to the opposite proliferative behavior of Notch1-deficient hair follicle and epidermal keratinocytes. Development 134(15):2795-806. [PubMed: 17611229] [MGI Ref ID J:124114]
Pan Y; Lin MH; Tian X; Cheng HT; Gridley T; Shen J; Kopan R. 2004. gamma-secretase functions through Notch signaling to maintain skin appendages but is not required for their patterning or initial morphogenesis. Dev Cell 7(5):731-43. [PubMed: 15525534] [MGI Ref ID J:94517]
Pan Y; Liu Z; Shen J; Kopan R. 2005. Notch1 and 2 cooperate in limb ectoderm to receive an early Jagged2 signal regulating interdigital apoptosis. Dev Biol 286(2):472-82. [PubMed: 16169548] [MGI Ref ID J:103603]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, homozygous mice are bred. Mating System Homozygote x Homozygote (Female x Male) Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $155.70 Female or Male Homozygous for Notch1tm2Rko *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $311.40 Homozygous for Notch1tm2Rko x Homozygous for Notch1tm2Rko
Additional Supply Details
| Supply Notes |
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| Pricing for International shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $202.50 Female or Male Homozygous for Notch1tm2Rko *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $404.90 Homozygous for Notch1tm2Rko x Homozygous for Notch1tm2Rko
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
See Terms of Use
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form
Terms of Use
Terms of Use
General Terms and Conditions
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
Contact information
General inquiries
Contracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice & Services Conditions of Use
“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”No Warranty
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